• Title/Summary/Keyword: 염도 구배

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The distinct characteristics of phytoplankton growth response and their community structure following seven different nutrients addition in spring season of Jinhae Bay (춘계 진해만에서 농도 구배로 첨가한 영양염에 의한 식물플랑크톤 성장반응과 군집구조의 명확한 특징)

  • Son, Moonho;Baek, Seung Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.10
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    • pp.6567-6574
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    • 2015
  • In order to estimate the characteristics of the growth and composition of phytoplankton according to the available nutrients, we added nitrate (0, 1, 5, 10, 20, 50, $100{\mu}M$) and phosphate (0, 0.1, 0.5, 1, 2, 5, $10{\mu}M$) to field samples in a eutrophic site (St. 1) and an oligotrophic site (St. 22) in 2010 as well as a eutrophic site (St. 1, 5), a mesotrophic site (St. 19), and an oligotrophic site (St. 22) in 2011 at Jinhae Bay, Korea. The phytoplankton growth in the areas with additional nitrates and phosphates on St. 1 were significantly different from the control (One-way ANOVA:P<0.01). The dominant species at St. 1 in 2010 were Heterocapsa triquetra and Pseudo-nitzchia spp., to which nitrate and phosphate were added, respectively. The dominant species at St. 22 in 2010 differed between treatment conditions as follows: nitrate treatment Chaetoceros spp. (${\leq}10{\mu}M$), Thalassiosira spp. ($20{\mu}M$), and Pseudo-nitzchia spp.(${\geq}50{\mu}M$) for nitrate treatment; Cylindrotheca spp. ($2{\mu}M$) and Pseudo-nitzchia spp. ($5{\mu}M$) for phosphate treatment. Phytoplankton growth in 2011 according to the added nutrient were significantly different with treatment concentrations (One-way ANOVA: P<0.01). Moreover, the beginning of exponential growth in phytoplanktons was different between the eutro-mesotrophic sites (St. 1, 5, and 19) and the oligotrophic sites (St. 22) on day 2 and day 6 respectively. This implies that phytoplankton growth in the low nutrient condition may be retarded. The dominant species at St. 1 were Eucampia spp. and Chaetoceros spp. in the low nutrient treatment compared to Skeletonema spp., and Thalassiosira spp in the high nutrient treatment. The dominant species at St. 5 and St. 19 were mostly Skeletonema spp. and Chaetoceros spp. However, the dominant species at St. 22 was Thalassiosira spp.. The results of this study showed that phytoplankton growth and composition were different in areas with different nutrient characteristics resulting from the additional nutrients. Therefore, the nutrients additional algal assay could be indirectly explained why the biomass and composition of phytoplankton in Jinhae Bay has shown spatial differences.

Recovery of Residual LiCl-KCl Eutectic Salts in Radioactive Rare Earth Precipitates (방사성 희토류 침전물내 잔류하는 LiCl-KCl 공융염의 회수)

  • Eun, Hee-Chul;Yang, Hee-Chul;Kim, In-Tae;Lee, Han-Soo;Cho, Yung-Zun
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.8 no.4
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    • pp.303-309
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    • 2010
  • For the pyrochemical process of spent nuclear fuels, recovery of LiCl-KCl eutectic salts is needed to reduce radioactive waste volume and to recycle resource materials. This paper is about recovery of residual LiCl-KCl eutectic salts in radioactive rare earth precipitates (rare earth oxychlorides or oxides) by using a vacuum distillation process. In the vacuum distillation test apparatus, the salts in the rare earth precipitates were vaporized and were separated effectively. The separated salts were deposited in three positions of the vacuum distillation test apparatus or were collected in the filter and it is difficult to recover them. To resolve the problem, a vacuum distillation and condensation system, which is subjected to the force of a temperature gradient at a reduced pressure, was developed. In a preliminary test of the vacuum distillation/condensation recovery system, it was confirmed that it was possible to condense the vaporized salts only in the salt collector and to recover the condensed salts from the salt collector easily.

