• Journal of the Korean Institute of Gas
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• v.12 no.2
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• pp.42-47
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• 2008

It is important for national safety that tank lorry or bulk lorry which is carrying toxic gas or explosive materials should be monitored during the maneuvering from starting point to destination as well as vehicle or tank status. Therefore, we propose a safety monitoring system for tank lorry to check driving condition and the vehicle status. We studied the integration of central control system with in-vehicle computer system connected with various sensors for monitoring through a communication network.

• YAKHAK HOEJI
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• v.52 no.3
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• pp.219-224
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• 2008

Clinically isolated methicillin-resistant Staphylococcus aureus strains were exposed to subinhibitory concentration of DW286, DW-224a, gemifloxacin, trovafloxacin, sparfloxacin and ciprofloxacin during 26- to 39-days period. Subculturing led to resistance development, and most of the selected mutants were above susceptible breakpoints. Selected mutants had broad cross resistance to other quinolone antibiotics and only one mutant was completely susceptible to all fluoroquinolones. Twenty five among 42 mutants revealed mutations on DNA gyrase and topoisomerase IV by sequencing. Also 16 mutants had fluoroquinolones MICs that were 4-32 times lower in the presence of reserpine. In conclusion, alterations in DNA gyrase or topoisomerase IV and action of efflux pumping out system are the resistance mechanisms of DW-224a.

• Journal of Institute of Control, Robotics and Systems
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• v.21 no.6
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• pp.503-509
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• 2015

In this paper, we designed and tested an ankle joint mechanism for a gait rehabilitation robot. Gait rehabilitation programs are designed to improve the natural leg motion of patients who have lost their walking capabilities by accident or disease. Strengthening the muscles of the lower-limbs and stimulation of the nervous system corresponding to walking helps patients to walk again using gait assistive devices. It is an obvious requirement that the rehabilitation system's motion should be similar to and as natural as the normal gait. However, the system being used for gait rehabilitation does not pay much attention to ankle joints, which play an important role in correct walking as the motion of the ankle should reflect the movement of the center of gravity (COG) of the body. Consequently, we have designed an ankle mechanism that ensures the safety of the patient as well as efficient gait training. Also, even patients with low leg muscle strength are able to operate the ankle joint due to the direct-drive mechanism without a reducer. This safety feature prevents any possible adverse load on the human ankle. The additional degree of freedom for the roll motion achieves a gait pattern which is similar to the normal gait and with a greater degree of comfort.

• Applied Biological Chemistry
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• v.10
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• pp.33-38
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• 1968

In order to determine proper conditions for the manufacture of canned Kimchi, a heat resistant lactic acid bacteria, the most acid producable strain, was isolated and identified as Lactabacillus plantarum. D-value for the isolate was calculated at 3 from a thermal death curve made at $60^{\circ}C$. The effects of acidity of Kimchi and the amount in a can, and the ratios of solid-liquid, were investigated. The most suitable time for sterilization with a type of can was determined as 25.2 minutes at $85^{\circ}C$. However in the period of heat treatment the quality was badly affected and this fact made desirable introducing of a adequate preservative additives into the manufacture of canned Kimchi.

• Korean Journal of Food Science and Technology
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• v.18 no.5
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• pp.339-344
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• 1986

The specific reaction of trypsin inhibitors with trypsin to form stable complexes was successfully applied for detection of trypsin inhibitors in soybean. Soybean extract was treated with $Ca^{++}$ to remove globulin fraction, followed by digestion with trypsin and fractionated by chromatography on Sephadex G-50. The void volume fraction contained the trypsin-trypsin inhibitor complexes as well as trypsin. The trypsin inhibitors were then detected by their molecular weight differences on SDS-polyacrylamide gel electrophoresis, in which the complexes dissociate into trypsin and its inhibitors. With the method proposed, trypsin inhibitors were indentified by their ability forming the stable complexes with trypsin and their anti-tryptic moiety. The formation of the complexes with trypsin was further confirmed by two dimensional electrophoresis and DEAE-Sephadex A-25 chromatography. Employing the proposed method, it was found that soybean (Glycine max cv. Hill) contained 7 trypsin inhibitors.

• Applied Biological Chemistry
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• v.30 no.1
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• pp.88-94
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• 1987

To investigate ginseng oligopeptides with biological activities, the water extract was purified by ultra-filtration, gel filtration, ion-exchange and thin layer chromatography. Ultra-filtered water extract exhibited antilipolytic activity, inhibiting epinephrine-induced lipolysis in the isolated fat cells of rat epididymal adipose tissue. The filtrate was separated into 3 fractions by Sephadex G-25 gel filtration. Peptides were found only in the first fraction(S-FI). Saponine and sugars were also detected in tie fraction. S-FI fraction resolved further into 6 fractions by Dowex 50 ion-exchange chromatography. The sugar and saponine depleted fraction(P-F2) from the second chromatography showed antilipolytic activity. The P-F2 fraction revealed 6 spots on TLC. The 6 spots were isolated by TLC and identified as peptides.

