• Journal of Embryo Transfer
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• v.32 no.3
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• pp.87-94
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• 2017

Tissue engineering (TE) has been developed to create functional organs and tissue by combining 3D matrix and cells in vitro. Vascularization and angiogenesis are utmost important for supply of nutrients and oxygen in tissue engineered organs. The present study was performed to isolate and characterize primary endothelial cells (EC) from aorta of alpha 1, 3-enzyme galactosyltransferase knock out (GalT KO) pig, to minimize immune rejection and analyze body immune system for future xenotransplantation studies. Isolation of primary EC from aorta were performed by incubation with dispase for 8-10 min at $37^{\circ}C$. Primary EC were cultured in EC growth medium on different extra cellular matrix (ECM), either collagen or gelation. Primary EC exhibits morphological characteristics and showed positive expressions of EC specific marker proteins i.e. PECAM1, KDR and VWF despite of their ECM surface; however, on collagen based surface they showed increase in mRNA level analyzed by qPCR. Primary EC cultured on collagen were sorted by flow cytometer using KDR marker and cultured as KDR positive cells and KDR negative cells, respectively. KDR positive cells showed dramatically increased in PECAM1 and VWF level as compared to KDR negative cells. Based on the above results, primary EC derived from GalT KO are successfully isolated and survived continuously in culture without becoming overgrown by fibroblast. Therefore, they can be utilize for xeno organ transfer, tissue engineering, and immune rejection study in future.

• Laboratory Medicine Online
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• v.7 no.3
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• pp.94-102
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• 2017

Malaria, tuberculosis, and hepatitis are common and notorious infectious diseases in Myanmar. Despite intensive efforts to control these diseases, their prevalence remains high. For malaria, which is a vector-borne disease, a remarkable success in the reduction of new cases has been achieved. However, the annual number of tuberculosis cases has increased over the last few decades, and the prevalence of chronic viral hepatitis infection has been high in Myanmar and other nearby countries. Early detection and prompt treatment are crucial to control these diseases. We have devoted our research efforts to understanding the status of these infectious diseases and working towards their eventual elimination for the last four years with the support of the Korea International Cooperation Agency. In the modern era, an infection that develops in one geographical area can spread globally because national borders do not effectively limit disease transmission. Our efforts to understand the status of infectious diseases in Myanmar will benefit not only Myanmar but also neighboring countries such as Korea.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.574-578
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• 2009

A new red leaf lettuce (Lactuca sativa L.) cultivar, "Gopungjeokchukmyeon" which has late bolting and good red expression leaf color was developed from a cross between "Andongjeokchukmyeon" (red leaf color and late bolting) and "Dukseomjeokchukmyeon" (high yield). The cross and selection for advanced lines had been done by the pedigree method during 2000-2006. The advanced lines were evaluated for yield and adaptability at several locations in Korea (Gangwon, Kyunggi, Chungbuk, Kyungnam, Jeonnam, Jeonbuk, and Jeju) from 2007 to 2008. The "Gopungjeokchukmyeon" has gray seed color and traverse elliptic leaves. Compared to "Dukseomjeokchukmyeon", marketable yield of "Gopungjeokchukmyeon" was higher by 17% (at 330 g per plant) and Gopungjeokchukmyeon has particularly improved expression of red leaf color in high temperature cultivation in the field. The shelf-life of "Gopungjeokchukmyeon" was three weeks longer than "Dukseomjeokchukmyeon" at $4^{\circ}C$. The anthocyanin and sugar contents of "Gopungjeokchukmyeon" were higher than those of "Dukseomjeokchukmyeon" with 29.4 mg/100 g and 4.8 brix, respectively. The content anthocynin of "Gopungjeokchukmyeon" is about 10 times higher than those of "seomjeokchukmyeon". Furthermore, its taste is better, more crispy, and sweeter than those of "Dukseomjeokchukmyeon", This new cultivar "Gopungjeokchukmyeon" can be harvested all year around even summer cultivation.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.579-582
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• 2009

