• Journal of Marine Life Science
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• v.6 no.2
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• pp.97-105
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• 2021

Tegillarca granosa, is one of the most important fishery resources throughout Asia. However, due to industrialization factories, marine environmental pollution, and global warming, the marine fishery production has drop sharply. In order to understand the genetic factors of the blood clam, which is a major fishery resource on the southern coast of Korea, the whole genome of blood clam was studied. The assembled genome of T. granosa was 915.4 Mb, and 19 chromosomes were identified. 25,134 genes were identified, and 22,745 genes were functionally annotated. As a result of performing gene gain and loss analysis between the blood clam genome and eight other types of shellfish, it was confirmed that 725 gene groups were expanded, and 479 gene groups were contracted. The homeobox gene cluster of blood clam showed a well-preserved genetic structure within lophotrochozoan ancestor. T. granosa genome showed high similarity between three hemoglobin genes with Scarpharca broughtonii. The blood clam genome will provide information for the genetic and physiological characteristics of blood clam adaptation, evolution, and the development of aquaculture industry.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.17-17
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• 2001

착상전 수정란 단계에서 형질전환 수정란의 선발은 형질전환동물의 효율을 증대시킬 수 있는 방법이다. 성공적인 형질전환동물의 생산을 위해서는 생산된 수정란의 mosaicism 빈도를 감소시켜 전체 할구에서의 유전자 발현을 유도하는 것이 최적일 것이다. 따라서 본 연구에서는 돼지의 웅성 생식세포를 이용한 형질전환동물의 생산에 있어서 다양한 정자세포 이용시 형질전환 수정란의 생산성 및 mosaicism 빈도를 조사하였다. 아울러 돼지 웅성생식세포내 GFP 유전자도입시 세포들의 생존율 및 원형정자세포분리 후 배양에 따른 형태적 변화를 관찰하였다. 돼지의 웅성 생식세포내 GFP 유전자 도입은 전기자극법 (1.3 ㎸/cm, 200 $\mu\textrm{s}$) 에 의하여 수행되었으며, 이 때 생존율은 60-70%이였다. 유전자가 도입된 전체 세포중 원형정자세포군의 분리는 유식세포분리기에 의하여 수행하였으며, 전체집단에 대한 분리군의 비율은 평균 16.2%이였다. 형질전환 수정란의 생산은 정자 (ICSI), 원형정자세포 (ROSI), 배양후 확장된 원형정자세포(ELSI)를 이용하였으며 각각의 난할율은 ICSI (82.9%), ROSI (59.1%), ELSI (62.1%)로 유의한 차이를 나타내었다. 그리고 8세포기까지의 배발달율은 각각 61.1, 40.9 및 48.6%이였으며, 상실배 및 포배기형성율은 각각 24.6, 18.1 및 32.4%이였다. 형광현미경하에서 GFP 단백질이 발현된 8세포기 수정란을 대상으로 각각의 할구를 primer extension pream-plification (PEP) PCR 방법으로 분석한 결과, ICSI 및 ROSI 실시후 대부분 (15/20, 9/10) 의 수정란은 3~4개의 할구에서만 GFP 유전자의 존재여부를 확인할 수 있었으며, 전체 할구에서 GFP 유전자가 모두 확인된 수정란은 없었다. 반면에 배양된 확장 원형정자세포를 이용하여 생산한 수정란의 경우, 4/10 (40%)에서 전체 할구내에 GFP 유전자의 존재를 확인할 수 있었다. 이러한 결과는 비록 배발달율 및 GFP 유전자 발현율에 있어서는 ELSI방법이 ICSI 등의 방법보다 현저히 낮았지만, mosaicsism 빈도가 낮아 바람직한 형질전환 수정란 생산에서는 오히려 유용한 방법이라고 사료된다. 또한 외래 유전자의 도입효율 면에서 후기 원형정자나 성숙정자보다 초기 원형정자세포에 외래유전자를 도입한 다음, 성숙시킨 확장원형 정자세포를 이용하는 방법이 보다 우수하다는 것을 시사하였다. 따라서 본 연구결과는 포유동물의 웅성 생식세포를 이용하여 nonmosaicisn을 나타내는 형질전환수정란을 생산하고 선발할 수 있는 일련의 기술적 과정을 정립하였다고 사료된다.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.1
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• pp.117-128
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• 2021

The skeletal muscle development of Hanwoo steer has been processed in the prenatal and postnatal periods. Bovine satellite cell located in perimysium of muscle tissues has differentially distributed in peripheral tissues. The study of postnatal development of satellite cells can help understand the genetic and functional regulation of meat characteristics. Factors affecting muscle size increase are related to the accumulation of DNA or synthesis of RNA proteins. In this study, we observed muscle development and differentiation after culturing bovine satellite cells derived from longissimus dorsi and semimembranosus regions of Hanwoo muscle tissue. In addition, RNA sequencing data were analyzed for differentially expressed genes (DEG) involved in intracellular muscle development and growth. The DEG of the two muscle tissues were compared according to 1day, 2day, 4day, and 7day. The overall gene expression level was confirmed by the heat map. Gene Ontology (GO) classification method was used to compare the expression level of gene groups affecting LD and SM development. The histology of GO was consistent with the time-cause change of LD and SM cell morphology. SM showed more active skeletal muscle development than LD. Even within the same time, SM expressed more genes than LD, thus synthesizing more muscle fibers

• Research in Plant Disease
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• v.22 no.1
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• pp.9-17
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• 2016

