• Title/Summary/Keyword: 유식물체

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The Rhizome Growth and Shoot Induction Influenced by Ethylene in Cymbidium niveo-maginatum (옥화란(Cymbidium niveo-maginatum) Rhizome의 생장 및 유식물체 분화에 미치는 Ethylene의 영향)

  • 민병훈;정해준;이은경;황혜연;이영복
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.6
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    • pp.515-518
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    • 1998
  • The effect of ethylene on the proliferation of rhizomes and plant regeneration were investigated from rhizome segment culture of Cymbidium niveo-marginatum. Ethylene levels in the rhizome culture vessels were reached a maximum after 8 days of culture; total amount of ethylene evolution was much on the initiation of shoot induction than of rhizome proliferation. The treatment with ethephon on rhizomes was inhibited in the proliferation of rhizome and the growth of shoot length; however, the treatment was effective on shoot induction from rhizomes. Aminoethoxyvinylglycine(AVG) 1mg/L was effective on the proliferation of rhizomes and shoot induction from them; however, the proliferation of them was inhibited, and the growth of shoot length was significantly promoted at the concentration of 10mg/L AVG. The presence of $\textrm{AgNO}_{3}$ inhibited in the proliferation of rhizomes and shoot induction from them.

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Effect of Commercial Organic Medium Amended with Vermicast on the Growth of Rice Seedlings(Oryza sativa L.) -Amended with Vermicast of Fermented Pig Manure with Sawdust- (지렁이 분립을 첨가한 유기상토가 벼의 유식물체 생육에 미치는 영향 - 톱밥발효 돈분으로 생산한 분립의 첨가 -)

  • Lee, Ju-Sam;Kim, In-Soo
    • Korean Journal of Organic Agriculture
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    • v.16 no.3
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    • pp.299-308
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    • 2008
  • In this study, the effect of commercial organic growth medium amended with different ratios of vermicast on the growth of rice seedlings. The amended ratios of vermicast were 0% (control), 5%, 10%, 20% and 40%, respectively. A bioassay with two rice varieties (Chucheng and Black) was conducted to assesses the amendment effect of vermicast on the seedling growth in two potting methods. In potting treatment, the Chucheng variety with late maturity showed significantly higher values on growth parameters excepted root weight (RW) up to 20% and Black variety with early maturity up to 10% amended with vermicast, respectively. In floor layering treatment, all of the growth parameters significantly increased up to 5% amended with vermicast in both rice variety. The seedling growth of rice in floor layering treatment attained the highest values at lower amended ratios of vermicast than those of potting treated only, it may correspond with increased uptake of nutrient by elongated root grown under the layering amendment of vermicast. Vermicasts can be used as soil amendments or components of plant growth medium for seeding organic agriculture.

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The effect of sewage sludge compost amended soils on the growth of Orchardgrass seedlings (하수오니 첨가토양이 Orchardgrass 유식물체의 생육에 미치는 영향)

  • Lee, Ju Sam
    • Journal of the Korea Organic Resources Recycling Association
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    • v.2 no.2
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    • pp.77-88
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    • 1994
  • This experiment was carried out to investigate the effect of sewage sludge compost amended soils on the growth and accumulation patterns of heavy metals in plant parts of Orchardgrass seedlings, changes in physical properties and chemical composition, and heavy metal residue in soils. Mixture ratios of sewage sludge compost and soil(loam) were 100:0, 80:20, 60:40, 40:60, 20:80 and 0:100(control), respectively. The results obtained were as follows; 1. The physical properties and chemical compostion of soils were improved by increase in mixture ratios of sewage sludge compost. 2. The biological yield of Orchardgrass seedlings was increased with mixture ratios of sewage sludge compost. 3. The dry weight of shoot(SH) was increased with both of yield components(NT and WT) and biological yield of Orchardgrass seedlings. 4. The total nitrogen concentrations(TN) of plants was increased with quadratically up to the biological yield of 100% mixture ratio of sewage sludge compost. 5. Lead(Pb) concentration of soil in over the 60% mixture ratios of sewage sludge compost were in excess of limiting level(50ppm) of organic fertilizers.

