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http://dx.doi.org/10.23097/JPAF.2021.23(1):117

Transcriptomic Analysis of the Difference of Bovine Satellite Cell Between Longissimus dorsi and Semimembranosus on Hanwoo Muscle Tissues  

Kim, H.J. (Institute of Integrated Technology, CJ Blosson Park)
Kang, D.H. (Department of Beef Science, Korea National College of Agriculture & Fisheries)
Park, B.H. (Department of Beef Science, Korea National College of Agriculture & Fisheries)
Lee, W.Y. (Department of Beef Science, Korea National College of Agriculture & Fisheries)
Choi, J.H. (Department of Beef Science, Korea National College of Agriculture & Fisheries)
Chung, K.Y. (Department of Beef Science, Korea National College of Agriculture & Fisheries)
Publication Information
Journal of Practical Agriculture & Fisheries Research / v.23, no.1, 2021 , pp. 117-128 More about this Journal
Abstract
The skeletal muscle development of Hanwoo steer has been processed in the prenatal and postnatal periods. Bovine satellite cell located in perimysium of muscle tissues has differentially distributed in peripheral tissues. The study of postnatal development of satellite cells can help understand the genetic and functional regulation of meat characteristics. Factors affecting muscle size increase are related to the accumulation of DNA or synthesis of RNA proteins. In this study, we observed muscle development and differentiation after culturing bovine satellite cells derived from longissimus dorsi and semimembranosus regions of Hanwoo muscle tissue. In addition, RNA sequencing data were analyzed for differentially expressed genes (DEG) involved in intracellular muscle development and growth. The DEG of the two muscle tissues were compared according to 1day, 2day, 4day, and 7day. The overall gene expression level was confirmed by the heat map. Gene Ontology (GO) classification method was used to compare the expression level of gene groups affecting LD and SM development. The histology of GO was consistent with the time-cause change of LD and SM cell morphology. SM showed more active skeletal muscle development than LD. Even within the same time, SM expressed more genes than LD, thus synthesizing more muscle fibers
Keywords
Hanwoo; Muscle satellite cells; RNA sequencing; Myogenesis;
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