• Journal of the Korean Chemical Society
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• v.45 no.3
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• pp.219-222
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• 2001

Separation of lithium isotopes was investigated by chemical ion exchange with a hydrous manganese(IV) oxide ion exchanger using an elution chromatography. The capacity of manganese(IV) oxide ion exchanger was 0.5 meq/g. The heavier lithium isotope was enriched in the solution phase, while the lighter isotope was enriched in the ion exchanger phase. The separation factor was determined according to the method of Glueckauf from the elution curve and isotopic assays. The separation factor of $^6Li^+$-$^7Li^+$ isotope pair fractionation was 1.018.

• Korean Chemical Engineering Research
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• v.52 no.1
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• pp.98-105
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• 2014

Lysozyme amounts to 0.3% in egg white and functions as an agent of cell lysis and activator of tissue reconstruction. Ion exchange chromatography is the most useful method of separation among affinity chromatography, ion exchange chromatography, and ultra-filtration. The aim of present study is to find the optimum pH and temperature for the separation of lysozyme in egg white within cation exchange gel filled glass column. And we compared results of experiments with those of simulations. Phosphate buffer was used, and pH and temperature were varied as 5~7 and $25{\sim}40^{\circ}C$ respectively. RP-HPLC was the tool for the retention time identification and quantitative analysis of lysozyme. OriginPro 8 measured the peak area of lysozyme chromatogram and quantified the eluted lysozyme. Largest amount of lysozyme was separated under the conditions of pH 5 and T $25^{\circ}C$.

• Bioindustry News
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• v.13 no.2
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• pp.13-17
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• 2000

새로운 분리공정으로써 크로마토그래피를 이용하고자 하는 노력들이 활발하게 진행되어지고 있는 가운데, 기존의 전통적인 크로마토그래피를 더욱 발전시킨 시도들이 행하여지고 있다. 여기에 부합하여 약 20여년 전부터 연구되기 시작한 Displacement ion-exchange chromatography는 현재 활발한 연구가 진행되어지고 있으며, 전형적인 preparative chromatography에 비해 많은 장점을 가지고 있다. Displcement ionexchange chromatography는 displacer를 사용하여, 고농도의 원료 물질을 효율적으로 단시간내에 처리할 수 있는 공정으로 기존의 preparative chromatography의 문제점이었던 꼬리끌기 현상과 희시현상을 대폭 감소시킬 수 있게 되었다. 따라서, displacement ion-exchange chromatography 공정에서의 가장 큰 과제인 비선형 등온선 영역에서의 거동과 분리공정 조작선의 결정 및 고성능의 displacer에 대한 연구가 요구되어지고 있으며, 본 총론에서는 이온교환 크로마토그래피 공정에서 displacement ion exchange chromatography를 이용한 단백질의 분리에 대하여 고찰하고자 한다.

• Analytical Science and Technology
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• v.7 no.2
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• pp.201-204
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• 1994

Cation exchange column chromatography of lithium was carried out to investigate the lithium isotope separation in aqueous ion exchange system. A Pyrex glass column of $50cm{\times}6mm$ inner radius with a water jacket was used as the separation column in experiment. Upon column chromatography using hydrochloric and succinic acid mixtures as an elunent, single separation factor, ${\alpha}$, 1.0068 was obtained. From the experiment, it was found that $^6Li$ was enriched in the resin phase and $^7Li$ in the solution phase.

• Korean Chemical Engineering Research
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• v.50 no.4
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• pp.713-717
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• 2012

Approximately forty proteins in egg white have been widely studied for their functional properties. To develop a procedure of separation for pure and non-altered proteins from egg white, purification study was conducted to isolate lysozyme, ovotransferrin, and ovalbumin. Ion exchange cartridge can selectively separate proteins from egg white, and reversed-phase HPLC (RP-HPLC) could identify separated proteins. Proteins in egg white were purified by HI trap ion exchange cartridge SP and Q with buffers pH 8.0 and 5.2. C18 column (Phenomenex, USA) was used for RP-HPLC analysis and isocratic mobile phase was used with acetonitrile (ACN)/distilled water (DW)/trifluoroacetic acid (TFA) in the ratio of 50/50/0.1. Comparing the retention times of standards in RP-HPLC experiments showed that ovotransferrin, ovalbumin, and lysozyme in egg white were eluted successively in the RP-HPLC column after the pretreatment in SP and Q ion exchange cartridges.

