• Korean Journal of Microbiology
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• v.36 no.1
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• pp.52-57
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• 2000

A method for quantitative and qualitative analysis of geosmin, odorant produced by several actinomycetes and cyanobacteria, was established and optimized. The effects of environmental conditions on the growth of Phormidium sp. NIVA-CYA7 were examined and the production and release of geosmin by the species was analyzed by using the purge and trap-gas chromatographic technique. One of the major advantages of the technique established in the present study is that the preparation of sample is simpler and purge time is shorter. Under the culture conditions (pH 7.9, $20^{\circ}C$, 120-140 $\mu$E/$m^2$/s and Z8 medium), Phormidium showed growth characteristics with a lag phase for 8 days and an exponential phase for 14 days followed by a stationary phase. Reduction of inorganic nitrogen concentrations in the culture medium from 250 to 100 or 25 $\mu$M brought no significant effect on the cell growth. However, the cell growth was significantly inhibited with decreasing concentrations of inorganic phosphorus from 25 to 10 or 2.5 $\mu$M. When the inorganic phosphorus concentration in the medium was lowered from 25 to 10 $\mu$M, the levels of geosmin in the organism expressed as percentages per unit TOC and chlorophyII-$\alpha$ increased by 35% and 68%, respectively. When the initial pH of the medium was 9.4, geosmin content was 0.0824 $\mu$g/mg C, which was 2-fold higher than that at pH 7.9 Consequently, the level of geosmin in Phormidium was found to vary with growth phases of the culture, external inorganic phosphorus concentration and external pH, while the release of geosmin was not significantly affected by the factors.

• Analytical Science and Technology
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• v.16 no.6
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• pp.483-487
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• 2003

Amitrole is well-known as a non-selective herbicide and it is able to cause contamination of driking water as well as pollution of ground water and surface water. However, it is difficult to extract from water because it has a high solubility for water whereas a low solubility for general organic solvents. This method is described for the determination of amitrole in water samples by GC/MS. After evaporation of 10 mL water sample by a vacuum evaporator, amitrole was derivatized with isobutyl chloroformate (iso-BCF) on room temperature for 15~20 min. As a result, the sensitivity for GCfMS was improved as N-isobutoxycarbonyl amitrole derivative was formed. The linearity of the calibration curve showed good as 0.997. The recoveries were obtained more than 94.9% and relative standard deviations were less than 2.8% at $1.0{\mu}g/L$, $10.0{\mu}g/L$ and $100.0{\mu}g/L$. The limit of detection showed $0.1{\mu}g/L$ with a signal-to-noise ratio (S/N) of 3.

• Analytical Science and Technology
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• v.17 no.3
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• pp.263-270
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• 2004

A method is described for the analysis of short-chain alkylphenol ethoxylates (APEOs), 4-octylphenol-di-ethoxylate (OP2EO) and 4-nonylphenol-di-ethoxylate (NP2EO), in drinking water or wastewater using reversed phase high-performance liquid chromatography with electrospray ionization mass spectrometry. The solvent system was water and methanol containing $10{\mu}M$ trifluoroacetic acid as an ionization solvent. We acidified 1 L of water samples to less than pH 2 with concentrated $H_2SO_4$ and loaded onto Sep-Pak $C_{18}$, and eluted with acetone. The calibration of OP2EO and NP2EO was performed for the concentration range from 20 to 500 ng/L and the correlation coefficients were 0.999 and 0.990, respectively. The limits of detection were 20 ng/L (OP2EO) and 50 ng/L (NP2EO) at a signal-to-noise ratio of 3. Accuracy and precision of this analytical method were 85.8 ~ 122.1% and 8.2 ~ 18.8%, respectively. The proposed method allowed a sensitive and rapid detection of OP2EO and NP2EO and it could be applied for monitoring of APEOs from environmental samples.

• Economic and Environmental Geology
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• v.42 no.4
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• pp.335-342
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• 2009

The effects of bacterial lysis on the rate of quartz dissolution were investigated under pH 7 condition using Bacillus subtilis cells which were either irradiated or non-irradiated with gamma ray. The amount of dissolved organic carbon (DOC) which resulted from bacterial lysis increased in slurries of quartz and bacteria mixture over experimental period. Lysis of non-irradiated bacteria led to the elevated concentration of dissolved silicon when compared with abiotic control. Concomitant increase in the amounts of DOC and dissolved silicon over time indicated that lixiviation of silicon from quartz was due to bacterial lysis. Higher amounts of DOC and dissolved silicon were present in the irradiated bacterial slurries than those of non-irradiated bacteria. The enhancement of quartz dissolution in the irradiated bacterial slurries was likely attributed to disruption of organic molecules in the bacterial cells by gamma ray and formation of effective ligands for quartz dissolution. The results suggest that the effects of bacterial lysis on mineral weathering rate should be considered for prediction of time for released radionuclides to migrate to surface biosphere in high level radioactive waste disposal site.