A new red leaf lettuce (Lactuca sativa L.) cultivar, "Mihong" which has late bolting and deep red leaf color was developed from a cross of "Danhongongchukmyeon" (deep red but early bolting and low yield) and "Hajicheongchukmyeon" (late bolting and high yield). The selection and breeding of advanced lines were done by pedigree method during 2000-2006. The advanced lines were evaluated for yield and adaptability in Korea (Gangwon, Kyunggi, Chungbuk, Kyungnam, Jeonnam, Jeonbuk, and Jeju) from 2007 to 2008. The "Mihong" has gray seed color, traverse elliptic leaves and deep red color. Compared to "Dukseomjeokchukmyeon", marketable yield of "Mihong" was higher by 2% (at 291 g per plant) as a 17.3 ton/ha, but with particularly improved good expression of deep red leaf color in high temperature cultivation in the field. The shelf-life of "Mihong" was three weeks longer than "Dukseomjeokchukmyeon" at $4^{\circ}C$. The amount of anthocyanin content of "Mihong" were higher than with "Dukseomjeokchukmyeon" at 28.9 mg/100 g. Especially the degree of leaf hardness in "Mihong" showed $26.9kg/cm^2$ compared to "Dukseomjeokchukmyeon". therefore, its taste is better, more crispy, and sweeter than "Dukseomjeokchukmyeon" This new cultivar "Mihong" can be cultivated in all year around even if summer time cultivation.

• Journal of the Korean Society of Marine Environment & Safety
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• v.27 no.6
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• pp.815-821
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• 2021

This study addresses on the design of performance monitoring system for the time synchronization service of the enhanced long-range navigation (eLoran) system, which has a representative ground-wave radio broadcast system capable of providing positioning, navigation, timing and data (PNT&D) services. The limitations of time-synchronized systems due to the signal vulnerabilities of the global navigation satellite system (GNSS) are explained, and the performance monitoring system for the eLoran timing service as a backup to the GNSS is proposed. The time synchronization service using eLoran system as well as system configurations and the user requirements in the differential Loran (dLoran) system are described to monitor the time synchronization performance. The results of the designed system are presented for long-term operation in the eLoran testbed environment. As the results of time performance monitoring, we were able to verify the time synchronization precision within 43.71 ns without corrections, 22.52 ns with corrections. Based on these results, the eLoran system can be utilized as a precise time synchronization source for GPS timing backup.

• The Korea Journal of Herbology
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• v.36 no.3
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• pp.15-24
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• 2021

Objectives : The Glycyrrhiza new varieties, WONGAM and SINWONGAM, were developed through interspecific cross between Glycyrrhiza glabra and Glycyrrhiza uralensis by the National Institute of Horticultural and Herbal Science, Rural Development Administration in Korea. This in vitro study was undertaken to compare the antioxidant and cytotoxic effects between Glycyrrhiza new varieties (WONGAM and SINWONGAM) and official compendia (Glycyrrhiza glabra and Glycyrrhiza uralensis). Methods : Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhy drazyl), ABTS (2,2-azino-bis (3-rthylbenz-thiazoline-6-sulfonic acid)) diammonium salt, Nitrite radical scavenging assay, and Reducing Power assay. Cytotoxicity was determined by MTT assay and cell morphology was observed by an inverted microscope. Results : The DPPH, ABTS, Nitrite radical scavenging activities and reducing power of Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM were evaluated at different concentrations (0, 10, 50, 100, 500, 1000 ㎍/㎖). Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM showed similar dose-dependent increase in antioxidant activities. The cytotoxic effects with increasing doses of Glycyrrhiza new varieties and official compendia did not differ in HCT116, HT29, A549, MDA-MB231, PC3, ACHN, and HeLa cells. However, significant difference in cytotoxicity were observed in AGS, MCF7 and Hep3B cells by Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM. Conclusions : These results showed that Glycyrrhiza new varieties and official compendia acts as a potent antioxidant. Also, the finding that equivalent cytotoxic potency was observed in a cell dependent manner. Our study suggests that Glycyrrhiza new varieties may offer a wide-variety of health benefits.