Isolation and purification of Cd-binding high molecular weight protein in rat liver

  • Kim, Bong-Hee;Chun, Ki-Jung
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1995.04a
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    • pp.126-126
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    • 1995
  • 본 연구는 중금속에 의한 생체내 독성기전을 연구하고자 흰쥐간의 상층액에서 카드뮴과 잘 결합하는 HMWP들을 분리, 정재하고 나아가 생화학적 특성을 밝히고 이단백질이 카드뮴에 의해 생성되는 것인지 아니면 간의 기존 단백질인지를 밝히고자 함을 연구의 목적으로 하였다. CdCl %2 (3mg/kg body wt.)을 3일간 ip injection시킨 후 흰쥐의 간을 적출하고 균질화하여 원심분리한 crude extract를 직접 Sephacryl S-100에 흡착시켜 10mM phosphate buffer(pH 7.0)으로 용출시켰다. 용출된 fraction tube를 UV spectrometer로 흡광도를 측정하고 atomic absorption spectrophotometer로 카드뮴량을 측정하여 카드뮴량이 높은 분획을 모아서 ion exchange column chromatography(DEAE-Sepharose)에 흡착시켜 염농도 구배로 chromatography를 실시하고, 음이온 교환수지에서 흡착되지 않고 용출된 분획을 ultrafiltration으로 농축시킨 후 S-Sepharose에 흡착시켜 염농도 구배로 chromatography를 실시한 결과 두 종류의 카드뮴 결합 단백질(Cd-BP)를 분리, 정제하였다.

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The distribution of sulfate and methane concentration and their vertical trend in the Ulleung Basin (동해 울릉분지의 황산염과 메탄의 농도 분포 및 심도에 따른 변화 양상)

  • Kim Ji-Hoon;Park Myong-Ho;Ryu Byong-Jae;Lee Young-Joo;Han Hyun-Chul;Cheong Tae-Jin;Oh Jae-Ho;Chang Ho-Wan
    • 한국신재생에너지학회:학술대회논문집
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    • 2005.06a
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    • pp.622-625
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    • 2005
  • 본 연구의 목적은 동해 울릉분지 천부퇴적층의 공극수와 메탄의 특징 및 상호작용을 규명하는데 있다. 울릉분지에서 채취한 코어에서 공극수를 추출하여 분석한 결과, 공극수의 황산염 농도가 퇴적물의 심도가 증가할수록 감소하며, 감소하는 경향은 크게 세 가지 (직선성, concave down, upward kink)로 나뉨을 알 수 있었다. 이는 모든 코어에서 황산염 환원작용이 일어나고 있음을 지시한다 황산염 농도의 수직적 구배를 이용하여 SMI (sulfate-methane interface) 심도를 계산하면, 남부울릉분지가 북부울릉분지보다 낮은 값을 갖는다. 반면에 메탄 농도는 퇴적물의 심도가 증가할수록 전반적으로 증가하며, 공간적으로는 남부 울릉분지가 북부울릉보지보다 높다. 또한 남부울릉분지에서 메탄가스 농도는 SMI 심도 아래에서 급격히 증가한다 메탄가스의 탄소 안정동위원소$(\delta^{13}C)$ 분석 값들은 대부분 $-60\%_{\circ}$이하로서 이는 메탄가스가 열기원 보다는 박테리아기원임을 지시해준다 또한 남부 울릉분지에서 메탄의 탄소 안정동위원소 분석 값들은 메탄농도가 증가할수록 낮은 값을 보여 주는 데 이러한 결과들은 남부 울릉분지에서 무산소 메탄 산화작용이 일어나고 있음을 지시하고, 메탄의 상향 분산 (diffusion)량이 북부 울릉분지보다 많이 일어난다는 것을 의미한다. 공극수내 황산염 이온 농도 구배와 메탄가스 농도를 종합적으로 고려할 때, 울릉분지에서 가스하이드레이트의 부존가능성은 북부 울룽분지보다 남부 울릉분지가 높은 것으로 추정된다.

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Geochemical characteristics of sediment, pore water, and headspace gas in the Ulleung Basin (울릉분지 퇴적물, 공극수 및 공기층 가스의 지화학적 특징)

