• Title/Summary/Keyword: 유전체 분석

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Estimation of Genetic Parameters for Linear Type and Conformation Traits in Hanwoo Cows (한우 암소의 선형 및 외모심사형질에 대한 유전모수 추정)

  • Lee, Ki-Hwan;Koo, Yang-Mo;Kim, Jung-Il;Song, Chi-Eun;Jeoung, Yeoung-Ho;Noh, Jae-Kwang;Ha, Yu-Na;Cha, Dae-Hyeop;Son, Ji-Hyun;Park, Byong-Ho;Lee, Jae-Gu;Lee, Jung-Gyu;Lee, Ji-Hong;Do, Chang-Hee;Choi, Tae-Jeong
    • Journal of agriculture & life science
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    • v.51 no.6
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    • pp.89-105
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    • 2017
  • This study utilized 32,312 records of 17 linear type and 10 conformation traits(including final scores) of Hanwoo cows in the KAIA(Korea Animal Improvement Association) ('09~'10), with 60,556 animals in the pedigree file. Traits included stature, body length, strength, body depth, angularity, shank thickness, rump angle, rump length, pin bone width, thigh thickness, udder volume, teat length, teat placement, foot angle, hock angle, rear leg back view, body balance, breed characteristic, head development, forequarter quality, back line, rump, thigh development, udder development, leg line, and final score. Genetic and residual(co) variances were estimated using bi-trait pairwise analyses with EM-REML algorithm. Herd-year-classifier, year at classification, and calving stage were considered as fixed effects with classification months as a covariate. The heritability estimates ranged from 0.03(teat placement) to 0.42(body length). Rump length had the highest positive genetic correlation with pin bone width(0.96). Moreover, stature, body length, strength, and body depth had the highest positive genetic correlations with rump length, pin bone width, and thigh thickness(0.81-0.94). Stature, body length, strength, body depth, rump length, pin bone width, and thigh thickness traits also had high positive genetic correlations.

Transformation of Rice (Oryza sativa L.) with Phosphate Transporter cDNA from Tobacco (Nicotiana tabacum L.) (담배 인산수송자 유전자를 이용한 벼의 형질전환)

  • 유남희;윤성중
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.6
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    • pp.441-445
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    • 2000
  • In order to improve phosphate use efficiency of rice using phosphate transporter (PT), transgenic rice plants containing a tobacco PT gene were developed. Calli from Dongjinbyeo (Oryza sativa L.) were cocultured with A. tumefaciens LBA 4404 harboring PT gene. Multiplied calli were transferred to MS medium supplemented with 50 mg/L hygromycin, 500 mg/L carbenicillin, 2 mg/L kinetin, 0.1 mg/L NAA. After 2 weeks, hygromycin resistant shoots were obtained from the calli on the selection medium. The putative transgenic shoots were transferred to rooting MS medium supplemented with 250 mg/L cabenicillin. Plant regeneration rate from the calli was about 52%. Stable incorporation of the tobacco PT gene into rice genomic DNA was confirmed by PCR and Southern blot analysis.

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A cytogenetic study of Astragalus koraiensis Y. N. Lee (정선황기의 세포유전학적 연구)

  • Han, Sang Eun;Kim, Hyun-Hee;Heo, Kweon
    • Korean Journal of Plant Taxonomy
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    • v.43 no.2
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    • pp.139-145
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    • 2013
  • This study was carried out to determine the karyotype and chromosomal localizations of 45S and 5S rDNAs using FISH in Astragalus koraiensis. The somatic metaphase chromosome number of this species was 2n = 16 with basic chromosome number of x = 8. The karyotype of A. koraiensis was consisted of six pairs of median region chromosomes(chromosome 1, 3, 4, 5, 6, 8) and two pairs of submedian chromosomes(chromosome 2, 7). Based on the FISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 5. Whereas, two pair of 5S sites were detected on the short arm of chromosome 4 and centromeric region of chromosome 7, respectively. These are quite different patterns from A. membranaceus, A. membranaceus var. alpinus, and A. mongholicus. Although A. koraiensis is considered as Korean endemic species, therefore, it should be conducted out comparative FISH study with A. sikokianus and A. bhotanensis which are very similar to A. koraiensis morphologically.

Effects of Sus1, a component of TREX-2 complex, on growth and mRNA export in fission yeast (분열효모에서 TREX-2 복합체의 구성요소인 Sus1이 생장 및 mRNA 방출에 미치는 영향)

  • Bae, Soo Jeong;Yoon, Jin Ho
    • Korean Journal of Microbiology
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    • v.53 no.1
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    • pp.49-54
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    • 2017
  • Sus1 / ENY2 is a tiny conserved protein that is involved in chromatin remodeling and mRNA biogenesis. Sus1 is associated to two nuclear complexes, the transcriptional coactivator SAGA and the nuclear pore associated TREX2. In fission yeast, Schizosaccharomyces pombe, ortholog of Sus1 / ENY2 was identified from the genome database. Tetrad analysis showed that the S. pombe sus1 is not essential for growth. However, deletion of the sus1 gene caused cold-sensitive growth retardation with slight accumulation of $poly(A)^+$ RNA in the nucleus. And the Sus1-GFP protein is localized mainly in the nucleus. Yeast two-hybrid analysis and co-immunoprecipitation experiment showed that Sus1 interacts with Sac3, another subunit of TREX2 complex. These results suggest that S. pombe Sus1 is also involved in mRNA export from the nucleus as a component of TREX-2 complex.

