• Title/Summary/Keyword: 유전육종

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Construction of a Genetic Map using the SSR Markers Derived from "Wonwhang" of Pyrus pyrifolia (배 '원황'(Pyrus pyrifolia) 유전체 해독에 기반한 SSR 마커 개발 및 유전자 지도 작성)

  • Lee, Ji Yun;Seo, Mi-Suk;Won, So Youn;Lim, Kyoung Ah;Shin, Il Sheob;Choi, Dongsu;Kim, Jung Sun
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.434-441
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    • 2018

  • High-density genetic linkage mapping is critical for undertaking marker-assisted selection and confirming quantitative trait loci, as well as helping to build pseudomolecules of genomes. We constructed a genetic map using 94 $F_1$ populations generated from the interspecific cross between Korean cultivar "Wonwhang" (Pyrus pyrifolia, NCBI BioSample SAMN05196235) and European cultivar "Bartlett" (Pyrus communis). We designed a total of 24,267 SSR markers based on the genome sequences of "Wonwhang" for this. To select the markers that are linked to the traits important in pear breeding programs, SSR-containing genomic sequences were subjected to nucleotide sequence homology searches, which resulted in 510 SSR markers with high similarity to genes encoding proteins with putative functions such as transcription factors, resistance proteins, flowering time, and regulatory genes. Of these, 70 markers showed polymorphisms in parents and segregating populations and were used to construct a genetic linkage map, together with the unpublished 579 SNPs obtained from genotyping by sequencing analysis. The genetic linkage map covered 3,784.2 cM and the average distance between adjacent markers was 5.8 cM. Seventy SSR markers were distributed across 17 chromosomes with more than one locus.

  • https://doi.org/10.9787/KJBS.2018.50.4.434 인용 KSCI

Increased Antifungal Activity with Genetic Development of Antagonistic Pseudomonas stutzeri YPL-1 against Fusariym solani (식물근부균 Fusarium Solani에 길항하는 생물방제균 Pseudomonas stutzeri YPL-1의 유전공학적 개발)

  • 임호성;김상달
    • Microbiology and Biotechnology Letters
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    • v.18 no.4
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    • pp.437-441
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    • 1990

  • For the genetic development of more powerful antagonistic Pseudomom - YPL-1 as a biocontxol agent against soilborne plant pathogenic Fuaarium solani causing root rot of many important crops, mutants improving the productivity of chitinase were obtained by mutation with UV radiation or NTG treatment, P. stutzeri YPL-M26 (UV mutant) and P. stutzeri YPL-MI78 (NTG mutant) could improve the productivity of chitinase by 2.5 and 2.0 times, and its antifungal activity by 1.7 and 1.5 times, respectively. The antifungal mechanism of P. stutzeri YPL-M26 was caused by lysis of the fungal cell wall by hydrolytic enzymes such as chitinase. The antifungal activity of crude chitinase of P. stutzeri YPLM26 on the mycelial growth of F. solani was observed to be much higher than that of the original strain. The enzymes produced by P. stutzeri YPL-M26 were the same as the original strain in enzymatic properties such as optimal pH and temperature.

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