• Economic and Environmental Geology
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• v.50 no.1
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• pp.15-26
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• 2017

Advanced argillic, argillic, and phyllic zones are the most important alteration patterns to predict the hidden ore body during exploration of hydrothermal deposits. We examined the quantitative relationship between the spectral absorption characteristics and the mineral content of the synthetic mixtures such as alunite-kaolinite and illite-kaolinite using short wavelength infrared (SWIR) spectroscopy. In the alunite-kaolinite mixtures, the spectral absorption characteristics of the alunite was highly correlated with the Hull quotient reflectance(0.99) and the kaolinite had the highest correlation with the Gaussian peak(0.92). Illite-kaolinite mixtures are essential for Gaussian deconvolution because of the overlap of absorption region. Illite and kaolinite mixtures indicate the high correlation of 0.93 and 0.98, respectively. The error ranges in the alunite-kaolinite(8%) and illite-kaolinite mixtures(5%) derived from SWIR were smaller than the ones(29% and 26%) obtained from X-ray diffraction(Rietveld) analysis. These results show that SWIR spectroscopic analysis is more reliable than XRD Rietveld analysis in terms of quantification of allowed minerals.

• Journal of Food Hygiene and Safety
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• v.22 no.2
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• pp.110-115
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• 2007

This test was performed to evaluate the skin irritation and sensitization of Creocomplex, disinfectant, Containing 10% 4-chloro-m-cresol, 10% didecyl dimethyl ammonium chloride, and 10% glutaraldehyde. In primary skin irritation test, rabbits were dermally treated with Creocomplex for 24 hrs. The disinfectant did not induce any adverse reactions such as erythema and edema on intact skin sites, but on abraded skin sites, some rabbits showed very slight erythema and edema 24hr after topical application. So, the disinfectant was classified as a practically non-irrifating material based on the score 0.13 of primary irritation index. In the skin sensitization test, guinea pigs were sensitized with intradermal injection of 0.1ml Creocomplex for 24 hr. After 1 week, Creocomplex was treated on the site of injection, and challenged 2 weeks later. Creocomplex did not induce any allergic reactions. Therefore, 10% Creocomplex was graded as a weak material from 0 in both sensitization score· and rate. From results of the present study, it is suggested that 10% Creocomplex does not cause contact irritation and sensitization.

• 한국공간정보시스템학회:학술대회논문집
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• 2004.12a
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• pp.139-143
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• 2004

최근 고 해상도 위성영상정보의 민간 활용에 대한 수요가 증가하면서 기존의 공간 정보를 다루는 여러 응용분야에서 이에 관련된 많은 연구를 하고 있다. 도시교통 환경 분석을 위하여 위성영상정보를 처리하는 과정에 있어서 도로, 건물, 기타 선 구조와 같은 지형지물을 분석하는 과정은 사용자에 따라 주관적일 수 있다. 이러한 배경에서 수학적 그레이 레벨 모폴로지는 하나의 효과적인 접근으로 간주된다. 본 연구에서 지형지물 추출을 위해 윈도우 운영체제에서 실행되는 실질적인 응용 프로그램을 구현하였다. 이 프로그램에서 주요한 지형지물은 그레이 레벨 영상을 이용하여 개방(opening), 폐쇄(closing), 침식(erosion), 팽창(dilation)의 순차적 처리를 통하여 자동적으로 추출된다. 결과적으로, GDPA, 허프 변환 또는 다른 알고리듬들과 비교시 하나의 이점이 된다. 모폴로지 처리와 같이 본 프로그램은 그레이 레벨 값의 범위에 기반하여 지형지물을 추출을 위한 density slicing 기능 또는 주어진 경계치 보다 작은 화소 군집을 제거하는 처리인 'sieve filtering'을 제공한다. 이러한 기능들은 형태학적으로 처리된 결과를 증대하고 지형지물 종류들을 분리하는데 유용하다. 또한 배경의 제거, 잡음 탐지, 도시 환경 원격 탐사에서의 지형지물 특성화에 기여한다. 본 프로그램을 이용하는데 있어서 IKONOS 위성영상을 이용하여 시험 구현하였다. 결과, 다중 경계치 또는 steve filtering을 이용한 그레이 레벨 모폴로지 처리는 복잡한 지형지물과 많은 데이터로 구성된 고해상도 영상 내의 주어진 대상에서 자동적인 처리와 사용자 정의 sieve filtering으로 인한 효과적인 지형지물 추출 방법으로 간주 된다. 시안을 작성 표준화를 위한 첫 단계 시도를 소개하였다.분석 결과는 문장, 그림 및 도표, 장 끝의 질문, 학생의 학습 활동 수 등이 $0.4{\sim}1.5$ 사이의 값으로 학생 참여를 적절히 유도하는 발견 지향적 인 것으로 조사되었다. 그러나 장의 요약은 본문 내용을 반복하는 내용으로 구성되었다. 이와 같이 공통과학 과목은 새로운 현대 사회에 부응하는 교과 목표와 체계를 지향하고 있지만 아직도 통합과학으로서의 내용과 체계를 완전히 갖추고 있지 못할 뿐만 아니라 현재 사용되고 있는 7종의 교과서가 교육 목표를 충분히 반영하지 못하고 있다. 따라서 교사의 역할이 더욱더 중요하게 되었다.괴리가 작아진다. 이 결과에 따르면 위탁증거금의 징수는 그 제도의 취지에 부합되고 있다. 다만 제도운용상의 이유이거나 혹은 우리나라 주식시장의 투자자들이 비합리적인 투자형태를 보임에 따라 그 정책적 효과는 때로 역기능적인 결과로 초래하였다. 그럼에도 불구하고 이 연구결과를 통하여 최소한 주식시장(株式市場)에서 위탁증거금제도는 그 제도적 의의가 여전히 있다는 사실이 확인되었다. 또한 우리나라 주식시장에서 통상 과열투기 행위가 빈번히 일어나 주식시장을 교란시킴으로써 건전한 투자풍토조성에 저해된다는 저간의 우려가 매우 커왔으나 표본 기간동안에 대하여 실증분석을 한 결과 주식시장 전체적으로 볼 때 주가변동율(株價變動率), 특히 초과주가변동율(超過株價變動率)에 미치는 영향이 그다지 심각한 정도는 아니었으며 오히려 우리나라의 주식시장은 미국시장에 비해 주가가 비교적 안정적인 수준을 유지해 왔다고 볼 수 있다.36.4%)와 외식을 선호(29.1%)${\lrcorner}$ 하기 때문에 패스트푸드를 이용하게 된 것으로 응답 하였으며,

