• Title/Summary/Keyword: 이상저온

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Short-term Hypothermic Preservation of CHO Cells Using Serum-Free Media (무혈청 배지를 이용한 CHO 세포의 단기 저온보존)

  • Byoun, Soon-Hwi;Park, Hong-Woo;Choe, Tae-Boo
    • KSBB Journal
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    • v.21 no.4
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    • pp.306-311
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    • 2006
  • Cell preservation is indispensable in animal cell culture process and should be established according to the cell characteristics. In this study, we experimented hypothermic preservation of CHO cells that is widely used in pharmaceutical industry to produce therapeutic proteins and established a stable method of preservation. The highest viability of CHO cells was obtained when the cells were preserved using rolling tube, which means the cells should be suspended to avoid the cell lumping during the preservation. Also, we obtained superior preservation result under the anaerobic condition. To evaluate the serum-free media as a preservation solution, we investigated cell growth after hypothermic preservation using serum-free media. High cell viability and normal cell growth was observed during 10 days using serum-free media. Moreover, we found that more effective preservation when ${\alpha}$-tocopherol and retinoic acid is added to media as an additive. In the case of 1 liter large scale hypothermic preservation using established protocol, cell viability and growth rate was obtained as good as small scale one. This study is considered to be helpful for hypothermic preservation of CHO cells and large scale hypothermic preservation may be available through the further studies.

에틸렌을 이용한 단일벽 탄소나노튜브의 저온합성

  • Jo, Seong-Il;Jeong, Gu-Hwan
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.337.1-337.1
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    • 2016
  • 단일벽 탄소나노튜브(Single-walled carbon nanotubes, SWNTs)는 나노 스케일의 크기와 우수한 물성을 갖고 있어, 전자, 에너지, 바이오 등 다양한 분야로의 응용이 기대되고 있다. 이러한 응용의 실현을 위해서는 경제적, 산업적인 면에서 보다 손쉬운 합성법이 요구된다. SWNTs의 합성에는 대면적의 균일한 CNTs를 합성할 수 있다는 장점이 있는 열화학기상증착법(Thermal chemical vapor deposition, TCVD)이 가장 일반적으로 사용되고 있다. 하지만 탄화수소가스를 효율적으로 분해하기 위하여 $900^{\circ}C$ 이상의 고온공정이 요구되며, 이는 경제적, 산업적인 면에서 사용이 제한적이다. 따라서 저결함, 고수율의 SWNTs를 저온합성 할 수 있는 공정의 개발이 지속적으로 필요하다. 본 연구에서는, TCVD법을 이용하여 에틸렌 원료가스로 SWNTs의 저온합성 가능성을 확인하였다. 합성을 위한 기판과 촉매로는 실리콘 산화막 기판(SiO2/Si wafer)에 철 나노입자를 지닌 ferritin을 스핀코팅 후 산화하여 이용하였다. 저온합성 공정의 변수로는 합성온도와 원료가스인 에틸렌의 분율을 설정하여, 변수가 SWNTs의 결정성과 수율에 미치는 영향을 고찰하였다. 합성된 SWNTs의 분석의 용이함과 손지기(Chirality)의 제어 가능성을 확인하기 위하여 나노 다공성 물질인 제올라이트(Zeolite)를 보조 기판으로 사용하였다. 실험결과 에틸렌 원료가스로 합성한 SWNTs는 메탄을 원료가스로 사용한 경우보다 낮은 $700^{\circ}C$ 부근에서도 합성이 가능함을 확인하였다. 또한 에틸렌의 분율과 합성 시간의 정밀한 제어를 통해 SWNTs의 합성온도를 더욱 감소시키는 것도 가능할 것으로 예상된다.

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Change of Diurnal Respiration and Transpiration Rate of Fruits in Kiwifruit during Fruit Growth (참다래 착과 과실의 호흡과 증산속도의 일변화)

  • Han Sang-Heon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.8 no.3
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    • pp.152-158
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    • 2006
  • The objective of this study is to investigate change in diurnal respiration and transpiration of the fruits of kiwifruit during fruit growth. Three-hourly fruit transpiration and respiration rate were measured by a chamber technique. Results showed a tendency of higher transpiration and respiration in at maturation to commercial harvest period in 1995 fruit than in 1996 fruit. Fruit respiration rates were very similar to the transpiration rates. The air temperature record for the fruit maturation period in 1996 showed a sudden drop on September $19{\sim}24$ and October 14 down to $7{\sim}13^{\circ}C$. These results suggest that abnormal fruit transpiration and respiration rate in the fruit maturation period might be influenced by the air temperature.

