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Diversity and Phylogenetic Analysis of Culturable Marine Bacteria Isolated from Rhizosphere Soils of Suaeda japonica Makino in Suncheon Bay (순천만 칠면초의 근권으로부터 분리된 해양세균의 다양성 및 계통학적 분석)

  • You, Young-Hyun;Park, Jong Myong;Nam, Yoon-Jong;Kim, Hyun;Lee, Myung-Chul;Kim, Jong-Guk
    • Journal of Life Science
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    • v.25 no.2
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    • pp.189-196
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    • 2015
  • Bacterial diversity was studied in the rhizosphere of Suaeda japonica Makino, which is native to Suncheon Bay in South Korea. Soil samples from several sites were diluted serially, and pure isolation was performed by subculture using marine agar and tryptic soy agar media. Genomic DNA was extracted from 29 pure, isolated bacterial strains, after which their 16S rDNA sequences were amplified and analyzed. Phylogenetic analysis was performed to confirm their genetic relationship. The 29 bacterial strains were classified into five groups: phylum Firmicutes (44.8%), Gamma proteobacteria group (27.6%), Alpha proteobacteria group (10.3%), phylum Bacteriodetes (10.3%), and phylum Actinobacteria (6.8%). The most widely distributed genera were Bacillus (phylum Firmicutes), and Marinobacterium, Halomonas, and Vibrio (Gamma proteobacteria group). To confirm the bacterial diversity in rhizospheres of S. japonica, the diversity index was used at the genus level. The results show that bacterial diversity differed at each of the sampling sites. These 29 bacterial strains are thought to play a major role in material cycling at Suncheon Bay, in overcoming the sea/mud flat-specific environmental stress. Furthermore, some strains are assumed to be involved in a positive interaction with the halophyte S. japonica, as rhizospheric flora, with induction of growth promotion and plant defense mechanism.

Effect of Ovarian Changes according to Four Season for Reproduction of Jeju Crossbred Horses (Jeju crossbred에서 계절에 따른 난소주기 변화 연구)

  • Yu, Yeong-Ju;Park, Seol-Hwa;Shin, Sang-Min;Yang, Byoung-Chul;Seong, Pil-Nam;Woo, Jae-Hoon;Kim, Nam-Young;Son, Jun-Kyu
    • Journal of Embryo Transfer
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    • v.32 no.3
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    • pp.177-182
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    • 2017
  • This study was conducted to investigate the ovarian cycle changes of the mare according to the season. Twenty four Jeju crossbred horses(Thoroughbred ${\times}$ Jeju horse) raised in Subtropical Livestock Research Institute, National Institute of Animal Science, RDA were used to identify follicles and corpus luteum with ultrasonography once a week(May 2016~June 2017). Blood samples of experimental horses were collected twice a week for analysis of P4 hormone levels. The mares were considered to have resumed ovarian cyclicity on the day of ovulation if they followed by regular ovarian cycles. Only 13 cases(61.9%) of the total 21cases showed normal ovarian cycle, and 8 cases (38.1%) showed delayed ovarian cycle. Three cases(16.7%) in October, 5 cases(27.8%) in November and 5 cases(27.8%) in December(27.8%) ceased the heat and the remaining 5 cases(27.8%) showed that the estrus was maintained in winter. Horses that stopped estrus ceased the heat until March of next year, and 27.8% were continued the heat during non-breeding season. Eleven cases(61.1%) of 18 cases in April and 2 cases(11.1%) of 18 cases in May returned the estrus.