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.5
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• pp.13-22
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• 2018

In this study, a governing differential equation for the bending problem of orthotropic rectangular plate is drived. It's exact solution for various boundary conditions is presented. This solution follows traditional method like Navier's solution or Levy's solution that transforms the governing differential equation into an algebraic equation by using trigonometric series. To obtain a solution by Levy's method, it is required that two opposite edges of the plate be simply supported. And the boundary conditions, for which the Navier's method is applicable, are simply supported edge at all edges. In this study, it overcomes the limitations of the previous Navier's and Levy's methods.This solution is applicable for any combination of boundary conditions with simply supported edge and clamped edge in x, y direction. The plate could be subjected to uniform, partially uniform, and line loads. The advantage of the solution is that it is the exact solution as well as it overcomes the limitations of the previous Navier's and Levy's methods. Calculations are presented for orthotropic plates with nonsymmetric boundary conditions. Comparisons between the result of this paper and the result of Navier, Levy and Szilard solutions are made for the isotropic plates. The deflections were in excellent agreement.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.8
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• pp.5088-5094
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• 2014

Hovenia dulcis extract (HDE) has positive effects on alcohol degradation, recovery of liver damage and antioxidant activities. This study examined whether HDE exerts an ameliorative effect on inflammatory orifacial pain in an animal algesic model with formalin. The animals (rats) were divided into four groups: group I (control), group II (right facial subcutaneous injection of 5% formalin, inflammatory orifacial pain group), group III (5% formalin + distilled water administration), and group IV injection (5% formalin + 4.5 ml/kg of HDE), respectively. The scores from the scratch and effleurage tests were applied to evaluate the differences between three groups. The expression of p38 MAPK, iNOS and Nrf2 in the brain and medulla oblongata, which are involved in pain regulation, inflammation, antioxidation and nitric oxide production, were analyzed by western blot. The degree of orifacial pain was significantly lower in group IV than in groups I, II and, III. The expression of p38MAPK, iNOS and Nrf2 in the brain and medulla were also lower in group IV than in the other groups. These findings suggested that a Hovenia dulcis extract can attenuate inflammatory orifacial pain by suppressing the expression of p38 MAPK, iNOS and Nrf2.

• Journal of the Korean Geotechnical Society
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• v.28 no.1
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• pp.55-66
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• 2012

The load transfer mechanism of hybrid model of soil-nailing and compression anchor is studied in this paper. The hybrid model is composed of an anchor bar (installed at the tip) with two PC strands and a steel bar. It can make active behavior of skin friction by applying the pre-stress. In this paper, the load transfer mechanisms of soil-nailings, compression anchors, and hybrid models, respectively, are obtained from skin friction theory and load transfer theory. Field pullout tests are performed to identify the load transfer mechanism and experimental results are compared with analytical solution. In case of soil-nailings, the tension load is transferred from face to tip, however, in case of compression anchors, the compression load is transferred from tip to face. The experimental behavior of the hybrid model is similar to that of compression anchor when only pre-stress is applied. If the pullout test is performed by simultaneously pulling out the anchor and the nail, the compression load is dominant at the tip and tension load is dominant at the face. The load transfer mechanism of the hybrid model shows the combined behavior of soil-nailings with compression anchors.