  • Kim, Ji-Hoon;Park, Myong-Ho;Ryu, Byong-Jae;Lee, Young-Joo;Jin, Young-Keun
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.06a
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    • pp.373-376
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    • 2006
  • 본 연구의 목적은 동해 울릉분지의 제4기 후기 퇴적물 내의 유기물, 공극수와 메탄의 특징 및 상호작용을 규명하는데 있다. 연구지역에서 채취한 코어퇴적물을 원소 분석한 결과 C/N 및 C/S 비(wt. %)는 퇴적물 내 유기물이 주로 해양조류 기원을 가지고, 일반적인 해양 또는 정체 환경에서 퇴적되어Tdam을 지시한다. 그러나 Rock-Eval 열분석 결과는 유기물 기원이 육상식물(Type III)이고, 열적 성숙단계가 미성숙단계임을 보여준다. 이러한 원소분석과 열분석간의 상반된 결과는 유기물이 침강하는 동안 또는 퇴적 후 이루어진 강한 산화작용에 기인한 것으로 추정된다. 퇴적물 내 공극수의 황산염 농도가 퇴적물의 심도가 증가할수록 감소하며, 감소하는 경향은 크게 두 가지 (적선성, concave down)로 나누어진다. 이는 모든 코어에서 황산엽 환원작용이 일어나고 있음을 지시한다. 또한 직선선의 황산엽농도 구배는 무산소 메탄 산화작용(AMO)의 전형적인 특징이다. 황산염 농도의 수직적 구배를 이용하여 SMI(sulfate-methane interface) 심도를 계산하면, 남부울릉분지의 코어 (03GHP-01, 03GHP-02; <3.5mbsf)가 북부울릉분지 코어(01GHP-05, 01GHP-07, 03GHP-03, 03GHP-04, 03GHP-05; > 6mbsf)보다 낮은 값을 갖는다. 위와 같은 SMI 심도차는 메탄의 상부 분산량과 밀접한 관련있는 것으로 추정된다. 메탄가스의 탄소 안정동위원소 $({\delta}^{13}C)$ 분석값들은 -83.5%o에서 -69.5%o의 범위를 가지고 있고, 이산화탄소 환원작용($CO)_2$ reduction)에 의한 생물 (biogenic) 기원임을 지시한다.

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Assessment of Phytoplankton Viability Along the Salinity Gradient in Seomjin River Estuary, Korea (섬진강 하구역에서 염분구배에 따른 식물플랑크톤 활성도 평가)

  • Lim, Youngkyun;Baek, Seung Ho
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.5
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    • pp.513-523
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    • 2017
  • We evaluated the viability of phytoplankton along the salinity gradient in the flood and ebb tides of spring tide of February and the ebb tide of neap tide of March 2017 in the Seomjin River Estuary. Additional laboratory experiments were also conducted to determine the reason of the pH changes along the salinity gradient using the field natural sample in February. In field, saltwater was well mixed at downstream vertically and the salinity gradient was horizontally appeared toward upstream of freshwater zone. There were strong negative correlations between salinity and nutrient (nitrate + nitrite R=0.99, p<0.001, and silicate R=0.98, p<0.001), implying that those two nutrients of freshwater origin were gradually diluted with mixing the saltwater. On the other hands, relatively high phosphate concentration was kept in the stations of saltwater over 15 psu, indicating that it was caused by resuspended sediments of Gwangyang Bay and downstream by tidal water mixing.Among phytoplankton community structure in winter, Eucampia zodiacus have occupied to be c.a. 70 % in the most stations. Based on the field survey results for survivability of phytoplankton by phytoPAM instrument, there was positive correlations between salinity and chlorophyll a (R=0.82, p<0.001) and, salinity and active chlorophyll a (R=0.80, p<0.001), implying that the dominant marine diatom species may have significantly damaged in low salinity conditions of upstream. Also, maximum mortality rate of phytoplankton caused by low salinity shock was appered to be 75% in the upstream station. In particular, the pH in spring tides of February had tended to increase with high phytoplankton accmulated stations, suggesting that it was related with absorption of $CO_2$ by the photosynthesis of dominant diatom. In laboratory experiments, phytoplankton mass-mortality caused by low salinity shock was also occurred, which is confirmed with reducing the photosynthetic electron transport activity. Following the phytoplankton mass-mortality, bacteria abundance was significantly increased in 24 hours. As a result, the mass-proliferating bacteria can produce the $CO_2$ in the process of biodegradation of diatoms, which can lead to pH decrease. Therefore, marine phytoplankton species was greatly damaged in freshwater mixing area, depending on along the salinity gradient that was considered to be an important role in elevating and reducing of pH in Seomjin River Estuary.

