• Journal of Dairy Science and Biotechnology
• /
• v.30 no.2
• /
• pp.75-81
• /
• 2012

Molds cause severe cheese deterioration, even though some white and blue molds are used for the manufacture of Camembert and Blue cheese, respectively. The species of Geotrichum, Moniliella, Aspergillus, Penicillium, Mucor, Fusarium, Phoma, and Cladosporium are the main fungi that affect contamination during cheese ripening. Once deteriorated by fungal spoilage, cheese becomes toxic and inedible. Fungal deterioration of cheese decreases the nutritional value, flavor profiles, physicochemical and organoleptic properties, and increases toxicity and infectious disease. Fungal contamination during cheese ripening is highly damaging to cheese production in Korean farmstead milk processing companies. Therefore, these companies hesitate to develop natural and ripened cheese varieties. This article discusses the recent and ongoing developments in the removal techniques of fungal contamination during cheese ripening. There are 2 categories of antifungal agents: chemical and natural. Major chemical agents are preservatives (propionic acid, sodium propionate, and calcium propionate) and ethanol. Among the natural agents, grapefruit seed extract, phytoncide, essential oils, and garlic have been investigated as natural antifungal agents. Additionally, some studies have shown that antibiotics such as natamycin and Delvocid$^{(R)}$, have antifungal activities for cheese contaminated with fungi. Microbial resources such as probiotic lactic acid bacteria, Propionibacterium, lactic acid bacteria from Kimchi, and bacteriocin are well known as antifungal agents. In addition, ozonization treatment has been reported to inhibit the growth activity of cheese-contaminating fungi.

• Korean journal of applied entomology
• /
• v.27 no.1
• /
• pp.35-40
• /
• 1988

The control efficiency of the nematicides, aldicarb and carbofuran, in the population dynamics of the soybean cyst nematode(Heterodera glycines)was evaluated. Nematode viability, penetration and reproduction were examined in greenhouse experiments. Nematode viability(movement) was greatly reduced by aldicard, but mot by carbofuran. Penetration was inhibited by both chemicals. Percentages of Pentration relative to the untreated check 3 days after treatment were 0.4% and 1.7% for aldicarb and carbofuran, respectively. Systemic effect ot the nematicides were observed with both nematicides but effect was greater with aldicarb than with carbofuran. The total effect of the nematicides, applied ay the time of planting soybean in infested soil, appeared to be less than the sum of the contact and systemic effects on the initial population dynamics of H. glycines.

• Journal of Korean Society on Water Environment
• /
• v.32 no.2
• /
• pp.205-215
• /
• 2016

In order to improve water quality in the Paldang Lake, a riparian vegetation belt, treatment wetland, and artificial floating island were designed for introduction in the upland field, the estuary of tributaries, and the section of water facing mountainous land, respectively. We synthesized vegetation information collected from a reference river and found that herbaceous, shrubby, and tree vegetation zones tended to be dominated by Phragmites japonica, Phalaris arundinacea, etc.; Salix gracilistyla, S. integra, etc.; and S. koreensis, S. subfragilis, and Morus alba, respectively. In our plan, the herbaceous vegetation zone, which is established on floodplains with a high frequency of disturbance, will be left in its natural state. A shrubby vegetation zone will be created by imitating the species composition of the reference river in the ecotone between floodplain and embankment. A tree vegetation zone will be created by imitating species composition on the embankment slope. In the treatment wetland, we plan to create emerged and softwood plant zones by imitating the species composition of the Zizania latifolia community, the Typha orientalis community, the P. communis community, the S. integra community, and the S. koreensis community. The floating island will be created by restoring Z. latifolia and T. orientalis for water purification purposes.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2017.05a
• /
• pp.102-102
• /
• 2017

