• Title/Summary/Keyword: 용존단백질

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포말분리기에 의한 해수 중의 단백질 및 부유 고형물 제거

  • 김병진;서근학;김성구
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.235-236
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    • 2000
  • 최근 경제 성장과 더불어 고단백, 저지방 식품인 어류의 수요가 증대되고 있으며, 특히 횟감용 활어의 소비량이 급증하고 있다. 반면 해수의 수급이 용이하지 않은 내륙지방의 경우 활어수조 내 해수의 오염도가 높아 어류의 폐사가 발생하며, 이로 인해 국민건강에 영향을 미친다. 활어 수조 내에서 발생되는 어류의 배설물 중 단백질 성분은 미생물에 의해 분해되어 암모니아를 발생시키고 용존산소를 감소시키는 물질로서 신속한 제거가 필요하다(서 등, 1999). (중략)

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포말분리에 의한 해수 내 양어장 오염물 제거시 유입 단백질 농도 영향

  • 이정훈;김병진;서근학
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.326-327
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    • 2001
  • 양어장 내에서 발생되는 어류의 배설물 중 단백질 성분은 미생물에 의해 분해되어 암모니아를 발생시키고 용존산소를 감소시키는 물질인 반면 소수기와 친수기를 함께 가지는 구조적 특성으로 인해 계면활성제 역할을 하여 별도의 계면활성제를 첨가하지 않아도 포말분리를 수행 할 수 있도록 해줌으로, 포말분리에 의한 양어장 순화수 중 어류에 유해한 성분의 처리수단으로써 많이 연구, 적용되어지고 있다. (중략)

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The Effect of Dissolved Oxygen and Salinity on Oxygen Consumption and Ammonia Excretion in the Mysid, Neomysis awatschensis (곤쟁이 (Neomysis awatschensis)의 산소소비와 질소배설에 미치는 염분 및 용존산소의 영향)

  • SHIN Yun Kyung;LEE Jung Ah;CHIN Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.2
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    • pp.110-114
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    • 2000
  • Effects of the combinations of six oxygen concentrations ($control, 0.6, 1.0, 2.0, 3.4 and 7.4 mg DO/l$) and two salinity levels ($20{\%_{\circ}} and 32{\%_{\circ}}$) on the rates of oxygen consumption, ammonia excretion and mortality of the mysid, Neomysis awatschensis were tested at $20{\circ}C$. The lethal level ($96 hr-LC-(50)$) of dissolved oxygen for mysid at $20{\%_{\circ}} and 32{\%_{\circ}} were 2,20 mg DO/l and 1.60 mg DO/l$ respectively, and all mysids died within $24hr at 0.6 mg DO/l$. Oxygen consumption rate of mysid was increased with dissolved oxygen increase at $20{\%_{\circ}} and 32{\%_{\circ}}$, but ammonia excretion rate was high af $1.0 mg DO/l$ during 96h exposure to DO concentration, and significantly greater in $20{\%_{\circ}} than 32{\%_{\circ}}$. $O:N$ ratio of mysid exposed during 96hr with salinity anil dissolved oxygen was below $10 at 20{\%_{\circ}} and 1,0{\~}2.0 mg DO/l, and was 4.4 at 32{\%_{\circ}} and 1.0 mg DO/l$. These results indicated that mysids were capable of changing their energy substrate in response to salinity and DO changes, and obtaining energy from proteins.

