• Journal of Life Science
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• v.22 no.6
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• pp.772-777
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• 2012

The purpose of this study was to investigate the effects of black garlic supplementation and exercise on TBARS, HSP 70, and COX-2 expression after high-intensity exercise in rats. Twenty-four male Sprague-Dawley rats (4 weeks) were used in this study. Experimental groups were a control group (A, n=6), black garlic supplementation group (B, n=6), exercise group (C, n=6), and black garlic supplementation with exercise group (D, n=6). High-intensity exercise was performed by treadmill running (every 10 min speed increased gradually; 15~24 m/min, $0^{\circ}$ grade) and 2.86 g/kg black garlic for 4 weeks. The findings of this study were as follows: TBARS levels were significantly lower in the D group compared to the other group and B and C groups were significantly lower in TBARS levels than in the A group. HSP 70 expression was lower in the B group compared to the other groups. COX-2 expression has showed a similar expression in all groups. Therefore, it is considered that black garlic intake and exercise may reduce oxidative stress.

• Journal of Applied Biological Chemistry
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• v.53 no.1
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• pp.51-55
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• 2010

A lactic acid bacterial strain showing fast growth and high acid production in Korean pear puree was isolated from Kimchi. This strain was analyzed by API 50 CHL kit and 16S rRNA sequencing analysis and identified as Leuconostoc (Ln.) mesenteroides KACC 91495P. Korean pear puree was fermented using Ln. mesenteroides KACC 91495P strain at $30^{\circ}C$ for 18 h. The changes of pH, titratable acidity and viable cell number during fermentation were investigated. The pH and titratable acidity were reached to pH 3.86 and 1.09% after 18 h fermentation, respectively. The viable cell population of Ln. mesenteroides KACC 91495P was rapidly increased to $2.0{\times}10^9\;CFU/g$ during the 9 h of cultivation. The contents of lactic acid, acetic acid and malic acid were determined to be 0.213, 0.259, and 0.217% after 18 h fermentation, respectively. The content of polyphenolic compounds, known as antioxidants, in pear puree were enhanced by Ln. mesenteroides KACC 91495P cultivation. The level of total polyphenolic compounds was increased to around 140% of initial concentration. When the fermented pear puree was kept at $4^{\circ}C$, pH, titratable acidity and number of viable cells population were nearly maintained for 13 days.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 1992.04b
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• pp.581-591
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• 1992

최근 급속한 사무자동화와 함께 컴퓨터의 사용이 보편화되어 가지고 있다. 이에 따라 컴퓨터등 VDT에 매달려 일하는 사무직 근로자들이 시력장해, 두통, 어깨결림, 요통, 스트레스성 장애등 새로운 질병에 시달리고 있다. 이것은 "VDT증후군"(Visual Display Terminal Syndrome) 또는 "VDT병"이란 신종 직업병이다. 컴퓨터 수상기 화면을 계속해서 들여다보면 화면에서 나오는 자외선과 강한 빛으로 눈에 무리가 와 충혈되고 침침해지며 두통 증세가 나타난다. 이런 초기증상이 6개월이상 계속되면 시력이 떨어지고 만성피로를 느끼는가 하면, 때때로 독감과 비슷한 증상이 나타나며, 심하면 탈모현상까지 경험하게 된다. 요즘 시행되고 있는 초등 고교 컴퓨터교육의 의무화와 2천년대의 1가구 1단말기 설치를 목표로한 컴퓨터 영상단말기의 급속한 보급으로, 앞으로는 사무실뿐만 아니라 일반 가정에까지 컴퓨터 이용이 일상화될 전망이고 보면 VDT작업에 따른 건강장해 문제는 더이상 가볍게 볼 수 없는 문제이다. 이에 대해 선진국에서는 작업시간 규제, 회사가 종업원의 정기적인 시력검진 및 시력교정에 필요한 비용을 보조하도록 하는가하면 실작업환경 개선등 다각적인 대책을 마련하고 있다. 미국, 유럽, 일본등 선진국에서는 VDT작업 여성들의 유산 및 기형아 출산등 임신이상에 관한 실태보고서까지 나오고 있어 여성들에게 커다란 충격을 던져주고 있다. 그러나 우리나라에서는 VDT병 증상을 호소하는 근로자들의 직업병예방 및 대책에 큰 어려움을 겪고있다. 그러므로, VDT작업으로 인한 건강상의 문제, 작업환경 및 작업자세등을 조사하여 문제점과 예방대책을 살펴보고, 작업장을 인간공학적으로 설계하고 평가하여 사용자의 건강을 보호하고 생산성의 향상에 도움이 되고자 한다.생산성의 향상에 도움이 되고자 한다.Action Code를 계산처리부로 넘겨준다. 입력처리부에서는 Mouse와 Keyboard 어느것으로나 입력이 가능하도록 해준다. 출력처리부에서는 Action Code에 따라 계산처리부에서 계산된 결과를 화면에 보여주기 위한 각종 2D/3D Graphic Routine들이 포함되어있어 계산처리부에서 불러쓰도록 되어있다.에서는 전문가시스템 기법을 도입해서 사출금형 공정계획전문가의 지식과 경험을 획득하여 지식베이스를 구축하고, 전문가시스템 셀(shell)중 CLIPS를 이용하여 자동공정계획시스템인 Mold CAPP을 개발하였다.PP을 개발하였다.며 이와 상이한 결과도 보여주고 있다. 이상으로 볼 때 1) 가정하수의 질을 높이기 위하여 분뇨정화조 의 효율증대 2) 산업폐수의 공정별 폐수량의 조절 및 폐수성 상에 따른 총량규제에 대비한 효율증대 3) 하천의 오염부하와 자정능력 최대한 부여 4) 폐수처리를 위한 미생물제개발 및 오염지표 종 연구와 오염내성 생물의 연구등이 종합적 으로 수행되어야 한다. 5) 이상의 모든 조사와 연구결과를 객관적으로 표기할 수 있도록 하천의 이정표가 정해져야 하겠다.7%)에 비해 유의하게 낮았고 정상군과는 유의한 차이를 보이지 않았다.상고나성이 있었다. 혈중 호모시스테인 농도는 질병의 위험요인으로서 뿐 아니라 대사적으로 밀접하게 연관된 비타민 영양상태의 biomarker로서도 그 영향력이 크다고 할 수 있다. 따라서 성별에 다른 다양한 연령집단에서 건강한 일반인과 심혈관계 질환자 등을 대상으로 호모시스테인과 비타민 영양상태에 대한 연구가 체계적으로 이루어 져야 할 것이다.태를 보다 효율적으로 증진시킬 수 있는 대안이 마련되어져야 한다고 사료된다.$\ulcorner$순응$\lrcorner$의 범위를 벗어나지 않는다. 그렇기 때문에도

