• Analytical Science and Technology
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• v.29 no.2
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• pp.94-103
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• 2016

An analytical method was developed to determine the amount of spinetoram (spinetoram J and spinetoram L) in livestock samples. The spinetoram was extracted with acetonitrile and purified through a primary secondary amine (PSA) sorbent. The spinetoram residues were then quantified and confirmed using a liquid chromatography–tandem mass spectrometer (LC-MS/MS) in the positive ion mode using multiple reactions monitoring (MRM). Matrix-matched calibration curves were linear over the calibration ranges (0.005-0.5 mg/kg) into a blank extract with r² > 0.994. The limits of detection and quantification were 0.002 and 0.01 mg/kg, respectively. The recovery results of spinetram ranged between 81.9-106.4% at different concentration levels (LOQ, 10LOQ, 50LOQ, n=5) with relative standard deviations (RSDs) less than 10%. All values were consistent with the criteria ranges requested in the Codex guidelines (CAC/GL40, 2003). An interlaboratory study was conducted to validate the method. The proposed analytical method proved to be accurate, effective, and sensitive for spinetoram determination. The method will be used as an official analytical method in Korea.

• Journal of Food Hygiene and Safety
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• v.28 no.1
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• pp.19-23
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• 2013

A simple and rapid method has been developed and validated for simultaneous determination of each macrolides residues (spiramycin, josamycin, tilmicosin, tylosin) in chicken muscle by high-performance liquid chromatography- photo diode array (HPLC-PDA). Chicken muscle sample have been extracted with liquid-liquid extraction process; analytes was extracted by acetonitrile, and then defatted with hexane saturated by acetonitrile. The HPLC separation was performed on a Unison UK-$C_{18}$ ($150mm{\times}3.0mm$, $3{\mu}m$) with a gradient system of 0.1% trifloroacetic acid (TFA) and 0.1% trifloroacetic acid (TFA) in acetonitrile as the mobile phase. The drugs were detected at 232 nm for spiramycin and josamycin, and 287 nm for tilmicosin and tylosin. The limits of quantification (LOQs) were between 27 and $59{\mu}g/kg$; and the intra- and inter-day precision (relative standard deviation; RSD) was between 0.9-13.2 and 2.4-13.1%, respectively in chicken muscle sample. The method may has been successfully applied for multiresidue determination of four macrolides below the maximum residue limits (MRLs) established by the European Union (EU).

• Journal of Korean Society of Environmental Engineers
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• v.22 no.5
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• pp.981-987
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• 2000

Upflow anaerobic sludge blanket(UASB) reactor was operated for treating swine wastewater containing high ammonia nitrogen to assess their performance and toxicity of free ammonia concentration. In the reactor, chemical oxygen demand(COD) removed about 70% at $2.6kgCOD/m^3.day$ of organic loading rate(OLR) and 3 days of hydraulic retention time (HRT), while it was decreased when OLR and HRT was maintained $7kg\;COD/m^3.day$ and 2 days, respectively. Also UASB reactor was evaluated the activity of methane producing bacteria(MPB) according to change of free ammonia concentrations, MPB activity of applied sludge in the 500 and $1000mg-N/{\ell}$ of free ammonia concentration was inhibited by 4% and 40%, respectively. This clearly showed that free ammonia concentration less than $500mg-N/{\ell}$ showed no inhibition to MPB in anaerobic treatment of organics, UASB reactor was stabilized easily less than $1000mgVSS/{\ell}$ due to degradation of organic solids by the high activities of anaerobes.

