[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5657/kfas.2002.35.6.545

Bacteriological Characteristics of Unidentified Vibrio sp., Hemolysin Producer Isolated from Brackish Water -2. Bacteriological Characteristics of Vibrio sp. E10 Similar to Vibrio mimicus-

Kim Young Man (Department of Food Science and Nutrition, Dong-eui University)
Yu Hong Sik (Watervision Co.,)
Oh Hee Kyung (Department of Food Science and Nutrition, Dong-eui University)

Publication Information

Korean Journal of Fisheries and Aquatic Sciences / v.35, no.6, 2002 , pp. 545-550 More about this Journal

Abstract

A hemolysin producing bacterial strain which belong to Vibrio species was isolated from the Kum River estuary. In the process of identification, the strain did not show characteristics of known Vibrio species; thus, the strain was designated as Vibrio sp, E10 (V. kunsan) tentatively and further identification study was carried out by comparing its bacteriological characteristics. Morphologically Vibrio sp, E10 was comma shaped rod with a polar flagellium. Clear hemolysis zones were observed with the strain against human and sheep blood agar. Hemollytic toxicity was confirmed by strong vascular Permeability and fatal toxicity against mouse was also observed. Therefore the strain was a pathogenic vibrio. Growth conditions for Vibrio sp. E10 were ranged salinity of 0 $\~$ $4.5\%$ , pH of 6.2 $\~$ 9.2, temperature of 14 $\~$ 42 $^{\circ}C$ , respectively, 16S rDNA partial sequence of Vibrio sp, E10 showed $99\%$ homology with dozens of V. cholerae species including V, cholerae El Tor N16961 and V, snmisnfus ATCC 33653T. This strain belonged to Proteobacteria; gamma subdivision; Vibrionacea: Vibrio. But, among knorn Vibrio species no identical styains were found when using automatic bacteria identification system ( $MicroLog^{TM}$ system, release 4.0, Biolog Inc., USA) which evaluated the ability of metabolizing 95 kinds of carbon and nitrogen sources. Vibrio sp, E10 showed 18 and 11 different responses as compared to V. mimicus and V, cholerae, respectively.

Keywords

Unidentifed Vibrio sp; Brackish water; Hemolysis; Pathogenic vibrio; 16S rDNA;

Citations & Related Records

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