Genetic variation and population structure of Asarum misandrum (Aristolochiaceae) in Korea (각시족도리풀(Asarum misandrum)의 유전적 다양성 및 집단 구조)

  • So, Soonku;Kim, Muyeol
    • Korean Journal of Plant Taxonomy
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    • v.43 no.3
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    • pp.181-187
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    • 2013
  • Genetic variation in Asaum misandrum, a woodland herb in Korea, was investigated based on allozyme analysis with starch gel electrophoresis. All of previously reported populations in Korea were sampled and seven loci from six enzymes were analyzed. Overall genetic variation of A. misandrum population showed considerably high levels of genetic variation within the species (A = 2.05, P = 71.4, $H_E$ = 0.294). A positive $F_{IS}$ value of A. misandrum indicated overall deficiency of heterozygotes, and a low $F_{ST}$ value (0.112) meant very little differentiation among populations. Factors contributing to the high levels of genetic diversity found within populations of A. misandrum include population maintenance via wide distribution range from Korea to Japan and primarily outcrossing breeding system. Although it showed moderate genetic diversity level, most habitats of the species were scattered and discontinuous. Besides, low numbers of individuals were found in the most habitats and individuals are collected frequently from the wild due to the unique shape of the flowers as well as the rarity of the species. Thus, there is a need to set up a reasonable conservation strategies including the maintenance mechanism of genetic diversity of A. misandrum.

Microwave Dielectric Properties and Far Infrared Spectrum of $(Pb_{1-x}Ca_x)(Fe_{0.5}Ta_{0.5})O_3$ Ceramics ($(Pb_{1-x}Ca_x)(Fe_{0.5}Ta_{0.5})O_3$ 세라믹스의 마이크로파 유전특성 및 Far Infrared Spectrum)

  • 박흥수;윤기현;김응수
    • Journal of the Korean Ceramic Society
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    • v.37 no.3
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    • pp.256-262
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    • 2000
  • The dielectric properties of complex perovskite ($Pb_{1-x}Ca_{x}$)($Fe_{0.5}Ta_{0.5}$)$O_{3}$ with >($0.5{\le}x{\ge}0.65$ were investigated at microwave frequencies. Dilectric constant decreased with increasing Ca content, and was directly proportional to the cube of average ionic ra야 of A-site. For the specimen of x=0.6 sintered at $1250^{\circ}C$ for 3 h in air, dielectric constant (k) of 63, QF of 11000 GHz, and the temperature coefficient of resonant frequency(TCF) of -14ppm/$^{\circ}C$ were obtained. As Ca content increased, TCF of the specimen negatively increased due to the reduction of the tolerance factor(t). Changes in intrinsic loss with varying Ca content was investigated by the infrared reflectivity spectra ranging 50 to 4000 $cm^{-1}$, which were calculated by the Kramers-Kronig analysis and classical oscillator model. The relative tendency of microwave dielectric properties of the ($Pb_{1-x}Ca_{x}$)($Fe_{0.5}Ta_{0.5}$)$O_{3}$ specimens calculated from the reflectivity data were in good agreement with the results by the post resonant method.

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Effects of Dielectric Layer Thickness and Electrode Structures on High Xe AC-PDP (High Xe AC PDP에서 전극구조와 유전체 두께에 따른 방전 특성 분석)

  • Heo, Jun;Kim, Yun-Gi;Kim, Dong-Hyun;Lee, Hea-June;Lee, Ho-Jun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.61 no.2
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    • pp.237-242
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    • 2012
  • In this paper, we investigated effects of ITO electrode geometry and dielectric layer thickness on the discharge Characteristic of AC PDP. As the dielectric thickness is decreased ($30{\sim}12{\mu}m$), firing and sustain voltage is decreased. Luminance and discharge power increase with decreasing dielectric layer thickness because of increasing capacitance between plasma and electrodes. Reactive power decreases with dielectric thickness due to reduced capacitance between sustain electrodes. For the high Xe test panel with small ITO electrode, luminous efficacy as well as luminance increase with decreasing dielectric layer thickness. This result suggest that high power density and small plasma volume is beneficial for high efficacy discharge.

Specimen of Storage and Analysis for Genomic Epidemiology (유전체 역학 연구를 위한 시료의 보관과 분석)

  • Lee, Kwan-Hee;Hong, Yun-Chul
    • Journal of Preventive Medicine and Public Health
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    • v.36 no.3
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    • pp.209-212
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    • 2003
  • Because of advances of technologies in the field of genmic epidemiology in the recent years, specimen collection, storage and analysis became an essential part of research methodologies. DNA is now being used in epidemiologic studies to evaluate genetic risk factors and specimens other than the fresh whole blood can De used for PCR. Therefore, All nucleated cells, such as buccal swabs and urine specimens, are suitable for DNA analysis. For an unlimited source of genomic DNA, EBV transformation of lymphocytes can be used for immortalization. However, the type of specimen collected in genomic epidemiologic studies will depend on the study where the epidemiologist play a leading role for the design. We also briefly described various finds of analysis for SNP that is an essential part of the genomic epidemiology.

Design and Implementation of a Band Pass Filter of Personal Communication System for information facilities (정보 설비용 통신장치의 대역통과 필터의 설계 및 구현)

  • 이헌용;김영달;윤중락;강명식
    • The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.10 no.3
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    • pp.47-54
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    • 1996
  • In this paper, the band pass filter was designed and constructed for personal communication services of information facilities. For the band pass filter design, after dielectric resonator structures were analyzed. J-inverter design theory and its simulation method were studied. The parameters obtained from design theory were practically applied to filter construction. The simulation results are well agreed with the measured result of constructed filter after fine tunings.

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