• Membrane Journal
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• v.15 no.3
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• pp.224-232
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• 2005

The use of the lung assist device (LAD) would be well suited for acute respiratory failure (ARF) patients, combining the simplicity of mechanical ventilation with the ability of extracoporeal membrane oxygenators (ECMO) to provide temporary relief for the natural lungs. This study's specific attention was focused on the effect of membrane vibration in the LAD. Quantitative experimental measurements were performed to evaluate the performance of the device, and to identify membrane vibration dependence on blood hemolysis. We tried to decide upon excited frequency band of limit hemolysis when blood hemolysis came to through a membrane vibration action. The excited frequency of the module type 5, consisted of 675 hollow fiber membranes, showed the maximum gas transfer rate. We concluded that the maximum oxygen transfer rate seemed to be caused by the occurrence of maximum amplitude and the transfer of vibration to hollow fiber membranes. It was excited up to $25{\pm}5$ Hz at each blood flow rate of module type 5. We found that this frequency became the 2nd mode resonance riequency of the flexible in blood flow. Blood hemolysis was low at the excited frequency of $25{\pm}5$ Hz. Therefore, we decided that limit hemolysis frequency of this LAD was $25{\pm}5$ Hz.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.399-405
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• 2018

The platelet count in clinical laboratories is essential for the diagnosis and treatment of hemostasis abnormalities, and accurate platelet counting in the low count range is of prime importance for deciding if a platelet transfusion is needed and for monitoring after chemotherapy. Quality control is designed to reduce and correct any deficiencies in the internal analytical process of a clinical laboratory prior to the release of patient results. Fragmented erythrocytes are the major confusing factors for platelet counting because of their similar size to platelets. The authors found that the low range QC values were out of 2SD with a Sysmex automatic analyzer in internal quality control process. Thus far, there has been little discussion on the relationship between hemolysis and the platelet parameters. Therefore, this study focused on the performance of automated platelet counts, including the PLT-F, the PLT-I, and PLT-O methods at the low platelet range using the low level QC materials and compared the 5 platelet parameters with the hemolyzed samples. The results showed that the CV was the smallest with PLT-F and P-LCR increased from 18.4 to 31.9% in the hemolysis samples. These results indicate that a more accurate estimation of the platelet counts can be achieved using the PLT-F method than the PLT-I method at the low platelet range. The use of the PLT-F system improves the confidence of results in low platelets samples in a routine hematology laboratory. The results suggest that P-LCR is a new parameter in assessing samples when the specimen is suspected of hemolysis and deterioration. Nevertheless, further studies will be needed to establish the relationship with P-LCR and hemolysis using human blood specimens.

• The Korean Journal of Physiology
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• v.17 no.2
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• pp.135-142
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• 1983