• Journal of Dairy Science and Biotechnology
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• v.37 no.4
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• pp.237-246
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• 2019

In this study, quality characteristics of yogurt supplemented with Angelica gigas Nakai leaf extract were examined. The pH of the yogurt ranged from 4.40 to 4.45 and the titratable acidity ranged from 0.96% to 0.98%. The viscosity tended to decrease with the addition of the Angelica gigas Nakai leaf extract, but did not affect stability during storage. In the range of 0.1% to 0.3%, lactic acid bacteria were present in the range of 1.9×10⁹ to 3.2×10⁹ CFU/mL. The decursin content in yogurt was quantitatively analyzed, depending on the addition of 0.1% to 0.3% of Angelica gigas Nakai leaf extract and was found to be 0.26 ㎍/g, 15.23 ㎍/g, and 23.57 ㎍/g respectively. Organic acid showed the highest generation of lactic acid. The antioxidant properties of yogurt were shown to increase with the addition of the Angelica gigas Nakai leaf extract. The sensory score of yogurt supplemented with 0.1% of the Angelica gigas Nakai leaf extract was highly valued, at a level similar to that of plain yogurt. Yogurt supplemented with 0.2% of the extract was rated above the normal score of 6.31 to 6.50. As shown by the results, the optimal concentration of Angelica gigas Nakai leaf extract for addition to yogurt was within 0.2%.

• Journal of Food Hygiene and Safety
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• v.19 no.3
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• pp.151-160
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• 2004

Enterococcus faecium MJ5-14 isolated from Meju produced a bacteriocin, which was antagonistic towards Listeria monocytogenes. Bacteriocin activity reached a maximum (640 BU/mL) after incubation for 12 hr, the early stationary phase, then dropped after the late stationary phase. Bacterocin of E. faecium MJ5-14 was extremely active against a wide range of Listeria species, including L. monocytogenes with sensitives up to about 640 BU/mL. In case of mixed culture with 105 CFU/mL L. monocytogenes and 105 CFU/mL E. faecium MJ5-14, the inhibitory effect against L. monocytogenes at $37^{\circ}C$ was higher than at $25^{\circ}C$. The mode of action was identified as bactericidal, because the addition of 100 BU/mL this bacteriocin to cell suspensions of L. monocytogenes KCTC 3569, led to a marked decrease in the number of viable cells. Further, when held in contact with bacteriocin of E. faecium MJ15-14 for 12 hr, L. monocytogenes KCTC 3569 displayed the disruption of the cells and an important efflux of the intracellular material.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.12 no.3
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• pp.155-160
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• 1979

Mechanical agitation in fermentation process enhances the fermentation activity of microbes by means of oxygen supply and frequent collision with substrate. However, the fermentation activity of microaerophilic bacteria is inhibited by the excess oxygen resulted from the mechanical agitation. On this account, a a strain of Lactobacillus bulgarius was cultured to know the inhibition effect of the mechanical agitation and determine the optimum conditions for tile process of lactic acid fermentation. The growth rate of L. bulgaricus and the consumption rate of tile substrate revealed an identical pattern in changes. The two rates were constant in the range of the modified Reynolds number of $1\times10^5\;to\;5\times10^5$ while they showed linear increase in the range of the modified Reynolds number of $1\times10^5\;to\;10\times10^5$. Under the conditions of the modified Reynolds number more than $10\times10^5$, the both rates decreased. At the modified Reynolds number of $10\times10^5$, the maximum specific growth rate and the saturation constant of L. bulgaricus were 0.58/hr and 6.74g/l, respectively.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.4
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• pp.365-371
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• 2014

