• Title/Summary/Keyword: 유전체 분석

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Characterization of simple sequence repeats in the Pleurotus ostreatus cultivars, 'Heuktari' and 'Miso' (느타리버섯 품종 '흑타리'와 '미소'의 초위성체 특성구명)

  • Park, Bokyung;Ha, Byeong Seok;Kim, Min Keun;Lee, Byungjoo;Choi, Jong In;Ryu, Jae-San
    • Journal of Mushroom
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    • v.14 no.4
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    • pp.174-178
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    • 2016
  • Simple sequence repeats (SSR), also referred to "microsatellites" consist of tandemly repeated short DNA sequence motifs and have been applied in various marker-based studies. SSRs were isolated and characterized from 'Heuktari' and 'Miso', which are major oyster mushroom cultivars in Korea, by genome sequencing and bioinformatic analysis. The genome sizes of 'Heuktari' and 'Miso' were estimated to be 40.8 and 40.3 Mb, respectively, which are larger than those of other P. ostreatus species (PC9 and PC10) and smaller than those of P. eryngii (KNR2312P5). In total, 949 and 968 SSRs were found in the 'Heuktari' and 'Miso' genomes, respectively. Comparative analysis of five mushrooms including P. ostreatus var. florida (PC9 and PC15) and P. eryngii revealed that the number of SSRs in 'Heuktari' and 'Miso' were the highest among them. All mushrooms studied showed similar SSR distribution patterns. Tri-, hexa-, and octanucleotide motifs accounted for the top three fractions of all SSRs.

Genome sequence of Caballeronia sordidicola strain PAMC 26577 isolated from Cladonia sp., an Arctic lichen species (북극 지의류 Cladonia종에서 분리한 Caballeronia sordidicola균주 PAMC 26577의 유전체 서열 분석)

  • Yang, Jhung Ahn;Hong, Soon Gyu;Oh, Hyun-Myung
    • Korean Journal of Microbiology
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    • v.53 no.2
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    • pp.141-143
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    • 2017
  • Caballeronia sordidicola strain PAMC 26577 was isolated from Cladonia sp., a lichen collected from Svalbard Archipelago in the Arctic Ocean. Draft genomic sequences of PAMC 26577 were determined using Illumina and 182 contigs were submitted to GenBank and N50 value was 159,226. The genome of PAMC 26577 was comprised of 8,334,211 base pairs and %G+C content was 59.4. The genome included 8 ribosomal RNA genes and 51 tRNA genes as non-coding sequences. Protein-coding genes were 8,065 in number and they included central metabolism genes as well as butanol/butyrate biosynthesis, polyhydroxybutyrate metabolism, serine cycle methylotrophy genes, and glycogen metabolism. Membrane transporters were more than two-hundreds in number, but sugar phosphotransferase system and TRAP transporters were lacking. PAMC 26577 lacked CRISPR-associated sequences and proteins. No transposable elements were observed and there were only limited number of phage remnant regions with 11 phage-related genes.

Dielectric Thin Film Mirror Embedded Optical Fiber Couplers (유전체 박막 거울 내장형 광섬유 결합기)

  • 신종덕
    • Korean Journal of Optics and Photonics
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    • v.4 no.4
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    • pp.420-427
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    • 1993
  • Dielectric thin film mirrors are embedded in multimode and single-mode fibers by a fusion splicing technique. The fibers with $45{\circ}$ angled embedded mirrors serve as ultra-compact directional couplers with low excess optical loss of 0.2 dB for multimode and 0.5 dB for single mode at 1.3 ${\mu}m$ and excellent mechanical properties. The reflectance is wavelength dependent and strongly polarization depencient. Far-field scans of the reflected output power measured with a white-light source show a pattern which is almost circularly symmetric with aspect ratio of 1.09 at 5% of the peak power. The splitting ratio in a multimode coupler measured with a diode laser source is much less dependent on input coupling conditions than in conventional fused biconical-taper couplers, indicating that these couplers are less susceptible to modal noise occuring in optical fiber communication systems. Spectral properties of multilayer internal mirrors normal to the fiber axis have been investigated experimentally, and a matrix analysis has been used to explain the results.