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Monitoring of pH and dissolved oxygen in microorganism fermentation processes using a 24-well microplate (24-웰 마이크로 플레이트를 이용한 미생물 발효공정에서 pH 및 용존산소의 모니터링)

  • Kim, Sun-Yong;Kim, Chun-Kwang;Sohn, Ok-Jae;Rhee, Jong-Il
    • KSBB Journal
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    • v.24 no.2
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    • pp.207-211
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    • 2009
  • In this study, the sensing membranes for detection of pH and dissolved oxygen(DO) were prepared by immobilizing 6-aminofluorescein or ruthenium complex onto the sol-gel matrixes of GPTMS, MTMS, and TEOS and then recoated with the mixture of hydrophobic sol-gel and graphite for light insulation. The pH and DO sensing membranes recoated with the light insulation layer showed a higher sensitivity than those without light insulation layer. The sensing membranes were immobilized on the wells of 24-well microplate and used to monitor the fluorescence intensity for pH and DO in E.coli JM109 and P.pastoris X-33 fermentation processes. The change of the fluorescence intensity in the DO sensing membrane agreed with the growth patterns of microorganisms, that the membranes are valuable to monitor the DO in fermentation processes. In the case of pH monitoring, the fluorescence intensity has showed good correlation to the off-line pH data, that the pH membranes are valuable to monitor pH values in fermentations.

Effect of Sonification on the Ananerobic Digestion of Waste Activated Sludge(I) -Disintegration of Waste Activated Sludge Using Ultrasonic and Alkaline Pre-treatments- (초음파가 폐활성 슬러지의 혐기성 소화에 미치는 영향(I) -초음파 및 알칼리 전처리를 이용한 폐활성 슬러지의 가용화-)

  • Han, Sun-Kee;Lee, Chae-young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.17 no.1
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    • pp.96-102
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    • 2009
  • The effect of ultrasonic and alkaline pre-treatments on waste activated sludge (WAS) disintegration was investigated for improved anaerobic digestion. As WAS was treated by either methods, longer capillary suction time (CST) was required due to the break-up of cell walls, and its supernatant demonstrated increase in soluble chemical oxygen demand (SCOD), protein content and turbidity. Ultrasonic process combined with alkaline pre-treatment demonstrated higher SCOD and protein content in the supernatant as compared with ultrasonic pre-treatment only. However, the degree of disintegration (DDCOD) of WAS decreased with increasing solid concentration as both WAS disintegration methods employed simultaneously.

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해수 포말분리에서 공기분산기 기공크기의 영향

  • 김병진;서근학;김용하;김성구
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.10a
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    • pp.211-212
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    • 2000
  • 최근 경제 성장과 더불어 고단백, 저지방 식품인 어류의 수요가 증대되고 있으며, 특히 횟감용 활어의 소비량이 급증하고 있다. 반면 해수의 수급이 용이하지 않은 내륙지방의 경우 활어수조 내 해수의 오염도가 높아 어류의 페사가 발생하며, 이로 인해 국민건강에 영향을 미친다. 활어 수조 내에서 발생되는 어류의 배설물 중 단백질 성분은 미생물에 의해 분해되어 암모니아를 발생시키고 용존산소를 감소시키는 물질로서 신속한 제거가 필요하다(서 등, 1998). (중략)

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Physico-Chemical Characteristics of Sewage Sludge under Electron Beam Irradiation (전자선으로 처리한 하수슬러지의 특성연구)

  • Shin, Kyung-sook;Kang, Ho;Bang, Ky-youn
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.7
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    • pp.1225-1232
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    • 2000
  • This study was carried out to define the effect of electron beam irradiation on the physico-chemical characteristics of sewage sludges. The experimental evidence showed that both pH and alkalinity of irradiated sludge were generally increased as the dose of irradiation increased. It was found that the soluble protein concentration (SPC) and soluble chemical oxygen demand (SCOD) from the sludge right after electron beam irradiation at 3kGy(kilo-joule/kg) increased 2.2 times and 10 times respectively more than those sludges without electron beam treatment. This highly solubilized organics could be resulted in a good soluble substrate for the subsequent anaerobic digestion process. The specific resistance of filtration (SRF) tests showed that sludge dewaterability under electron beam irradiation at 6kGy was found to be 8.8 times higher than that of unirradiated sludge. The sludge dewaterability seemed to be directly related to the dosage of electron beam irradiation up to 10kGy. However, the efficiency of sludge dewaterability tended to be smaller with higher applied irradiation dose. In comparing treatments by different inorganic chemical conditioner with irradiated and unirradiated sludges, it appeared that the dewaterability with irradiated sludge was approximately 4-10 times better that that of unirradiated sludge. Even electron beam treatment itself could replace the result from the sludge conditioned with inorganic chemical coagulants.

