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http://dx.doi.org/10.9787/KJBS.2018.50.4.434

Construction of a Genetic Map using the SSR Markers Derived from "Wonwhang" of Pyrus pyrifolia  

Lee, Ji Yun (Genomics Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA)
Seo, Mi-Suk (Genomics Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA)
Won, So Youn (Genomics Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA)
Lim, Kyoung Ah (Genomics Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA)
Shin, Il Sheob (Postharvest Research Division, National Institute of Horticultural and Herbal Science, RDA)
Choi, Dongsu (Departments of Biology, Kunsan National University)
Kim, Jung Sun (Genomics Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA)
Publication Information
Korean Journal of Breeding Science / v.50, no.4, 2018 , pp. 434-441 More about this Journal
Abstract
High-density genetic linkage mapping is critical for undertaking marker-assisted selection and confirming quantitative trait loci, as well as helping to build pseudomolecules of genomes. We constructed a genetic map using 94 $F_1$ populations generated from the interspecific cross between Korean cultivar "Wonwhang" (Pyrus pyrifolia, NCBI BioSample SAMN05196235) and European cultivar "Bartlett" (Pyrus communis). We designed a total of 24,267 SSR markers based on the genome sequences of "Wonwhang" for this. To select the markers that are linked to the traits important in pear breeding programs, SSR-containing genomic sequences were subjected to nucleotide sequence homology searches, which resulted in 510 SSR markers with high similarity to genes encoding proteins with putative functions such as transcription factors, resistance proteins, flowering time, and regulatory genes. Of these, 70 markers showed polymorphisms in parents and segregating populations and were used to construct a genetic linkage map, together with the unpublished 579 SNPs obtained from genotyping by sequencing analysis. The genetic linkage map covered 3,784.2 cM and the average distance between adjacent markers was 5.8 cM. Seventy SSR markers were distributed across 17 chromosomes with more than one locus.
Keywords
Pear; Pyrus pyrifolia; SSR marker; Linkage map;
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