$PGE_2$ and $PGF_{2{\alpha}}$ are known to act similarly in a number of animal tissues. They both facilitate regression of corpus luteum(Poyser, 1972; Fuch et al, 1974; Coudert et at, 1974) and stimulate contraction of uterine muscle (Laudanski et al, 1977; Porter et al, 1979; Hollingsworth et al, 1980). It is, however, not known whether these two prostaglandins exert similar actions in osmotic fragility of erythrocytes (Rasmussen et al, 1975) and $PGF_{2{\alpha}}$ alters conformation of membrane proteins (Meyers aud Swislocki, 1974). The former effect may not be mediated through changes in c- AMP concentration in the cell, since the adenylate cyclase activity in human erythrocyte is extremely low (Rodan et al, 1976; Sutherland et al, 1962) and the latter effect implies that physical state (or fluidity) of the membrane is altered by $PGF_{2{\alpha}}$. The present study was undertaken to elucidate mechanisms of action of $PGE_2$ and $PGF_{2{\alpha}}$ on the human erythocyte membrane by examining their effects on osmotic fragility and $Ca^{++}$ binding to the membrane fragments. The results are summarized as follows: 1) $PGE_2$ and $PGF_{2{\alpha}}$ increased osmotic fragility at concentrations above $10^{11}\;M$, the effect being similar for both hormones. The concentration of NaCl for 100% hemolysis was $1/16{\sim}1/17\;M$ in the presence of $10^{11}\;M\;PGE_2$ or $PGF_{2{\alpha}}$ and 1/18 M in the absence of the hormone (control). 2) When erythrocytes were suspended in 1/15 M NaCl solution, $44.2{\pm}4.3%$ of cells were hemolyzed. Addition of $10^{12}\;M\;PGE_2$ or $PGF_{2{\alpha}}$ did not increase hemolysis. When the concentration of the hormones was increased to $10^{11}\;M$, however the degree of hemolysis increased markealy to about 80%. No further increase in hemolysis was observed at concentration of the hormones above $10^{11}\;M$. 3) The additional hemolysis due to $10^{11}\;M\;PGE_2$ and $PGF_{2{\alpha}}$ appeared to he identical regardless of absence or presence of $Ca^{++}\;(0.5{\sim}10\;mM)$ in the suspending medium. 4) In the absence of prostaglandin, the binding of $Ca^{++}$ to the erythrocyte membrane increased curvilinearly as the $Ca^{++}$ concentration increased up to 5 mM above which it leveled off. A similar dependence of $Ca^{++}$ binding on the $Ca^{++}$ concentration was observed in the presence of $10^{11}\;M\;PGE_2$ or $PGF_{2{\alpha}}$, however, the amount of $Ca^{++}$ bound at a given $Ca^{++}$ concentration was significantly higher than in the absence of the hormones. 5) As in the hemolysis, $PGE_2$ and $PGF_{2{\alpha}}$ did not affect the $Ca^{++}$ binding at a concentration of $10^{12}\;M$, but increased it by about 100% at concentration above $10^{11}\;M$. These result indicate that both tile osmotic fragility of erythrocyte and the $Ca^{++}$ binding to the erythrocyte membrane are similarly enhanced by $PGE_2$ and $PGF_{2{\alpha}}$, but these two effects are not causally related. It is, therefore, concluded that the prostaglandin-induced hemolysis is not directly associated with alterations of the $Ca^{++}$ content in the membrane.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.175-175
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• 1994

녹용의 유효성분을 분리하기 위하여 지질성분을 분석하고 그 약효를 검색하였다. Folch-Suzuki 분배법, Sephadex G-50, DEAE-Sephadex A-25 Column Chromatography, High Performance Thin Layer Chromatography, HPLC로 정제한후 Mass Spectrometer, NMR, FT-IR등을 이용하여 구조를 분석하였다. 이 물질은 분자량이 1065인 polyhydroxyunsaturated lipid임을 확인하였다. Strepthzotocin으로 당뇨병을 유발시킨 쥐에 이 물질을 투여한 후 혈액과 조직에서 생화학적 변화를 조사하였다. 정상군에 비해 투여군에서 혈당이 감소하였으나 혈액에서 insulin의 양은 증가하지 않았다. 당뇨병 쥐의 적혈구가 정상에 비해 용혈이 되지 않으나 투여군의 적혈구는 정상과 비숫한 용혈현상을 보여 주었다. 대뇌조직에서 gangliosides를 분석한 결과 당뇨병에 의해 GM1이 증가하는 양상을 보여 주었으나 투여군의 경우 대뇌 gangliosides 분포는 정상과 같았다. 대뇌 lipid bound sialic acid를 정량한 결과 당뇨병에 의해 그 양이 감소되었으나 녹용을 투여하였을 때는 정상에 비해 그 양이 더 증가하였다. Strepthzotocin으로 유발시킨 당뇨병 쥐에 녹용의 지질성분을 투여하였을 때 치료효과가 있는 것으로 추정된다.