Effect of Glass Fiber-Reinforced Polymer (GFRP) Shear Connector's Shape on Inplane Shear Strength of Insulated Concrete Sandwich Panels (유리섬유복합체를 사용한 전단연결재 형상에 따른 중단열 벽체의 면내전단내력)

  • Jang, Seok-Joon;You, Young-Chan;Kim, Ho-Ryong;Yun, Hyun-Do
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.17 no.4
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    • pp.9-17
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    • 2013
  • This paper describes an experimental program to investigate the shear behavior of insulated concrete sandwich panels (CSPs) with different types of GFRP shear connector. The study included testing of 13 insulated CSP specimens with two types of surface conditions for extruded polystyrene (XPS) insulation and various shapes of shear connectors. All specimens were loaded in direct shear by means of push-out and were consist of three concrete panels, two insulation layer and four rows of GFRP shear connectors. Load-relative slip between concrete panel and insulation response of CSP specimens has been established through push-out shear test. Test results indicate that the surface condition of insulation has a significant effect on the bond strength between concrete panel and insulation. The specimen used XPS foam with 10mm deep slot shows higher bond strength than those used XPS foam with meshed surface. Corrugated GFRP shear connectors show equivalent strength to grid GFRP shear connectors. Cross-sectional area and embedded length of shear connector have a notable effect on overall response and inplane shear strength of the CSP specimens.

Isolation and Characterization of a Chitin Synthase Gene Fragments from Pleurotus sajor-caju (여름느타리의 Chitin synthase 유전자 단편분리 및 발현 특성 분석)

  • Jeong, Mi-Jeong;Park, Soo-Chul;Kim, Bum-Gi;Yoo, Young-Bok;Ryu, Jin-Chang
    • The Korean Journal of Mycology
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    • v.26 no.3 s.86
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    • pp.354-360
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    • 1998
  • We isolated three amplified DNA fragments from P. sajor-caju by Polmerase chain reaction (PCR) using the chithin synthase specific primers. Since the sequence analysis of the these fragments showed significant homology to the other known chitin synthase gene, we regarded these cloned fragments as PsCHS1, PsCHS2, and PsCHS3 according to their size. The PsCHS3, which showed the highest sequence homology (83% identity in amino acid level with ChsI of Rhizopus oligosporus in conserved region), was selected to see expression pattern of the corresponding gene. The result of RT-PCR using internal primer of the PsCHS3 fragment revealed that PsCHS3 gene was only expressed in cap and mycelium but not in stipe. In order to see whether the PsCHS3 gene was to be induced by wounding, the comparison of the mRNA level of this gene between wounded and unwounded mature cap showed at least two times induction of this gene by wounding treatment.

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Proximate and nutritional compositions of freeze-dried silkworm powder as edible insect resources (동결건조 누에분말의 일반 성분 및 영양 성분)

  • Kweon, HaeYong;Jo, You-Young;Kim, Hyunbok;Ju, Wan-Tack;Lee, Ji Hae
    • Journal of Sericultural and Entomological Science
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    • v.55 no.2
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    • pp.33-39
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    • 2019
  • Freeze-dried silkworm powder has been approved as a functional ingredients for blood glucose control since 2009. Proximate and nutrient compositions of silkworm powder was summerized as bellow. Silkworm powder contained 4.7% moisture, 57.7% crude protein, 9.5% crude fat, 5.4% crude fiber, and 9.3% ash. The larval protein contained 18 known amino acid including glutamic acid and aspartic acid. Fat is composed of 3:7 ratio of saturated fatty acid to unsaturated fatty acid including various essential fatty acids. It contained 34.7 linolenic acid, 24.4% oleic acid, 20.2% palmitic acid, 9.2% stearic acid, and 7.7% linoleic acid. Five minerals, K, P, Ca, Mg, S were abundant in the silkworm powder with other minerals such as Cu, Mn, Fe, and Zn.

