• Title/Summary/Keyword: 이동성 분석

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Characterization of Ferritin Isolated from Dog Spleen (개의 비장에서 분리한 페리틴의 특성)

  • Park Jae-Hag;Jun Do Youn;Kim Young Ho
    • Journal of Life Science
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    • v.15 no.3 s.70
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    • pp.439-446
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    • 2005
  • Ferritin is known to be the principle iron-storage protein in a wide variety of rganisms. The electro­phoretic mobility and immunological cross-reactivity of dog splenic ferritin were compared with those of horse, bovine, and pig splenic ferritin after isolation using heat treatment, salting out, column chromatography, and ultrafiltration. These isolation methods allowed the recovery of $\~84{\mu}g$ of the ferritin per g of spleen. The iron content in the dog ferritin was $22.7\%$, which appeared to be higher than those in the other mammalian ferritins tested. The electrophoretic mobility of the dog ferritin under nondenaturing conditions was similar to its bovine counterpart, whereas it was more identical to pig and horse ferritins on an SDS-polyacrylamide gel. The molecular weight of the dog ferritin subunit was 19.5 kDa on an SDS-polyacrylarnide gel, and the subunit was unable to bind with iron. The polyclonal anti-dog ferritin raised in rats was able to cross-react with the pig, bovine, and horse ferritins, upon Ouchterlony double immunodiffusiion. A Western blot analysis also revealed that the anti-dog ferritin, which specifically bound with the dog ferritin subunit, could also recognize the horse, bovine, and pig ferritin subunits and the maximum cross-reactivity was exhibited with the pig ferritin subunit, indicating that the dog ferritin is immunochemically more similar to the pig ferritin than its other mammalian counterparts. Accordingly, these results elucidate the biochemical and immunochemical characteristics of dog ferritin that might have a potential to be applied as an oral iron supplement to treat iron deficiency anemia.

Outbreak of Scion Root from 'Shiranuhi Mandarin' Hybrid Tree in Plastic Film House (부지화 감귤에서 자근의 발생)

  • Kang, Seok-Beom;Moon, Young-Eel;Lee, Dong-Hoon;Kim, Yong-Ho;Han, Seung-Gab;Chae, Chi-Won
    • Korean Journal of Environmental Agriculture
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    • v.31 no.4
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    • pp.313-317
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    • 2012
  • BACKGROUND: Citrus is usually propagated by grafting onto a rootstock. In Korea, As trifoliate orange (Poncirus trifoliata) has dwarf and strong cold hardness, it is widely used as the rootstock of satsuma mandarin. Because 'Shiranuhi' ((Citrus unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulata), a kind of citrus, also, generally is grafted onto a trifoliate orange, most of farmer has been recognized that 'Shiranuhi' root is naturally trifoliate orange. Meanwhile, reduction of flowering in 'Shiranuhi' orchard has been issued among the farmers and researchers over past few years and they guessed it was occurred by severe prune, oversupply of fertilization, overfruiting and temperature of growth period. However, a few researchers strongly assumed that it would be caused by scion rooting of 'Shiranuhi'. So, this study was carried out to identify the existence of scion rooting in 'Shiranuhi' tree in Korea. METHODS AND RESULTS: To identify the existence of scion rooting in 'Shiranuhi' tree, we randomly selected six 'Shiranuhi'orchards and we surveyed three to four trees, which flowering was not enough, from six 'Shiranuhi' orchards respectively. We took the root samples of 'Shiranuhi' mandarin, and then separated the two group which were non-scion rooting (Trifoliate orange), and scion rooting ('Shiranuhi' mandarin). To identity the scion rooting, we used primer set of three types which were a F2/R15, F4/R15 and F5/R15 primer set. As a result, when we conducted the DNA analysis, fourteen tree in less bloomed twenty tree was proved as tree with the scion rooting of 'Shiranuhi' mandarin. CONCLUSION(S): Scion roots of 'Shiranuhi'mandarin were usually observed in a deeply planted tree, and xylem of 'Shiranuhi' root indicated more white color than a case of trifoliata orange. 'Shiranuhi' tree by scion rooting was more vigorous but less flowering than trees grafted onto trifoliata orange. When we used F2/R15, F4/R15 and F5/R15 primer set for discriminance of 'Shiranuhi'mandarin root and trifoliate root, we identified the existence of scion rooting in 'Shiranuhi', From our results, it is suggested that the influence of scion root should be reviewed in 'Shiranuhi'orchards.

