• Title/Summary/Keyword: 연쇄

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The detection of Human Papillomavirus (HPV) by the polymerase chain reaction(PCR) in head and neck cancers (두경부암에서 중합효소 연쇄반응을 이용한 유두종 바이러스의 검출)

  • ;;;Richard E Hayden;David B Weiner
    • Proceedings of the KOR-BRONCHOESO Conference
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    • 1993.05a
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    • pp.87-87
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    • 1993
  • Polymerase chain reaction is widely used as a powerful tool in modern molecular biology. As there is agreement that the HPV is an important factor in the head and neck cancers, the detection of HPV DNA sequence in the head and neck cancer tissue has been tried in several ways. We used the PCR to detect the E1 open reading frames of the HPV in paraffin-embedded tissue of the patients with the head neck cancers. Eleven of the fifty-four tested samples (30%) showed positive result. We have analysed the clinical courses and characteristics related with Human Papillomavirus in those patients.

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Analytical Study on Effect of Floor Slab for Progressive Collapse Resistant Capacity of Steel Moment Frames (철골모멘트골조의 연쇄붕괴저항성능에 대한 바닥슬래브의 효과에 관한 해석적 연구)

  • Kim, Seonwoong
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.27 no.1
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    • pp.27-35
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    • 2014
  • In this study, an improved energy-based nonlinear static analysis method are proposed to be used for more accurate evaluation of progressive collapse potential of steel moment frames by reflecting the contribution of a double-span floor slab. To this end, the behavior of the double-span floor slab was first investigated by performing material and geometric nonlinear finite element analysis. A simplified energy-absorbed analytical model by idealizing the deformed shape of the double-span floor slab was developed. It is shown that the proposed model can easily be utilized for modeling the axial tensile force and strain energy response of the double-span floor slab under the column-removal scenario.

Efficient Transmission Schemes in UWB Based Ship Area Networks (UWB기반 선박 네트워크에서 효율적인 전송 기법)

  • Kim, Jin-Woo;Kwon, Jang-Woo;Jeong, Min-A;Lee, Seong Ro
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.39C no.11
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    • pp.1151-1158
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    • 2014
  • In this paper, a concatenated frame algorighm for improving transmission efficiency in WiMedia protocol is studied. And an efficient transmission scheme to reduce the degradation of network performance by the loss of mini-frame is proposed in this paper. The proposed scheme can reduce total energy consumption and improve the transmission efficiency since it only retransmits mini-frame in which error is occurred. The simulation results show that proposed protocol can enhance the throughput performance and improve energy efficiency by minimizing the retransmission of mini-frame.

Strategy for Improvement of Disaster Response System of Hybrid Disaster in Korea (국내 대형복합재난의 재난대응체계 개선방향)

  • Song, Chang-Young;Park, Sang-Hoon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.21 no.3
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    • pp.45-53
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    • 2017
  • Hybrid disaster refers to a type of unexpectedly large-scale disaster that results from continuous or simultaneous occurrence of natural and social disasters. Since hybrid disaster is a completely new type of disaster that has simultaneity and continuity, it requires a different management method compared to the conventional disaster management method. Accordingly, this study suggested policy directions for hybrid disaster response system of Korea after reviewing hybrid disaster response systems in developed foreign nations.

Analyzing the Industrial Contribution of Human Resource Development Programs in Renewable Energy Sector using Supply-side Input-Output Model (인력공급지장의 측면으로 본 신재생에너지 인력양성의 산업기여도 분석)

  • Lee, You-Ah;Kim, Jin-Soo;Heo, Eun-Nyeong
    • New & Renewable Energy
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    • v.5 no.4
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    • pp.68-73
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    • 2009
  • 국가 에너지 안보 확보와 친환경적이고 지속적인 '저탄소 녹색성장'의 기반을 마련한다는 측면에서 신재생에너지 분야의 인력양성은 시급하고도 중요한 당면 과제이다. 본 연구에서는 신재생에너지인력양성이 원활하게 공급되지 않았을 경우 발생할 수 있는 인력공급지장효과를 분석하기 위하여 산업연관분석의 공급유도모형을 유도하고 2006년 신재생에너지 인력양성사업 배출인원을 기준으로 실증분석을 실시하였다. 또한 공급유도형의 감응도 계수와 영향력계수 분석을 통해 신재생에너지 관련 사업과 타 산업 간의 전후방연쇄효과를 비교분석하였다. 연구 수행 결과 신재생에너지 관련 산업의 평균 영향력계수는 1.37, 평균 감응도 계수는 0.96으로 최종 수요적 제조업의 성격을 띠고 있는 것으로 나타났다. 또한, 2006년 신재생에너지 인력양성사업에 의해 배출된 336명의 인력이 공급되지 않았을 경우 공급지장비용은 총 230억이며, 8개 신재생에너지원 중 태양열과 연료전지에 인력 공급지장비용이 타 에너지원에 비해 상대적으로 높은 것으로 분석되었다. 산업분류 기준으로는 일반목적용 기계 산업과 전기기계 장치 산업이 신재생 에너지 인력공급 장애에 따른 지장비용이 높은 것으로 분석되었다.