Meteorological Constraints and Countermeasures in Rice Breeding -Breeding for cold tolerance- (기상재해와 수도육종상의 대책 - 내냉성품종육성방안-)

  • Mun-Hue Heu;Young-Soo Han
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.27 no.4
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    • pp.371-384
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    • 1982
  • Highly cold tolerant varieties are requested not only at high latitute cool area but also tropical high elevated areas, and the required tolerance is different from location to location. IRRI identified 6 different types of cold tolerance required in the world for breeding purpose; a) Hokkaido type, b) Suweon type, c) Taipei 1st season type, d) Taipei 2nd season type, e) Tropical alpine type and, f) Bangladesh type. The cold tolerance requested in Korea is more eargent in Tongil group cultivars and their required tolerance is the one such as the physiological activities at low temperature are as active as in Japonica group cultivars at least during young seedling stage and reproduction stage. With conventional Japonica cultivars, such cold tolerant characters are requested as short growth duration but stable basic vegetative growth, less sensitive to high temperature and less prolonged growth duration at low temperature. The methods screening for cold tolerance were developed rapidly after the Tongil cultivar was reliesed. The facilities of screening for cold tolerance, such as, low temperature incubator, cold water tank, growth cabinet, phytotron, cold water nursery in Chuncheon, breeding nursery located in Jinbu, Unbong and Youngduk, are well established. Foreign facilities such as, cold water tank with the rapid generation advancement facilities, cold nurseries located in Banaue, Kathmandu and Kashimir may be available for the screening of some limitted breeding materials. For the reference, screening methods applied at different growth stages in Japan are introduced. The component characters of cold tolerance are not well identified, but the varietal differences in a) germinability, b) young seedling growth, c) rooting, d) tillering, e) discolation, f) nutrition uptake, g) photosynthesis rate, h) delay in heading, i) pollen sterility, and j) grain fertility at low temperature are reported to be distinguishable. Relationships among those traits are not consistent. Reported studies on the inheritance of cold tolerance are summarized. Four or more genes are controlling low temperature germinability, one or several genes are controlling seedling tolerance, and four or more genes are responsible for the pollen fertility of the rice treated with cold air or grown in the cold water nursery. But most of those data indicate that the results may come out in different way if those were tested at different temperature. Many cold tolerant parents among Japonicas, Indicas and Javanicas were identified as the results of the improvement of cold tolerance screening techniques and IRTP efforts and they are ready to be utilized. Considering a) diversification of germ plasm, b) integration of resistances to diseases and insects, c) identification of adaptability of recommending cultivars and, d) systematic control of recommending cultivars, breeding strategies for short term and long term are suggested. For short term, efforts will be concentrated mainly to the conventional cultivar group. Domestic cultivars will be used as foundation stock and ecologically different foreign introductions such as from Hokkaido, China or from Taiwan, will be used as cross parents for the adjustment of growth durations and synthsize the prototype of tolerances. While at the other side, extreme early waxy Japonicas will be crossed with the Indica parents which are identified for their resistances to the diseases and insects. Through the back corsses to waxy Japonicas, those Indica resistances will be transfered to the Japonicas and these will be utilized to the crosses for the improvement of resistances of prototype. For the long term, efforts will be payed to synthsize all the available tolerances identified any from Japonicas, Indicas and Javanicas to diversify the germ plasm. The tolerant cultivars newly synthsized, should be stable and affected minimum. to the low temperature at all the growing stages. The resistances to the diseases and insects should be integrated also. The rapid generation advancement, pollen culture and international cooperations were emphasized to maximize the breeding efficiency.

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Effects of Low Air Temperature and Light Intensity on Yield and Quality of Tomato at the Early Growth Stage (정식 초기의 저온·저일조가 토마토 수량·품질에 미치는 영향)