The Growth Characteristic of the Main Afforestation Species Using the Change of the Annual Ring in Uiseong Area (연륜 변화를 이용한 의성지역 주요 조림수종의 생장특성)

  • Lee Dong-Sup;Kim Dong-Geun;Bea Kwan-Ho;Suh Hyoung-Min
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.7 no.4
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    • pp.274-281
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    • 2005
  • The climate of the Uisong Area is wet in the heat of summer and cold and dry in winter, The climate is highly consistent. The typical forest soil of the Uisong Area has an acidity of pH 4.5-5.6. The thickness of soil A layers is thin to an average of 10-20 cm and is a typical brown forest soil type. The growth characteristic of this climate and soil environment is as follows. In the case of Larix leptolepis, it takes 5-9 years to reach 6 cm diameter, The annual growth rate falls off greatly at an age of 17-19 years. The change of annual rings is more apparent on the north slopes compared with the east and northeast slopes. Pinus rigida takes 7-9 years to reach a 6 cm diameter. Annual growth rates of Pinus rigida fall greatly when age reaches 19 years. The change of annual ring growth of Pinus rigida was most apparent in the west and northeast slopes compared with the south and east slopes. In the case of Pinus koraiensis, the change of the annual ring width according to the characteristics of the slope is not important. Pinus koraiensis takes 6 yearsto reach the 6cm diameter, and annual growth rate fell off at age 19 years. In the case of Quercus acutissima, the growth of the valley trees is more stable than for trees grown at the mountain base. Annual growth rate of Quercus autissima fell off 19 years after planting. Ring growth of Robinia pseudo-acasia on northeast slopes appears much like that on northwest slopes. In conclusion, the main silviculture species reaches a 6 cm diameter in 5-9 years of growth, and the annual growth rate begins to fall between years 13 and 19 in the Uisong Area.

Comparison between Conservative Treatment of partial ACL Rupture and Reconstructive Surgery with BPTB Autograft in ACL Rupture (전방 십자 인대 파열후 자가 슬개건을 이용한 재건술군과 부분 손상후 보존적 치료군의 비교)

  • Lee Dong Chul;Lee Su Ho;Kim Dong Han
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.1 no.2
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    • pp.131-137
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    • 2002
  • Purpose: To evaluate and compare the functional results, activity status, and anterior stability between conservative group with partial ACL injury and reconstruction group with bone-patellar ten-don-bone autograft in ACL total rupture. Materials and Methods: Thirty-eight patients with ACL injury were diagnosed and treated with arthroscopy and followed for more than two years. The number of patients with partial injury was 12(mean age; 38.3) and reconstruction group was 26 (mean age; 25.3). Objective stability was estimated under anterior loading of 15 lb and 20 lb by KT-2000 Arthrometer (MED metric, USA). Functional evaluation using Lysholm score and Tegner activity score were performed. Results: Average functional score of Lysholm was 88.5 in partial injury group and 89.3 in reconstruction group. Average score of Tegner was 5.3 in partial injury group and 5.1 in reconstruction group(P<0.05).Average anterior displacement compared with normal side. Under loading of 20lb, 1.7$\pm$4.3 mm in partial injury group, 2.3$\pm$1.9 mm in reconstruction group were anterior displaced (P<0.05). Under loading of 15lb, 1.2$\pm$1.0 mm in partial injury group, 1.4$\pm$1.5 mm in reconstruction group were dis-placed (P<0.05). There were giving way, effusion, instability and anterior knee pain in complication. Giving way was the frequent complication in the partial injury group. Conclusions: Clinical results of both group were similar. Functional evaluation of Lysholm was good and status of Tegner activity was maintained to physical fitness activites (jogging, regular bik-ing) in both groups on average. The results of conservative treatment for the partial ACL injury (less than 50$\%$) was satisfactory and equivalent to that of reconstructive treatment for the total ACL injury.

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Results of Open Versus Arthroscopic Method in Recurrent Anterior Shoulder Instability (관혈적 방법과 관절경적 방법을 이용한 재발성 견관절 전방 불안정의 치료 결과)

  • Hahn Sung-Ho;Yang Bo-Kyu;Yi Seung-Rim;Chung Shun-Wook;Lee Dong-Ho;Oh Se-Jin;Lee Chul-Ho;Ha Kwon-Ick
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.1 no.2
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    • pp.154-158
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    • 2002
  • Purpose: To compare the results of open and arthroscopic method in recurrent anterior should erinstability. Materials and Methods: The 68 patients who have been taken the open or arthroscopic Bankartrepair for the period of Jan. 1995 to April. 2000. One group (23 patients) had elected an arthroscopic Bankart repair, the other group (45 patients) had chosen open stabilization. Patients were followed up12 to 63 months (ave. 34 months) after surgery. Results: We found 2 cases of subluxation out of open repair group, and then treated by conservative method. There were another 2 cases of dislocation and 2 cases of subluxation out of arthroscopicrepair group, and we have taken out 1 case of reoperation by open method. Using the functional scales by Rowe, the patients who have taken the open method posted at the average point of 87, while the arthroscopic method posted 85 points. Patients satisfaction points were 84.6 and 72.5 respectively. There were no criteria of statistically significant except stability and motion score. Conclusion: Open Bankart repair would be better in stability, and arthroscopic method in ROM gain. Proper patient selection based on physical examination and arthroscopic inspection to optimize the indications contributed to successful treatment.