• 순환기질환의공학회:학술대회논문집
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• 2002.11a
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• pp.54-55
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• 2002

• Korean Journal of Food Science and Technology
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• v.35 no.3
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• pp.510-518
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• 2003

Protective effects of natural components including genistein (4',5,7-trihydroxyisoflavone) from Glycine max MERRILL on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. Genistein $(10{\sim}100\;{\mu}m)$ suppressed photohemolysis in a concentration-dependent manner, and was more effective than the lipid peroxidation chain blocker, ${\alpha}$-tocopherol (Vit. E). Glycoside of genistein, genistin, the water-soluble antioxidant, L-ascorbate, and the iron chelator, myo-inositol hexaphosphoric acid dodecasodium salt (sodium phytate) did not exhibit protective effect against photohemolysis. L-Ascorbate and sodium phytate stimulated photohemolysis at high concentration $(500\;{\mu}m)$. ${\alpha}$-Carotene 3,3'-diol (lutein), a singlet oxygen $(^1O_2)$ quencher, exhibited pronounced protective effect, an indication that $^1O_2$ is important in photohemolysis sensitized by rose-bengal. Reactive oxygen scavenging activities $(OSC_{50})$ of natural antioxidants including genistein on reactive oxygen species (ROS) generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescence assay were in the order of sodium phytate > L-ascorbate > ${\alpha}$-tocopherol > genistein > genistin. $OSC_{50}$ value of genistein, genistin, ${\alpha}$-tocopherol, L-ascorbate, and sodium phytate were 41.0, 109.0, 9.0, 5.2, and $0.56{\mu}m$ respectively. The order of free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity $(FSC_{50})$ was L-ascorbate > ${\alpha}$-tocopherol > genistein > genistin. These results indicate that genistein can function as an antioxidant in biological systems, particularly skin exposed to solar UV radiation by scavenging $^1O_2$ and other ROS, and to protect cellular membranes against ROS.

• Journal of Applied Biological Chemistry
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• v.59 no.3
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• pp.221-225
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• 2016

Tolaasin is a pore-forming peptide toxin produced by Pseudomonas tolaasii and causes a brown blotch disease by disrupting membrane structures of cultivated mushrooms. The mechanism and characteristics of tolaasin pore formation are not known in detail; however, tolaasin pores have been demonstrated in the artificial lipid bilayer. Since the tolaasin pore appeared less frequently and unstable in lipid bilayer, a mismatch between the length of tolaasin pore and the thickness of lipid membrane had been suggested. Therefore, tolaasin-induced hemolyses were measured by the additions of phospholipids composed of various fatty acids with different carbon numbers. When phosphatidylethanolamines made with two decanoic acids (C10:0, 1,2-didecanoyl-sn-glycero-3-phosphoethanolamine; DDPE), myristic acids (C14:0, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine), and stearic acids (C18:0, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine) were added to the buffer containing RBCs and tolaasin peptides, DDPE facilitated the tolaasin-induced hemolysis while the other two phospholipids showed no effects. At various concentrations of DDPE, the tolaasin-induced hemolysis was stimulated as a dose-dependent manner. The phospholipids composed of mediumchain fatty acids stabilize the tolaasin pore probably by binding between the pore structure and membrane phospholipids and making the membrane thickness thinner around the pore. These results showed that tolaasin molecules make more stable pores in the membrane made with phospholipids composed of medium length fatty acids, suggesting that the length of tolaasin pore is a little shorter than the thickness of RBC membrane.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.6
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• pp.545-550
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• 2002