  • Wi, Seung Hwan;Yeo, Kyung-Hwan;Choi, Hak Soon;Yu, Inho;Lee, Jin Hyong;Lee, Hee Ju
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.448-454
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    • 2021
  • This study was conducted to the effect of low air temperature and light intensity conditions on yield and quality of tomato at the early stage of growth in Korea. Inplastic greenhouses, low temperature and low temperature with shade treatments were performed from 17 to 42 days after plant. Tomato growing degree days were decreased 5.5% due to cold treatment during the treatment period. Light intensity decreased 74.7% of growing degree days due to shade. After commencing treatments, the plant growth decreased by low temperature and low radiation except for height. Analysis of the yield showed that the first harvest date was the same, but the yield of the control was 3.3 times higher than low temperature with shade treatment. The cumulative yields at 87 days after transplanting were 1734, 1131, and 854 g per plant for control, low temperature, and low temperature with shade, respectively. The sugar and acidity of tomatoes did not differ between treatment and harvesting season. To investigate the photosynthetic characteristics according to the treatment, the carbon dioxide reaction curve was analyzed using the biochemical model of the photosynthetic rate. The results showed that the maximum photosynthetic rate, J (electric transportation rate), TPU (triose phosphate utilization), and Rd (dark respiration rate) did not show any difference with temperature, but were reduced by shading. Vcmax (maximum carboxylation rate) was decreased depending on the low temperature and the shade. Results indicated that low temperature and light intensity at the early growth stage can be inhibited the growth in the early stage but this phenomenon might be recovered afterward. The yield was reduced by low temperature and low intensity and there was no difference in quality.

Magnetic Properties of BiPbSrCaCuO System (Bi계 초전도체의 자기적 효과)

  • 이상헌
    • Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
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    • 2002.02a
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    • pp.355-357
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    • 2002
  • The relationship between magnetic properties of BiPbSrCaCuO superconductor and externally applied magnetic field was studied to develop a magnetic field polarity sensor. The behavior was related to the magnetic flux trapped in the superconductor, which penetrates through the material by the external magnetic field. Some portion of the superconductor was changed to a normal state by the trapped magnetic flux. Electrical characteristics of the superconductor with trapped magnetic flux were extremely sensitive to the external magnetic field and showed different responses depending on the direction of the magnetic field.

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A Study on the Quench characteristics Analysis considering Ac-Losses of High-Tc Superconducting Synchronous Motor (고온초전도동기모터의 교류손실에 따른 ?치 해석에 관한 연구)

  • 윤용수;송명곤;문창욱;홍계원;이상진;고태국
    • Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
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    • 1999.02a
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    • pp.93-96
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    • 1999
  • This paper deals with the quench characteristic analysis in high-Tc superconducting synchronous motor of five to six hundred watts capacity. First, the magnetic field distribution and ac losses of high-Tc superconducting motor with the additive flux damper circuit was derived and computer simulation was performed with the equivalent model using FEM. The simulation results show that the quench state lasts for about 0.3 seconds.

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농업기술 - 시설감자, 온도와 토양수분관리 중요!

  • Jang, Dong-Chil;Park, Jong-Yun
    • 농업기술회보
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    • v.50 no.2
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    • pp.36-37
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    • 2013
  • 우리나라에서 감자는 거의 연중 생산되며, 최근 저온저장고 등의 산지유통시설이 보급되면서 홍수출하에 따른 가격폭락 현상이 줄어들고 있다. 특히 겨울철 시설감자는 재배하기 쉽고 단경기에 출하되기 때문에 소득 작목으로 크게 부각되고 있으며, 그 면적은 약 1.500ha에 이른다. 그러나 이상기상에 따른 저온피해, 동해, 일조부족 등은 시설감자 생산에 종종 피해를 주고 있으므로, 온도와 토양수분, 광도를 최적 상태로 유지하는 것은 시설감자 생산의 기본적인 요소이다.

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Inductance Calculation with OPERA program between Sections of MRI Magnet (OPERA를 이용한 MRI 마그네트의 권선간 인덕턴스 계산)

  • 배준한;심기덕;고락길;진홍범;권영길;류강식;이상진
    • Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
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    • 2000.02a
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    • pp.173-176
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    • 2000
  • MRI magnet has generally multi-section coil configurations to generate highly homogeneous magnetic field. Each coil is bridged by a shunt resistor to protect the superconducting magnet during quench. In order to optimize the shunt resistor, self inductance of each coil and mutual inductances between coils should be determined beforehand. Therefore, we calculated the self and the mutual inductances of MRI magnet with OPERA program for electromagnetic analysis.

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Homogeneous Magnet Design Technique Using Evolution Strategy (진화알고리즘을 이용한 균즐자장 발생용 마그네트의 최적설계)

  • 송명곤;김동훈;이상진
    • Progress in Superconductivity and Cryogenics
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    • v.4 no.1
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    • pp.114-118
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    • 2002
  • We introduce a design technique for homogeneous magnets using evolution strategy. The method has several advantages over existing techniques including: it allows complete flexibility in geometric constraints on the shape of both the coil and the homogeneous volume; it guarantees a globally optimal solution, and it automatically searches the minimum number of coils that satisfies given constraints.