• Title/Summary/Keyword: 온도(temperature)

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Free Radical Scavenging Effect and Extraction Condition of Ethanol Extracts and Fractions of Wild Grape Seed (Vitis coignetiea) (머루종자의 적정 추출조건 및 추출 분획물의 Free Radical 소거능 효과)

  • Kim, Nan-Young;Kim, Young-Kuk;Bae, Ki-Ja;Choi, Jae-Ho;Moon, Jea-Hak;Park, Geun-Hyung;Oh, Deog-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.6
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    • pp.755-758
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    • 2005
  • Wild grape is a traditional medicine plant in north-eastern part of Asia and has been known to have healing properties for various illnesses. This study was to determine the optimum extraction condition and antioxidant activity of ethanol extracts of wild grape (V. coignetiae) seed. Also, organic solvent fractions of hexane, chloroform, ethyl acetate and butanol were obtained from the ethanol extract of wild grape seed at different temperatures. Total ethanol extraction yield of wild grape seed ranged from $4\%\;to\;12\%$ depending on the ethanol concentration, extraction temperature and time condition. The highest extraction yield of $11.9\%$ was obtained at $90\%$ ethanol condition for 12 hour at $70^{\circ}C$. However, the strongest free radical scavenging effect $(RC_{50})$ with $20.93\mu g/mL$ was observed in $70\%$ ethanol extract of wild grape seed extracted for 6 hour at $70^{\circ}C,\;while\;RC_{50}\;with\;40.42$\mu g/mL$ was observed in $90\%$ ethanol extract for 12 hour at $70^{\circ}C$. Antioxidant activity of ethanol extracts of wild grape seed increased as total phenol contents increased. Among each fraction obtained from organic solvents, ethyl acetate fraction was found to have the strongest $RC_{50}\;(8.6\mu g/mL)$ and 636.77 mg GAE/g phenol contents .

Effect of Hot Water Extracts of Roasted Rhus vemiciflua Stokes on Antioxidant Activity and Cytotoxicity (볶음 처리한 옻나무 열수추출물의 항산화성 및 암세포 성장억제효과)

  • Kwak, Eun-Jung;Jo, Il-Jin;Sung, Ki-Seung;Ha, Tae-Youl
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.6
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    • pp.784-789
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    • 2005
  • The inhibitory effects of hot water extracts of Rhus verniciflua Stokes pith and peel roasted at 170, 200 and $220^{\circ}C$ on lipid peroxidation, formation of DPPH free radicals and growth of four human cancer cells such as HepG2 (liver cancer), SNU-1 (stomach cancer), MCF-7 (breast cancer) and Widr (colon cancer) were examined. The antioxidant activities and growth inhibitory effects on cancer cells of hot water extracts of peel were higher than those of pith, and the activities were dose-dependent. The roasting temperature showing the highest antioxidant activities and growth inhibitory effects on cancer cells was in the range of $170\~200^{\circ}C$ The lipid peroxidation and formation of DPPH free radicals of hot water extracts of roasted pith and peel were inhibited to 50.9, $56.5\%\;and\;79.0,\;78.4\%$ at the concentration of $500\mu g/mL$, respectively. The growth inhibitory effects of roasted pith and peel on cancer cells were in the order of Widr (41.5, $36.0\%$) > HepG2 (61.5, $44.0\%$) > MCF-7 (92.0, $69.2\%$)> SNU-1 (100, $100\%$) cells at the concentration of $1,000\mu g/mL$ as compared with the control, respectively. These results suggest that roasted Rhus verniciflua Stokes could be an useful natural medicinal plant for colon cancer.