• Food Science of Animal Resources
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• v.29 no.5
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• pp.640-646
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• 2009

Lactic acid bacteria (LAB) are typical probiotic microbes that are used in various industries including fermented foods, feed additives, and pharmaceuticals. The purpose of this study was to compare the ability of isoflavone biotransformation and antioxidative activity of 17 LAB. Six strains including the Lactobacillus species exhibited a 100% hydrolysis rate for daidzein during fermentation. The content of total genistein in soymilk fermented with these strains was $872-943\;{\mu}g/g$. The DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging ability of the LAB was widely variable and ranged from 23-78%. A selected strain was isolated from kimchi and the strain was identified as Lactobacillus plantarum ssp. through the API carbohydrate fermentation pattern and 16S rDNA profile. The strain exhibited excellent acid tolerance in an artificial gastric solution. L. plantarum YS712 showed high $\beta$-glucosidase activity in fermentation. The concentration of genistein and daidzein in soymilk fermented with L. plantarum YS712 increased from 3.64 to $917.3\;{\mu}g/g$ and from 58.18 to $1062.17\;{\mu}g/g$, respectively. These results demonstrate the potential of L. plantarum YS712 as a probiotic culture that can be utilized in the manufacturing of fermentation foods and dietary supplements.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.3
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• pp.523-533
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• 2000

A two-phase anaerobic reactor with submerged membrane system was developed for increasing acidogen concentration and methane recovery. The membrane used was mixed esters of cellulose of $0.5{\mu}m$ pore size and $0.8m^2$ of effective surface area. The methanogenic reactor comprised of UASB (Upflow Anaerobic Sludge Blanket) and AF (Anaerobic Filter). COD removal efficiency was 70~80% and the methane content in the biogas increased up to 90% for the submerged membrane system in the anaerobic reactor. As the cake resistance of membrane caused a serious problem, stainless steal prefilters (40, 53, $63{\mu}m$) and air backwashing methods were applied to minimize the cake resistance effectively. Among the tested prefilters. the $63{\mu}m$ prefilter showed the best performance for reduction of cake resistance and a successful long-tern operation. By cleaning with alkali first and acidic solution later. the permeate flux decreased by long term operation was recovered to 89% of that with a new membrane.

• Journal of Agricultural Extension & Community Development
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• v.21 no.3
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• pp.243-269
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• 2014

Traditional agriculture technology is absolutely valuable not only to preserve biological diversity but construct an eco-friendly agricultural system. As the awareness of food safety has been growing, traditional technologies in the past agriculture literatures have obtained more attention sine chemical or synthesized fertilizer and agricultural pesticide were not used in these traditional agriculture ways. This study aims to evaluate development values on six kinds of agriculture literatures published before initial 1970s using Delphi technique and Correspondence analysis. The domains of assesment are include livestock sanitation, breeding management, feeding program and an extra part which 261 traditional agriculture technologies were first extracted from. From these technologies, livestock experts deducted 228 items and selected items more than 3.0 from all three areas including usefulness, scientific characteristic and economical efficiency utilizing Delphi technique.: 35 items from usefulness, 31 items from scientific characteristic, and 18 items from economical efficiency. The 23 technology items were finalized after adding five modern feasibility items recommended by experts to 18 items gaining more than 3.0 in at least three areas. The agriculture technologies were categorized into four areas where were analyzed using Correspondence analysis. The results revealed that 'sustainable farming technology', 'scientific character verification', ' modernized technology development', and 'intellectual property right' were placed closely one another except 'economy efficiency'. The result implicated that scientific verification and economy efficiency should be considered in order to sustain traditional agriculture technology into a modernized way and to protect intellectual property right of these technologies.

• Food Science of Animal Resources
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• v.25 no.4
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• pp.472-477
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• 2005

This experiment was studied to investigate the physico-chemical properties and sensory evaluation in pickled quail egg during storage at 10 and $20^{\circ}C$ for storage periods 15 days. The weight change of the pickled quail egg were saw a big increase rate with the pickle during 10 days later, and the during storage at $20^{\circ}C$ were increased than the during storage at $10^{\circ}C$. Moisture, crude protein, crude fat and crude ash content of quail egg were 75.24, 12.13, 9.53 and $1.20\%$ respectively. The pH were showed increase pattern for pickle storage periods on 5 days at $10^{\circ}C$. Water soluble nitrogen of quail egg white saw decreased with during the pickle peroids at 10 and 15 days, and the non-protein nitrogen saw increased with according to pickle periods. The change of the viscosity by a rotation viscometer measurement saw increased with according to pickle period days. and the viscosity values saw increased on pickle periods at 5 days. The result of sensory evaluation of sample was S-3>S-2>S-1, in order.

