Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Genome sequence of Caballeronia sordidicola strain PAMC 26577 isolated from Cladonia sp., an Arctic lichen species

북극 지의류 Cladonia종에서 분리한 Caballeronia sordidicola균주 PAMC 26577의 유전체 서열 분석

  • Yang, Jhung Ahn (Department of Marine-Bio Convergence Science, Specialized Graduate School Science & Technology Convergence, Pukyong National University) ;
  • Hong, Soon Gyu (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Oh, Hyun-Myung (Department of Marine-Bio Convergence Science, Specialized Graduate School Science & Technology Convergence, Pukyong National University)
  • 양정안 (부경대학교 과학기술융합전문대학원) ;
  • 홍순규 (극지연구소) ;
  • 오현명 (부경대학교 과학기술융합전문대학원)
  • Received : 2017.06.08
  • Accepted : 2017.06.08
  • Published : 2017.06.30
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Abstract

Caballeronia sordidicola strain PAMC 26577 was isolated from Cladonia sp., a lichen collected from Svalbard Archipelago in the Arctic Ocean. Draft genomic sequences of PAMC 26577 were determined using Illumina and 182 contigs were submitted to GenBank and N50 value was 159,226. The genome of PAMC 26577 was comprised of 8,334,211 base pairs and %G+C content was 59.4. The genome included 8 ribosomal RNA genes and 51 tRNA genes as non-coding sequences. Protein-coding genes were 8,065 in number and they included central metabolism genes as well as butanol/butyrate biosynthesis, polyhydroxybutyrate metabolism, serine cycle methylotrophy genes, and glycogen metabolism. Membrane transporters were more than two-hundreds in number, but sugar phosphotransferase system and TRAP transporters were lacking. PAMC 26577 lacked CRISPR-associated sequences and proteins. No transposable elements were observed and there were only limited number of phage remnant regions with 11 phage-related genes.

Caballeronia sordidicola 균주 PAMC 26577은 다산 기지 근처에서 채집된 지의류인 Cladonia 종에서 분리되었다. Illumina 방식으로 분석한 균주 PAMC 26577의 초안 유전체 서열은 182개의 콘티그로 이루어졌으며, N50값은 159,226 염기쌍 길이에 해당하였다. 초안 유전체로 총 8,334,211 염기쌍을 확인하였으며, 59.4% G+C 함량을 나타냈다. 유전체는 단백질을 코드하지 않는 8개의 rRNA 유전자와 51개의 tRNA 유전자를 포함하였다. 8,065개의 단백질 유전자는 기본 대사 과정뿐 아니라 부탄올/부티르산 생합성, 폴리하이드록시부티르산 대사, serine cycle methylotrophy 및 글라이코겐 대사 유전자들을 가지고 있었다. 2백개 이상의 막 전달 단백질은, 인산화 전달 시스템과 TRAP 전달시스템이 부재하였다. PAMC 26577은 CRISPR 관련 서열 및 단백질이 없었으며, 파아지 유전자의 감염흔적으로 인한 11개의 파아지 관련 유전자를 찾아낼 수 있었다.

Keywords

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