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Comparison of Student Satisfaction with Foodservice of Middle School by Place for Meal Service in Busan Area (배식장소에 따른 부산 일부지역 중학생의 급식만족도 평가)

  • Lee, Kyung-A;Lyu, Eun-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.2
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    • pp.209-218
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    • 2005
  • The purpose of this study was to evaluate the students' satisfaction with the Quality of middle school foodservice in Busan area. For purpose, Questionnaires were distributed to 788 students in 8 middle schools (four schools for classroom service, four schools for restaurant service). The average scores were -1.13 for gap of meal, -1.68 for sanitation, -1.19 for menu, -1.63 for attitude of foodservice staffs, and -0.89 for rapidity and exactness of service. The whole students assessed the important and performance of school food service 4.20/5.00 and 2.99/5.00. The restaurant service had significantly (p<0.01) higher average scores performance of meal, sanitation, and rapidity and exactness of service than those of the class room service. The Importance-Performance Analysis (IPA) gride, high importance and low performance (focus here) were cleanliness of dish, cleanliness restoration area, rapid treatment about complaints, reflection students' opinions, and listening to students' opinions in the classroom service and the restaurant service, but the Quality vs. price of meal, sanitation of meal, and dessert supply were included in focus here part in the classroom service.

Dental Hygienists' Turnover Intention and its Related Factors (치과위생사의 이직요인에 대한 조사연구)

  • Yoon, Mi-Sook;Lee, Kyung-Hee;Choi, Mi-Sook
    • Journal of dental hygiene science
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    • v.6 no.1
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    • pp.11-17
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    • 2006
  • The purpose of this study was to help prevent the turnover of competent dental hygienists in a bid to boost the efficiency of personnel management for dental health care workers and provide higher-quality oral health services. After relevant literature and data were reviewed, a survey was conducted on dental hygienists, who worked at dental institutes, for approximately four months from September to December 2004 to identify what affected their turnover. The findings of the study were as below: 1. Regarding turnover experience, 39.7 percent of the dental hygienists investigated had such an experience. As to turnover frequency, those who took up another employment once made up the largest group(28.2%), followed by twice(8.0%) and three times(2.9%). The most dominant turnover reason was working conditions(66.7%), followed by seeking being hired by larger institutes(36.2%), pay(21.7%), relationship with dentists(11.6%) and commuting distance(11.6%). 2. As for their hope for turnover, 82.8 percent hoped to take up another employment, and working conditions were cited as the most common reason(44.4%), followed by pay(33.3%), commuting distance(18.1%), marriage(13.2%), health/use of leisure time(11.8%), and commuting time(10.4%). 3. Concerning preference for future workplace, 38.5 percent, the largest group, wanted to work at public health clinics. As to a preferred term of working as dental hygienists, 50.0 percent, the greatest group, hoped to serve as dental hygienists until they are financially secure. 34.5 percent, the second largest group, intended to keep working until they reach the age limit. In regard to their responsibility for family economy, 47.7 percent, the greatest percentage, shouldered the partial responsibility for that, and 31.6 percent assumed no responsibility. 4. As to their intention to quit working as dental hygienists, 61.5 percent were willing to do that, and marriage(29.0%) was singled out as the most frequent reason, followed by working conditions(27.1%), child birth(22.4%), health/housework(18.7%), pay(15.9%) and learning/use of free time(15.0%).

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Supplemental Lighting by HPS and PLS Lamps Affects Growth and Yield of Cucumber during Low Radiation Period (약광기 HPS와 PLS lamp를 이용한 오이의 보광재배효과)

