• Proceedings of the ESK Conference
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• 1997.10a
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• pp.142-151
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• 1997

본 연구는 주부들의 주활동공간인 부엌가구와 공간의 설계에 있어사용하기 편리하며 가사활동에 따 른 피로를 경감시키고 요통과 같은 주부직업병을 예방할 수 있는 부엌가구와 공간의 개발에 그 목적을 두었다. 연구는 먼저 수도권의 32평형 규모의 아파트150가구를 대상으로 설문조사를 실시하였으며, 만 24시간동안의 실제 가사활동을 Video로 촬영하여 동작분석을 실시하였다. 그 결과 주부들은 수납의 용 이성과 공간의 크기, 작업대의 높이 등을 가장 많은 개선사항으로 지적하였으며, 절반이상의 주부들이 앉은 자세에서 작업이 이루어졌으면 좋겠다고 응답하였다. 또한 동작분석 결과 작업의 70%이상이 개수 대를 중심으로 조리대와 가열대 주변의 한정된 공간에서정적으로 선 자세에서이루어지는 것으로 파악 되었다. 이러한 선 자세에서의 작업에 따른 피로도를 감소시키기 위하여 입좌식 의자를 도입하였으며, 개수대를 씽크대의 'ㄱ'자 코너에 위치하여 주방활동시의 이동거리를 단축하고자 하였다. 피로도의 경감 을 측정하기 위하여 입좌식 의자를 도입한 개선된 씽크대와 기존의 입식씽크대에서 가사활동시 요구되는 주부들의 에너지 대사량을 비교하기 위하여 심장박동수와 산소호흡량을 측정하였다. 실험결과에 따르면 주방에 입좌식의자를 도입한 경우가 가사활동에 요구되는 에너지 소모량이 심장박동수와 산소호흡량의 기준에서 각각 평균적으로 30%와 57.5% 이상이 감소하는 것으로 파악되어 이러한 부엌가구의 도입으로 주부들의 가사활동에따른 피로도가 절반 가까이 감소될 수 있음을 니타내었다. 또한 부엌가구의 재배치 에따른 가사활동시 이동거리가 평균적으로5.5%이상이 감소하는 것으로 나타났다. 본 연구결과를 주방설 계에 적용할 경우 가사활동의 효율성과 편리성이 향샹됨은 물론 가사활동에따른 피로도를 경감 시키고 요통 등의 주부직업병을 예방할 수 있을 것으로 기대된다.1일 1회씩 4회 투여한 군에서도 암수에 상관없이 전 농도 군에서 염색체이상을 나타내지 않아 유전독성을 나타내지 않음을 관찰하였다. 특히 vitamin C와 E의 병용투여는 상승적으로 적용하여 간세포손상을 더욱 억제시킴을 알 수 있었다.mance and on TFP(Total Factor Productivity) growth which is a pure measure of firm performance. To utilize the advantage of panel data, FEM(Fixed Effect Model) and REM(Random Effect Model) were used. The empirical result shows that the entropy index as a measurement of inter-business relatedness is not significant but technological relatedness index is significant. OLS estimates on pooled data were considerably different from FEM or REM estimates on panel data. By introducing interaction effect among the three variables for business portfolio properties, we obtained three findings. First, only VI (Vertical integration) has a significant positive correlation with ROS. Second, when using TFP growth as an dependent variable, both TR(Technological Relatedness) and f[ are signif

• Korean Journal of Microbiology
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• v.41 no.2
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• pp.140-145
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• 2005

Streptomyces natalensis ATCC27448 produces natamycin, a commercially important macrolide antifungal antibiotic. For molecular genetic study of S. natalensis, we have developed a system for introducing DNA into S. natalensis via conjugal transfer from Escherichia coli. An effective transformation procedure for S. natalensis was established based on transconjugation from E, coli ET12567/pUZ8002 using a ${\Phi}C31$-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 10 mM $MgCl_2$ using $6.25\times10^8$ of E.coli donor cells without heat treatment of spores. In addition, southern blot analysis of exconjugants and the sequence of plasmids containing DNA flanking the insertion sites from the chromosome revealed that S. natalensis contains a single ${\Phi}C31$ attB site and at least a secondary or pseudo attB site. Similar to the case of various Streptomyces species, a single ${\Phi}C31$ attB site of S. natalensis is present within an ORF encoding a pirin-homolog, but a pseudo-attB site is present within a distinct site (GenBank accession no. $YP\_117731$) and also its sequence deviates from the consensus sequences of attB sequence.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.1
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• pp.182-192
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• 2004