To identify new active anti-wrinkle ingredients, this study investigated the anti-wrinkle effects of Schizandra chinensis Baillon fermented with Lactobacillus plantarum (SCF) by assessment of cytotoxicity of human dermal fibroblast, collagen biosynthesis, matrix metalloproteinase-I (MMP-1) inhibition and elastase inhibition. S. chinensis was fermented with L. rhamnosus for 1 day at $37^{\circ}C$. The cytotoxicity of SCF was evaluated by a cytopathic effect reduction method. Effects on collagen biosynthesis and matrix metalloproteinase-I (MMP-1) of SCF were evaluated by previous reported method using procollagen type-IC peptide EIA kit and Matrix Metalloproteinase-1 Biotrack activity Assay Kit, respectively. Elastase inhibition assay was conducted by reaction of enzyme and substrate using N-Suc-$(Ala)_3$-nitroanilide as the substrate. As the results, SCF didn't show cytotoxicity against human dermal fibroblast at concentration of $100{\mu}g/mL$. Also, SCF was increased collagen synthesis and showed inhibitory effect of MMP-1 (p < 0.05). In the elastase inhibition assay, the $IC_{50}$ of SCF was $36.4{\mu}g/mL$. Therefore, our results indicated that SCF possesses anti-wrinkle effects and can be used practically for anti-wrinkle care of skin.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.1
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• pp.69-78
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• 2017

Hwangryunhaedoktang (HHT) has been traditionally used as a preventive and therapeutic medicine to treat enervation and diverse chronic diseases. This study was designed to compare the antioxidant, anti-wrinkle and whitening effects of HHT extract and its fermented extract by Leuconostoc mesenteroides (FHHT). FHHT was prepared by inoculation of L. mesenteroides after the extraction procedure with 70% ethanol. HHT and FHHT was investigated via high-performance liquid chromatography (HPLC). Simultaneous qualitative analysis of two bioacitive components, berberine and palmatine. was achieved by comparing their retention times ($t_R$) and UV spectra with those of the standard components. Cell viability test results indicated that both HHT and FHHT were non-toxic. In DPPH radical scavenging ability, $SC_{50}$ values of the FHHT was $68.85{\mu}g/mL$, which is more effective than HHT. Moreover, FHHT showed higher expression in production of procollagen type I than HHT. In nontoxic concentration range, FHHT showed strong melanin production inhibitory effect in ${\alpha}-melanocyte$ stimulating hormone (${\alpha}-MSH$)-stimulated B16F10 cell ($IC_{50}=9.82{\mu}g/mL$). These results suggested that fermented extracts of hwangryunhaedoktang had considerable potential as a cosmetics ingredient with an antioxidant and anti-wrinkle and whitening effects.

• Korean Journal of Microbiology
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• v.41 no.2
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• pp.152-156
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• 2005

In the present study, lactic acid bacterium that has antibacterial activity against Helicobacter pylori was isolated from feces of newborn baby. The selection was based on the ability to inhibit the growth of H. pylori and to withstand harsh environmental conditions such as acidic pH and high bile concentration. By biochemical test and 16S rDNA sequencing, selected strain was turned out to be an Pediococcus acidilactici, therefore designated to P. acidilactici GMB7330. In order to investigate the inhibitory effects of P. acidilactici GMB7330 on the growth of H. pylori, we have tested in vitro studies such as cell viability and urease test. These results showed that antibacterial activity of P. acidilactici GMB7330 significantly decreased the viable cell count and urease activity of H. pylori. Antibacterial activity of P. acidilactici GMB7330 against H. pylori remained after pH adjustment to neutral, and the concentration of lactate produced from P. acidilactici GMB7330 was not enough to inhibit H. pylori. On the basis of the analysis by transmission electron microscope, it demonstrated that addition of P. acidilactici GMB7330 destroyed the cell structure of H. pylori. These results strongly suggested that P. acidilactici GMB7330 produce antibacterial substances to be able to inhibit the growth of H. pylori other than lactic acid.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.3
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• pp.213-218
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• 1987

The DNA damage mechanism by fish oil peroxidation was investigated through the model system of a DNA-mackerel lipid at $37^{\circ}C$. Mackerel lipid peroxidation products induced a great DNA damage with the increment of its concentration, and such DNA damage in all systems examined occurred below $100millieq{\cdot}/kg$ in POV (peroxide value) Singlet oxygen $(^1O_2)$ and superoxide anion${\cdot}O_2^-$ greatly participated in the DNA damage during peroxidation of mackerel lipid, while hydrogen peroxide$(H_2O_2)$ and hydroxyl radical $({\cdot}OH)$ did little show the DNA damage. From the results of the addition of several active oxygen scavengers to the DNA-lipid systems, singlet oxygen ana superoxide anion greatly affected to the increase of POV ana to the DNA damage by mackerel lipid peroxidation, respectively. It indicates that there was a close relationship between the effects of active oxygens in the mackerel lipid peroxidation and its DNA damage mechanism.