• Korean Journal of Microbiology
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• v.25 no.3
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• pp.244-248
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• 1987

Attempts were made to enumerate the number of Gram positive and negative bacteria in the development of natural fermentation rapidly and simultaneously. A general Gram stain was applied to this study. The number of cells by Gram stain was proportional to the cell turbidity by spectrophotometer within a range of 0.7 absorbance at 610nm. The cells washed out during procedures were not exceeded about 8 percentage. The standard error of separate counts in the mixture of Cscherichia coli and Micrococcus luteus was $5.1\pm2.3$%. The possible range of counting was $5.5\times 10^{7}-1.0\times 10^{9}$ cells/ml. Therefore, it is believed that a general Gram stain could be applied to the separate counting of mixture of Fram positive and negative bacterial populations too. In practice, growth kinetics of hemp retting and Kimchi fermentation were presented.

• The Journal of Engineering Geology
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• v.18 no.4
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• pp.381-391
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• 2008

The eigenvalue technique is introduced to overcome the problem of truncation errors caused by temporal discretization of numerical analysis. The eigenvalue technique is different from simulation in that only the space is discretized. The spatially discretized equation is diagonized and the linear dynamic system is then decoupled. The time integration can be done independently and continuously for any nodal point at any time. The results of eigenvalue technique are compared with the exact solution and FEM numerical solution. The eigenvalue technique is more efficient than the FEM to the computation time and the computer storage in the same conditions. This technique is applied to the solute transport analysis in nonuniform flow fields around underground storage caverns. This method can be very useful for time consuming simulations. So, a sensitivity analysis is carried out by using this method to analyze the safety of caverns from nearly located contaminant sources. According to the simulations, the reaching time from source to the nearest cavern may takes 50 years with longitudinal dispersivity of 50 m and transversal dispersivity of 5 m, respectively.

• Analytical Science and Technology
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• v.18 no.4
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• pp.329-337
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• 2005

TL dating using pre-dose effect is concerned with the sensitivity increase of $110^{\circ}C$ TL peak in quartz following the pre-exposure of irradiation (pre-dose) in antiquity and heating to thermal activation temperature. Using the multiple activation (MA) method for determining an equivalent dose, the activation temperature was selected to $500^{\circ}C$ and the region of interest was selected from $85^{\circ}C$ to $105^{\circ}C$ on quartz sample from archaeological remains, roof-tile kiln. which was excavated at Chudong-Ri cultural site. It was concluded that the resultant date was determined to the age of $1725{\pm}25years$ AD (l ${\sigma}$ SD), which had more improved precision considering with e radiocarbon dates and was in good agreement with the age derived by archaeological assessment. Also it was concluded that the pre-dose dating should contribute significantly to future archaeological dating work in the range of the last 2000 years.

• Applied Chemistry for Engineering
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• v.23 no.6
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• pp.577-582
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• 2012

In this work, vibrational circular dichroism (VCD) technique was applied for the determination of %EE of chiral compounds. It may provide an easy way to determine the %EE with a proper accuracy within 2% error ranges as well as the absolute configuration of enantiomers. We demonstrated herein a flow cell VCD (FT-VCD) technique for time-dependent %EE measurements. The simultaneous monitoring of the mole fraction and %EE for two chiral species (epichlorohydrin and glycidol mixture) in the mixture was shown to be successful without any further separation steps. Thus, we demonstrate that FT-VCD is an appropriate analytical tool to monitor the kinetics of reactions involving chiral molecules. FT-VCD also provides a convenient nondestructive approach for the time dependent determination of the optical purity of individual components in a reaction mixture containing chiral molecules.

• Analytical Science and Technology
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• v.21 no.6
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• pp.502-509
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• 2008

An ID LC/MS (isotope dilution liquid chromatography/mass spectrometry) was used as a primary method for the quantitative analysis of cholesterol in human serum. The separation of cholesterol was carried out by Thermo ODS hypersil $C^{18}$ column. The mobile phase was 100% methanol, and flow rate was $0.3m{\ell}/min$. Cholesterol and cholesterol-$3,4-13C_2$ were monitored at m/z 369.4 and 371.3, which correspond to $[M-H_2O+H]^+$ respectively. In order to verify the measurement method, NIST SRM 909b was analyzed. The results agreed well with certified values within uncertainty. The four kinds of serum certified reference material were prepared and certified. The repeatabilities of measurement were ranged from 0.1 to 0.8% (RSD), which were relatively good. The reproducibility between independent measurement run was below 0.24% (RSD). The expanded uncertainty was about 1.43% within the 95% confidence interval.

• Composites Research
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• v.16 no.3
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• pp.9-17
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• 2003

In order to simulate the crack connection between cords and the interply crack growth in the belt-layer of real tire, 2 ply rubber/cord laminate specimens with exposed edges were tested in 4~11mm displacement control. Measurement of the crack connection is evaluated when crack reaches the half of the length between 45$^{\circ}$ aligned cords, and the amount of the crack growth is measured by the steel probe method. 2 dimensional analytic modeling was performed to simulate the crack connection between cords at the exposed edges. Also, the theoretical life of the specimens was calculated from the crack connection life between cords(critical value) and from the critical value to the final failure by the use of Tearing energy(T); the strain energy release per unit area of one fracture surface of a crack. Then, theoretical life was compared with those of experiments. The life prediction up to the critical value has about 20% error compared to experimental life, and up to the final failure about 65% error. Therefore, total theoretical life has about 45% error compared to the experimental life, which is conceivable in the case of rubber.