Gamma irradiation at 5 and 10 kGy was applied to Kwamegi (semi-dried Colobabis seira) for their possible hygiene quality and carried out genotoxicological safety. In vitro genotoxicological safety of each 5 and 10 kGy-irradiated Kwamegi was evaluated by Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) and E. coli WP2 uvrA reversion assay, SOS chromotest (Escherichia coli PQ37) and chromosome aberration test (Chinese hamster lung fibroblast cells) in the absence and presence of an exogenous metabolizing system (S9 mix). Gamma-irradiated samples were not different from nonirradiated-control to respective in vitro tests. And in vivo micronucleus test using ICR mice (male) micronucleus was not observed. Kwamegi exposed to 10 kGy-gamma ray revealed negative results in these three in vitro mutagenetic tests and in vivo micronucleus test up to 10,000 $\mu\textrm{g}$/plate, respectively. The results indicated that 5 and 10 kGy gamma-irradiated Kwamegi (semi-dried Colobabis seira) did not have mutagenicity.

• Biomedical Science Letters
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• v.5 no.1
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• pp.17-26
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• 1999

To evaluate the spreading possibilities of the marRAB mutation of E. coli Mar mutant among gram-negative bacilli, chromosomal marRAB mutations of Mar mutants were transduced by $\lambda$placMu9 into pUC19 (Lac$^{+}$, Ap$^{r}$) cloning site in another strains of E. coli or onto the chrmosome of S. typhimurium and P. aeruginosa, selected for transduction by Mar phenotype, Lac$^{-}$, or Ap$^{r}$, and tested for their antimicrobial resistance with or without addition of salicylate (SAL). Compared with wild type strains of JM109, NM522, harboring pUC19 or not, respectively, all strains of JM109 or NM522 carrying pUC19::marRAB mutation showed higher levels of antimicrobial resistance and SAL induction of Mar phenotype than those of wild type. However, in contrast to the original Mar mutants, there were some tendencies of decreased antimicrobial resistance of JM109 or NM522 harboring pUC19::marRAB mutation with SAL induction against chlorarnphenicol (Cm) and tetracycline (Tc), or Tc and ciprofloxacin (Cp), respectively. Almost the same results, as shown as the cases of E. coli JM109 or NM522, were obtained from all transductants of S. typhimurium and P. aeruginosa, except Cp, against which increased antimicrobial resistance with SAL induction was shown. This study, employed the methods of transformation or transduction among intercellular gene transfer methods between gram-negative bacteria, shows the evidences that suggest indirectly the spreading possibilities of marRAB mutation among gram-negative bacilli.

• Food Science and Preservation
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• v.13 no.4
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• pp.524-529
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• 2006

Gamma irradiation at 30 kGy was applied to cereal powders to evaluate their possible genotoxicity. The genotoxicity of 30 kGy-irradiated cereal powders was evaluated by Salmonella typhimurium reversion assay, chromosomal aberration test and in vivo micronucleus assay. The result were negative in the bacterial reversion assay with S. typhimurium TA98, IA100, TA1535 and TA1537. No mutagenicity was detected in the assay with and without metabolic activation. In chromosomal aberration tests with CHL cells and in vivo mouse micronucleus assay, no significant difference in the incidences of chromosomal aberration and micronuclei was observed between non-irradiated and 30 kGy-irradiated cereal powders. These result indicate that cereal powders irradiated at 30 kGy did not show any genotoxic effect under these experimental conditions.

• Journal of Life Science
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• v.24 no.10
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• pp.1046-1054
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• 2014

Beta-1,3-glucanase is widely used in various biotechnological and industrial processes, with over-production required to enable versatile utilization. We examined the overexpression of ${\beta}$-1,3-glucanase (EXGA) from Aspergillus oryzae using ${\delta}$-sequence-mediated integration. We constructed $pRS{\delta}$-exgA and $pRS{\delta}K$-exgA plasmids for integration of the EXGA gene into various chromosomes of Saccharomyces cerevisiae. These plasmids contain the ADH1 promoter for constitutive expression, a signal sequence (exoinulinase signal sequence [INU1 s.s]) for secretory production, and a ${\delta}$-sequence for integration of ${\beta}$-1,3-glucanase. The $pRS{\delta}$-exgA plasmid was transformed into the S. cerevisiae $BY4742{\Delta}exg1$ strain, and ${\beta}$-1.3-glucanase was stably overexpressed and secreted. Another plasmid, $pRS{\delta}K$-exgA, was introduced into the S. cerevisiae $BY4742{\Delta}exg1$ (YKY082) strain, and overexpression of ${\beta}$-1,3-glucanase was examined by inducible integration under geneticin selection. The activity of ${\beta}$-1,3-glucanase increased in accordance with a rise in the geneticin concentration, with 0.8 mg/ml of geneticin suitable for overexpression of ${\beta}$-1,3-glucanase. Subsequently, $pRS{\delta}K$-exgA was repeatedly transformed for sequential ${\delta}$-integration. The activity of ${\beta}$-1,3-glucanase reached about 0.063 unit/ml/$OD_{600}$, 0.095 unit/ml/$OD_{600}$, 0.131 unit/ml/$OD_{600}$ and 0.165 unit/ml/$OD_{600}$ by the first, second, third, and fourth round of integration, respectively. According to the increase in the activity of ${\beta}$-1,3-glucanase by sequential ${\delta}$-integration, the copy number (integration rate) of the EXGA gene also increased in various chromosomes. These results suggest that recombinant ${\beta}$-1,3-glucanase activity can be sequentially increased by repeated ${\delta}$-sequence integration.

