• Korean Journal of Breeding Science
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• v.40 no.2
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• pp.196-200
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• 2008

A new spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Pinky' was bred from a cross between 'Rima' and 'Yen'. Progenies were investigated from 2004 to 2006 under shading culture in spring and retarding culture in autumn and the promising line was selected based on the performance of year-round production, white rust resistance and vase life. Pinky flowered around 20 October, and year-round production was possible by shading and lighting. Its flower was 3.1 cm in diameter, and had 38.4 petals and 26.8 flowers per stem in autumn. Its petal was narrow (0.4 cm) and pink with green in color at the flower center. Days to flower from short-day start for 'Pinky' was about 47 in spring. 'Pinky' showed a vase life of 20.9 days in autumn. This cultivar was commercially registered in 2007. 'Pinky' would be suitable for culture under the greenhouse and rain shelter condition in Korea.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.541-545
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• 2008

A new spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Yellow Eye' was developed from a cross between 'Angela' and 'Angaesoguk' followed by selections of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 2003 to 2007. Its characteristics were investigated three times from 2005 to 2007 under forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Yellow Eye' was October 24th, but year-round production was possible by shading and lighting. Its flower was 3.3 cm in diameter, and had 31.7 petals and 27.9 flowers per stem in autumn. It has pollenless, single type flowers with yellow petals and green flower center. To flower in under the short day condition, for 'Yellow Eye' was about 41 days in spring, and 'Yellow Eye' showed a vase life of 23.1 days in autumn. This cultivar was registered for a commercialization in 2007. 'Yellow Eye' would be cultured under the greenhouse and rain shelter condition in Korea.

• Horticultural Science & Technology
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• v.30 no.6
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• pp.776-779
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• 2012

A new cultivar 'Yeohong' was originated from an artificial cross between 'Jonathan' and 'Fuji' carried out at National Institute of Horticultural & Herbal Science in 1993. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2002. After regional adaptability test at five districts for subsequent four years as 'Wonkyo Ga-31', it was finally selected in 2007. 'Yeohong' is characterized by the following. Optimum harvest time is late September. Shape of mature fruit at 278 g on mean weight is oblate a light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains favorable total soluble solids at $14.3^{\circ}Brix$ and titratable acidity at 0.4%, which results in a gustatory harmony between sugars and acids. Safe storage period for the fruit is 3 weeks at room temperature. It is not resistant to Bitter rot. 'Yeohong' also reveals a physiological cross compatibility with leading cultivars such as 'Hongro' and 'Tsugaru', but a physiological cross incompatibility with 'Fuji'. It has weak growth habit.

• Korean Journal of Ecology and Environment
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• v.50 no.1
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• pp.116-125
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• 2017

Alpine plants with a scarcity of pollinators in harsh environments have been believed to undergo selfing for reproductive assurance; however, contradictory evidence is also available. Snowmelt regimes in alpine areas function to change life history characteristics of alpine plants such as flowering time and duration; yet the effects of snowmelt regimes have never been tested in alpine plants in Korea. This study was conducted to investigate the dichogamy, mating systems, and flowering characteristics of Megaleranthis saniculifolia populations [early and late snowmelt plots (ESP and LSP, respectively)] in a subalpine area of Sobaeksan Mountain in Korea. M. saniculifolia exhibited incomplete protogyny in that despite early maturation of pistils, maturation times of pistils and stamens within flowers were partly overlapped. Control and hand-outcrossing treatments produced significantly higher number of follicles and seeds per flower than autonomous and hand-selfing treatments. Based on the aggregate fruit set, the auto-fertility index (AI) and self-compatibility index (SI) were 0.33 and 0.50, respectively. Snowmelt occurred 10 days earlier in ESP than in LSP, thereby ESP and LSP showed distinct differences with regard to flower longevity and season, but showing no difference in peak flowering dates. We concluded that M. saniculifolia is an incomplete protogynous and largely outcrossing plant requiring pollinator service. Temporal variation in snowmelt time and subsequent changes in flowering characteristics under climate change may further threaten the population persistence of M. saniculifolia which has already been designated as endangered species in Korea.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.128-132
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• 2013