Nationwide survey for angular leaf spot (ALS) of strawberry caused by Xanthomonas fragariae, a quarantine disease in Korea, was performed in November 2012. In the survey, ALS was observed in eighty eight farmers' fields of Sukok, Jinju and Okjong, Hadong in Gyeongnam Province, and one field in Namwon of Jeollabuk Province. The infected field of Namwon closed immediately after the disease diagnosed ALS. In detailed survey of Sukok and Okjong areas during February 2012 to January 2015, ALS occurrence decreased from 45% farmer's fields on December 2012 to 5% on January 2015, and from 38% on November 2013 to 5% on January 2015, respectively. Phenotypic characteristics of the Korean strains were similar to those of the type strain of X. fragariae. A multilocus sequence analysis of Korean strains of X. fragariae was conducted using four genes; dnaK, fyuA, gyrB, and rpoD. All the Korean strains had the same sequences of the four genes. The concatenated sequences of the Korean strains shared 100% with that of the type strain of X. fragariae. All strawberry cultivars tested were susceptible to the strains of X. fragariae two weeks after inoculation. The inoculated sites were necrosis and expanded, which were rated 4 based on evaluation of inoculation site.

• Korean Journal of Construction Engineering and Management
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• v.16 no.5
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• pp.54-64
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• 2015

The modular construction has several advantages such as high quality of product, safe work condition and short construction duration. The manufacturing planning of modular construction should consider time frame of manufacturing, transport and erection process with limited resources (e.g., modular units, transporter and workers). The manufacturing planning of multi modular construction project manages the modular construction's characteristics and diversity of projects, as a type of modular unit, modular unit quantities, and date for delivery. However, current modular manufacturing planning techniques are weak in dealing with resource interactions and each project requirement in multi modular construction project environments. Inefficient allocation of resources during multi modular construction project may cause delays and cost overruns to construction operation. In this circumstance, this research suggest a manufacturing planning model for schedule optimization of multi project of modular construction, using genetic algorithm as one of the powerful method for schedule optimization with multiple constrained resources. Comparing to the result of the existed schedule of case study, setting optimized scheduling for multi project decrease the total factory producing schedule. By using proposed optimization tool, efficient allocation of resource and saving project time is expected.

• Radiation Oncology Journal
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• v.17 no.4
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• pp.299-306
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• 1999

Purpose : The human genetic disorder ataxia-telangiectasia (AT) is a multisystem disease characterized by extreme radiosensitivity. The recent identification of the gene mutated in AT, ATM, and the demonstration that it encodes a homologous domain of phosphatidylinositol 3-kinase (PI3-K), the catalytic subunit of an enzyme involved in transmitting signals from the cell surface to the nucleus, provide support for a role of this gene in signal transduction. Although ionizing radiation was known to induce c-fos transcription, nothing is known about how ATM or PKCI mediated signal transduction pathway modulates the c-fos gene transcription and gene expression. Here we have studied the effect of PKCI on radiation sensitivity and c-fos transcription in normal and AT cells. Materials and Methods: Normal (LM217) and AT (AT5BIVA) cells were transfected with PKCI expression plasmid and the overexpression and integration of PKCI was evaluated by northern blotting and polymerase chain reaction, respectively. 5 Gy of radiation was exposed to LM and AT cells transfected with PKCI expression plasmid and cells were harvested 48 hours after radiation and investigated apoptosis with TUNEL method. The c-fos transcription activity was studied by performing CAT assay of reporter gene after transfection of c-fos CAT plasmid into AT and LM cells. Results: Our results demonstrate for the first time a role of PKCI on the radiation sensitivity and c-fos expression in LM and AT cells. PKCI increased radiation induced apoptosis in LM cells but reduced apoptosis in AT cells. The basal c-fos transcription activity is 70 times lower in AT cells than that in LM cells. The c-fos transcription activity was repressed by overexpression of PKCI in LM cells but not in AT cells. After induction of c-fos by Ras protein, overexpression of PKCI repressed c-fos transcription in LM cells but not in AT cells Conclusion: Overexpression of PKCI increased radiation sensitivity and repressed c-fos transcription in LM cells but not in AT cells. The results may be a. reason of increased radiation sensitivity of AT cells. PKCI may be involved in an ionizing radiation induced signal transduction pathway responsible for radiation sensitivity and c-fos transcription. The data also provided evidence for novel transcriptional difference between LM and AT cells.

• Journal of Chest Surgery
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• v.35 no.5
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• pp.387-391
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• 2002

Background: Postoperative morbidity and mortality in destroyed lung are relatively high. We tried to identify the prognostic factors affecting postoperative morbidity and mortality in destroyed lung through a retrospective study. Material and method: The retrospective study was undertaken in 112 patients who had undergone pneumonectomy or pleuropneumonectomy for destroyed lung at Severance Hospital from 1970 to 2000. We analyzed the correlation between postoperative morbidity and mortality and etiology, duration of disease, preoperative FEV1, presence or absence of peroperative empyema, operation timing, the side of operation, duration of operation, and operation type. Result: There were 55 men and 57 women, aged 20 to 81 years (mean 44 years). Etiologic diseases were tuberculosis in 86 patients(76.8%) including tuberculos empyema in 20 and tuberculous bronchiectasis in 4, pyogenic empyema in 12(10,7%), bronchiectasis in 12(10.7%), and lung abscess in 2(1.8%). Postoperative morbidity were 25%(n=28) and postoperative mortality was 6%(n=7). The presence of preoperative empyema(p=0.016), pleuropneumonectomy(p=0.037) and preoperative FEV1 of less than 1.75 L(P=0.048) significantly increased the postoperative morbidity, If operation time was less than 300min, postoperative morbidity(p=0.002) and mortality(p=0.03) were significantly low. Conclusion: Postoperative morbidity and mortality in destroyed lung were acceptable. Postoperative morbidity and mortality were significantly low when operation time was less than 300 min. Preoperative existence of empyema, pleuropneumonectomy and preoperative FEV1 of less than 1.75 L significantly increased postoperative morbidity.