향류식 역삼투 농축공정을 이용한 NaCl 용액의 농축연구

  • 조한욱
    • Proceedings of the Membrane Society of Korea Conference
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    • 1997.04b
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    • pp.3-10
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    • 1997
  • 제염농축공정은 증류, 증발법을 일반적으로 사용하나, 에너지 절약차원에서 전기투석 (Electro dialysis)장치를 증발기 선단에 도입한 복합공정을 국내에서도 사용하고 있는 실정이다. 그러나, 전기투석장치는 전기적인 소모와 막의 재생, 교체처리비가 문제점이 되므로 역삼투 장치를 제염농축 공정 최선단에 도입한 복합공정을 이용할 경우 전기 투석 및 증발 복합공정에 비해 40%의 에너지 절감 효과를 기대할 수 있다. 이와 같은 장점에도 불구하고 역삼투 공정은 공급용액의 삼투압보다 큰 적용압력을 막표면에 가하여 물질분리를 수행하므로 농축공정에서 유발되는 배제액 농도의 상승은 삼투압의 증가를 일으켜 실적적용압력의 효과를 떨어뜨리게 되며 결과적으로 농축효과를 감소시키게 된다. 본 연구에서는 효과적인 염농축 공정을 위하여 막모듈 투과부에 고농도 삼투압 감소액(osmotic sink solution)을 향류식(막투과흐름을 맞받아치며 흐르는 방식)으로 유입시키는 향류식 역삼투 (counter-current reverse osmosis, CCRO) 나권형 모듈을 고안 제작하였으며, 제작된 모듈을 기존 역삼투 공정과 향류식 염삼투 공정에 적용하여 염농축도의 성능을 상호 비교하고 염농축에 관계되는 공급농도, 공급유량, 투과유량, 배제유량, 향류 유입유량, 압력구배, 삼투압차 및 농축단수 등의 인자들을 이용하여 두 공정에 대한 염농축 분리조작의 제반조건과 제작된 모듈 내의 농축관련 특성을 실험 및 수치적으로 비교, 고찰하였다.

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Soil Salinity and Continuum Distribution of Vegetation on the Three Reclaimed Tidal Flats of Kyonggi-Bay in the Mid-West Coast of Korea (한국 중부 서해안 경기만 일대 3개 간척지의 토양 염농도와 식생의 연속분포)

  • Kim, Eun-Kyu;Chun, SoUl;Joo, Young-K.;Jung, Yeong-Sang;Jung, Hyeung-Gun
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.2
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    • pp.83-93
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    • 2008
  • Assessing for flora distribution is necessary for land management and environmental research in reclaimed lands. This study was conducted to find out the relationship between vegetation distribution and soil salinity on three reclaimed tidal flats of Kyonggi-bay in the mid-west coast of Korea. We investigated the soil salinity and identified the vegetation at the continuum distribution spots, and describe the characteristics of continuum distribution. On the reclaimed tidal flats, spatial variation of vegetation formed partially, however as the result for connection of each spatial variation along with the soil salinity, continuum distribution formed and it was overlapped edaphic gradient with vegetation distribution, it means that the continuum distribution correspond with soil salinity gradient, as the evidence high salt tolerance species occurred at high saline spots, non salt tolerance species occurred at low saline spots. On the aged reclaimed tidal flats, continuum type was various and also clearly distinguished but it was not clear on the early stage of reclamation. The continuum distribution distinguished sequential and non-sequential type. Sequential type started from high saline zone and connected to low saline zone gradually, on this type, vegetation changed from pioneer halophyte to facultative halophyte and glycophyte along with the salinity gradient. Non-sequential type formed by non-sequential change of soil salinity, on this type, vegetation distribution was non-regular form because it has not changed gradually. In the aged reclaimed land, vegetation wilted zone existed with high salinity, and continuum distribution started from this zone with bare patch.

Aquifer Characterization Using Seismic Data on the Aquistore CCS Project, Canada (캐나다 아퀴스토어 탄성파자료를 통한 이산화탄소 지중저장 연구지역 대수층 특성화)

  • Cheong, Snons;Kim, Byoung-Yeop;Shinn, Young Jae;Lee, Ho-Yong;Park, Myung-Ho
    • Economic and Environmental Geology
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    • v.47 no.6
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    • pp.625-633
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    • 2014
  • The Aquistore project is the world's first commercial capture, transportation, utilization and storage project of post-combustion $CO_2$ from a coal-fired thermo electric power plant, and the proposed storage is a saline aquifer at a depth of about 3,500 m. Deep saline aquifer, compared to hydrocarbon reservoir, provides the great volumetric potential for storage of $CO_2$ anywhere in the world, therefore the research results from the project may be exported globally to other sites. Geological $CO_2$ storage characterization for saline aquifer instead of hydrocarbon reservoir needs to estimate the geophysical properties of subsurface geology. This study calculated the geophysical property of water-saturated formation by applying amplitude variation analysis developed from oil and gas exploration. We correlated horizon tops at the well logs to seismic traveltime of 1,815 and 1,857 ms as Winnipeg and Deadwood formations. Gradient analysis from seismic traces showed correlation coefficient of 45 - 81 % on amplitude variation with respect to incident angle. Crossplot of intercept and gradient shows the inverse proportional trend which represents typical water saturated sediments. Product attribute of intercept and gradient described the base of wet sediment. Poisson's ratio change attribute increased at the top of target area satisfying with wet sediment and decreased at the top of basement in a dry rock bed.