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http://dx.doi.org/10.22650/JKCNR.2011.17.3.10

Factors Affecting Hemolysis in Blood Sampling and Repeated Sampling in the Emergency Department  

Cho, Myung Sook (Department of Clinical Nursing Science, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Hwang, Moon Sook (Department of Nursing Science, School of Oriental Medicine in Woosuk University)
Publication Information
Journal of Korean Clinical Nursing Research / v.17, no.3, 2011 , pp. 399-410 More about this Journal
Abstract
Purpose: This study was conducted to investigate the incidence of blood hemolysis and repeated blood sampling and to identify factors contributing to hemolysis and repeated blood sampling in the emergency department. Methods: A cross-sectional descriptive design was used. Participants were the patients who came to emergency department and are required a blood sampling for electrolyte level. All blood samples were collected by emergency department nurses and determined for hemolysis by experienced laboratory technologists. Data were analyzed using $x^2$-test, Fisher's exact test, Mann-Whitney u test and Binary Logistic Regression to determine significant differences. Results: A total of 402 valid samples were collected. Of these, 30 blood samples (7.5%) were found to be hemolyzed and 9 (2.2%) to be recollected. Statistically significant factors affecting on hemolysis and repeated blood sampling included the time of bloods sampling (night), the time of tourniquet application, and too-fast blood draw into the test tube. Conclusion: We recommend that nurses who take the blood sampling to consider the findings of the study and take the related factors into account as they set up the standardized care protocol in order for nursing quality improvement.
Keywords
Blood specimen collection; Hemolysis;
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1 박순철, 신영호, 김정림, 문영아, 공보경, 한혜연 등(2009). 재 채혈 감소및환자만족도 향상을 위한개선활동.한국의료QA학회가을학술대회자료집, 545-547.
2 박성현,조신섭, 김성수(2009). SPSS (PASW) 17.0이해와 활용. 서울: 한나래출판사.
3 송경애, 이우숙, 박형숙, 윤은주, 이강이, 홍영혜 등(2009). 기본간호중재의 적용(개정판). 서울: 수문사.
4 심효진(2008). 혈액검사물의 재채혈건수 감소. 한국의료QA학회 가을학술대회자료집, 444-446.
5 육근돌(1998). 자동혈구계산기를 이용한 혈소판 수 측정에서 용혈의 영향. 충남대학교 석사학위논문, 대전.
6 윤영진(2007). 의료기기의 마케팅전략에 관한 연구 -진공채혈관 사례를 중심으로-. 한양대학교 석사학위논문, 서울.
7 지제근(편.)(2009). 지제근 의학용어사전(제2판). 서울: 아카데미아.
8 홍숙희, 김용호(1981). 용혈이 각종 생화학 검사결과에 미치는 영향. 인제의학, 2 (3), 345-350.
9 Berger-Achituv, S., Budde-Schwartzman, B., Ellis, M.H., Shenkman, Z., & Erez, I. (2010). Blood sampling through peripheral venous catheters is reliable for selected basic analytes in children. Pediatrics, 126 (1), e179-186.   DOI   ScienceOn
10 Cox, S. R., Dages, J. H., Jarjoura, D., & Hazelett, S. (2004). Blood samples drawn from IV catheters have less hemolysis when 5-ml (vs 10-ml) collection tubes are used. Journal of Emergency Nursing, 30 (6), 529-533.   DOI   ScienceOn
11 Dugan, L., Leech, L., Speroni, K. G., & Corriher, J. (2005) Factors affecting hemolysis rates in blood samples drawn from newly placed IV sites in the emergency department. Journal of Emergency Nursing, 31 (4), 338-345.   DOI   ScienceOn
12 Fang, L., Fang, S. H., Chung, Y. H., & Chien, S. T. (2008). Collecting factors related to the haemolysis of blood specimens. Journal of Clinical Nursing, 17 (17), 2343-2351.   DOI   ScienceOn
13 Fernandes, C. M., Walker, R., Price, A., Marsden, J., & Haley, L. (1997). Root cause analysis of laboratory delays to an emergency department. The Journal of Emergency Medicine, 15 (5), 735-739.   DOI   ScienceOn
14 Grant, M. S. (2003). The effect of blood drawing techniques and equipment on the hemolysis of ED laboratory blood samples. Journal of Emergency Nursing, 29 (2), 116-121.   DOI   ScienceOn
15 Kennedy, C., Angermuller, S., King, R., Noviello, S., Walker, J., Warden, J., et al. (1996). A comparison of hemolysis rates using intravenous catheters versus venipuncture tubes for obtaining blood samples. Journal of Emergency Nursing, 22 (6), 566-569.   DOI   ScienceOn
16 Lowe, G., Stike, R., Pollack, M., Bosley, J., O'Brien, P., Hake, A., et al. (2008). Nursing blood specimen collection techniques and hemolysis rates in an emergency department:Analysis of venipuncture versus intravenous catheter collection techniques. Journal of Emergency Nursing, 34 (1), 26-32.   DOI   ScienceOn
17 Saleem, S., Mani, V., Chadwick, M, A., Creanor, S., & Ayling, R. M. (2009). A prospective study of causes of haemolysis during venepuncture: Tourniquet time should be kept to a minimum. Annals of Clinical Biochemistry, 46 (Pt 3), 244-246.   DOI
18 Ong, M. E., Chan, Y. H., & Lim, C. S. (2008). Observational study to determine factors associated with blood sample haemolysis in the emergency department. Annals of the Academy of Medicine Singapore, 37 (9), 745-748.
19 Ong, M. E., Chan, Y. H., & Lim, C. S. (2009). Reducing blood sample hemolysis at a tertiary hospital emergency department. The American Journal of Medicine, 122 (11), 1054. e1-1050.e6.   DOI
20 Paoloni, R., Taghizadeh, M., Kouzios, D., & Janu, M. (2010). Blood withdrawn through a cannula valve connector does not result in clinically significant haemolysis. Emergency Medicine Australasia, 22 (4), 310-315.   DOI   ScienceOn
21 Sharp, M. K., & Mohammad, S. F. (1998). Scaling of hemolysis in needles and catheters. Annals of Biomedical Engineering, 26 (5), 788-797.   DOI