Synthesis of Ceramic Support for Immobilization of Microorganisms Using Fly Ash (석탄회를 이용한 미생물 고정화 세라믹 담체 제조)

  • Shin, Dae-Yong;Han, Sang-Mok;Choi, Shin-Geon
    • Journal of the Korean Ceramic Society
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    • v.39 no.9
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    • pp.857-862
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    • 2002
  • Porous ceramic supports with immobilized microorganisms for the water purifier were synthesized by firing green compacts of mixed powder comprising of fly ash, bentonite and an additive of yeast powder at 800∼1,000$^{\circ}C$ for 1h and the pore and mechanical properties of specimens were investigated. The compressive strength was increased in FB (Fly Ash + Bentonite) specimens while pore properties was decreased with increasing the bentonite content and sintering temperature. The compressive strength, bulk density, apparent density, porosity, mean pore size, pore volume and specific surface area of FB specimens at 800∼1,000$^{\circ}C$ were 89.6∼128.9 kgf/$cm^2$, 1.25∼1.43, 1.61∼1.78, 27.2∼62.2%, 7.9∼25.6 ${\mu}m$, 8.9∼$22.2{\times}10^{-5}\;cm^3/g$ and 35.2∼134.3 $m^2/g$, respectively. The pore properties of FBY (FB+yeast powder) specimens were superior to that of FB specimens, however compressive strength was decreased with increasing yeast powder content. The overall properties of 9F1B1Y (9F1B+10% of yeast powder) specimens at 900$^{\circ}C$ for 1 h were 98.7 kgf/$cm^2$, 1.20, 1.67, 68.1%, 48.9 ${\mu}m$, $29.5{\times}10^{-5}\;cm^3/g$ and 152.2 $m^2/g$, respectively. In this study, it was revealed that 9F1B1Y specimen demonstrated better S. saprophyticus adherence properties n their surface pores. Consequently, the microorganisms immobilized on porous ceramic supports showed better water purifying performance with many pores and adequate strength.

Growth Response of Lettuce to Various Levels of EC and Light Intensity in Plant Factory (배양액 농도와 광도가 식물공장에서 재배되는 적축면 상추의 생장에 미치는 영향)

  • Cha, Mi Kyung;Kim, Ju-Sung;Cho, Young Yeol
    • Journal of Bio-Environment Control
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    • v.21 no.4
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    • pp.305-311
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    • 2012
  • To investigate the influence electrical conductivity (EC) of nutrient solution and light intensity on growth of red leafy lettuce, fresh and dry weights, number of leave, chlorophyll concentration and production efficiency were evaluated through nutrient film technique system. The levels of EC were 0.5, 1.0, 1.5, 2.0, 3.0, and $6.0dS{\cdot}m^{-1}$, and those of light intensity were 120, 150, and $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Under photoperiod of 16 h/day, the temperature was maintained in the range of $20{\sim}25^{\circ}C$. Planting density was $10{\times}10cm$ (100 plants/$m^2$). When red leafy lettuce were grown in the EC range of $0.5{\sim}1.5dS{\cdot}m^{-1}$, the fresh and dry weights decreased as the EC levels and light intensity were lowered, however, Hunter's a value showed no significant differences among the treatments of EC and light intensity levels (Ex. 1). The fresh and dry weights and production efficiency ($g{\cdot}FW/kw$) were the highest in the treatment of $3.0dS{\cdot}m^{-1}$ and $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ when crops were grown under the EC range of EC $1.5{\sim}6.0dS{\cdot}m^{-1}$ (Ex. 2). But the fresh and dry weights, number of leaves, and production efficiency of $2.0dS{\cdot}m^{-1}$ were the highest when the light intensity was $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ (Ex. 3). The SPAD value increased gradually as EC levels were elevated. From the above results, we concluded that optimum levels of EC and light intensity were $2.0dS{\cdot}m^{-1}$ and $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively, for production as well as production efficiency of red leaf lettuce in plant factory.