갈수시 하천의 수질문제는 다양한 요인에 의해 발생하고 제어된다. 수질 그 자체는 상류나 지류의 점오염원과 비점오염원의 증가 또는 예상치 못한 추가적인 오염원의 유출에 직접 영향을 받으며, 조류 등의 발생도 영향인자인 수온 및 일사량 또는 바람에 의존적이다. 최근 수년간은 특히 빈번한 가뭄조건의 발생으로 인하여 갈수기의 강우 및 유출량이 저조하여 상대적으로 하천의 조류발생이 다수 발견되고 보고된 바 있다. 이와 같은 수질의 이상상태를 일시적으로라도 제어하기 위한 방편으로 유량의 증가, 인위적인 하천 수위의 변동 및 유속 변동 등이 고려될 수 있으며, 이를 위해서는 상류의 댐과 하천의 다기능보 등의 조절을 탄력적으로 수행하여야 한다. 이와 같은 시설물 운영효과는 수질문제의 원인을 근본적으로 해결하는 것은 아니지만, 이의 일시적인 효과를 극대화하기 위해서는 기존의 수리 수질 모델링이 이상 수질 발생시의 방제 및 사후분석 등을 중심으로 이루어진 부분을 넘어서, 사전 설정된 조건에 의한 예측모의와 가이드라인의 연계방식이 효과적일 것이다. 본 연구는 수질 이상이 빈번히 발생되었던 낙동강의 칠곡 하류 하천을 중심으로 2차원 CE-QUAL-W2 모형과 EFDC모형을 병행 모의하여 다기능보 인근의 표층과 저층의 수온(밀도) 성층화 및 이의 해소와 관련된 수리모의 및 수질인자에 대한 영향을 분석하였다. 수리모형 구축의 적정성은 현장의 실측 수온과 모의결과 비교를 통해 확인하였으며, 구축된 수리 수질 모델을 이용하여 추가방류량 3~23백만$m^3$ 규모에서 발생하는 수리적인 수층혼합 현상과 일시적으로 저감가능한 수질개선 효과가 의미있는 수준으로 나타날 수 있음을 예측하였다. 또한, 다기능보의 수문방류 모의시에 개방조건을 0.3~3.5m로 달리하여 방류기간 중 총방류량이 유사하더라도 최대방류량 또는 유속이 충분치 않은 경우에는 혼합효과가 급격히 감소함을 알 수 있었다. 연구에서 밝혀진 조건들을 향후 보다 효과적으로 수치모의하기 위한 방안으로서 EFDC모형의 내부경계조건을 다기능보의 문비조건(RSG, Lift 등)에 맞게 조정하는 방법을 제안하고 그 적용성을 검토한 뒤 모형을 부분 수정하여 제시하였다.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.30 no.2C
• /
• pp.119-131
• /
• 2010

In this study, beneficial use of ocean contaminated sediments were investigated by laboratory and environmental tests, and their prototypes were released. Dredged material from Ulsan port is used for making cement treated samples and lightweight foamed samples, and various engineering tests were performed to identify the compressibility and stress-strain behaviors. Environmental tests were also performed for the beneficial uses. The values of Cu are a little higher than the suggested standard possible for reusing dredged material and equal to the suggested standard alarming for reusing dredged material, which shows environmental harmfulness for the reuse of construction material. In addition, particle size distribution, compaction test, Atterberg limit tests, specific gravity test, and unit weight test were performed to investigate the use of landfill cover materials. The shear strengths of cement treated soils were found to be enough for reclamation works.

• Korean Journal of Microbiology
• /
• v.49 no.1
• /
• pp.64-70
• /
• 2013

The greenhouse whitefly, Trialeurodes vaporariorum, is an economically important pest for greenhouse crops because they cause direct damage by feeding on plant nutrients and indirect damage as transmits many virus vectors. It has recently become a serious problem because of the continuous use of insecticide resulting in resistance among greenhouse whitefly population. To overcome these problems, in this study, the biological characteristics and virulence of an entomopathogenic fungus isolated from the cadaver of nymph greenhouse whitefly were investigated. Isolated fungus was identified as Isaria fumosorosea by morphological examinations and genetic identification using sequences of the ITS, ${\beta}$-tubulin, and EF1-${\alpha}$ regions. This fungus was named as I. fumosorosea SDTv and tested for the virulence against nymphs T. vaporariorum and the cold activity, the thermotolerance and the stability of UV-B irradiation on conidia. Mortality rate of greenhouse whitefly showed from 84 to 100% and the virulence increased with increasing conidial concentrations, $1{\times}10^5$ to $10^8$ conidia/ml. Conidia were stable at $35^{\circ}C$, 0.1 $J/cm^2$ of UV irradiation and germinated after 8 days at $4^{\circ}C$. Additionally, the activities of chitinases and proteases produced by I. fumosorosea SDTv were varied according to the medium. In conclusion, I. fumosorosea SDTv which showed high mortality rate against greenhouse whitefly will be used effectively in the integrated pest management programs against the greenhouse whitefly.

• Research in Plant Disease
• /
• v.22 no.4
• /
• pp.227-235
• /
• 2016

Plant disease caused by root-knot nematode is a major problem in crop production. Using of chemical pesticides, one of the most efficient methods to control nematodes, have raised issues in toxicity to humans and animals and environmental pollution. In this study, to select actinomycete strains that have potential to serve as a microbial agent for control of nematodes, we investigated nematicidal activity of culture broth from 670 Streptomyces isolates. A culture filtrate of KRA15-528 isolate that was identified as S. flavogriseus on the basis of 16S rRNA sequence analysis, showed strong nematicidal activity against second stage of juveniles of Meloidogyne incognita and inhibited egg hatching; exposure to 10% of culture filtrate resulted in 71% juvenile mortality at 48 hours afters treatment and suppressed egg hatching by 54% at 9 days after treatment. When the KRA15-528 culture filtrate was partitioned with ethyl acetate and butanol, ethyl acetate layer exclusively showed strong activity; 91%, 53%, 30% of mortality at 1,000, 500, $250{\mu}g/ml$, respectively. Additionally, the culture filtrate suppressed gall formation on cucumber plant by M. incognita with no phytotoxicity. These results suggest that S. flavogriseus KRA15-528 has potential to serve as a microbial nematicide for the control of root-knot nematode disease.

