• Korean Journal of Clinical Laboratory Science
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• v.51 no.3
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• pp.316-322
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• 2019

Helicobacter pylori, a gram-negative bacterium, is one of the risk factors that induces gastritis and gastric cancer. Therefore, much attention has been paid to the compounds that inhibit bacterial growth or eradicate bacteria. Evodiae fructus (EF), the fruit of Evodia rutaecarpa, has been used for treating diarrhea and abdominal pain. EF extract was already found to inhibit the growth of H. pylori. However, to the best of our knowledge, the effect of EF on the virulence factors of H. pylori has not been reported. In this study, when comparing the minimum inhibitory concentration (MIC) of the different methanol concentration extracts, the 95% methanol extract (EF95) showed the lowest MIC value. EF95 extract suppressed the expressions of cagA, vacA and ureB, but interestingly, it up-regulated the expression of ureA. A decrease in production of ammonia in the culture medium and the cell lysates indicated that EF95 inhibited the urease activity in H. pylori, which was the result of EF95 inhibiting the ureB expression. Although the mechanism by which EF95 extract regulates the virulence factors in H. pylori needs further study, EF95 could be used for treatment of gastric troubles induced by H. pylori.

• Applied Biological Chemistry
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• v.6
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• pp.15-24
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• 1965

The mackerels, Scomber japonicus (HOUTTYN), for this study were caught by purse sein net in Korea. In this paper, the seasonal variations of the oil content and Vitamin A concentration in oil in different parts of the mackerel, the distribution of the Vitamin A of viscera of that and physical and chemical constants of oil were discussed. The results are summarized as follows: 1. In the beginning of June, the liver weight increased rapidly and reached the maximum. In this period, the gonad weight was also maximum. It seemed that this phenomenon has influenced on the nutritional and physiological aspects of the spawning stage. 2. The Vitamin A concentration of liver oil reached the maximum value in the middle of July. In the most case, it was proportinate to the oil content in liver inversely. 3. The Vitamin A concentration of pyloric caeca oil reached the maximum Value in the late of July. It showed the tendency of being proportionate to the oil content in pyloric caeca inversely. And the Vitamin A concentration of intestine oil reached maximum value in the beginning of July. 4. The distribution average ratio of Vitamin A in liver, pyloric caeca, intestine, stomach and contents of stomach and gonad to the total Vitamin A in whole viscera were 60.8, 29.4, 5.7, 2.2, and 1.9 percentage. The seasonal variation of the distribution of Vitamin A in pyloric caeca to the amount of total Vitamin A in whole viscera was proportionate to that of liver inversely. 5. It seemed that there were no any corelation between the Vitamin A content and seasonal variation of the physical and chemical constants of viscera oil. But when the Vitamin A concentration was high, the refractive index, the amount of unsaponifiable matter and iodine value of viscera oil were also high. 6. On the extracting vitamin oil of viscera of mackerel, it is the most suitable period from the end of May to the middle of October. The liver, pyloric caeca and intestine of mackerel are valuable and the other parts of vicera are worthless as vitamin oil resources. It is probable that the whole viscera oil could also be utilized as vitamin-rich oil, if it were concentrated.

• Journal of Dairy Science and Biotechnology
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• v.34 no.1
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• pp.37-41
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• 2016

Melatonin is a hormone produced by the pineal gland in dark conditions. It plays a major role in the regulation of the sleep-wake cycle. Melatonin synthesis is known to be suppressed by environmental light. Cortisol is a steroid hormone that is a major indicator of physiological alterations due to stressful stimuli. It also displays a circadian rhythm, like melatonin. The highest levels are encountered during early morning and the lowest levels are observed at around midnight. In the present study, the effects of milking time on the melatonin and cortisol concentrations of raw milk and milk powder at the summer and winter solstices were examined. The melatonin concentration in milk increased significantly if cows were milked in the dark at night (p<0.05). The melatonin concentration in milk powder showed the same pattern with respect to the milking time (p<0.05). However, no significant difference in the cortisol concentration was observed between day- and night-time milk. Although the time of day did not affect the level of milk cortisol, seasonal factors affected the release of cortisol in milk (p<0.05). In conclusion, night-time milk is rich in endogenous melatonin. In this respect, it has potential applications for the development of melatonin rich-dairy products, which serve as natural sources of melatonin.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.3
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• pp.158-164
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• 2001