A hemolysin producing bacterial strain which belong to Vibrio species was isolated from the Kum River estuary. In the process of identification, the strain did not show characteristics of known Vibrio species; thus, the strain was designated as Vibrio sp, E10 (V. kunsan) tentatively and further identification study was carried out by comparing its bacteriological characteristics. Morphologically Vibrio sp, E10 was comma shaped rod with a polar flagellium. Clear hemolysis zones were observed with the strain against human and sheep blood agar. Hemollytic toxicity was confirmed by strong vascular Permeability and fatal toxicity against mouse was also observed. Therefore the strain was a pathogenic vibrio. Growth conditions for Vibrio sp. E10 were ranged salinity of 0$\~$$4.5\%$, pH of 6.2$\~$9.2, temperature of 14$\~$42$^{\circ}C$, respectively, 16S rDNA partial sequence of Vibrio sp, E10 showed $99\%$ homology with dozens of V. cholerae species including V, cholerae El Tor N16961 and V, snmisnfus ATCC 33653T. This strain belonged to Proteobacteria; gamma subdivision; Vibrionacea: Vibrio. But, among knorn Vibrio species no identical styains were found when using automatic bacteria identification system ($MicroLog^{TM}$system, release 4.0, Biolog Inc., USA) which evaluated the ability of metabolizing 95 kinds of carbon and nitrogen sources. Vibrio sp, E10 showed 18 and 11 different responses as compared to V. mimicus and V, cholerae, respectively.

• Journal of Food Hygiene and Safety
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• v.1 no.2
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• pp.143-149
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• 1986

Paraquat의 만성독성에 대한 vitamin E 급여의 효과를 조사하기 위하여 랫드에 paraquat를 50 ppm의 농도로 희석한 물을 음수 대신 투여하면서 vitamin E 함유 사료를 급여하여 paraquat에 대한 vitamin E의 방어 효과를 측정하였는 바 다음과 같은 결과를 얻었다. 1.혈장 vitamin E 농도는 사료 kg 당 10 mg까지의 vitamin E 함유 사료의 급여로 약 150$\mu\textrm{g}$/100ml까지 급격히 상승하였으며 그 후 급여량의 증가에도 비교적 안정된 혈중 농도를 유지하였고, 또한 paraquat 투여는 vitamin E 급여량에 관계없이 혈중 vitamin E 농도의 감소를 가져왔다. 2. 간저장 vitamin E 농도는 혈장 vitamin E 농도와 달리 급여량의 증가에 따라 비례하여 증가하는 경향을 보였으며 paraquat 투여에 의한 영향이 적었다. 3. Paraquat 투여는 적혈구 용혈율을 크게 증가시켰음 (95%), 100$\mu\textrm{g}$/100ml 이상의 vitamin E 혈장 농도에서는 안정된 적혈구 용혈율 (10% 이하)을 나타내었다. 4. Paraquat 투여는 혈장 trypsin inhibitor capacity를 크게 증가시켰으며, vitamin E 급여에 의하여 다소 회복되는 경향을 보였으나 정상에 이르지 못하였다. 5. 혈장 및 적혈구 glutathione peroxidase 활성은 paraquat 투여에 의하여 영향을 받지 않았다. 6. paraquat 투여 후의 lipid peroxide는 vitamin E의 급여로 감소되었다.

• Journal of Korean Clinical Nursing Research
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• v.17 no.3
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• pp.399-410
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• 2011

Purpose: This study was conducted to investigate the incidence of blood hemolysis and repeated blood sampling and to identify factors contributing to hemolysis and repeated blood sampling in the emergency department. Methods: A cross-sectional descriptive design was used. Participants were the patients who came to emergency department and are required a blood sampling for electrolyte level. All blood samples were collected by emergency department nurses and determined for hemolysis by experienced laboratory technologists. Data were analyzed using $x^2$-test, Fisher's exact test, Mann-Whitney u test and Binary Logistic Regression to determine significant differences. Results: A total of 402 valid samples were collected. Of these, 30 blood samples (7.5%) were found to be hemolyzed and 9 (2.2%) to be recollected. Statistically significant factors affecting on hemolysis and repeated blood sampling included the time of bloods sampling (night), the time of tourniquet application, and too-fast blood draw into the test tube. Conclusion: We recommend that nurses who take the blood sampling to consider the findings of the study and take the related factors into account as they set up the standardized care protocol in order for nursing quality improvement.