A new pink gerbera (Gerbera hybrida Hort.) cultivar 'Party Time' was developed from a cross between 'Hope' and 'Sunnyeo', followed by seedling and line selections at the Flower Research Institute of Gyeongnam Agricultural Research and Extension Services (GARES) in 2007. Detailed characteristics of the new cultivar were evaluated from 2005 to 2007. 'Party Time' has semi-double type large flowers in a good harmony with pink (RHS, 65-C) ray floret and brown center. It has a good, stable flower shape and strong peduncles, and its vase life was 12.7 days. The average flower yield of 'Party Time' was about 47.3 stems per plant a year in greenhouse yield trails carried out 2005 to 2007. 'Party Time' (Grant No. 2691) was registered to the Korea Seed and Variety Service (KSVS) for commercialization in 2009.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.14-14
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• 2001

본 연구는 생명공학 관련 기술개발에 의하여 신소재 물질을 생산하고 사람에게 안전하고 생리활성이 높은 고가의 의료용 단백질 생산기술을 체계화하며 형질전환 가축을 이용한 유용물질 생산에 따른 산업화와 부가가치의 극대화에 그 목적이 있다. 유즙으로 사람의 빈혈치료제인 EPO(Erythropoietin)를 분비할 수 있는 형질전환돼지를 생산하기 위하여 WAP(Whey Acidic Protein) Promoter(2.6kb) 하류에 사람의 조혈촉진유전자(EPO: 2.6kb)를 연결시켜 미세주입용 재조합 벡터(WAP-EPO : 약 7.8kb)를 구축하였다. 구축된 재조합 벡터를 1세포기 수정란에 약 2ng/ul 농도로 미세주입한 다음 외과적 방법으로 이식하였다. 이식 후 분만 모돈으로부터 생산된 자돈의 꼬리조직을 이용 게놈DNA를 추출하고 PCR 검정을 한 결과, 유즙으로 사람의 빈혈치료제를 생산할 수 있는 유전자가 도입된 형질전환돼지 $\boxDr$새롬이$\boxUl$ (♂)를 확인하였다. 또한 이렇게 꼬리조직으로부터 확인된 새롬이의 혈액과 정액을 채취, 게놈 DNA를 추출하여 외래 유전자 삽입여부를 PCR 방법으로 검정한 결과 꼬리조직과 마찬가지로 혈액 및 정액에서도 외래유전자가 삽입되었음을 확인할 수 있었다. 이렇게 생산된 형질전환돼지 $\boxDr$새롬이$\boxUl$를 이용 계대번식을 통한 F$_1$ 산자의 생산과 유전자 전이율을 확인하기 위하여 $\boxDr$새롬이$\boxUl$정액을 이용한 인공수정을 실시하였다. 인공수정은 1999년 7월 1일부터 2000년 9월 8일 까지 총 78두의 모돈을 이용하였으며, 그 중 21두의 모돈이 분만하여 인공수정에 의한 분만율은 26.92%로 나타났다.(Table Omitted) Table 1에서와 같이 새롬이 정액을 이용한 인공수정에 의해 형질전환된 F$_1$ 자돈의 형질전환율은 17.98%로 나타났으며, 32두의 형질전환자돈 중 8두(암:4두, 수:4두)는 분만과 동시에 폐사하였거나 사육중 폐사하여 현재 24두(암:12두, 수:12두)가 생존하여 F$_1$ 간 교배계획에 의해 사육되고 있다. 이 중 암컷 4두는 현재 F$_2$ 자돈 생산과 함께 유즙내로 사람 빈혈치료제의 분비 유무를 검정중에 있으며, FISH 법에 의한 외래 유전자 삽입 검정을 확인 중에 있다.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.966-976
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• 2016