Changes in Physio-Chemical Properties of Deer Meat Cold Storage at 4°C and -2°C (냉장저장중 사슴고기의 이화화적 성질의 변화)

  • Shin Teak-soon;Kang Han-seok;Kim Seon-ku;Lee Kil-wang
    • Journal of Life Science
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    • v.15 no.4 s.71
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    • pp.522-527
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    • 2005
  • A total of 5 female elk dEER $(220kg\pm10kg)$ were included in a study on the changes in physicochemical properties of deer meat during storage at $4^{\circ}C\;and\;-2^{\circ}C$. The deer was exposed to normal pre-slaughter handling and put under anesthesia before slaughtered. The loin and leg cuts were deboned from the carcass after 24hrs slaughter. The samples weighing approximately 300g were packaged using wrap packaging and stored for 3, 7, 11 and 15 days at $4^{\circ}C\;and\;-2^{\circ}C$. Water-holding capacity was decreased with increasing storage days at $4^{\circ}C\;or\;-2^{\circ}C$, respectively The deer meats kept at $-2^{\circ}C$ showed lower TBARS value than the meats kept at $4^{\circ}C$, and it was possible to extend the storage period of the meats. VBN values of the meats kept at $4^{\circ}C\;and\;-2^{\circ}C$ showed as edible values after storage for 15 days, although there were no significant differences among the storage temperature. pH values of loin and leg tended to be increased with the passage of storage time, and the values of the meats kept at $-2^{\circ}C$ was lower than that at $4^{\circ}C$. The change of meat softness was remarkable at $4^{\circ}C$, and the change at $-2^{\circ}C$ was slow. Therefore, it was effective to extend the storage period when the meats were kept at $-2^{\circ}C$. Color of the meats kept at $-2^{\circ}C$ was darker than that at $4^{\circ}C$, the index of red color was higher for the meats kept at $-2^{\circ}C$, and yellow color of meats kept at $-2^{\circ}C$ was more rapidly changed with the passage of storage time.

Physiological Characteristics of Medicinal Plant Extracts for Use as Functional Materials in Seasoning Sauce for Pork Meat (돈육양념소스 소재 개발을 위한 한약재 추출물의 생리활성)

  • Lee, Shin-Ho;Kang, Kyung-Myung;Park, Hyo-Jin;Baek, Lag-Min
    • Korean Journal of Food Science and Technology
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    • v.41 no.1
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    • pp.100-105
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    • 2009
  • This study investigated the physiological characteristics of various medicinal plant extracts including Crataegi Fructus (CF), Perilla frutescens Britton var. acuta Kudo (PF), Geranium thunbergii sieb. et Zucc. (GT) and Polygonum multiflorum Thunberg (PM) for use in extending the shelf-life and improving the functional properties of seasoned pork. CF and GT showed a wide spectrum of antimicrobial activities against 5 kinds of Gram (+) bacteria and 5 kinds of Gram (-) bacteria. The antimicrobial activities of the extracts decreased as the heat treatment temperature increased between 60 and $121^{\circ}C$, but their activities remained relatively high following heat treatment. The total phenolic compound contents of the CF, PF, GT, PM extracts were 66.2, 29.0, 96.6 and 13.3 mg/g, respectively. The order of their DPPH radical scavenging activity was GT > CF > PF > PF. The nitrite scavenging activities of the CF, PF and GT extracts at 0.1% concentration were 10.5, 1.6 and 3.8%, respectively. The GT extract (99.7%) showed the highest degree of lipid rancidity inhibition compared to CF (90.3%), PF (88.9%) and PM (41.2%). After heat treatment for 15 min at $121^{\circ}C$, the DPPH radical scavenging activity of CF decreased but it increased in GT and PM. The nitrite scavenging activities of CF and GT decreased after heat treatment for 15 min at $121^{\circ}C$ but remained at relatively high levels. Also, degrees of lipid rancidity inhibition remained relatively high in PF (86.3%) and GT (99.8%) after heat treatment for 15 min at $121^{\circ}C$. These results suggest that CF and GT extracts could be used as ingredients in seasoning sauce to improve the shelf-life and functional properties of seasoned pork.