  • Kwon, Joon-Kook;Yu, In-Ho;Park, Kyoung-Sub;Lee, Jae-Han;Kim, Jin-Hyun;Lee, Jung-Sup;Lee, Dong-Soo
    • Journal of Bio-Environment Control
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    • v.27 no.4
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    • pp.400-406
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    • 2018
  • In this experiment the effect of supplemental lighting on the growth and yield of cucumber (Cucumis sativus L. 'Fresh') plants during low radiation period of winter season were investigated in glasshouses using common high-pressure sodium (HPS) lamps and newly developed plasma lighting system (PLS) lamps. Plants grown without supplemental lighting were considered as a control. Supplemental lighting was provided from November 20th, 2015 to March 15th, 2016 to ensure 14-hour photoperiod (natural+supplemental light), also lamps were operated automatically when the outside sun radiation levels were less than $100W{\cdot}m^{-2}$. Spectral analysis showed that HPS lamp had a discrete spectrum, lacked of the radiation in the 400-550 nm wave band (blue-green light), but had a high output in the orange-red region (550-650 nm). A higher red light output resulted in an increased red to far-red (R/FR) ratio in HPS lamp. PLS had a continuous spectrum and had a peak radiation in green region (490-550 nm). HPS has 12.6% lower output in photosynthetically active radiation (PAR) but 12.6% higher output in near infra-red (NIR) spectral regions compared to PLS. Both HPS and PLS lamps emitted very low levels of ultra-violet radiation (300-400 nm). Supplemental lighting both from HPS and PLS lamps increased plant height, leaf number, internode number and dry weight of cucumber plants compared to control. Photosynthetic activity of cucumber plants grown under two supplemental lighting systems was comparable. Number of fruits per cucumber plant (fruit weight per plant) in control, PLS, and HPS plots were 21.2 (2.9 kg), 38.7 (5.5 kg), and 40.4 (5.6 kg), respectively, thereby increasing yield by 1.8-1.9 times in comparison with control. An analysis of the economic feasibility of supplemental lighting in cucumber cultivation showed that considering lamp installation and electricity costs the income from supplemental lighting increased by 37% and 62% for PLS and HPS lamps, respectively.

Development of Industrial Embedded System Platform (산업용 임베디드 시스템 플랫폼 개발)

  • Kim, Dae-Nam;Kim, Kyo-Sun
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.47 no.5
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    • pp.50-60
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    • 2010
  • For the last half a century, the personal computer and software industries have been prosperous due to the incessant evolution of computer systems. In the 21st century, the embedded system market has greatly increased as the market shifted to the mobile gadget field. While a lot of multimedia gadgets such as mobile phone, navigation system, PMP, etc. are pouring into the market, most industrial control systems still rely on 8-bit micro-controllers and simple application software techniques. Unfortunately, the technological barrier which requires additional investment and higher quality manpower to overcome, and the business risks which come from the uncertainty of the market growth and the competitiveness of the resulting products have prevented the companies in the industry from taking advantage of such fancy technologies. However, high performance, low-power and low-cost hardware and software platforms will enable their high-technology products to be developed and recognized by potential clients in the future. This paper presents such a platform for industrial embedded systems. The platform was designed based on Telechips TCC8300 multimedia processor which embedded a variety of parallel hardware for the implementation of multimedia functions. And open-source Embedded Linux, TinyX and GTK+ are used for implementation of GUI to minimize technology costs. In order to estimate the expected performance and power consumption, the performance improvement and the power consumption due to each of enabled hardware sub-systems including YUV2RGB frame converter are measured. An analytic model was devised to check the feasibility of a new application and trade off its performance and power consumption. The validity of the model has been confirmed by implementing a real target system. The cost can be further mitigated by using the hardware parts which are being used for mass production products mostly in the cell-phone market.

Annealing Effects on Properties of ZnO Nanorods Grown by Hydrothermal Method (수열합성법으로 성장된 산화아연 나노막대의 특성 및 열처리 효과)

  • Jeon, Su-Min;Kim, Min-Su;Kim, Ghun-Sik;Cho, Min-Young;Choi, Hyun-Young;Yim, Kwang-Gug;Kim, Hyeoung-Geun;Lee, Dong-Yul;Kim, Jin-Soo;Kim, Jong-Su;Lee, Joo-In;Leem, Jae-Young
    • Journal of the Korean Vacuum Society
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    • v.19 no.4
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    • pp.293-299
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    • 2010
  • Vertically aligned ZnO nanorods on Si (111) substrate were prepared by hydrothermal method. The ZnO nanorods on spin-coated seed layer were synthesized at $140^{\circ}C$ for 6 hours in autoclave and were thermally annealed in argon atmosphere for 20 minutes at temperature of 300, 500, $700^{\circ}C$. The effects of the thermal annealing on the structural and optical properties of the grown on ZnO nanorods were investigated by X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM), photoluminescence (PL). All the ZnO nanorods show a strong ZnO (002) and weak (004) diffraction peak, indicating c-axis preferred orientation. The residual stress of the ZnO nanorods is changed from compressive to tensile by increasing annealing temperature. The hexagonal shaped ZnO nanorods are observed. The PL spectra of the ZnO nanorods show a sharp near-band-edge emission (NBE) at 3.2 eV, which is generated by the free-exciton recombination and a broad deep-level emission (DLE) at about 2.12~1.96 eV, which is caused by the defects in the ZnO nanorods. The intensity of the NBE peak is decreased and the DLE peak is red-shifted due to oxygen-related defects by thermal annealing.