• Journal of Adhesion and Interface
• /
• v.10 no.2
• /
• pp.77-83
• /
• 2009

PDMS was selected for a coating material of implantable biosensors and the cytotoxicity of extracts released from a polymer was evaluated using ISO 10993-5, Biological evaluation of medical devices-Part 5: Tests for in vitro cytotoxicity. Organo-tin was used as a positive control and a medium without serum was used as a negative control. Materials extract were prepared by incubating specimens in RPMI medium without serum ($125{\mu}L/cm^2$) for 24 h, 1 week and 6 weeks at $38^{\circ}C$. The evaluation of cytotoxicity was performed by two different methods : 1) seeding cells with extracts at the beginning 2) incubating extracts with cell sheets already formed on the plate. Both cell morphology and MTT numerical data were shown for the confirmation of cytotoxicity and cell spreading on the surface of PDMS.

• Journal of Life Science
• /
• v.18 no.2
• /
• pp.287-290
• /
• 2008

In order to obtain yeast cells producing a bacteriocin, Subpeptin JM4-A or Subpeptin JM4-B, the 48 bp oligonucleotides corresponding to Subpeptin JM4-A and Subpeptin JM4-B genes including codon for start and stop were chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Escherichia coli and Pseudonomas aeruginosa. This result indicates that yeast cells producing Subpeptin JM4-A or Subpeptin JM4-B possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative E. coli and P. aeruginosa cells. The recombinant yeast strains constructed in this study can be applied in the food preservative or. animal foodfeed.

• Korean Journal of Food Science and Technology
• /
• v.38 no.6
• /
• pp.799-804
• /
• 2006

Antioxidative and antimicrobial activities were measured for the Pleurotus eryngii (P. eryngii) solvent extracts in order to discover new functional activities. In P. eryngii, the powder moisture was 9.0%, and the carbohydrate, crude protein, crude ash and crude fat contents were 63.06, 20.70, 5.20 and 2.0%, respectively. Among the detected minerals, potassium (K) had the highest levels and manganese (Mn) the lowest. The amount of polyphenol in EtEx (Ethanol Extract) was 387 mg% for the whole body, 158 mg% for the stipe, and 593 mg% for the pileus. Higher levels of polyphenol in the entire body were found in the BuEx (Butanol Extract) (594 mg%) and WaEx (Water extract) (404 mg%) of the P. eryngii powder. BuEx had the highest level in the pileus, and EtEx and BuEx were higher than the other extracts in the stipe. The electron donating ability (EDA) of EtEx of the P. eryngii powder was the highest, at 91.12%, for the whole body, while it was the lowest, at 62.90%, in the stipe. In addition, the EDA of WaEx was 90.39% for the whole body. These EDA values were similar to those for tocopherol (93.93%) and BHT (96.72%), supporting the potential of these extracts to act as antioxidants. A number of the extracts were certified to have antimicrobial activities for small number of microorganisms, especially for gram-negative microorganisms. In other words, BuEx and EAEx in the pileus and WaEx in the stipe were found to inhibit the growth of Pseudomonas aeruginosa (gram negative). Additionally, EtEx and WaEx in all parts were shown to act as antimicrobial agents for Escherichia coli of gram negative.

• Polymer(Korea)
• /
• v.31 no.1
• /
• pp.14-19
• /
• 2007

Demineralized bone particle (DBP) has been used as one of the powerful inducers of bone and cartilage tissue specialization. In this study, we fabricated DBP/PLGA scaffold for tissue engineered disc regeneration. We manufactured dual-structured scaffold to compose inner cylinder and outer doughnut similar to nature disc tissue. The DBP/PLGA scaffold was characterized by porosity, wettability, and water uptake ability. We isolated and cultured nucleus pulposus (NP) and annulus fibrosus (AF) cells from rabbit intervertebral disc. We seeded NP cells into the inner core of the hybrid scaffold and AF cells into the outer portion of it. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole-2-yl) -2,5- diphenyltetrazolium -bromide (MTT) test. PLGA and PLGA/DBP scaffolds were implanted in subcutaneous of athymic nude mouse to observe the formation of disc-like tissue in vivo. And then we observed change of morphology and hematoxylin and eosin (H&E). Formation of disc-like tissue was better DBP/PLGA hybrid scaffold than control. Specially, we confirmed that scaffold impregnated 20 and 40% DBP affected to proliferation of disc cell and formation of disc-like tissue.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.2 no.4
• /
• pp.545-551
• /
• 1998