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Effects of Low Dose Gamma Radiation on the Early Growth of Pepper and the Resistance to Subsequent High Dose of Radiation (저선량 방사선이 고추의 초기생육과 후속고선량 내성에 미치는 영향)

  • 김재성;백명화;김동희;이영근;이영복
    • Korean Journal of Environmental Biology
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    • v.19 no.1
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    • pp.71-77
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    • 2001
  • Pepper (Capsicum annuum L. cv. Jokwang and cv. Johong) seeds were irradiated with the doses of 0-50 Gy to investigate the effect of the low dose ${\gamma}$-radiation on the early growth and resistance to subsequent high dose of radiation. Following effect was observed in Johong cultivar but not in Jokwang cultivar Germination and early growth rate of Johong cultivar were noticeably increased at 4, 8 and 20 Gy irradiation group. Resistance to subsequent high dose of radiation of Johong cultivar increased at almost all of the low dose irradiation group. Especially it was highest at 4 Gy irradiation group. Plant groups of Johong cultivar showing the resistance to subsequent high dose of radiation, which had been pre-irradiated with 4 Gy and 8 Gy, were also higher in the carotenoid contents and enzyme activity than the plant group not irradiated previously.

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Effects of Growth Regulators on Shoot Differentiation and Bulblet Formation in Shoot-Tip and Bulb-Scale Cultures of Lilium longiflorum (백합 경단 및 인편배양으로부터 유식물체 분화 및 자구형성에 미치는 생장조절제의 영향)

  • 이은모;정해준;민병훈;이영복
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.2
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    • pp.83-87
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    • 1995
  • Regulation of organ differentiation by growth regulators was investigated through the shoot-tip and bulb-scale cultures of Lilium longiflorum (cv Georgia). When shoot tips were placed on MS medium supplemented with 0.1 mg/L NAA alone or 0.1 mg/L NAA and 0.1 mg/L BA, axillary shoots were proliferated. Root diffentiation and growth were stimulated on the basal medium. Although growth regulation did not seem to be necessary when bulb scales were used as explants for shoot differentiation, its differentiation was promoted vigorously by 0.2 mg/L NAA, but suppressed by BA. Bulblets were formed from bulb-scale-derived plantlets cultured on MS medium supplemented with 0.1 mg/L IBA. And more bulblets were formed from the plantlet in MS medium supplying 0.2 mg/L NAA with 6% than3% sucrose

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Systematic Propagation of High Quality Garlic (Allium sativum L.) Through Shoot Apical Meristem Culture II. Effects of Sucrose Concentration and Nitrogen Source on In Vitro formation of Bulblets (생장점배양에 의한 우량마늘 체계적 증식 II 기내 인경 비대에 미치는 질소 및 Sucrose의 영향)

  • Lee, Eun-Mo;Lee, Young-Bok
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.4
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    • pp.193-200
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    • 1994
  • The effects of sucrose concentration and nitrogen source on shoot growth and in vitro formation of garlic (Allium sativum L. cv Seosan) bulblet were investigated in order to systematize propagation of high quality garlic through a shoot apical meristem culture. Shoot differentiation was not affected by sucrose concentration and nitrogen source, but plantlets which contain medium of NH$_4$- N or NH$_4$ + NO$_3$ were vigorous and healthy in .appearance. Shoot growth was vigorous in changeing of nitrogen source. The best quality of in vitro bulblets was obtained in culture on the medium containing 8% sucrose and NH$_4$ - N, and the formation of bulblet was more effective when plantlets were subjected to cold treatment before use. NH$_4$-N was a major factor for shoot growth and bulblet development, but NO$_3$-N was not and suppressed $K^{+}$absorption. The level of ethylene production was not affected by different nitrogen sources, however this production was enhanced in medium containing a higher concentration of sucrose.e.