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Genome sequence of Bifidobacterium dentium strain ATCC 15424 originally isolated from pleural fluid of an empyema patient (농흉 환자의 흉막액에서 분리된 Bifidobacterium dentium strain ATCC 15424의 유전체 염기서열 해독)

  • Moon, Ji-Hoi;Kim, Suegene;Yang, Seok Bin;Jang, Eun-Young;Shin, Seung-Yun;Lee, Jin-Yong;Lee, Jae-Hyung
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.280-282
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    • 2019
  • We present here a draft genome sequence of Bifidobacterium dentium strain ATCC 15424, originally isolated from pleural fluid of an empyema patient. The genome is 2,625,535 bp in length and has a GC content of 58.5%. The genome includes 2,154 protein-coding genes, 4 rRNAs, and 55 tRNAs. Unlike other B. dentium strains isolated from human dental caries, ATCC 15424 carries 247 strain-specific genes, including prophage remnants and type III/IV secretion system proteins, N-acetylmuramoyl-L-alanine amidase, and PRTRC system protein E. The sequence information will contribute to understanding of the natural variation of B. dentium as well as the genome diversity within the bacterial species.

Analysis of A-Sandwich Radome with Metamaterials Core (메타 물질 코어를 갖는 A-Sandwich 레이돔 전파 특성 해석)

  • Lee, Kyung-Won;Hong, Ic-Pyo;Park, Beom-Jun;Chung, Yeong-Chul;Yook, Jong-Gwan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.20 no.11
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    • pp.1161-1170
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    • 2009
  • In this paper, dielectric, drude model and ideal metamaterial are applied to core of A-Sandwich radome and each radome is analyzed using recursive method in Ku band. The main parameters of radome performance are insertion loss, insertion phase delay and depolarization. In case of ideal metamaterial, the radome using ideal metamaterial dose not generate depolarization because insertion loss, insertion phase delay and loss for incidence angle of wave do not happened. If circular polarization wave is incident on radome with meta material, transmitted wave also keeps circular polarization. In case of the dispersive metamaterial, the performance of radome using dispersive metamaterial is better than it of radome using dielectric in a part of frequency band. From these results, it is showed that metamaterial can be applied to various radome structure.

Complete genome sequence of Bacillus licheniformis 14ADL4 exhibiting resistance to clindamycin (Clindamycin 내성 Bacillus licheniformis 14ADL4의 유전체 염기서열)

  • Jeong, Do-Won;Lee, Byunghoon;Lee, Jong-Hoon
    • Korean Journal of Microbiology
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    • v.54 no.2
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    • pp.169-170
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    • 2018
  • Clindamycin resistant Bacillus licheniformis 14ADL4 was isolated from doenjang, a Korean high-salt-fermented soybean food. Strain 14ADL4 contains a single circular 4,332,232 bp chromosome with a G + C content of 45.86%. The complete genome of strain 14ADL4 includes lmrA and lmrB homologs may confer resistance to clindamycin.

An System for Efficient Management of Genomic Epidemiological Cohort Study Information from Koreans (한국인 유전체 역학 정보의 효율적 관리를 위한 정보 관리 시스템)

  • 양은주;안윤진;이지은;김규찬
    • Proceedings of the Korean Information Science Society Conference
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    • 2003.04a
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    • pp.635-637
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    • 2003
  • 본 논문에서는 경기도 안성과 안산 지역에 설치된 유전체역학센터에서 조사 지역에 거주하는 45세 이상 69세 이하의 성인을 대상으로 고혈압, 당뇨, 골다공증, 천식, 비만 등 총국민의료비용에서 큰 부분을 차지하는 4-5개의 중요 만성질환을 주요 분석 분야로 진행중에 있는 한국인 유전체 역학조사사업으로부터 산출되는 임상검사 및 역학정보를 입력.관리하는 시스템에 대해 소개하고 있다. 검진 대상자를 접수하는 접수자 및 정보 관리자는 본 시스템을 통해 검진 대상자의 개인식별 정보, 생활습관 정보 등과 같은 설문 정보와 임상 검사 정보를 입력 후 이에 대한 조회, 관리, 집계 현황 출력, 데이터 백업 등을 수행할 수 있다.