A Study on Implementation and Performance of the Power Control High Power Amplifier for Satellite Mobile Communication System (위성통신용 전력제어 고출력증폭기의 구현 및 성능평가에 관한 연구)

  • 전중성;김동일;배정철
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.4 no.1
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    • pp.77-88
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    • 2000
  • In this paper, the 3-mode variable gain high power amplifier for a transmitter of INMARSAT-B operating at L-band(1626.5-1646.5 MHz) was developed. This SSPA can amplify 42 dBm in high power mode, 38 dBm in medium power mode and 36 dBm in low power mode for INMARSAT-B. The allowable errol sets +1 dBm as the upper limit and -2 dBm as the lower limit, respectively. To simplify the fabrication process, the whole system is designed by two parts composed of a driving amplifier and a high power amplifier. The HP's MGA-64135 and Motorola's MRF-6401 were used for driving amplifier, and the ERICSSON's PTE-10114 and PTF-10021 for the high power amplifier. The SSPA was fabricated by the RP circuits, the temperature compensation circuits and 3-mode variable gain control circuits and 20 dB parallel coupled-line directional coupler in aluminum housing. In addition, the gain control method was proposed by digital attenuator for 3-mode amplifier. Then il has been experimentally verified that the gain is controlled for single tone signal as well as two tone signals. In this case, the SSPA detects the output power by 20 dB parallel coupled-line directional coupler and phase non-splitter amplifier. The realized SSPA has 41.6 dB, 37.6 dB and 33.2 dB for small signal gain within 20 MHz bandwidth, and the VSWR of input and output port is less than 1.3:1. The minimum value of the 1 dB compression point gets more than 12 dBm for 3-mode variable gain high power amplifier. A typical two tone intermodulation point has 36.5 dBc maximum which is single carrier backed off 3 dB from 1 dB compression point. The maximum output power of 43 dBm was achieved at the 1636.5 MHz. These results reveal a high power of 20 Watt, which was the design target.

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PST Member Behavior Analysis Based on Three-Dimensional Finite Element Analysis According to Load Combination and Thickness of Grouting Layer (하중조합과 충전층 두께에 따른 3차원 유한요소 해석에 의한 PST 부재의 거동 분석)

  • Seo, Hyun-Su;Kim, Jin-Sup;Kwon, Min-Ho
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.22 no.6
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    • pp.53-62
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    • 2018
  • Follofwing the accelerating speed-up of trains and rising demand for large-volume transfer capacity, not only in Korea, but also around the world, track structures for trains have been improving consistently. Precast concrete slab track (PST), a concrete structure track, was developed as a system that can fulfil new safety and economic requirements for railroad traffic. The purpose of this study is to provide the information required for the development and design of the system in the future, by analyzing the behavior of each structural member of the PST system. The stress distribution result for different combinations of appropriate loads according to the KRL-2012 train load and KRC code was analyzed by conducting a three-dimensional finite element analysis, while the result for different thicknesses of the grouting layer is also presented. Among the structural members, the largest stress took place on the grouting layer. The stress changed sensitively following the thickness and the combination of loads. When compared with a case of applying only a vertical KRL-2012 load, the stress increased by 3.3 times and 14.1 times on a concrete panel and HSB, respectively, from the starting load and temperature load. When the thickness of the grouting layer increased from 20 mm to 80 mm, the stress generated on the concrete panel decreased by 4%, while the stress increased by 24% on the grouting layer. As for the cracking condition, tension cracking was caused locally on the grouting layer. Such a result indicates that more attention should be paid to the flexure and tension behavior from horizontal loads rather than from vertical loads when developing PST systems. In addition, the safety of each structural member must be ensured by maintaining the thickness of the grouting layer at 40 mm or more.

Pharmacological Activity of Chaga Mushroom on Extraction Conditions and Immunostimulating Polysaccharide (추출조건에 따른 차가버섯 생리활성 및 면역활성 다당)