Impact of Future Chinese Emissions on Ozone Air Quality and Human Health in Northeast Asia (동북아 지역에서 중국의 미래 배출량 변화가 오존농도와 보건에 미치는 영향)

  • Kim, Hyeon-Kook;Lyu, Youngsook;Woo, Jung-Hun;Hong, Sung-Chul;Kim, Deok-Rae;Seo, Jeonghyeon;Shin, Myunghwan;Kim, Sang-Kyun
    • Journal of Climate Change Research
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    • v.7 no.4
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    • pp.451-463
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    • 2016
  • We explore the impact of Chinese future air pollutant emissions on ozone air quality in Northeast Asia (NEA) and health in South-Korea using an assessment framework including ICAMS (The Integrated Climate and Air Quality Modeling System) and BenMAP (The Environmental Benefits Mapping and Analysis Program). The emissions data sets from the climate change scenarios, the Representative Concentration Pathways (RCPs) (emission scenarios, EMSO), are used to simulate ozone air quality in NEA in the current (1996~2005, 2000s), the near future (2016~2025, 2020s) and the distant future (2046~2055, 2050s). Furthermore, the simulated ozone changes in the 2050s are used to analyze ozone-related premature mortality and economic cost in South-Korea. While different EMSOs are applied to the China region, fixed EMSO are used for other country regions to isolate the impacts of the Chinese emissions. Predicted ozone changes in NEA are distinctively affected by large changes in NOx emission over most of China region. Comparing the 2020s with the 2000s situation, the largest increase in mean ozone concentrations in NEA is simulated under RCP 8.5 and similarly small increases are under other RCPs. In the 2050s in NEA, the largest increase in mean ozone concentrations is simulated under RCP 6.0 and leads to the occurrence of the highest premature mortalities and economic costs in South-Korea. Whereas, the largest decrease is simulated under RCP 4.5 leads to the highest avoided premature mortality numbers and economic costs. Our results suggest that continuous reduction of NOx emissions across the China region under an assertive climate change mitigation scenario like RCP 4.5 leads to improved future ozone air quality and health benefits in the NEA countries including South-Korea.

Seismic Performance Enhancement of Exposed Column-base Plate Weak-axis Connections for Small-Sized Steel Buildings (소규모 철골조건축물을 위한 약축방향 노출형 주각부의 내진성능 향상)

  • You, Young-Chan
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.23 no.5
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    • pp.1-12
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    • 2019
  • The purpose of this study is to enhance the seismic performance of exposed column-base plate weak-axis connections for small-sized steel buildings. According to the site inspection for the small-sized building construction, the arbitrary connection details in steel buildings have been applied at the job site, which is considered to be insufficient to guarantee structural safety and stability considering the increased seismic risk in Korea. Therefore, a series of test programs had been done to develop enhanced connection details in order to ensue the adequate seismic stability and safety of small-sized steel buildings. From the test results, It was found that the exposed column-base plate weak-axis connections commonly used in Korea shows very poor seismic behavior due to the "Rocking" phenomena caused by the residual plastic deformation of anchor bolts between anchor plate and concrete. A series of hysteretic tests for finding that solution were conducted to reduce the "Rocking" phenomena of the column-base plate connections, and local buckling of webs in H-column. Finally the enhanced stable seismic behavior was obtained by reinforcing at least 8 anchor bolts with good bonding strength and stiffeners to the webs in H-column.

Image-Based Automatic Bridge Component Classification Using Deep Learning (딥러닝을 활용한 이미지 기반 교량 구성요소 자동분류 네트워크 개발)

  • Cho, Munwon;Lee, Jae Hyuk;Ryu, Young-Moo;Park, Jeongjun;Yoon, Hyungchul
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.41 no.6
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    • pp.751-760
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    • 2021
  • Most bridges in Korea are over 20 years old, and many problems linked to their deterioration are being reported. The current practice for bridge inspection mainly depends on expert evaluation, which can be subjective. Recent studies have introduced data-driven methods using building information modeling, which can be more efficient and objective, but these methods require manual procedures that consume time and money. To overcome this, this study developed an image-based automaticbridge component classification network to reduce the time and cost required for converting the visual information of bridges to a digital model. The proposed method comprises two convolutional neural networks. The first network estimates the type of the bridge based on the superstructure, and the second network classifies the bridge components. In avalidation test, the proposed system automatically classified the components of 461 bridge images with 96.6 % of accuracy. The proposed approach is expected to contribute toward current bridge maintenance practice.