• The Korean Journal of Pesticide Science
• /
• v.16 no.4
• /
• pp.315-321
• /
• 2012

Fate of acetamiprid and imidacloprid aerially sprayed to control pine wood nematode (Bursaphelenchus xylophilus) were studied in a forest of Haman area. Acetamiprid 20% SL or imidacloprid 20% DC were diluted 100 times and applied two times as rate of 50 L/ha using an aircraft of Bell 206 L helicopter. Average acetamiprid deposits on forest floor ranged from 2 to 4% of standard aerial application rate. Following to the second application, acetamiprid deposits in the pine needle ranged 1.8~8.5 mg/kg and then gradually decreased to 1.2~2.1 mg/kg after 48 days. Deposits on the plant washed off by rainfall and reached to soil surface was ca. 17% of the application rate. All of acetamiprid on the ground resided in the forest floor covering the soil surface, where acetamiprid residues were decreased to a quarter at 48 days after the second application, but they were not detected in soil beneath it. And the only low level of acetamiprid residues, 0.0003 mg/L, was detected in the reservoir nearby the experimental forest on the day of aerial application. The acetamiprid detection was presumably due to spray drift. And average imidacloprid deposits on forest floor ranged from 1 to 3% of standard aerial application rate. Following to the second application, imidacloprid deposits in the pine needle analysed very low concentration of 0.1 mg/kg, but the amount of imidacloprid in wash-off in standard and two-fold treatment were ca. 8% and 4% of the application rate, respectively. Most of imidacloprid on the ground also resided in the forest floor, where imidacloprid residues were decreased to a twentieth at 111 days after the second application, and they were detected below 0.5% of the application rate in sol beneath it. And the low level of imidacloprid, 0.0003~0.0017 mg/L, were detected in the streams in the experimental forest. It was not to the level of contamination concerns.

• Research in Plant Disease
• /
• v.21 no.2
• /
• pp.50-57
• /
• 2015

Control of nematode has become difficult owing to the restricted use of effective soil fumigant, methyl bromide, and other non-fumigant nematicides. Therefore, it is urgently necessary to develop microbial nematicide to replace chemical nematicides. In this study, the 50% aqueous methanol extraction solution of fermentation broths of 2,700 actinomycete strains were tested for their nematicidal activity against second stage of juveniles (J2s) of Meloidogyne incognita. As the results, only the 50% aqueous methanol extraction solution of AN110065, at 20% equivalent to 10% fermentation broth, showed strong nematicidal activity with 78.9% of mortality 24 h after treatment and 94.1% of mortality at 72 h. The 16S rRNA gene sequencing showed that the strain sequence was 99.78% identical to Streptomyces netropsis. The extract of S. netropsis AN110065 fermentation broth was successively partitioned with ethyl acetate and butanol and then the ethyl acetate, butanol and water layers were investigated for their nematicidal activity against the M. incognita. At $1000{\mu}g/ml$, ethyl acetate layer showed the strongest activity of 83.5% of juvenile mortality 72 h after treatment. The pot experiment using the fermentation broth of AN110065 on tomato plant against M. incognita displayed that it evidently suppressed gall formation at a 10-fold diluent treatment. The tomato plants treated with the fermentation broth of S. netropsis AN110065 did not show any phytotoxicity. The results suggest that S. netropsis AN110065 has a potential to serve as microbial nematicide in organic agriculture.

• Research in Plant Disease
• /
• v.30 no.1
• /
• pp.66-77
• /
• 2024

Black wood ear mushroom (Auricularia auricula-judae) is one of the most economically important mushrooms in China, Japan, and Korea. The cultivation of wood ear mushrooms on artificial substrates is more efficient in terms of time and cost compared with their natural growth on trees. However, if the substrate cultivation is infected by fast-growing fungi, the relatively slow-growing ear mushroom will be outcompeted, leading to economic losses. In this study, we investigated the competitive fungal isolates from substrates infected with fast-growing fungi for the cultivation of ear mushrooms in Jangheung and Sunchon, Korea. We collected 54 isolates and identified them by sequencing their internal transcribed spacer region with morphological identification. Among the isolates, the dominant isolates were Trichoderma spp. (92.6%), Penicillium spp. (5.6%), and Talaromyces sp. (1.8%). To find an appropriate eco-friendly biocontrol agent, we used five Streptomyces spp. and Benomyl, as controls against Trichoderma spp. and Penicillium spp. Among the six Streptomyces spp., Streptomyces sp. JC203-3 effectively controlled the fungi Trichoderma spp. and Penicillium spp., which pose a significant problem for the substrates of black wood ear mushrooms. This result indicated that this Streptomyces sp. JC203-3 can be used as biocontrol agents to protect against Trichoderma and Penicillium spp.