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Development of Electrical Construction Cost Index Applied Chain-Weighted Method (연쇄방식 전기공사비지수 개발에 관한 연구)

  • Park, Houng-Hee;Choi, Seung-Dong;Hyun, So-Young;Park, Min-Young
    • Korean Journal of Construction Engineering and Management
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    • v.15 no.5
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    • pp.49-60
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    • 2014
  • Electrical construction cost index has been applied fixed-weighted method. But fixed-weighted method can't faithfully reflect industrial structure changes. Because the weighted value and price index of fixed-weighted method is fixed on the basic period. Electrical construction cost index is composed of the cost of labor and material. So it fluctuates sharply whenever the construction association of korea announces the laborer's wage of electrical construction. And it depends on only the producer price index changes that is related to electrical construction since then. So a study is focused on developing electrical construction cost index applied chain-weighted method. Because chain-weighted method can reflect the realities of the electrical construction and alleviate the sudden changes of labor cost with link index. We verify that chain-weighted method relieves the step states of electrical construction cost index applied fixed-weighted method.

PCR-Based Sensitive Detection and Identification of Xanthomonas oryzae pv. oryzae (중합효소연쇄 반응에 의한 벼 흰잎마름병균의 특이적 검출)

  • Lee, Byoung-Moo;Park, Young-Jin;Park, Dong-Suk;Kim, Jeong-Gu;Kang, Hee-Wan;Noh, Tae-Hwan;Lee, Gil-Bok;Ahn, Joung-Kuk
    • Microbiology and Biotechnology Letters
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    • v.32 no.3
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    • pp.256-264
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    • 2004
  • A new primer set was developed for the detection and identification of Xanthomonas oryzae pv. oryzae, the bacterial leaf blight (BLB) pathogen in rice plant. The nucleotide sequence of hpaA gene was determined from X. o. pv. oryzae str. KACC10331, and the sequence information was used to design primers for the application of the polymerase chain reaction (PCR). The nucleotide sequence of hpaA from X. o. pv. oryzae str. KACC 10331 was aligned with those of X. campestris pv. vesicatoria, X. campestris pv. campestris, X. axonopodis pv. citri, and X. axonopodis pv. glycines. Based on these results, a primer set(XOF and XOR) was designed for the specific detection of hpaA in X. o. pv. oryzae. The length of PCR products amplified using the primer set was 534-bp. The PCR product was detected from only X. o. pv. oryzae among other Xanthomonas strains and reference bacteria. This product was used to confirm the conservation of hpaA among Xanthomonas strains by Southern-blotting. Furthermore, PCR amplification with XOF and XOR was used to detect the pathogen in an artificially infected leaf. The sensitivity of PCR detection in the pure culture suspension was also determined. This PCR-based detection methods will be a useful method for the detection and identification of X. o. pv. oryzae as well as disease forecasting.

Transferable R plasmid of Streptococci Ioslation from Diseased Olive Flounder (Paralichthys olivaceus) in Jeju (제주도 양식넙치병어에서 분리된 연쇄상구균의 약제내성 전이성 plasmid)