• Food Science of Animal Resources
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• v.27 no.3
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• pp.337-344
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• 2007

We have investigated the relationship between food allergen sensitization and allergic disease in 74 child (male 47, female 27) patients from 0 to 14 years of age diagnosed with allergic disease. The age distribution for the study was: newborn to 3 years old, 34 children; 4 to 6 years old, 24 children; 7 to 9 years old, 8 children and above 10 years old, 8 children. Of the 74 children, 10 children were allergic to 3 of the 21 types of foods tested, 21 children were allergic to 4 types and 15 children were allergic to 5 types. The results of specific IgE tests for class 2 (0.070-3.49 IV/mL, IgE density in serum) showed that 29 children were allergic to milk, 28 children to bean, 21 children to cheese, 7 children to egg, and 18 children to pork, while over class 2, 20 children were allergic to bean, 17 children to milk, 24 children to cheese, 20 children to egg, and 21 children to pork. A questionnaire was used to survey family allergy history and diet patterns for 40 child (male 22, female 18) patients with allergic disease. The frequencies of a family history of allergy were 45.5% for males and 50.0% for females. The allergic diseases included atopic dermatitis: 26.0%, atopic nasitis: 10.5%, atopic dermatitis + atopic nasitis : 31.5%, hives: 21.0%, and asthma: 10.5%. Children on diets of mixed breast feeding and infant formula were more allergic than those on either breast feeding or infant formula feeding. Eliminated allergenic foods were egg + milk: 12.5%, egg: 10.0%, and milk 2.5%.

• Journal of Animal Science and Technology
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• v.44 no.6
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• pp.661-668
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• 2002

This study was conducted to investigate the effect of environmental factor on milk yield and to estimate lactation curve in Korean cattle. The data for milk yields were collected from 118 cows from 1997 to 2000 at National Livestock Research Institute in Daekwanryoung, Kangwon-do. Average daily milk yields for 1st, 2nd, 3rd and 4th month after calving were 3.74kg, 3.64kg, 3.26kg and 2.99kg. Average daily milk yield for the four months was 3.52kg. The milk yields for cows calved in spring were larger than those calved in fall. Lactation curve of Korean cattle was $y_n$=$2.4845n^{0.1734}e^{－0.0060n}$. Peak milk yield was 3.75kg on 29.03 day after calving. The peak milk yields for multi-parous cows were larger than those of primiparous cows. The peak milk yields for multi-parous cows reached later than those for primiparous cows. The cows calved in spring had higher and earlier peak milk yields than those calved in fall had.

• Food Science of Animal Resources
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• v.31 no.1
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• pp.107-114
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• 2011

This study was conducted to investigate the effect of feeding whole crop barley silage (WBS) on the growth performance, carcass grade, and beef quality of Hanwoo steers. Twelve Hanwoo steers (12-mon-old) were allocated into either a control (rice straw fed) or WBS (whole crop barley silage fed) group (six animals per group) and fed for 540 d until the animals reached 30-mon-old. Concentrates and roughage were fed according to the feeding stage phases; finisher I (12.16-monold), II (17.21-mon-old), and III (22.30-mon-old). A significant increase in growth was observed during the finisher I stage for the steer fed WBS, whereas a significant increase in growth occurred during the finisher II stage in the control group. The yield grade of the WBS group increased significantly with a 15.6% increase in the longissimus dorsi area and a 25.7% reduction in back fat thickness. Marbling scores improved 52.4% in the WBS group compared with the control group. A significant difference in treatments was observed for the proximate chemical composition of the crude fat content. The results regarding palatability traits in the WBS group showed a significant improvement in appearance. The results indicate that the WBS group had improved longissimus muscle and marbling scores on carcass grading compared to the control.