The Radio communication by existing SSB, VHF, etc. in a coast station and a base station for military affairs is still used to a very important the device of information delivery or transmitting and receiving by the remote controller to using to the exclusive cable for a equipment established at a long distance. When a number of consumer to connected and operated by a number of transceiver is essential for a circuit controller of ICS, in KOREA, is devoted by import to considerable quantity because of to be delayed development of this field. This Paper has been realized to optimal algorithm and designing of a circuit connection controller by multi-processor to pre-stage for the development of ICS. The H/W is composed able to remote control to circuit connector with the several slave processor and a processor for master, and this has taken possible through without any obstacle to communication circuits of a control signal by FSK system. The S/W make possible monitoring for communication condition of other circuits by means of a serial communication system by the multi-processing. This paper has been studied for connecting to a circuits wished to rapidly and precisely by the full application to a interrupt technique. A technique to control by remote to a number of transceiver is a way increasing to application for a frequency resource of the limited MF/SF, VHF and the existing radio communication technique. According to, this paper will achieve to be the reduction of energy & equipment and multiplicity of information delivery in the general communication and disposal to rapid and exact for the important communication as distress, urgency and safety on the sea.

• Microbiology and Biotechnology Letters
• /
• v.36 no.2
• /
• pp.101-105
• /
• 2008

In order to obtain yeast cells producing a bacteriocin OR-7, the 180 bp polynucleotide corresponding to the OR-7 gene including codons for start and stop was chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Campylobacter jeuni, Escherichia coli and Pseudomonas aeruginosa. This result indicates that yeast cells producing OR-7 possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative C. jejuni, E. coli and P. aeruginosa cells. The recombinant yeast strain constructed in this study can be applied in the food preservative or animal feed.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.23 no.2
• /
• pp.118-151
• /
• 1997

Allergic contact dermatitis is a common skin disease resulting from specific immumologic sensitization to topically applied various allergen. Purpose of this study was to investigate skin morphologic chganges by light microscopic, changes of protein band by SDS-PAGE in the skin treated on the surfactant. Furthermore, lectin histochemistry is used to know the chagnges of the terminal sugar of the glycoconjugate in the skin treated on the surfactant. The results were as follows : The 1 day of treated group was indicated the enlargement of capillary, the 3 days of treated group was showed that the cytoplasm was eosinophlic by the pyknotic of nucleus. The 6 days of treated group was observed hyperkeratinoid, increased of inflammation cells to epithelium. The 9 days of treated group was appeares that serum crust was sheded and formed a acanthosis. But dermis layer was cytoplasmic vaculation and enlargement of intercellular space. The 12 days treated group made up new epidermis layer of seven layer and of observed an decreased in quantity of inflammation cells. The mast cell of degranulated type was increased in treated surfactnat. It was secreating granules hold histamin, serotonin and heparin. Accordingly, protein band of electrophortic phase was observed a sudden changes since 3 days treated group. At observeation of the cell-surface glycoconjugates, LCA, PNA, SBA and WGA lectin positive cells but inducated lectin negative cells in Con-A. Furthermore, PNA and SBA showed stroger positive reaction as treated surfactant group.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.33 no.4
• /
• pp.269-273
• /
• 2007

We studied anti-microbial and anti-oxidant activities of bio-active components in stem of Juniperus chinensis L. and leaf of Abies koreana Wilson. Those plants of needle leaf tree family were reported to contain anti-cancer compounds. The anti-bacterial activity was tested by Broth dilution method against Escherichia coli and Staphylococcus aureus. As results, Juniperus chinensis L. and Abies koreana Wilson extracts showed 17.0% and 8.5% higher anti-bacterial activity than methyl paraben, respectively. The free radical scavenging activity of Juniperus chinensis L. and Abies koreana Wilson extracts showed 45 % and 44 % at 5,000 ppm. We measured polyphenol (catechin equivalent) and flavonoids quantity. The Juniperus chinensis L. extract contained 312 mg/g of polyphenol and 105 mg/g of flavonoids. The Abies koreana Wilson extract contained 280 mg/g of polyphenol and 103.8 mg/g of flavonoids. The cytotoxicity of extracts was measured by neutral red assay. Extracts did not affect the viability of CCK-986sk cells up to a concentration of 1,250 ppm. In conclusion, these data suggest that extracts of needle leaf trees would be usefull as antiseptic agents and anti-oxidants for cosmetic products.

• Proceedings of the Korea Society for Simulation Conference
• /
• 2002.05a
• /
• pp.125-129
• /
• 2002