• Applied Biological Chemistry
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• v.14 no.2
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• pp.109-116
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• 1971

The nutritional status of sulfur in rice plants grown on various field conditions different in soil fertility, fertilizers used and weather was investigated as follows. 1. The critical percentage of sulfur in rice shoot grown in a sulfur deficient field appears to be over 0.2 around 30 days after transplanting. 2. The mean sulfur contents at harvesting stage were 0.22% and 0.43% in the ear and straw, respectively and increased more in the straw by the application of ammonium sulfate. 3. The ratio of sulfur contents in the straw and ear (straw/ear) showed the increasing tendency from north to south only in the fertilized fields. But it was not as such in the percent translocation, the mean values of which were 26% and 29% in the fertilized and non-fertilized, respectively. 4. The sulfur uptake amounted to 6kg and 10kg per acre in the non-fertilized and fertilized, respectively. 5. The sulfur content was decreased in the order of leaf blade+leaf sheath, stem and ear, and was higher in the low productive field than that of high productive field.

• Applied Biological Chemistry
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• v.14 no.2
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• pp.171-175
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• 1971

Gibberella fujikuroi(imperfect stage Fusarium moniforme) a soil fungi is well known as the producer of plant growth regulator Gibberellins. The present work was planned for the isolation of the active strains of Gibberella fujikuroi from the native paddy soils. Twenty two strains were isolated from the infected rice seedlings collected from four local areas. Pyongtaek, Yesan, Tangjin and Sunchon and screened through the activity test for the production of Gibberellins. The strains P-105, Y-14 and T-58 yielded higher activity than the others isolated and the referred strain IAM-8048. The strains Y-5, Y-7, T-54 and S-152, however, were less promotive or rather inhibitory in the growth of rice seedlings. Six different kinds of culture media developed by Cross, Raulin-Thom, Borrow, West, Stodola and Kurosawa respectively were compared with each other for the production of Gibberellins and the best result was obtained with Raulin-Thom's media(glucose 16% and $NH_4NO_3$ 0.24%).

• Journal of Pharmaceutical Investigation
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• v.39 no.6
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• pp.431-435
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• 2009

The comparative superior in vitro activity of DW-224a was supported by the downward MIC distribution due to the weakened influence of alterations within target enzymes in ciprofloxacin-resistant Staphylococcus aureus. The MI$C_{50}$ for DW-224a was 4 $\mu$g/mL, similar to that of gemifloxacin, 8-fold less than that of sparfloxacin and 16-over-fold less than that of ciprofloxacin. We constructed combinations of amino acid changes, located at codon 80, 83 or 84 within GrlA and 84, 85 or 88 within GyrA, which were associated with MIC increase. The amino acid changes were less influential to the MIC of DW-224a compared to those of other fluoroquinolones, and it was verified from the requirement of a total of two GrlA- and two GyrA-alterations to reach the MIC of DW-224a over 32 $\mu$g/mL.

• Applied Biological Chemistry
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• v.51 no.2
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• pp.114-118
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• 2008

Ginseng treated with several treatment conditions of various acids to search hydrolysates on the basis of increased biological activity and modified structure. In the result of acid treatment, the conversion rate of ginsenoside Rg3, Rk1 and Rg5 was highest when ginseng treated with citric acid. After added citric acid to ginseng extract, boiled at l00$^{\circ}C$ for 1 hour and add enzyme, which is examined change by time. It compared with group which did not treated acid. Two groups became difference according to enzyme but the generation rate of ginsenoside Rg3, Rk1 and Rg5 did not show difference greatly. Also, the generation rate of ginsenoside Rg3, Rk1 and Rg5 by time passes did not show difference. The generation rate of ginsenoside Rg3, Rk1 and Rg5 increased when increased acid concentration, temperature and time. We did exclusion molding to shorten treatment time. In the result of ginseng treated with citric acid of various concentrations at various temperatures as time passes by extrusion molding, the generation rate of ginsenoside Rg3, Rk1 and Rg5 was highest when ginseng treated with 3% citric acid at l60$^{\circ}C$ for 20 minutes. In addition, total saponin amount of ginseng treated with 3% citric acid at 160$^{\circ}C$ for 20 minutes was about 11% higher than ginseng heated at 120$^{\circ}C$ for 3 hours. These results indicated that our exclusion molding process more effective, compared to traditional red ginseng manufacturing process.