• Korean Journal of Plant Taxonomy
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• v.37 no.4
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• pp.401-418
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• 2007

We performed the numerical analyses of thirty two morphological characters for twenty four populations of eight Korean Ilex L. taxa. Principal component analyses showed that the first three principal components were related to the total covariance by 67.0%, and the proportions of PCl, PC2 and PC3 were 31.5%, 21.1%, and 14.4%, respectively. And the closely related characteristics to the PCl, PC2 and PC3 were some reproductive characters such as the morphology of sepal, petal, anther, pistil and fruits and vegetative characters such as the morphology of petiol and leaf margin, the trichomes on the twigs, the leaf duration. From the two dimensional plottings by the eigenvalues of PCl, PC2 and PC3, six grouped were clustered as Ilex integra, I cornuta, I x wandoensis, I. rotunda, I. macropoda and I. macropoda for. pseudomacropoda, I. crenata and I. crenata var. microphilla. The numerical analysis was useful for the taxonomy of Korean Ilex because it clearly seperated the populations of taxa included in this study. The identification key was provided with the diagnostic characters.

• Journal of Korean Society of Forest Science
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• v.95 no.1
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• pp.55-59
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• 2006

The objectives of this study, an analysis of the morphological characteristics among six provenances of a rare Rhododendron micranthum could be used for the conservation of gene resources and could provide information on superior trees selection. The following results were obtained. Approximately Mt.worak region showed larger values at petal character. On the other hand, Bonghwa region showed smaller values at petal character. Yeonha-ri region showed larger values at leaf character. On the other hand, Bonghwa region showed smaller values at leaf character. The results of principal component analysis (PCA) for morphological characteristics showed that the first for principal components(PC's) explained 41.6% of the total variation. From th third PC explained 81.5% of the total variation. The first PC was correlated with those characteristics that were mainly related to the Petal length (PL), Leaf length (LL) width (LW), Stigma length (SL). The second PC was correlated with the Petiole length (PTW), Anther length (AL). The third PC was correlated with the Flower pedicel length (FPL), Filament length (FL). Therefore, these characteristics was important to analysis of the variation for morphological characteristics among provenances of Rhododendron micranthum. Cluster analysis using single linkage method based on morphological characteristics showed that six provenances of Rhododendron micranthum could be clustered into three groups. Group I is Jicdong-ri, Group II is Mt.worak and Yeonha-ri, and Group III is Taeback, Bonghwa, and Samcheok. These results corresponded well with that of principal component analysis.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.617-622
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• 2011

The study was conducted to determine the efficiency in producing spontaneous polyploids in some mandarin hybrids with different seed embryony. Seed formation by open pollination, frequency of spontaneous polyploids, and plant growth characteristics were evaluated in four mandarin hybrids with polyembryony such as 'Amakusa', 'Haruka', 'Hayaka', and 'Seminole' and two with monoembryony such as 'Benibae' and 'Harehime'. The mean number of the developed seeds per fruit was 10.0 and frequency of small seeds was 25.1%. Polyploids were selected from plants germinated in vitro by a flow cytometry and confirmed by chromosome analysis. One triploid was produced from 'Harehime', one tetraploid, 'Amakusa', and one tetrapoid, 'Benibae'. There were little differences in leaf shape, thickness, petiole length, and internode length between diploids and polyploids such as tri- or tetraploid. However, polyploids had larger stomata and lower density of stomata in abaxial epidermis than diploids. SPAS indicating chlorophyll content and photosynthetic rate were significantly affected by ploidy level. The results indicated that spontaneous polyploids might be produced by open pollination in some mandarin hybrids and monoembryony had higher frequency in polyploid occurrence than polyembryony.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.26-34
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• 2015

This study was conducted to examine the morphological characteristics and variations of the 6 populations of Pourthiaea. villosa (Thunb.) Decne. var. villosa in Korea. The ANOVA results showed that the populations were significantly different in 18 of the 21 quantitative characteristics that were analyzed. In the results of principal component analysis, 6 principal components (PC) represented 68.28% for the total variations. 'Petiole length' (0.764), 'Leaf shape' (0.834), 'Leaf length' (0.753), 'Crown diameter' (0.663), 'Inflorescence width' (0.492), and 'Leaf base shape' (0.721) showed the highest contribution to PC1, PC2, PC3, PC4, PC5, and PC6, respectively. According to the results of cluster analysis, one cluster comprised only 'Goesan-gun Ssanggok Valley' population. 'Namhae-gun Mijori Evergreen Forest' population was grouped with 'Hamyang-gun Sang Forest' population, which was the nearest subgroup. Further, 'Namhae-gun Geum Mountain' population, 'Jindo-gun Cheomchal Mountain' population, and 'Jeju-si Barimae Parasitic volcano' population were placed in the same cluster.