  • Baek, Gil-Hun;Jeong, Heon-Sang;Kim, Hoon;Yoon, Taek-Joon;Suh, Hyung-Joo;Yu, Kwang-Won
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.10
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    • pp.1378-1387
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    • 2012
  • To investigate the pharmacological activity of chaga mushroom (Inonotus obliquus) on extraction conditions, chaga was extracted using water (reflux at $50^{\circ}C$, decoction over $90^{\circ}C$, pressure at $121^{\circ}C$) or ethanol (reflux at 50, 70, or $90^{\circ}C$). When water extract was further fractionated into crude polysaccharide (IO-CP), yields of IO-CP (4.8~16.8%) were higher than those of ethanolic extracts (IO-E, 1.9~2.7%) at increased temperature. For antioxidant activity, crude polysaccharide (IO-CP-121) obtained by pressurized extraction showed the highest polyphenolic and flavonoid contents (35.10 mg TAE/g and 18.48 mg QE/g, respectively) as well as DPPH and ABTS free radical scavenging activities (26.08 and 27.99 mg AEAC/100 mg, respectively). Meanwhile, IO-CP-D (decoction) and IO-CP-50 (reflux) had more potent mitogenic effects (2.10- and 1.95-fold of saline control at 100 ${\mu}g/mL$) as well as intestinal immune system modulating activities (6.30- and 5.74-fold) compared to IO-CP-121, whereas ethanolic extracts showed no activity. Although no IO-CP showed cytotoxicity against RAW 264.7 cells at 0.1 mg/mL, IO-CP-121 significantly inhibited TNF-${\alpha}$ and NO production as pro-inflammatory factors in LPS-stimulated RAW 264.7 cells (29.2 and 63.5%, respectively). Ethanolic extracts also showed no cytotoxicity at 0.1 mg/mL, whereas inhibition of TNF-${\alpha}$ and NO production was significantly low compared to that of IO-CP-121. In addition, active IO-CP-D was further fractionated into an unadsorbed (IO-CP-I) and seven adsorbed fractions (IO-CP-II~VIII) by DEAE-Sepharose CL-6B column chromatography in order to isolate immunostimulating polysaccharide. IO-CP-II showed the most potent mitogenic effect and macrophage stimulating activity (4.51- and 1.64-fold, respectively). IO-CP-II mainly contained neutral sugars (61.86%) in addition to a small amount of uronic acid (2.96%), and component sugar analysis showed that IO-CP-II consisted mainly of Glc, Gal, and Man (molar ratio of 1.00:0.55:0.31). Therefore, extraction conditions affect the physiological activity of chaga, and immunostimulating polysaccharide fractionated from chaga by decoction is composed mainly of neutral sugars.

Characteristics of Shear Strength and Elastic Waves in Artificially Frozen Specimens using Triaxial Compression Tests (삼축압축실험을 이용한 인공동결시료의 강도평가 및 탄성파 특성변화)

  • Kim, JongChan;Lee, Jong-Sub;Hong, Seung-Seo;Lee, Changho
    • The Journal of Engineering Geology
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    • v.24 no.1
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    • pp.111-122
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    • 2014
  • For accurate laboratory evaluations of soil deposits, it is essential that the samples are undisturbed. An artificial ground-freezing system is the one of the most effective methods for obtaining undisturbed samples from sand deposits. The objective of this study is to estimate the shear strengths and the characteristics of elastic waves of frozen-thawed and unfrozen specimens through the undrained triaxial compression test. For the experiments, Jumunjin standard sands are used to prepare frozen and unfrozen specimens with similar relative densities (60% and 80%). The water pluviation method is used to simulate the fully saturated condition under the groundwater table. When thawing the frozen specimens, the temperature is measured every minute. After the specimens are completely thawed, undrained triaxial compression tests are conducted using the same procedures as for the unfrozen specimens. During the triaxial tests (saturation, consolidation, and shear phase), compressional and shear waves are measured. The results show that the freeze-thaw process has minor effects on the peak deviatoric stress and shear strength values, and that the process does not affect the internal friction angle. The compressional wave velocity increases with increasing B-value to 1800 m/s in the saturation phase, but tends to remain constant in the process of consolidation and shearing. The shear wave velocity decreases with increasing B-value in the process of saturation, but changes velocity in accordance with the change in effective stress in the processes of consolidation and shearing. The compressional wave velocity has similar values regardless of the freeze-thaw process, but values of shear wave velocity are slighly lower in frozen-thawed specimens than in unfrozen specimens. This study is a preliminary experiment for estimating the shear strength and characteristics of elastic wave velocity in undisturbed frozen specimens that have been obtained using the artificial ground-freezing method.