• Journal of the Korean GEO-environmental Society
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• v.11 no.9
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• pp.15-25
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• 2010

The main objective of this study was to offer informations about long-term seepage behavioral characteristics and to find a leakage safety management method for Juam Dam and Imha Dam, a central cored rockfill dams in Korea by the evaluating the automatically monitored leakage data. In the water leakage monitoring of fill dam, the generation of abnormal water leakage is difficult to directly detect due to the effect of outside factors such as the component of rainfall inherent in the observation value. Therefore, conventionally estimation methods of water leakage quantity were applied by multiple regression analysis considering reservoir water level, rainfall, etc.. However, the estimated error of rainfall component is relatively big in these method. This paper identifies the seepage characteristic of each dams which is not directly affected by rainfall through the hydrograph separation analysis and 3 dimensional analytical method, and thinks a leakage management method. It was noticed that two dams had site specific seepage behaviour features and were in stable state with the decreasing leakage quantity. It was also found that hydrograph separation method might be applicable to leakage safety management method.

• Journal of the Korean Chemical Society
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• v.27 no.4
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• pp.279-286
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• 1983

An apparatus was made for the electrodeposition of alpha emitting actinide nuclides, $^{207}Bi$ and $^{210}Po$. The electrodeposition was made on a polished stainless steel plate cathode. The anode was made of platinum wire and to stir the solution. With the ammonium chloride as electrolyte initial pH = 4, chloride concentration = 0.6M and solution volume = 15ml, a current of 1.5 ampere(current density = 0.59A/$cm^2$) was flowed for 100 minutes for the quantitative recovery of electrodeposition and on average recovery of 98.3% was obtained within ${\pm}$0.7% uncertainty. Alpha spectrometry of the electrodeposited sample showed alpha peaks from $^{210}Po, ^{234}U$ and $^{239}Pu$ having energy resolution (FWHM) of 18.3, 21.8 and 36.0 keV respectively. The electrodeposition and alpha spectrometry for a natural uranium sample of domestic origin gave $^{238}U : ^{234}U = 1 : 6.1{\times}10^{-5}$ and for a neutron-irradiated uranium sample did $^{238}U : ^{239}Pu : ^{241}Am = 100 : 0.0263 : 5.20{times}10^{-5}$. The result of $^{238}U$ determination in the irradiated sample by electrodeposition-alpha spectrometry was in accord within ${\pm}1.6%$ of relative error with the results of solid fluorimetry and mass spectrometry. For $^{239}Pu$ the result of electrodeposition-alpha spectrometry was in accord within ${\pm}$4.0% of relative error with the results of anion exchange separation and the thenoyltrifluoroacetone(TTA) extraction both followed by alpha spectrometries.

• Journal of Wetlands Research
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• v.16 no.2
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• pp.161-171
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• 2014

Recession of hydrograph gives a significant contribution to estimation of baseflow using rainfall-runoff models and baseflow separation methods, because recession affects baseflow. This study attempted to enhance the accuracy of streamflow predictions using a Soil and Water Assessment Tool (SWAT) model and to separate baseflow from the predicted streamflow. For this, this study used two scenarios: 1) to calibrate eleven parameters using an auto-calibration tool with the alpha factor obtained from RECESS (S1); and 2) to calibrate twelve SWAT parameters including alpha factor (one of SWAT parameters) using an auto-calibration tool (S2). Then, baseflow spearation from the predicted streamflow was conducted by using Web-based Hydrograph Analysis Tool (WHAT). The results show that there is no significant difference between Nash-Sutcliffe efficiency (NSE) values of S1 and S2 for calibrations to streamflow. However, calibrations to baseflow showed that NSEs are 0.777 for S1 and 0.844 for S2, which means a significant difference. Quantitatively compared to the observed streamflow, relative errors were 20.78 % for S1 and 6.59 % for S2. Finally, this study showed the importance of recession in baseflow separated from the predicted streamflow using a rainfall-runoff model.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.9 no.5
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• pp.678-686
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• 1998

This paper describes an anisotropic perfectly matched layer (APML) for mesh truncation of the Finite Difference Time Domain(FDTD) method. The APML method can classified by a split type and an unsplit type, in case of the split type be made up 12 equations or 8 equations largely, and in case of the unsplit type be made of 6 equations. Therefore the latter is more simple as compare with the former. For the APML method presented in this paper is the latter, is directly derived from the time domain equations of Maxwell and extend to the three dimensional problem for the method suggested by Chen. Especially, in the edge and corner parts except the planes, the APML method effectively treated as compound with the Chen's method and Gedney's method newly. The results of the numerical method in this paper show the radiation patterns and the time responses of electromagnetic fields of the wire antennas according to wavelengths and the APML results are compared with Mur's first order absorbing boundary condition and Kraus's analytical results. Eventually, Mur's first order condition have many errors at the edge and corner. On the other hand, in comparison with Kraus's analytical results, it is quite good agreement, and the validity of present method is confirmed.