A new bright yellow gerbera (Gerbera hybrida Hort.) cultivar, 'Joyful', was released by the Flower Research Institute of Gyeongsangnam-do Agricultural Research & Extension Service in 2013. 'Joyful' was developed in Changwon from 2005 to 2006 and is a cross between 'Chowoo', a semi-double type gerbera cultivar with yellow-orange colored flowers, and 'Focus', a semi-double type cultivar with yellow colored flowers. The characteristics trials for selection were conducted from 2007 to 2009. 'Joyful' has large, semi-double type flowers with yellow (RHS, 12-A) ray florets and a brown central part. It also has a stable flower shape, strong peduncles, and a vase life of 12.8 days. The average flower yield of 'Joyful' was determined in greenhouse trials carried out from 2008 to 2010 and is 49.2 stems per plant per year. 'Joyful' was demonstrated to be a new cultivar based on ploidy tests and RAPD analysis and was registered (Grant No. 4574) to the Korea Seed and Variety Service (KSVS) for commercialization in 2013.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.130-130
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• 2003

번식효율이 높은 우수한 후보종빈돈의 조기선발기술을 개발하기 위해 후보종빈돈의 첫발정일령과 첫수정일령 및 첫수정시 등지방두께가 산자수에 미치는 요인을 분석한 결과는 다음과 같다. 본 시험에 공시된 후보종빈돈은 충남 천안시에 위치하고 있는 양돈장에서 자돈을 생산ㆍ육성하여 체중 80∼90kg에서 선발하였고, 체중이 약 110kg 도달하였을 때 최종 선발하여 이용하였으며, 사양관리는 양돈장의 관행에 따라 실시하였다. 첫발정조사는 생후 22주령때부터 매일 아침, 저녁으로 2회씩 외음부의 충혈과 부종상태를 관찰하고 승가허용 자세유지 등을 통하여 발정여부를 조사하였다. 교배는 첫발정발견 후 2차 발정이 발현되었을 때 실시하는 것을 원칙으로 하였으며, 수퇘지를 허용하는 시기에 액상적액으로 1차 인공수정하고, 12시간후 2차 인공수정을 실시하였다. 첫수정시에 등지방측정기 (Lean-neater; Renco, U.S.A.)를 이용하여 제 10늑골의 정중선으로부터 좌측 또는 우측으로 약 5cm 이격된 지점을 2회 측정하여 평균치로 하였다. 산자수는 미이라 등을 제외하고 정상적으로 성장하여 분만한 복당자돈수를 조사하였다. 첫발정일령이 160일령이하일때에 산자수는 9.64두였으며, 161∼180일렬인 경우 10.14두, 181∼200일령인 경우 9.56두, 201일령이상인 경우 9.13두로 첫발정일령이 161∼180일이었을때가 산자수가 가장 많았으나 유의적인 차이는 없었다. (중략)

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.4
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• pp.455-459
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• 1985

This study is to select good diploid clones and develope them into FDR clones. Seventeen diploid clones were selected by tuber plants from the imported diploid potato seeds and from the progenies of the crosses between them. The characteristics of the selected clones were reported. Fifteen clones were crossed among them or open pollinated or bulk pollinated, and 18 selections were made from the seed progenies of these pollinations. Male and female unreduced gametes were searched by crossing 2x, 4x and by microtechnique. Only one clone, D6-21 was found to produce unreduced male gamete with the rate of 27-30% by FDR. Unreduced female gamete has not been found among these diploids.

• Journal of Korean Society of Forest Science
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• v.89 no.1
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• pp.18-23
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• 2000

Leaves of various, 3 to 5-year-old Quercus hybrids were intermediate in size between their parental species. The petiole length was the smallest in the hybrids of Q. dentata ${\times}$ Q. crispula $F_1$ and was intermediate in the hybrids of Q. aliena ${\times}$ Q. serrata $F_1$ and Q. dentata ${\times}$ Q. aliena $F_1$ between their parents. The number of serration in hybrids was close to their mother tree's in most of crossing combinations. The serration depth and the ratio between longitudinal and transverse length of leaves were intermediate between the values of their parental species.