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An Analysis in Optimum Coupling Method of Cylindrical Dielectric Resonator Filter Designed by Non-decaying Mode Analysis (Non-decaying 모드 해석을 이용해서 설계한 원통형 유전체 공진기 여파기의 최적 결합 방법에 대한 분석)

  • Lee, Won-Hui;Park, Chang-Won;Kim, Tai-Shin;Hur, Jung;Lee, Sang-Young
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.38 no.7
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    • pp.14-21
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    • 2001
  • In this paper, we designed and fabricated C-band bandpass filter using dielectric resonators. From waveguide cutoff frequency which applied the region between adjacent dielectric resonators, the height of cavity is determined. The cavity's diameter is determined to the twice of dielectric resonator? diameter considering the conductor loss. The resonant frequency of the DR cavity is calculated with non decaying mode analysis. Conventionally, cylindrical dielectric resonator is analysed by Cohn's model which use the decaying mode in the region between dielectric resonator wall and circular cavity wall, which is an approximated method. The external quality factor, $Q_{ex}$ has found with simulation result using Ansoft's Maxwell simulation tool. The designed filter using dielectric resonators with dielectric constant of 45 has the passband center at 5.065GHz. The bandpass filter using dielectric resonators has about 1dB insertion loss, 20MHz bandwidth and more than 30dB attenuation at $f_0+15MHz$.

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대기압 유전격벽방전의 구동주파수 변화에 따른 전자가열 양상 변환에 대한 시뮬레이션 연구

  • Lee, Jeong-Yeol;Bae, Hyo-Won;Sim, Seung-Bo;Lee, Ho-Jun;Lee, Hae-Jun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.251.2-251.2
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    • 2014
  • 현재 의료 및 표면처리 분야에 많이 이용되고 있는 상온 대기압 플라즈마 중에서 유전체 격벽 방전(DBD) 장치는 비교적 간단한 구조를 가지며 sub-millimeters 사이즈에서도 매우 높은 플라즈마 밀도의 발생 및 유지가 가능하다. 그러나, 현재로선 이러한 Micro DBD의 특성을 실험적으로 분석하는 것은 장비의 한계가 있으므로, Particle-In-Cell 시뮬레이션을 이용하여 중요 플라즈마 변수들을 관찰하였다. 여기서 사용된 중요변수로는 13.56 MHz~600 MHz사이의 인가 주파수를 두었으며, 유전체 표면에서 양이온에 대한 이차전자 방출계수를 고려하였다, 또한 중성기체는 헬륨가스와 아르곤가스의 2가지 중성기체인 경우를 살펴보았다. 이러한 시뮬레이션을 통해 인가전압의 주기 대비 Ion transit time의 비율이 달라짐에 따라 플라즈마 쉬스의 특성변화와 함께 전자 에너지 확률함수(EEPF)의 특성이 달라진다는 것을 확인하고, 이러한 전자 가열 양상의 변화 원리에 대해서 분석하였다. 또한 주파수 비율 조정을 통한 전자온도, 파워, 효율 등을 조절하는 방법의 공학적 가치에 대해 의견을 제시하였다.

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Current status and prospects of genomics and bioinformatics in grapes (포도 유전체 연구현황 및 전망)

  • Hur, Youn Young;Jung, Sung Min;Yun, Hae Keun
    • Journal of Plant Biotechnology
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    • v.42 no.4
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    • pp.298-311
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    • 2015
  • Grape is one of the important fruit crops around the world, and exposed to disease and pests, and internal or environmental stresses in the vineyards. Breeding and cultivation of new varieties of high quality-grapes resistant to diseases and pests and tolerant to stresses are the most important steps in the grape production. However, conventional breeding has laborious and time-consuming procedures in maintaining and selecting seedlings in the fields. Development of molecular breeding technology through understanding of molecular mechanism of useful traits can be used as an alternative strategy to improve the efficiency of grape breeding program by cross hybridization in grape development programs. The completion of the grape genome sequencing project provided the way to discover the novel genes and to analyze their functions. Comparative genomics, transcriptomic analysis, and the genome-wide identification and analysis of useful genes as well as development of molecular marker for valuable traits could provide novel insights into fruit quality and the responses to diseases and stresses, and can be used as important information in molecular breeding programs for grape development.