  • Kim, Jong-Hun;Lee, Chang-Hoon;Kim, Eun-Heui
    • Journal of fish pathology
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    • v.19 no.3
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    • pp.267-276
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    • 2006
  • Seventy-five streptococci were isolated from diseased olive flounder, Paralichthys olivaceus in Jeju. Their drug susceptibility and transferable multiple drug resistance were characterized. All isolates were resistant to flumequine (AR) and oxolinic acid (OA) and 26 isolates (34.7%) showed 4~6 multiple resistance of ampicillin (ABPC), AR, doxycycline (DOXY), erythromycin (EM), norfloxacin(NOR), OA and oxytetracycline (OTC) in various combinations. pST9 of a transferable R plasmid was detected from a multiple drug resistance strain, Streptococcus sp., ST9 originated from diseased flounder in Jeju, previously. We performed DNA hybridization to know the distribution of plasmid with the same DNA structure as pST9 in streptococci. Thirteen out of 60 isolates analyzed were positive in colony DNA hybridization and the part of bacteria isolated from raw meal was also hybridized with pST9. It suggested that raw meal is one of the origin of the resistance plasmid and R plasmid with DNA structure differing from pST9 is also involving in multiple drug resistance of the streptococci. In conjugation experiment, we found transferable R plasmid carrying OTC, DOXY and/or EM resistance determinant in the 13 resistance strains. all of the streptococci carrying the transferable R plasmid were similar in RAPD patterns. However, pST -type R plasmid was rare in S. iniae most frequently appearing in flounder farm.

Characteristics of $\beta$-Streptococcus spp. Isolated in Cultured Flounder (Paralichthys olivaceus) of Jeju Island (제주산 양식넙치 (Paralichthys olivaceus)로부터 분리된 $\beta$-용혈성 연쇄구균 ($\beta$-Streptococcsus spp.)의 특성)

  • HEO Moon-Soo;SONG Choon Bok;LEE Jehee;YEO In-Kyu;JEON You-Jin;LEE Jung-Jae;CHUNG Sang-Chul;LEE Ki-Wan;RHO Sum;CHOI Kwang-Sik;LEE Young Don
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.4
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    • pp.365-369
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    • 2001
  • Streptococcus spp. of bacterial pathogen of fish were isolated from the cultured flounder (Paralichthys olivaceus) in fish farm of Jeju Island. Clinical signs of the infected flounder which are the most commons symptoms are as follows: erratic swimming, darkening of the body colour, unilateral or bilateral exophthalmia, corneal opacity, hemorrhages in the opercular and the bases of the fins, and the ulceration of the body surface. Biochemical characteristics of pathogenic fish Streptococcus spp, were gram positive, spherical form, catalase negative, oxidase negative and $\beta$-haemolytic, respectively, The viable cells counted from the tissue of the diseased flounder were the largest in the order of the ulcer, the kidney, the blood and the brain, The drugs used were ampicillin, ciprofloxacin, doxycycline, gentamycin, tetracycline, erythromycin, streptomycin and oxytetracycline, Streptococcus spp. were found to be sensitive to ampicillin, ciprofloxacin, doxycycline and gentamycin, but were resistant to tetracycline, erythromycin, streptomycin and oxytetracycline. The pathogenicity of Streptococcus spp. on the cultured flounder with an abdominal cavity injection was high. The haemolytic activity of the toxin against the sheep red blood cells reached the maximum after 30 min incubation at $37^{\circ}C$ or $50^{\circ}C$. The toxin showed highest activity at pH $5.5\sim6.5$.

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RELATIONSHIP BETWEEN SALIVARY CARIES-RELATED TESTS AND DENIAL CARIES EXPERIENCE IN KOREAN DENTAL COLLEGE STUDENTS (타액 우식 관련 검사와 치아 우식 경험과의 관계에 관한 연구)

  • Kim, Jae-Gon;Kim, Young-Shin;Baik, Byeong-Ju;Yang, Yeon-Mi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.32 no.1
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    • pp.67-74
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    • 2005
  • The aim of this study was to analyze the relationship between salivary flow, salivary buffer capacity, level of mutans streptococci and dental caries experience in Korean dental college students. A total of 81 students of the school of dentistry, Chonbuk national university, mean age of 26.1 years, were subjected to oral examination to establish the DMFT and DMFS according to WHO guidelines. Saliva samples from the students were collected for quantitating stimulated and unstimulated salivary flow rate. Buffering capacity was estimated using a commercial colorimetric strip test, and number of mutans streptococci was determined from stimulated saliva using a strip mutans test. The means of DMFT and DMFS were 6.57 and 12.65, respectively. The stimulated salivary flow rate was correlated with DMFT(${\acute{o}}\;=\;-0.219$) and high levels of salivary mutans streptococci were significantly correlated with higher DMFT and DMFS scores(P<0.05). Level of mutans streptococci was significantly correlated with dental caries experience, in both DMFT and DMFS score, and buffering capacity was inversely correlated to DMFT score. However, unstimulated salivary flow rate was not correlated with caries experience.

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