• Journal of Korean Medicine Rehabilitation
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• v.31 no.2
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• pp.69-79
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• 2021

Objectives The purpose of this study was to verify the effect of integrative Korean medicine on acute postoperative pain after arthroscopic shoulder surgery. Methods This study was conducted in a retrospective observational way. Patients who met the inclusion criteria were divided into integrative Korean medicine-treated group (IKM group) and no integrative Korean medicine treated-group (No IKM group). The primary outcome was the difference in the amount of change in pain intensity. The secondary outcome was the difference in pain intensity, opioid consumption over time, and the number of adverse events after surgery. Results The change of numerical rating scale for pain intensity from baseline to IKM group was 3.09±2.12, while 2.64±1.80 in no IKM group, and the difference of change between two groups was statistically significant (p<0.05). Difference in the use of opioid between two groups over time after surgery was also statistically significant (p=0.000). Conclusions The results of this study suggest that integrative Korean medicine can be effective for acute postoperative pain after arthroscopic shoulder surgery.

• Journal of the Korean Geotechnical Society
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• v.34 no.11
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• pp.81-92
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• 2018

For a marine bridge foundation construction, a large-circular-steel-pipe has been proposed for supporting vertical load and preventing water infiltration. However, a ship collision can adversely affect the structural stability. This paper presents a fundamental study on dynamic responses of the large-circular-steel-pipe by an impact load. In laboratory experiments, small-scaled steel pipe is installed in a soil tank. The soil height and water level are set to 23 cm and 25~70 cm, respectively. The upper part of the steel pipe is impacted using a hammer to simulate the ship collision. The dynamic responses are measured using accelerometers and strain gauges. Experimental results show that the strain decreases as the measured location is lowered. The higher frequency components appear in the impact load condition compared to the microtremor condition. However, the higher frequency components measured at the strain gauge located below the water level do not appear. For the accelerometer signal, the maximum frequency under the impact load is higher than that of the microtremor. The maximum frequency decreases as water level increases but it is larger than the maximum frequency of the microtremor. This study shows that strain gauge and accelerometer can be useful for evaluating the dynamic responses of large-circular-steel-pipes.

• Journal of Wetlands Research
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• v.21 no.1
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• pp.66-76
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• 2019

Recently, natural disasters such as floods and droughts are frequently occurred due to climate change and the damage is also increasing. Wetland is known to play an important role in reducing and minimizing the damage. In particular, water level variability needs to be analyzed in order to understand the various functions of wetland as well as the reduction of damage caused by natural disaster. Therefore, in this study, we fitted water level series of Upo wetland in Changnyeong, Gyeongnam province to a proper time series model and residual test was performed to confirm the appropriateness of the model. In other words, ARIMA model was constructed and its residual tests were performed using existing nonparametric statistics, BDS statistic, and Close Returns Histogram(CRH). The results of residual tests were compared and especially, we showed the applicability of CRH to analyze the residuals of time series model. As a result, CRH produced not only accurate randomness test result, but also produced result in a simple calculation process compared to the other methods. Therefore, we have shown that CRH and BDS statistic can be effective tools for analyzing residual in time series model.

• Korean Journal of Cognitive Science
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• v.30 no.3
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• pp.157-174
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• 2019

Motivation of human individuals to perform on intellectual tasks can be hampered by identity threat from intellectual machines. A laboratory experiment examined whether individuals' performance loss on intellectual tasks appears under human identity threat. Additionally, by affirming alternative attributes of human identity, researchers checked whether group-affirmation alleviate the performance loss on intellectual tasks. This research predicted that under high social identity threat, individuals' performance loss on the intellectual tasks would be moderated by valuing alternative attributes of human identity. Experiment shows that when social identity threat is increased, human individuals affirmed alternative human attributes show higher performance on intellectual tasks than individuals non-affirmed. This effect of human-group level affirmation on performance loss did not appear in the condition of low social identity threat. Theoretical and practical implications were discussed.