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http://dx.doi.org/10.9713/kcer.2012.50.4.713

Identification of Proteins in Egg White Using Ion Exchange Cartridge and RP-HPLC  

Kim, Hyun Moon (Department of Chemical Engineering, Chungnam National University)
Kim, Ah Reum (Department of Chemical Engineering, Chungnam National University)
Lee, Chang Soo (Department of Chemical Engineering, Chungnam National University)
Kim, In Ho (Department of Chemical Engineering, Chungnam National University)
Publication Information
Korean Chemical Engineering Research / v.50, no.4, 2012 , pp. 713-717 More about this Journal
Abstract
Approximately forty proteins in egg white have been widely studied for their functional properties. To develop a procedure of separation for pure and non-altered proteins from egg white, purification study was conducted to isolate lysozyme, ovotransferrin, and ovalbumin. Ion exchange cartridge can selectively separate proteins from egg white, and reversed-phase HPLC (RP-HPLC) could identify separated proteins. Proteins in egg white were purified by HI trap ion exchange cartridge SP and Q with buffers pH 8.0 and 5.2. C18 column (Phenomenex, USA) was used for RP-HPLC analysis and isocratic mobile phase was used with acetonitrile (ACN)/distilled water (DW)/trifluoroacetic acid (TFA) in the ratio of 50/50/0.1. Comparing the retention times of standards in RP-HPLC experiments showed that ovotransferrin, ovalbumin, and lysozyme in egg white were eluted successively in the RP-HPLC column after the pretreatment in SP and Q ion exchange cartridges.
Keywords
Egg White; HPLC; Ion Exchange Chromatography; Cartridge;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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