• Korean Journal of Plant Resources
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• v.15 no.3
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• pp.260-268
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• 2002

Morphological characteristics and growth patterns of 20 Polygonatum collections indigenous to Korea were examined and the collections were classified to obtain the basic data for practical use of Polygonatum genetic resources. Based on the cluster analysisi 20 collections were distinctly classified into seven groups with average distance greater than 0.6 between groups. Group I was p. sibiricum Delar, and group II included p. odoratum var. pluriforum Ohwi, P. odoratum var. pturiforum Ohwi 'Variegata' and P. odoratum var. maximowiczii Koidz.. Group III was P. odoratum var. thunbergii Hara, group IV included P. lasianthum var. coreanum Nakai, and group V was P. involucratum Maxim. and P. desoulavyi Komarov. group Ⅵ was P. inflatum Komarov and group Ⅶ was P. humile Fischer ex. Maxim. Morphologically, group I was larger than the other groups, group II and III were medium, and group IV to Ⅶ were small. In the classification of genus Polyognatum, stem length, stem habit, phyllotaxis, stem angularity, petiole, inflorescence, perianth, bract and rhizome were particularly important characters. Group I to III were thought to be useful as the edible and medicinal resources plants, and group IV to Ⅶ were thought to be useful as ornamental plants.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.5
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• pp.676-682
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• 2012

The objectives of this study focused on measuring chlorophyll fluorescence related to drought stress comparing some parameters. Almost parameters were declined although they were not significant on the basis of mean values of fluorescence of total leaf area. While the ratio of fluorescence intensity variable chlorophyll ($F_V$) to fluorescence intensity maximal chlorophyll ($F_M$) was not changed, the effective quantum yield of photochemical energy conversion in photosystemII (${\Phi}PSII$) and chlorophyll fluorescence decrease ratio ($R_{fd}$) were slightly reduced, indicating inhibition of the electron transport from quinone bind protein A ($Q_A$) to quinone bind protein B ($Q_B$). Some parameters such as non-photochemical quenching rate ($NPQ_{_-LSS}$) and coefficients of non-photochemical quenching of variable fluorescence (qN) in mid-zone of leaf and near petiole zone leaf were significantly enhanced within 4 days after drought stress, which can be used as physiological stress parameters. Decrease in ${\Phi}PSII$ could was significantly measured in all leaf zones. In conclusion, three parametric evidences for chlorophyll fluorescence responses such as ${\Phi}PSII$, NPQ, and qN insinuated the possibility of photophysiological indices under drought stress.

• Korean Journal of Plant Taxonomy
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• v.36 no.4
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• pp.263-277
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• 2006

Anemone pendulisepala, recently described as a new species, is distributed in sympatry with A. reflexa, A. amurensis, and A. raddeana at Mt. taebeark and Mt. Baekdu. Anemone pendulisepala was previously proposed to be a hybrid species between A. reflexa and A. amurensis becaue it displavs overlapping features with them in involucre shape, petiole length, sepal apex and xylem shape, To verify the taxonomic status and to examine the hybridity of A. pendulisepala, sequences of ITS region of nuclear ribosomal DNA and the psba-trnH, rps16 and trnLF region of cpDNA from 36 accessions of 5 taxa including outgroup were analyzed. In maximum parsimony tree based on ITS sequences, A. pendulisepala had the same sequences of A. reflexa and was clustered with monophyletic A. amurensis, and then A. raddeana. Anemone pendulisepala was distinguished from the other taxa by having four base insertion in rps16 region, two species-specific bases and insertion in trnLF region. In the phylogenetic trees of combined cpDNA, A. pendulisepala showed monophyly with the bootstrap 100%. Anemone pendulisepala exhibited no polymorphism and shared no sequences with putative parental or related taxa examined in this study. Molecular data suggest that A. pendulisepala should be a distinct species, and no evidence of the hybridization was detectcd.