• Radiation Oncology Journal
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• v.12 no.1
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• pp.1-8
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• 1994

When cells are exposed to low doses of a mutagenic or clastogenic agents. they often become less sensitive to the effects of a higher dose administered subsequently. Such adaptive responses were first described in Escherichia coli and mammalian cells to low doses of an alkylating agent. Since most of the studies have been carried out with human lymphocytes, it is urgently necessary to study this effect in different cellular systems. Its relation with inherent cellular radiosensitivity and underlying mechanism also remain to be answered. In this study, adaptive response by 1 cGy of gamma rays was investigated in three human lymphoblastoid cell lines which were derived from ataxia telangiectasia homozygote, ataxia telangiectasia heterozygote, and normal individual. Experiments were carried out by delivering 1 cGy followed by 50 cGy of gamma radiation and chromatid breaks were scored as an endpoint. The results indicate that prior exposure to 1 cGy of gamma rays reduces the number of chromatid breaks induced by subsequent higher dose (50 cGy), The expression of this adaptive response was similar among three cell lines despite of their different radiosensitivity. When 3-aminobenzamide, an inhibitor of poly (ADP-ribose) polymerase, was added after 50 cGy, adaptive responses were abolished in all the tested cell lines. Therefore it is suggested that the adaptive response can be observed in human lymphoblastoid cell lines, which was first documented through this study. The expression of adaptive response was similar among the cell lines regardless of their radiosensitivity. The elimination of the adaptive response by 3-aminobenzamide is consistent with the proposal that this adaptive response is the result of the induction of a certain chromosomal repair mechanism.

• Korean journal of food and cookery science
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• v.17 no.6
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• pp.617-624
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• 2001

Gamma irradiation (1-10 kGy) was applied to chicken for the evaluation of their microbiological safety and possible genotoxicity. In 3 kGy-irradiated sample, the growth of psychrophile was inhibited about 1.5 log cycles and no cells were recovered in total microbial counts. All kinds of contaminated microorganism were sterilized by 7 kGy-irradiation. Also, irradiation followed by freeze-storage at the same time was very effective in inhibiting bacterial growth. The genotoxicity of 10 kGy-irradiated chicken was evaluated by Salmonella Typhimurium reversion assay and in vivo micronucleus assay using mouse bone marrow cells. The results were negative in the bacterial reversion assay with S. Typhimurium TA98, TA100, TA1535, and TA1539. Clastogenic effects were not shown in vivo mouse micronucleus assay at 10 kGy-dose tested.

• The KIPS Transactions:PartA
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• v.13A no.1 s.98
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• pp.79-86
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• 2006

Recently the tagSNP selection problem has been researched for reducing the cost of association studies between human's diversities and SNPs. General approach for this problem is that all of SNPs are separated into appropriate blocks and then tagSNPs are chosen in each block. Marsel in this paper is the system that involved the concept of linkage disequilibrium for overcoming the problem that the existing block partitioning approaches have short of biological meanings. In most approaches, the contiguous regions, which recombinations have LD coefficient |D'| and then tagSNP selection step is performed. And MarSel guarantees the minimum tagSNP selection using entropy-based optimal selection algorithm when tagSNPs are chosen in each block, and enables chromosome-level association studies using efficient memory management technique when input is very large-scale dataset that is impossible to be processed in the existing systems.

• Journal of Sericultural and Entomological Science
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• v.17 no.1
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• pp.47-53
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• 1975

This experiment was carried out to obtain a marked larvae in sex-limited inheritance, using F$_1$ hybrid Bo ok X Chun san introduced from Japan. Sequence of backcrossing has been done through the earlier generation with a recessive character of plain marked larvae. 1. It is noted that genotypic segregation of sex-limited larval marking was observed in F$_4$ generation; female possesses larval marking and male shows a plain marking. 2. Larval marking heredity follows a diagram of segregation with an expected genotype such as ♀ ： (W$.$＋p)/Z$.$P/P and ♂ ： Z/Z$.$P/P. 3. It is observed that dissociation was occurred to produce female with a genotype of W/Z$.$P/P in segregation ana selection. 4. Abnormal ratio of sexuality is observed in the course of segregation and segregation and analysis of it is continuously under way. 5, It is observed that the difference of qualitative characteristics between female and male obtained from the original variety shows the same tendency as the normal marked variety.