A Study on the Development of the Standard Manual for ETE (Emotion To Emotion) Therapy (오지상승위치료법의 표준매뉴얼 개발을 위한 타당화 연구)

  • Cheong, Moon Joo;Lee, Do-Eun;Kim, Jeesu;Kang, Sunghyun;Lyu, Yeoung Su;Jung, In Chul;Kang, Hyung Won
    • Journal of Oriental Neuropsychiatry
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    • v.33 no.3
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    • pp.227-239
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    • 2022
  • Objectives: The purpose of this study was to develop a standardization manual for Emotion To Emotion therapy. In this study, the processes and categories derived through literature search related to the Emotion To Emotion treatments, were revised and supplemented by the expert FGI (Focus Group Interview). Afterwards, the expert Delphi was conducted, to develop a standard manual for the disease types, purpose, and method of Emotion To Emotion therapy. Methods: In this study, literature analysis and expert Delphi, as a quantitative research method, were conducted, and the expert Focus Group Interview (FGI) was conducted as a qualitative study. The manual was completed by leading the consensus, on the standardization manual for Emotion To Emotion therapy. After that, a clinical expert Delphi was conducted to test the reliability as well as validity of the manual, through quantitative consensus on the manual of the Emotion To Emotion therapy. Results: First, as a result of literature studies, to date, studies related to Emotion To Emotion therapy have been qualitatively and quantitatively limited, as comparative literature related to clinical cases. Second, through expert FGI, the manual was structured with eight sub-factors for the indication diagnosis, six sub-factors for the implementation method, and 13 detailed factors. Third, through an expert Delphi, the consensus did the factor of indication, implementation methods, and implementation process, and developed a standardization manual for Emotion To Emotion therapy ver 1.0. Conclusions: Through literature analysis, expert FGI, and expert Delphi, the Emotion To Emotion therapy standardization manual ver 1.0 was completed, and will proceed with the revision and improvement report.

A Preliminary Survey Study on Standardization of the Core Seven Emotions Inventory-Short Form (CSEI-s) (핵심칠정척도 단축형의 표준화를 위한 예비적 설문 연구)

  • Jeesu Kim;Moon Joo Cheong;Do-Eun Lee;Yeoung Su Lyu;In-Chul Jung;Jeauk Kim;Hyung Won Kang
    • Journal of Oriental Neuropsychiatry
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    • v.34 no.3
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    • pp.125-139
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    • 2023
  • Objectives: This was a preliminary study to re-verify the reliability and validity of the Core Seven Emotions Inventory-Short Form (CSEI-s) and to establish standards for an objective scoring system such as T-scores and percentile for the normative group. Methods: Subjects were sampled through population-proportional regional allocation based on adults aged 20 or older and 65 or younger who voluntarily consented. Reliability was analyzed by performing an internal consistency (Cronbach's α) test and calculating means and standard deviations for each scale. The validity was analyzed between the CSEI-s and STAXI-K, SADS, COMOSWB, HADS, K-PANAS, Core emotions VAS. Results: The CSEI-s revealed notable gender discrepancies in aspects such as thought (思), fear (恐), and fright (驚), with women generally scoring higher than men. CSEI-s scores showed statistically significant differences in joy (喜) and thought (思) between those in their 20s and those in their 50s and 60s. As a result of the validity analysis, the seven-factor configuration was found to be statistically valid, and as a result of the correlation analysis with the coexistence scale, the correlation between each emotion and the seven emotions was found to be statistically significant. Cronbach's α for the CSEI-s was .891, showing statistically significant reliability. Conclusions: A preliminary study was conducted to standardize the shortened form of the Core Emotion Scale, and the reliability and validity were confirmed. A main survey of more people should be conducted in the future. Future results can contribute to revitalizing the development of oriental medicine evaluation tools and establishing an evidence base by standardizing the CSEI-s.