• KSBB Journal
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• v.14 no.3
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• pp.279-283
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• 1999

We have compared the breakthrough behavior of lysozyme contained in fresh han egg white on various cation exchagers, and the adsorbent, known by the trade name Cellufine C-200 (Amicon), has shown the best performance. The effects of ion strength, pH, and linear flow rate on the breakthrough behavior were examined using the Cellufine C-200 adsorbent. The optimal conductivity, pH and linear flow rate were determined from the breakthrough behavior and found to be 2.75 mS/cm, 7.0, and 0.635 cm/min, respectively.

• Proceedings of the Membrane Society of Korea Conference
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• 1991.10a
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• pp.45-46
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• 1991

분리막 공정은 상변화를 일으키지 않기 때문에 장치비가 저렴하며, 열 및 화학적으로 민감한 물질, 특히 생체물질 등을 다루는데 적합하다. 또한 최종 생성물질을 정제하는 방법으로는 이온교환 크로마토그래피, 전기 영동 및 친화성 크로마토그래피(affinity chromatography)가 있다. 이중 친화성 크로마토그래피는 가장 선택적이고, 단일 장치로서 기존의 분리방법에 비해 약 1000배 가까이 정제할 수 있다. 본 실험에서는 트립신 억제제인 m-aminobenzamidine을 포함하고 있는 수용성 고분자를 제조하여 트립신을 선택적으로 분리하고자 하였으며, 본 연구 필요한 분리막은 막제조가 용이하며 비용이 저렴한 cellulose acetate막을 사용하였다. CA 막의 세공크기는 겔화매질의 에탄올 농도로 조절하였다. Casting 용액의 조성은 다음 표에 간략하게 나타내었다.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.3 no.4
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• pp.309-317
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• 2005

A study on the separation of $^{99}Tc,\;^{94}Nb,\;^{55}Fe,\;^{90}Sr\;and\;^{59/63}Ni$ in various radioactive wastes discharged from nuclear power plants has been performed for a use in their quantification which is indispensible for the evaluation of the radionuclide inventory Ni was recovered along with Ca, Mg, Al, Cr, Ti, Mn, Ce, Na, K, and Cu through the sequential separation procedure of Re(as a surrogate of $^{99}Tc$), Nb, Fe and Sr by anion exchange and Sr-Spec extraction chromatography. In this research, chemical separation of Ni from the co-existing elements was investigated by cation exchange and Ni-Spec extraction chromatography. Precipitation behaviour of Ni and the co-existing elements with dimethylglyoxime(DMG) was investigated in ammonium $citrate/ethanol-H_2O$ and tartaric $acid/acetone-H_2O$ in order to purify separated Ni fractions and to prepare $^{59/63}Ni$ source for the radioactivity measurement using a gas proportional counter. Recovery of Ni separated through ion exchange chromatographic separation procedure was $92.1\%$ with relative standard deviation of $0.9\%$. In addition, recovery of Ni with DMG in the tartaric $acid/acetone-H_2O$ was $85.6\%$ with relative standard deviation of $1.9\%$.

• KSBB Journal
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• v.18 no.2
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• pp.122-126
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• 2003

Multi-cycle chromatographic separation of Iysozyme from egg white was investigated. Multi-cycle chromatography was performed by repeated cycling(one cycle: resin equilibration, sample loading, washing, elution). Two types of cation exchange resins, Cellufine CM C-200 and Bio-rex 70, were used to determine the optimum condition for the separation of Iysozyme by multi-cycle chromatography. The resin was equilibrated in 20 mM Na-phosphate buffer(pH 7.0). Chromatograms of UV absorbance levels of every cycle were compared to confirm the eluting ability of Iysozyme in the two types of gel. Collected samples from eluting regions in every cycle were assayed by 15% SDS-PAGE.

• Proceedings of the Membrane Society of Korea Conference
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• 1993.10a
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• pp.75-76
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• 1993

자연수에는 무기이온, 용해유기물, 미립자, 미생물과 같은 성분들이 혼재되어 있다. 일반실험실에서 사용하는 증류수, RO수 또는 이온교환수는 실험용 기구 세척수, 일반 정성화학 실험 등에는 적당하지만, 최근 급속히 발전하는 생명공학의 조직배양용이나 PHLC, AAS, 이온크로마토그래피 등 정밀분석기기 용수로는 부적당하다.