The purpose of this study was to assess the runoff of nitrogen derived from pig manure under upland condition. Bare and maize cultivated conditions were compared to estimate the effect of plant on the runoff loss of nitrogen and other nutrients by application of pig manure. Soil used in this experiment was sandy loam, and the fermented pig manure was applied at the rate of 0, 50, and $100ton\;ha^{-1}$. The amount of runoff was measured after every rainfall and water samples were analyzed for nitrate and other cations. Runoff was increased with the rainfall, but was depended on the application rate of pig manure at both bare and maize cultivated plots. Concentrations of nitrate in runoff at 0, 50 and $100ton\;ha^{-1}$ application of pig manure were higher at the maize cultivated plots than those at bare plots by 86.9, 42.9, and 33.6%, respectively. However, total mass of nitrate by runoff loss was higher at the bare plot ranging from 1.34 to $3.15kg\;NO_3-N\;ha^{-1}$. The equivalent ratio of nitrate to sum of cations in runoff was higher at the bare plot than that of maize cultivated plots. The concentration of cations in runoff was in the order of K> Mg> Na> Ca.

• Korean Journal of Weed Science
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• v.5 no.1
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• pp.9-13
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• 1985

This study was conducted to evaluate herbicide resistant plant through tissue culture. Callus was induced from embryos of Echinochloa crusgalli Beauv. (var, oryzicola Ohwi, var. caudata Kitagawa and var, crusgalli). An optium medium for callus induction and succinate dehydrogenase activity in inducted callus were detected and callus growth of various varieties of Echinochloa crusgalli was assessed under the treatment of various rates of butachlor[N-(butoxymethyl)-2-chloro-N-(2,6-diethylphenyl)acetamide]. MS medium seemed to be the most appropriate to induce callus from the embryos of varieties of E. crusgalli by using 2,4-D about 5.5mg/l as a hormone source. The activity of succinate dehydrogenase in inducted callus showed positive reaction against to TTC(2,3,5-triphenyltetrazolium chloride) regardless of concentrations of butachlor and varieties of E. crusgalli, indicating that all the callus induced were alive. The callus growths derived from seeds of E, cnesgalli were greatly affected by various rates of butachlor and were completely inhibited at the highest concentration of butachlor, $10^{-3}M$, regardless of varieties of E. crasgalli. $10^{-6}M$ of butachlor inhibited 24.6% of the callus growth of E. crusgalli Beauv, var. oryzicola Ohwi, while E. crusgalli Beauv. var. crusgalli showed 42% of inhibition, showing that there was difference in response of varieties of E. crusgalli Beauv. to butachlor.

• Journal of Bio-Environment Control
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• v.21 no.4
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• pp.317-321
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• 2012

This study was carried out to investigate the effect of EC levels in nutrient solution on the growth of rose in coir substrate during the juvenile. Nutrient concentration were EC 0.6, 1.0, 1.4, and $1.8dS{\cdot}m^{-1}$. In spite of high concentration of nutrient solution was supplied, EC and inorganic ions content in the extract of substrate was no difference by 22 days after planting. After that, they was rapidly increased with higher concentration of nutrient solution. Number of shoot was highest in EC $1.8dS{\cdot}m^{-1}$ at 2nd growth cycle, 90 days after planting, after that was showed a tendency to increase with increasing nutrient concentration, but was no significant difference among treatment except EC $0.6dS{\cdot}m^{-1}$. Judging by results of growth of rose and contents of inorganic ion in extracts of media, our experiment suggests that the suitable nutrient concentration is EC $1.8dS{\cdot}m^{-1}$ until 90 days and then EC $1.4dS{\cdot}m^{-1}$ until 165 days after planting that is more higher than conventional nutrient concentration for absorption by coir.

