• Journal of Acupuncture Research
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• v.24 no.2
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• pp.1-14
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• 2007

목적 : 이 연구는 Vipera lebetina turanica의 사독약침파(蛇毒藥鍼波)(Snake venom toxin, SVT)이 in vitro에서 $NF-{\kappa}B$의 활성억제와 apoptosis 관련 단백질의 발현 조절을 통하여 세포자멸사(Apoptosis)를 유도하는지 in vivo에서 또한 전립선 암세포주인 PC-3 세포의 성장을 억제하는지 살펴보고자 하였다. 방법 : SVT를 처리한 후 PC-3의 성장억제를 관찰하기 위해 WST-1 assay, CCK-8 assay를 시행하였고，Apoptosis evaluation에는 DAPI, TUNEL staining assay를 시행하였으며，Apoptosis regulatory proteins의 변화 관찰에는 western blot analysis를 시행하였고，apoptosis와 연관된 $NF-{\kappa}B$의 활성 변화를 관찰하기 위해 EMSA시행하였으며，SVT의 핵내이동을 관찰하기 위해 Immunofluorescence Staining, Confocal immunocytochemistry를 시행하였으며，전립암세포의 종양형성에는 흉선을 제거한 쥐에 Tumorigenecity study를 시행하였다. 결과 : PC-3 세포에 SVT를 처리한 후，전립선 암세포의 성장，Apoptosis의 유발，Apoptosis관련 단백질의 발현，$NF-{\kappa}B$의 활성，SVT의 PC-3세포 핵내 이동여부 및 흉선제거 후 PC-3 세포를 이식한 쥐의 종양형성과정에 미치는 영향을 관찰하여 다음과 같은 결과를 얻었다. 1. PC-3 세포에서 SVT를 처리한 후 세포성장이 억제되고，세포자멸사가 유도되며，조절인자인 p53, caspase-3, -9는 증가되었고，Bcl-2는 감소되었다. 2. PC-3 세포에서 SVT를 처리한 후 $NF-{\kappa}B$의 활성이 유의하게 감소되었다. 3. DAPI로 염색된 상태에서 SVT가 PC-3 세포의 핵내로 이통되는 것이 관찰되었다. 4. 흉선제거 후 전립선 암세포주를 이식한 쥐에서 SVT를 피내로 주입한 결과 전립선암의 크기와 무게가 유의하게 감소하였다. 결론 : 이상의 결과는 SVT가 $NF-{\kappa}B$의 활성 억제를 통하여 인간 전립선암세포주인 PC-3의 세포자멸사를 유발함으로써 증식억제 효과가 있음을 입증한 것이며，이를 재확인한 생체 연구에서의 긍정적인 결과는 향후 SVT의 전립선암의 예방과 치료에 대한 효과적인 치료제 개발에 초석이 될 것으로 기대된다.

• Korean Journal of Food Science and Technology
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• v.46 no.4
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• pp.516-521
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• 2014

The mechanisms underlying the refractory effects of flutamide, a first-line oral anti-androgen drug, have not been entirely elucidated. In the present study, we investigated the mechanism of flutamide-induced hormone-refractory prostate cancer cell growth and its modulation by resveratrol, a phytoalexin present in grapes. Resveratrol significantly attenuated interleukin 6 (IL-6)-induced signal transducer and activator of transcription 3 (STAT3) transcriptional activity and dihydrotestosterone (DHT) or IL-6-induced prostate-specific antigen (PSA) transcriptional activity. Furthermore, compared to treatment with DHT or IL-6 alone, combination treatment of cells significantly increased PSA transcriptional activity, and resveratrol markedly diminished DHT plus IL-6-induced STAT3 and PSA transcriptional activities. Thus, the inhibitory effects of resveratrol on IL-6-, DHT-, and flutamide-induced hormone-refractory prostate cancer cell growth are partly mediated by the suppression of STAT3 reporter gene activity, suggesting that resveratrol represents a promising therapy for prostate cancer.

• The Journal of the Korea institute of electronic communication sciences
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• v.13 no.6
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• pp.1229-1234
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• 2018

In this paper, a fire prevention system based on a broadband powerline communication (PLC) system is implemented and a demonstration experiment is carried out to prevent from or promptly dealing with possible fires within the nacelle of a wind turbine generator system (WTGS). For this purpose, an inductive coupler having satisfactory attenuation characteristic in the frequency region for high-speed PLC is also manufactured. It is confirmed that the implemented system can monitor the environmental change inside the nacelle in real time by transmitting various information obtained by the sensors such as temperature, flame, and smoke sensor installed in the nacelle and thermal image recorded by a thermal camera to the ground control center through the PLC system. Therefore, it is, considered that the implemented system will significantly improve the reliability of the fire monitoring and prevention system of the WTGS in conjunction with the existing safety system.

• Journal of Radiation Protection and Research
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• v.21 no.3
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• pp.167-173
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• 1996

The calibration method for the detection efficiency of solid state track detector to alpha is presented, and the detection efficiency of CN-85 and LEXAN to alpha is measured by this method. The results are 97% and 57% in CN-85 and LEXAN, respectively. The reason for a better result in CN-85 in not only the high sensitivity but also the low energy dependency with comparing for LEXAN. The peak detection efficiency is 3MeV and 1.8MeV in CN-85 and LEXAN, respectively.