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Insect Cell Cultures for Recombinant Protein Production (재조합 단백질 생산을 위한 곤충세포의 배양)

  • 박영민;정용주양재명정인식
    • KSBB Journal
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    • v.4 no.3
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    • pp.266-270
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    • 1989
  • Insect cell cultures were performed in laboratory-scale vessels. The batch growth of insect cells was affected by such parameters as serum content, other nutrients, seeding density, and mechanical agitation. Lactate and ammonium were not likely to be environmental factors that inhibited cell growth at the concentrations observed at the end of batch cultures. In addition, redox potential was found to be a useful index in monitoring low-level dissolved oxygen during the cultivation of insect cells. Recombinant protein production by cells infected with a genetically-modified baculovirus was also demons treated. The maximum beta-galactosidase synthesis of 2800 units per reactor volume was achieved at the dilution rate of $0.006hr^{-1}$.

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Changes in Spectroscopic Characteristics and Pyrene Binding Reactivities of Dissolved Organic Matters By Biodegradation (생분해에 의한 용존 자연유기물질 분광특성 및 Pyrene 결합반응성 변화)

  • Park, Min-Hye;Hur, Jin
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.9
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    • pp.893-899
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    • 2008
  • Changes in spectroscopic characteristics and pyrene binding coefficients of terrestrial dissolved organic matters(DOM) were investigated during microbial incubation. The incubation studies were conducted for 21 days using a leaf litter DOM and a soilderived DOM with an inoculum from a river. The dissolved organic carbon(DOC), the specific UV absorbance(SUVA), the synchronous fluorescence spectra, and the pyrene organic carbon-normalized binding coefficient(K$_{oc}$) of the DOM were measured at the incubation days of 0, 3, 7, 14 and 21. After the 21-day incubation, DOC were reduced to 61% and 51% of the original concentrations of the litter DOM and the soil-derived DOM, respectively. Comparison of the spectroscopic characteristics before and after the incubation revealed that the SUVA, the fulvic-like fluorescence(FLF), the humic-like fluorescence(HLF) of the different DOM were enhanced by the incubation whereas the protein-like fluorescence(PLF) was reduced. This indicates that more aromatic and humic-like compounds were enriched during the biodegradation process while biodegradable and weak carbon structures were depleted. Irrespective of the DOM sources, SUVA values showed a positive relationship with pyrene K$_{oc}$ with a correlation coefficient of 0.97. The FLF and HLF also exhibited good correlations with K$_{oc}$ values although different regression equations were obtained from the different DOM. Our results suggest that the selected spectroscopic characteristics could be good estimation indices for the changes of the binding reactivity of DOM for hydrophobic organic contaminants during biodegradation process.

항류 공기 구동식, 고속 폭기식 및 벤튜리식 포말분리기에 의한 양어장 수의 단백질 및 부유 고형물의 제거 특성

  • 김병진;서근학;조재윤;김성구
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.284-285
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    • 2000
  • 분산된 공기 기포에 의해 고형물을 제거하는 포말분리법은 30 $\mu\textrm{m}$이하의 미세 입자와 용존 고형물을 매우 효율적이면서도 경제적으로 운전을 할 수 있고 연속 운전이 가능하며 역세척이 필요 없다는 장점을 지니고 있다((Clarke and Wilson. 1983). 현재 사용되고 있는 포말분리장치는 공기의 공급방식과 유체과 공기의 접촉방향에 따라 항류 공기구동식(counter current air driven type, CCAD), 고속 폭기식(high speed aeration type, HSA), 벤튜리식(venturi type) 등이 있으나 현재까지 우리나라에서 연구되어 오고있는 포말분리 장치는 공기구동 방식이 전부이다. (중략)

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