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http://dx.doi.org/10.11110/kjpt.2013.43.2.139

A cytogenetic study of Astragalus koraiensis Y. N. Lee  

Han, Sang Eun (Department of Applied Plant Science, Kangwon National University)
Kim, Hyun-Hee (Department of Life Science, Sahmyook University)
Heo, Kweon (Department of Applied Plant Science, Kangwon National University)
Publication Information
Korean Journal of Plant Taxonomy / v.43, no.2, 2013 , pp. 139-145 More about this Journal
Abstract
This study was carried out to determine the karyotype and chromosomal localizations of 45S and 5S rDNAs using FISH in Astragalus koraiensis. The somatic metaphase chromosome number of this species was 2n = 16 with basic chromosome number of x = 8. The karyotype of A. koraiensis was consisted of six pairs of median region chromosomes(chromosome 1, 3, 4, 5, 6, 8) and two pairs of submedian chromosomes(chromosome 2, 7). Based on the FISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 5. Whereas, two pair of 5S sites were detected on the short arm of chromosome 4 and centromeric region of chromosome 7, respectively. These are quite different patterns from A. membranaceus, A. membranaceus var. alpinus, and A. mongholicus. Although A. koraiensis is considered as Korean endemic species, therefore, it should be conducted out comparative FISH study with A. sikokianus and A. bhotanensis which are very similar to A. koraiensis morphologically.
Keywords
Astragalus L.; Astragalus koraiensis; karyotype analysis; FISH; rDNA;
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