• Transactions of the Korean Society of Mechanical Engineers
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• v.17 no.6
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• pp.1478-1485
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• 1993

A shape optimization problem is formulated to determine the optimal position of heating lines in a compression molding die. The objective of the problem is that the cavity surface would be maintained by a prescribed uniform temperature. A boundary integral equation for the sensitivity of the temperature in terms of hole position is derived using the method of shape design sensitivity analysis. The boundary element method is employed to analyze the temperature and sensitivity field of the die. The sensitivity calculation algorithm is incorporated in an optimization routine. To demonstrate a numerical implementation, an example problem arising in thermal design of a compression molding die is dealt with, showing that the number of heating lines chosen for the die strongly affects the ultimate uniformity of the cavity surface temperature.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.4
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• pp.562-569
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• 2001

This paper presents the optimum design method for T-shaped microstrip-line-fed slot antennas on various substrates. Since the impedance bandwidth of the antenna depends highly on the vertical offset position and the length of horizontal strip conductor, we derived the formulas regarding these design parameters to get the maximum bandwidth. The formulas can compute the offset position and the length of horizontal strip conductor for any given substrate parameters. We compared the results obtained by using these design formulas and the one obtained by the simulation for each slot width, and it showed very good agreement.

• Proceedings of the KIPE Conference
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• 2008.06a
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• pp.326-328
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• 2008

본 논문의 목표는 실제 시스템과 가장 유사한 이중여자 유도발전기(Doubly-Fed Induction Generator)의 시뮬레이터를 구현하고 실험을 통해서 그것을 검증하는 것이다. 바람의 속도를 2D 룩업 테이블을 이용하여 데이터화 한 후 터빈 모델의 입력으로 인가한다. 터빈 모델의 파라미터는 실험용 이중여자 유도발전기의 것과 동일하게 설정하여 실제 시스템과 유사하게 하였다. 지금까지는 이중여자 유도발전기를 모사하기 위하여 매트랩(Matlab) 등을 이용하였지만 실제와 다소 다르다는 문제와 구현하기 힘들다는 문제를 가지고 있었다. 하지만 본 논문에서는 심플로러(Simplorer)를 이용하여 쉽고 정확한 시뮬레이터를 구현하였다. 터빈과 이중여자 유도발전기는 기어를 이용하여 직결함으로써 실제 시스템과 매우 가깝게 모사하였으며 이중여자 유도발전기의 회전자를 여자시키기 위해 이산 캐리어 변조 방법을 이용하여 매트릭스 컨버터를 구동하였다. 그리고 실험을 통해서 시뮬레이터의 결과와 실험 결과의 비교 및 검증을 한다. 궁극적으로 어떤 지역의 바람의 품질과 발전량 등 중요한 정보를 얻기 위해 실제 크기의 발전기를 설치하지 않고 본 논문에서 개발한 시뮬레이터를 이용하여 쉽게 그 정보들을 얻을 수 있다.

• Korean Journal of Food Science and Technology
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• v.44 no.6
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• pp.759-762
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• 2012

Egg allergies have been reported as one of the most prevalent food hypersensitivities in the pediatric population. One of the major egg allergens is ovalbumin (OVA), which is the major protein in the egg whites. Phenethyl isothiocyanate (PEIC) from cruciferous vegetables has an effect on anti-inflammatory therapy. In the present report, we show that PEIC inhibits the nuclear factor-${\kappa}B$ (NF-${\kappa}B$) activation induced by OVA. PEIC also inhibits the OVA-induced inducible nitric oxide synthase (iNOS) expression and nitrite production. However, PEIC did not suppress the cyclooxygenase-2 (COX-2) expression induced by OVA. These results suggest that PEIC has the specific mechanism for anti-inflammatory responses and efficient anti-allergic activities.

• The Journal of Natural Sciences
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• v.12 no.1
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• pp.41-48
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• 2002

Atomic coherences can be induced via atom-light interaction and can exhibit new interesting phenomena. Electromagnetically-induced-absorption (EIA) is one of such phenomena where the absorption of probe beam is increased due to the presence of strong coupling beam. EIA can be observed in multiple-V type atomic systems. This paper present a method to study EIA when the frequencies of probe beam and coupling beam are both near resonant to the same transition line. Time-varying interaction Hamiltonian was introduced and density matrix equations were solved and the amplitude of oscillations was used to calculate the EIA.

• Journal of Mushroom
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• v.2 no.4
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• pp.184-191
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• 2004

Mutual growth limitation was observation when the two antagonistic fungi was come in contact with each other. Brown line was formed 2day after contact with Trichoderma spp., and then, green spores formed overnight. The laccase activity of L. edodes was stimulated when this fungus wsa co-incubated with Trichoderma spp. for a few days in liquid media. In sawdust-rice bran nixtures, outstanding broun line developed when the two antagonistic fungi co-cultured. The pH of the substrates changed from 5.5 to 4.5 after overgrowth, suggesting a difference in the degradation ability and the preference of the two fungi for the lignocellulose material.