• 식물조직배양학회지
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• 제24권6호
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• pp.361-367
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• 1997

담배(Nicotiana tabacum cv. BY4)의 이배체식물과 담배의 약으로부터 유도된 반수체식물의 잎절편을 사용하여 2.4-D를 단독톄처리하여 캘러스를 유도하였다. 캘러스증식은 이배체에서는 2.0 mg/L 2.4-D와 1.0mg/L 카이네틴의 조합처리구에서, 반수체의 경우에 있어서는 2.0mg/L 2.4-D와 0.1mg/L BAP 조합처리구에서 실시하였으며, 현탁배양도 동일조건으로 실시하였다. 현탁배양 세포괴를 배양 15-20일째가 되면 0.8% low melting agarose와 혼합하여 카드a뮴이 0-2,000 $\mu$M의 농도로 처리된 선발배지에 평판배양하여 카드뮴저항성 colony(cell clump)를 조사관찰하였다. 그 결과 본 실험에서는 이배체는 세포의 성장에 있어서 최하저해농도(MIC : Minimum Inhibition Concentration)는 Cd$^{++}$ 300 $\mu$M이었으며 반수체는 Cd$^{++}$ 200 $\mu$M이었다. 이때 반수체 및 이배체의 MIC에서 선발된 세포주를 카드뮴 500, 1,000, 2,000 $\mu$M 농도로 옳겨 경화시킨 후 카드뮴저항성을 선발하였고 그 특성을 조사하였다. 그 결과 카드뮴 저항성을 띤 반수체 및 이배체유래 캘러스의 경우에는 각각 카드a뮴의 농도가 증가할수록 생중량은 감소하였으나, 건조중량은 오히려 증가하였다. 그리고 각각 선발된 세포주내의 카드뮴의 함량을 AAS를 사용하여 측정하였던 바, 카드뮴의 농도가 높을수록 캘러스에 축적된 카드뮴의 양이 높게 나타났다.

• 미생물학회지
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• 제17권3호
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• pp.131-136
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• 1979

충치 sample 50개에서 46 strains의 Streptococcus sp.를 분리하여 그 생리적 특징을 조사한 바 1. 대부분의 균주들이 non-haemolytic strain이었으며, 2. 기질로 maltose, inulin, mannitol, sorbisI, lactose, mannabinose를 분해하여 산을 생성하고 arabinose와 xylose는 균주에 따라 차이를 보였으며, 3. Sucrose broth에서는 거의 gel을 형성하였다.

• 미생물학회지
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• 제2권1호
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• pp.25-27
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• 1964

Lee, Bae Ham, Rha, Min Keun and Choi, Tae joo (Dept. of Biology, Kon Kuk University). Identification of the causal organism of cereal scab in Korea. Kor. J. Microbiol. Vol. 2, No. 1, p. 25-27 (1964) Head blight or scab occurred in barley, wheat, rye and some other cereals widely in this country during the spring of 1963. The causal organisms were collected from 34local areas and isolated purely. All isolates identified as Gibberella zeae (Schw.) Petch. and Fusarium graminearum Schw. as conidial stage.

• 미생물학회지
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• 제19권1호
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• pp.3-7
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• 1981

Attempts were made to develope the method of biological control by application of hyperparasitism on plant disease. The hyperparasitic fungi used in this work was Trichoderma sp. which was isolated from the ginseng growing soil, and the host fungi were Fusarium oxysporum Schlecht and Glomerella cingulata(St) Spau. et Schr. The hyperparasitic fungi identified as Trichoderma viride. It was observed that the hyperparasitic fungi either contact and penetrate into the hyphae of the host or inhibit the growth of host finally destroy of the host cells.

• 식물조직배양학회지
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• 제28권1호
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• pp.37-40
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• 2001

벼의 약배양 배지 내에 콜히친 첨가에 의한 염색체 배가 방법을 확립하기 위하여, 콜히친의 농도를 0.001∼0.1 mg/L까지 조절하여 약배양하였을 때 콜히친의 농도가 증가할수록 캘러스 형성률과 식물체 분화율은 점점 감소하였으며, 그 감소폭은 농도가 증가됨에 따라 점점 커지는 경향이었다. 자포니카 3품종과 통일형 1품종을 콜히친이 첨가되지 않은 배지에 약배양하였을 때 반수체의 출현빈도가 50%이상 높게 나타난 반면, 이배체는 40% 이하의 낮은 발생빈도를 나타내었다. 그러나 0.001 mg/L의 콜히친이 약배양 배지에 첨가될 경우 품종에 따라 다소간의 차이는 있으나, 반수체의 출현빈도는 상대적으로 감소되고 이배체의 발생 비율이 54.3∼60%로 증가하였다.