• Korean Journal of Environmental Biology
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• v.19 no.4
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• pp.254-260
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• 2001

After investigating the developmental process of galls forming on leaf of Zelkova serrata and analyzing the composition and contents of the free amino acids in leaves, galls and haemolymph of aphids, we have come to the following results. Galls induced were mostly distributed on the middle of lower part of leaf to the petiole along vein and most of investigated leaves had one gall only. The growth of galls continued in length and width until Paracolopha morrisoni habitants which transformed into alate adult left the gall. These results also indicate that galls are closely connected with a portion of plant tissues as well as foods and habitats of aphids. 26${\sim}$29 kinds of free amino acids such as proline, alanine and valine and so on, detected in leaves, gall and haemolymph of aphids. The composition of free amino acids were similar in three materials examined, but proline and asparatic acid showed the highest quantity in the leaves and falls, respectively. In case of aphids, alanine was extremely higher as 18.4% of total contents of free amino acids, in 1st instar of fundatrix stage. Total contents of free amino acids in the apteral adult stage of aphid were also increased than in the 1st instar stage. This investigation implies that nitrogen source such as free amino acids is apparently a major growth-material like carbohydrates during development of galls in Zelkova serrata.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.488-494
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• 2010

In this study, the major agronomic traits were investigated and RAPD technique was applied for the analysis of the genetic relations between the native ginsengs collected from Poonggi and Geumsan provinces in Korea. The main morphological traits were measured for a total of 54 collections of native ginseng from two areas based on UPOV standard. A total of 58 collections consisting of twenty-one native ginsengs collections from Poonggi area, twenty-nine collections from Geumsan area and four varieties of P. quinquefolium, P. japonicum, Chunpoong and Hwangsuk as controls were analyzed and clustered by RAPD. The results indicated that 01-9, 01-35 and 01-44 collections from Poonggi area were grouped into Geumsan area, while 332001, 332002 and 332003 collections from Geumsan area were grouped into Poonggi area. On comparison to the similarity of Poonggi collections (73-95%), the Geumsan collections showed 65-86% similarity in the population. Thus, the cluster should be applied according to the number of stem, number of leaves per stem and leaflet shape on the regionally native ginseng collections. The fourteen primers such as OPA02, OPA07, OPC08, OPD11, OPD20 and so on, will be used to select the native ginseng in the future studies.

• Journal of Bio-Environment Control
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• v.32 no.1
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• pp.40-47
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• 2023

There are many different types of cultivation in tomatoes for year-round production. One of them, semi-forcing cultivation is characterized by growing seedlings in winter season. If grafted seedlings are used in winter season that energy cost can be reduced, because they have tolerance to cold stress. This study was conducted to analyze the rootstock performance by measuring the growth, yield, and leaf-macronutrient content of cherry tomatoes grown in semi-forcing hydroponics. Three domestic rootstocks 'HSF4', '21LM', '21A701', and a control cultivar 'B-blocking' were grafted onto jujube-shaped cherry tomato (Lycopersicon esculentum L.) commercial cultivar 'Nonari'. The total yield per plant with grafted cherry tomato '21A701' was 3,387g, which was 11%, 22% and 24% higher than the yield with 'B-blocking', non-grafted one and 'HSF4'. The stem diameter of '21A701' was thick with 8.26mm, whereas non-grafted one was thin with 7.23mm at 160 days after transplanting. The flowering position of '21LM' was 34% and 47% higher than the flowering position of 'B-blocking' and non-grafted one at 153 days after transplanting. The NO₃-N concentration in petiole sap of '21LM' was the highest with 1,746mg·L^-1 and non-grafted one and 'HSF4' were the lowest with 1,252mg·L^-1 and 1,245mg·L^-1 at 167 days after transplanting. The results indicated that rootstock/scion combinations in cherry tomatoes can affect the plant growth, yield, and the concentration of different NO₃-N in leaves at the late growth stage. Both '21A701' and '21LM' have vigorous root system, which influence the growth and yield increased.