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Antioxidative Compounds in Extracts of Eleutherococcus senticosus Max. Plantlets (가시오갈피 유식물체 추출물의 항산화 활성물질)

  • Kim, Myong-Jo;Kwon, Yong-Soo;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.4
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    • pp.194-198
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    • 2005
  • Two antioxidative compounds were isolated from the methanolic extract of Eleutherococcus senticosus Max. plantlets and identified as chlorogenic acid and 1,4-di-o-caffeoyl-quinic acid on the basis of mass spectroscopy, $^1H-NMR$ and $^{13}C-NMR$ data. The DPPH free radical scavenging activities of chlorogenic acid $(RC_{50}\;:\;1.2\;{\mu}g)$ and 1,4-di-o-caffeoyl-quinic acid $(RC_{50}\;:\;0.4\;{\mu}g)$ were more effective than those of ${\alpha}-tocopherol\;(RC_{50}\;:\;12\;{\mu}g)$. Of them, 1,4-di-o-caffeoyl-quinic acid compound were isolated for the first time from this plant.

Callus growth and plant regeneration from hybrid embryo of L. longiflorum X L. elegans (L. longiflorum X L. elegans의 잡종 배로부터 캘러스의 증식 및 식물체 재분화)

  • Yoon, Eui-Soo;Kwon, Hye-Kyoung;Cho, Yi-Yun
    • Journal of Plant Biotechnology
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    • v.33 no.2
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    • pp.99-104
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    • 2006
  • This study was performed to investigate regeneration of plants differentiated from hybrid embryos between L. longifilorum Georgia and L. elegans Kakutanohikari. In addition, proliferation of callus and process of differentiation were investigated by histological observation. The germination of hybrid embryos was observed in 86 individuals from 48 slice cultures. Plant regeneration was effective on a medium supplemented with 1 mg/L HPh, and only callus proliferation was the highest in combination of 0.1 mg/L HPh and 1 mg/L BA. Also, plant regeneration was the most effective on a medium supplemented with 50 mg/L pyridoxine. We concluded that somatic embryos were formed from procambium of callus and proliferation of embryonic or proembryonic cells were stimulated with NAA from procambial cells.

Glutathione Content in Various Seedling Plants, Vegetables, and the Processed Foods (각종 유식물체, 채소 및 가공식품 중의 글루타치온 함량)

  • Kim, Ju-Sung;Shim, Ie-Sung;Kim, Myong-Jo
    • Korean Journal of Food Science and Technology
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    • v.41 no.5
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    • pp.592-596
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    • 2009
  • In this study, we investigated the levels of glutathione (GSH) and its oxidized form (GSSG) in more than 40 kinds of plant materials including seedling plants, grains, vegetables, and processed foods. The glutathione contents in the seedling plants were ranged from 0 to $120{\mu}mol/100g$. In addition, the different levels of glutathione were observed within the same family and between species. In the case of marketed grains and vegetables, azuki and kidney beans of leguminosae contained the high levels of glutathione, whereas glutathione was scarcely detected in the processed bean foods (bean paste, soybean sauce, etc.). Overall, a higher GSH content in food may contribute to a higher added value.

Plant Regeneration from Hairy Root of Rehmannia glutinosa Liboschitz Transformed by Agrobacterium rhizogenes (형질전환된 지황의 모상근으로부터 식물체의 재분화)

  • Hwang, Sung-Jin
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.1
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    • pp.31-35
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    • 2006
  • A protocol for plant regeneration from hairy root of Rehmannia glutinosa transformed by Agrobacterium rhizogenes ATCC15834 has been developed. Transgenic shoots were regenerated from hairy roots within 6 weeks after culture on the SH medium supplemented with 0.5 mg/l BA. Shoots were rooted on plant growth regulator free SH medium successfully. The transformed plants, which were regenerated from hairy roots, had thiner roots with extensive lateral branches, wrinkled leaves, shorter node, and grew faster compared with non-transformed plants. The biomass of the transformed plant was 1.28 g (F.W) per plant, significantly higher than the non-transformed plant (0.54 g F.W). The catalpol content in the transformed plant (0.56%) was also higher than that of the non-transformed plants (0.43%).