• 미생물학회지
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• 제17권2호
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• pp.65-71
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• 1979

On studying of microbes and appearance frequency which are floating around dwelling space and life environments in Seoul area, the results are summarized as follows ; 1. 1931 strains of 29 bacteria are isolated in dwelling spaces, Among them, there are Staphylococcus aureus, S. anthracis which are pathogenic to human beings. 2. In fungi, 76 strains of 13 species are isolated. That of highest frequency is confirmed to be Aspergillus which is pathogens of Aspergillosis (one of a group of diseases of animals and human being caused by various species of Aspergillus). The places, where the number of kinds and the frequency of appearances have been observed highest, are coffee shops, lunch counters and office rooms, while factory districts are of far lower level than expected. 3. 0.1% $HgCl_2$, Leasking oil, and Telephone disinfectant were better than any other in fungicidal effect.

• 미생물학회지
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• 제18권1호
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• pp.1-6
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• 1980

Five species of food decay fungl, Aspergillus flavus, Asp. uiger, Penicillum sp., Botrytis cinerea, and Rhizopus stolonifer, were examined for their radio sensitivity in several suspension media. Asp. flavus, Asp. niger and Penicillum sp. have almost the same sensitivity toward gamma rays. with D value in the range of 30 to 35 K rad, whereas Botrytis cinerea has a D value of approximately 55K rad and Rhizopus stolonifer, the most re4sistant fungus studied, has a crease in their radioresistnace when compared with spores irradiated in water. Asp. flavus and penicillium sp. spores irradiated in citrate buffer at pH3-7 showed almost no change in their radisensitiity with pH, but Botryis cinerea spores showed a distinct decrease in their radioresistnace at pH 6 and 7. Penicillum sp. spores irradiated in sucrose solutions showed no sinificant change in their radioresistance. Botrytis cinerea spores displayed a higher radioresistance when they were irradiated in sucrose solution than in water.

• 미생물학회지
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• 제6권1호
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• pp.1-5
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• 1968

This experiment was undertaken the purpose of investigating the difference of the content of free amino acids in the diseased and healthy leaves of the resistance and susceptible rice varieties, to blast by means of paper chromatography. The results obtained are summarized as follows. (1) In the healthy and diseased leaves, the former was founded nine to tell sorts of free amino acids and later was observed eleven. In the both of leaves, alanine and valine were contained in larger amount, and aspartic acid, glycine and arginine were found relatively less in general. (2) In the diseased leaves, the amino acids were increased in quantity, especially glutamine and aspartic acid were remarkable, and cystine were found only in the diseased ones. (3) It seems to that the health leaves in all varieties were observed the amino acid contents, in notably increase and decrease, but in the diseased ones were not changed in amount. Tatacho, susceptible variety was more contained the amount of glutamine and aspartic acid than Zenith, resistance one.

• 한국연초학회지
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• 제16권2호
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• pp.108-112
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• 1994

Response of two ploidy levels to cool temperature and short day treatment were investigated under controlled conditione of Phytotron. The haploid and diploid of seven genotypes were started and grown to the 8- leaf stage in the greenhouse. They were treated during 15 and 20 days to 8- hour photoperiods at 18$^{\circ}C$ in controlled - environmental room to induce premature flowering, respectively. Diploid plants of seven genotypes flower later than their haploid plants at 20 days treatment. But under 15 days treatment, diploid plants of NC82, Hicks, BY4, NC2326 and Coker86 were not different from their haploid plants for days to flower. Diploid plants of seven genotypes developed the same number of leaves as their haploid plants at 20 days treatment. Under 15 days treatment, diploid plants of Coker347 and NC95 developed more leaves per plant than their haploid plants. Correlation coefficient between the ranks of leaves per plant of seven genotypes at two ploidy levels was 0.964 and 0.929 at 15 and 20 days treatment, respectively.

• 생약학회지
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• 제10권3호
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• pp.112-118
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• 1979

21 herbal drugs registered in K.P. III were tested for contamination of fungi and isolation of aflatoxin producible strains. Initially contaminated fungi were Aspergillus group (41.28%) and Penicillium (47.26%) and the other fungi were contaminated somewhat. The most frequent isolation of Aspergillus group was Cnidii Rhizoma and that of Penicillium was Piperis Fructus nigri. Cnidii Rhizoma was the most contaminated drug and Cassiae Cortex was the least among them. Aspergillus flavus was isolated from 10 samples and Aspergillus parasiticus was detected in Glycyrrhizae Radix, Phellodendri Cortex. Aspergillus ochraceus was isolated from only Scutelariae Radix, and Fusarium nivale was isolated from Cnidii Rhizoma and Torreya Semen. None of Aspergillus and Penicillium was detected in only Coptidis Rhizoma. No strains of Aspergillus flavus and Aspergillus parasiticus isolated from were produced aflatoxin.