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Molecular Phylogenetic Study of Anemone pendulisepala (Ranunculaceae)  

Lee, Chang Shook (Dept. of Life Science, Ewha Womens University)
Lee, Nam Sook (Dept. of Life Science, Ewha Womens University)
Yeau, Sung Hee (Dept. of Science Education, Ewha Womens University)
Publication Information
Korean Journal of Plant Taxonomy / v.36, no.4, 2006 , pp. 263-277 More about this Journal
Abstract
Anemone pendulisepala, recently described as a new species, is distributed in sympatry with A. reflexa, A. amurensis, and A. raddeana at Mt. taebeark and Mt. Baekdu. Anemone pendulisepala was previously proposed to be a hybrid species between A. reflexa and A. amurensis becaue it displavs overlapping features with them in involucre shape, petiole length, sepal apex and xylem shape, To verify the taxonomic status and to examine the hybridity of A. pendulisepala, sequences of ITS region of nuclear ribosomal DNA and the psba-trnH, rps16 and trnLF region of cpDNA from 36 accessions of 5 taxa including outgroup were analyzed. In maximum parsimony tree based on ITS sequences, A. pendulisepala had the same sequences of A. reflexa and was clustered with monophyletic A. amurensis, and then A. raddeana. Anemone pendulisepala was distinguished from the other taxa by having four base insertion in rps16 region, two species-specific bases and insertion in trnLF region. In the phylogenetic trees of combined cpDNA, A. pendulisepala showed monophyly with the bootstrap 100%. Anemone pendulisepala exhibited no polymorphism and shared no sequences with putative parental or related taxa examined in this study. Molecular data suggest that A. pendulisepala should be a distinct species, and no evidence of the hybridization was detectcd.
Keywords
Anemone pendulisepala; ITS; psbA-trnH; rps16; trnLF;
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