실시간 운영체제(Real-Time Operating System: 이하 RTOS라 함) 개발환경에서 제공하는 도구 중에 하나인 RTOS 시뮬레이터는 타겟 하드웨어가 호스트에 연결되어 있지 않아도 호스트에서 응용프로그램의 개발과 디버깅을 가능하게 해주는 타겟 시뮬레이션 환경을 제공해 줌으로서, 개발자로 하여금 빠른 시간 내에 응용프로그램을 개발할 수 있도록 지원하며 하드웨어 개발이 완료되기 전에도 응용프로그램을 개발할 수 있게 해 준다. 그러한 이유로 현재 대부분의 상용 RTOS 개발환경에서는 RTOS 시뮬레이터를 제공하고 있다. 그러나 현재 상용 RTOS 시뮬레이터들은 대부분 RTOS의 기능적인 부분들만 호스트에서 동작하도록 구현되어 있어서 RTOS나 RTOS 응용프로그램이 실제 타겟에서 실행될 때의 실질적인 시간 추정이 불가능하다. 이러한 문제점은 실시간 시스템이 정해진 시간 내에 결과를 출력해야 하는 시스템임을 감안한다면 RTOS 시뮬레이터의 가장 큰 결점이 되기 때문에 실행시간 추정 기능을 가지면서 실용화도 가능한 RTOS 시뮬레이터가 필요하다. 본 연구에서는 이러한 문제점을 해결하여 RTOS와 RTOS 응용프로그램이 실제 타겟에서 처리될 때의 실행시간 추정이 가능하고 상용화가 가능한 기계 명령어 기반(machine instruction-based)의 RTOS 시뮬레이터를 연구 개발하였다. 나아가 실행시간의 주요 요소인 파이프라인과 캐쉬의 영향도 고려함으로서 실행시간 추정의 정확도를 향상시켰다 본 연구에서 사용된 RTOS는 한국전자통신연구원(ETRI)에서 2000년에 개발된 Q+이고, Q+가 동작하는 타겟 하드웨어는 ARM 계열의 StrongARM SA-110 마이크로프로세서와 21285 주제어기가 장착된 EBSA-285 보드이다. 측정하면서 수행하였다. 검증 결과 random 상태에서는 문헌자료에 부합되는 예측결과를 보여주었으나, intermediate와 constant 상태에서는 문헌보다 다소 낮은 속도를 보여주었다 이러한 속도차는 추후 현장 데이터를 수집하여 보다 실질적인 검증을 통하여 조정되어야 할 것으로 판단된다.지발광(1.26초)보다 구애발광(1.12초)에서 0.88배 감소하였고, 암컷에서 정지발광(2.99초)보다 구애발광(1.06초)에서 0.35배 감소하였다. 발광양상에서 발광주파수는 수짓의 정지발광에서 0.8 Hz, 수컷 구애발광에서 0.9 Hz, 암컷의 정지발광에서 0.3 Hz, 암컷의 구애발광에서 0.9 Hz로 각각 나타났다. H. papariensis의 발광파장영역은 400 nm에서 700 nm에 이르는 모든 영역에서 확인되었으며 가장 높은 첨두치는 600 nm에 있고 500에서 600 nm 사이의 파장대가 가장 두드러지게 나타났다. 발광양상과 어우러진 교미행동은 Hp system과 같은 결과를 얻었다.하는 방법을 제안한다. 즉 채널 액세스 확률을 각 슬롯에서 예약상태에 있는 음성 단말의 수뿐만 아니라 각 슬롯에서 예약을 하려고 하는 단말의 수에 기초하여 산출하는 방법을 제안하고 이의 성능을 분석하였다. 시뮬레이션에 의해 새로 제안된 채널 허용 확률을 산출하는 방식의 성능을 비교한 결과 기존에 제안된 방법들보다 상당한 성능의 향상을 볼 수 있었다., 인삼이 성장될 때 부분적인 영양상태의 불충분이나 기후 등에 따른 영향을 받을 수 있기 때문에 앞으로 이에 대한 많은 연구가 이루어져야할 것으로 판단된다.태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$

• Journal of the Korean Wood Science and Technology
• /
• v.38 no.4
• /
• pp.341-348
• /
• 2010

Antifungal and antibacterial activities of wood vinegars were investigated in this study in order to apply for the natural fungicides and food preservatives. The antifungal activities of wood vinegars were measured as a hyphal growth inhibition rate using four plant pathogenic, four wood rot and one mushroom pathogenic fungi. Inhibition effect on the fungi growth was explicitly observed at the concentrations higher than 0.5% of unrefined wood vinegar and higher than 1.0% of refined wood vinegar, respectively, suggesting that unrefined wood vinegars showed the higher antifungal activities than that of refined wood vinegars. The highest inhibition effect on the fungi growth was shown in Libertella betulina which is a mushroom pathogenic fungus. In addition, the wood vinegars from Pinus densiflora showed the higher antifungal activities against the plant pathogenic and wood rot fungi than that from Quercus spp.. On the other hand, the antibacterial activities of wood vinegars were determined by a paper disc method using the three gram positive and five gram negative bacteria. The unrefined wood vinegars showed a prominent effect on the suppression of bacteria growth at the concentration of 10%, while the suppression of bacteria growth was not observed in the refined wood vinegars. These results also suggest that the unrefined wood vinegars showed the higher antibacterial activities than the refined wood vinegars. The antibacterial activities of wood vinegars against gram positive bacteria were higher than those of wood vinegars against gram negative bacteria. From these results, it can be inferred that the wood vinegars has the strong antimicrobial activities and can be applied as the natural fungicides and food preservatives.