• Applied Biological Chemistry
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• v.51 no.3
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• pp.228-232
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• 2008

In the present study, we investigated the glutathione S-transferase (GST) induction of medicinal plants using a cultured PC12 cells. The methanol extracts of Dendrobium nobile Lindley, Schizonepeta tenuifolia Briquet, Glycyrrhiza uralensis Fischer, Paenoia lactiflora Pall were tested. As the result, exposure of PC12 cells to G. uralensis resulted in the significant induction of GST. On the continuous experiment, G. uralensis was extracted with methanol, ethanol, and acetone. Among these extracts, methanol extracts showed the highest GST induction. The methanol extracts were further fractionated with hexane, diethyl ether, ethyl acetate, and water layer according to the degree of polarity. The diethyl ether layer showed the highest exhibited GST induction on PC12 cells. Based on these results suggest that the extracts of G. uralensis can be applicable for the development of a new antioxidant agent.

• KSBB Journal
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• v.20 no.2 s.91
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• pp.84-87
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• 2005

Toxicity test for biodiesel (BD), biodiesel-derived neopentyl polyol ester (NPE) lubricant oil base, lubricant oil for diesel engine oil (LODE) and petroleum diesel (PD) was carried out using earthworm, Eisenia fefida. According to the method by OECD 207, the $LC_{50}$ values of BD and NPE were estimated as 2,450 and 1,528 mg/kg, respectively, which indicate that these compounds are classified as slightly toxic compounds. The $LC_{50}$ values of LODE and PD were estimated as 500 and 603mg/kg, respectively, showing that theses compounds are evaluated as moderately toxic compounds.

• KSBB Journal
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• v.20 no.1 s.90
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• pp.55-59
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• 2005

Toxicity test for biodiesel (BD), biodiesel-derived neopentyl polyol ester (NPE) lubricant oil base, lubricant oil for diesel engine (LODE) and petroleum diesel (PD) was carried out using microalgae, Chlorella vulgaris. According to the method of OECD 201, the $EC_{50}$ values of BD and NPE were estimated as 84 and 69 mg/L, respectively, which indicates that these compounds are classified as slightly toxic compounds. The $EC_{50}$ values of LODE and PD were measured as 42 and 24 mg/mL, respectively, showing that these compounds are considered as moderately toxic compounds.

• Korean Journal of Food Science and Technology
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• v.34 no.3
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• pp.437-443
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• 2002

Using agar and gelatin as wall materials, onion oil was microencapsulated using the extrusion spraying technology. A sensitive methodology was developed for quantitative determination of the microencapsulation yield through ethyl acetate extraction and gas chromatographic analyses. Optimal conditions for the microencapsulation process consisting of the ratio of [core material, Cm] to [wall material, Wm] ($X_1$), temperature of dispersion fluid ($X_2$), detergent concentration in dispersion fluid ($X_3$), and concentration of emulsifier $(X_4)$ were determined using response surface methodology. The regression model equation for the yield of microencapsulation (Y, %) of onion oil could be predicted as $Y\;=\;97.028571-0.775000\;(X_1)-0.746726\;(X_1){\cdot}(X_1)\;-\;1.100000\;(X_3){\cdot}(X_2)$. The optimal conditions for the microencapsulation of the onion oil were determined as the ratio of [core material] to [wall material] of 4.5 : 5.5 (w/w), the temperature of dispersion fluid of $17.1^{\circ}C$ detergent concentration in dispersion fluid of 0.03%, and the concentration of emulsifier of 0.42%. Results revealed the most stable microcapsule of onion oil could be formed with the highest yield of microencapsulation (more than 95%) under optimal conditions.