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Construction of A Bacteriocidal Yeast Producing Bacteriocin OR-7  

Lee, Ok-Hee (Department of Bioscience and Biotechnology, Silla University)
Jang, Min-Kyung (Department of Bioscience and Biotechnology, Silla University)
Lee, Dong-Geun (Department of Bioscience and Biotechnology, Silla University)
Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Silla University)
Ha, Jong-Myung (Department of Bioscience and Biotechnology, Silla University)
Ha, Bae-Jin (Department of Bioscience and Biotechnology, Silla University)
Ahn, Ik-Yong (Youngnam Flour Mills Co., LTD.)
Cho, Dong-In (Youngnam Flour Mills Co., LTD.)
Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Silla University)
Publication Information
Microbiology and Biotechnology Letters / v.36, no.2, 2008 , pp. 101-105 More about this Journal
Abstract
In order to obtain yeast cells producing a bacteriocin OR-7, the 180 bp polynucleotide corresponding to the OR-7 gene including codons for start and stop was chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Campylobacter jeuni, Escherichia coli and Pseudomonas aeruginosa. This result indicates that yeast cells producing OR-7 possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative C. jejuni, E. coli and P. aeruginosa cells. The recombinant yeast strain constructed in this study can be applied in the food preservative or animal feed.
Keywords
Bacteriocin; Bacillus subtilis; Campylobacter jejuni; Escherichia coli; OR-7; Pseudomonas aeruginosa; Saccharomyces cerevisiae; yeast;
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