• Environmental Analysis Health and Toxicology
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• v.19 no.3
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• pp.235-240
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• 2004

본 연구는 비만세포의 일종인 RBL-2H3 세포주에 대한 hexamethylene bisacetamide (HMBA)의 세포독성 유도 기전이 telomerase의 활성과 관계가 있다는 것에 대한 보고이다. RBL-2H3 세포주에 5mM HMBA를 처리시 그 세포주의 세포성장이 억제 되었다. 기전연구로서 HMBA는 telomerase활성을 억제 하였으며 RT-PCR에 의한 결과 telomerase mRNA발현의 억제에 의한 것이었다. Madl은 telomerase의 발현을 억제하는 억제인자로 잘 알려져 왔으며 이를 확인해본 결과 Madl은 발현은 유의성있게 증가 되었다. 이를 종합해보면, HMBA에 의한 RBL-2H3 세포주에 대한 세포독성은 Madl 발현의 증가에 따른 telomerase발현의 억제에 기인하는 것으로 밝혀졌다.

• KSBB Journal
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• v.18 no.6
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• pp.485-489
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• 2003

Hepatocyte growth factor (HGF) is a mesenchymal-derived cytokine. It exerts a motogenic effect on various target cells, which is displayed either by cell scattering, locomotion, and migration during the wound repair process of cultured cells, or invasiveness through the extracellular matrix, in vitro. Although it is known that HGF influences the motogenic effect of endothelial cells, the precise effects of HGF during migration are still poorly understood. To elucidate the role of HGF in endothelial cell migration, the effect of HGF on endothelial cell migration and MMPs and plasmin production were studied. We found that HGF induces the migration of cultured endothelial cells through increased MMPs and plasmin secretion.

• Korean Journal of Food Science and Technology
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• v.48 no.1
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• pp.66-71
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• 2016

Delphinidin is a blue-red pigment and one of the major anthocyanins in plants. It plays an important role in anti-oxidant, anti-inflammatory, anti-mutagenic and anti-cancer properties. In this study, we investigated the inhibitory effects of delphinidin on vascular endothelial growth factor (VEGF) gene expression, an important factor involved in angiogenesis and tumor progression in human prostate cancer. Delphinidin decreased levels of epidermal growth factor (EGF)-induced VEGF mRNA expression in PC-3M cells. The expression of the EGF-induced hypoxia inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$) and signaling transducer and activator of transcription 3 (STAT3) proteins, which are the major transcription factors for VEGF, were inhibited by delphinidin. In addition, delphinidin decreases HRE-promoter reporter gene activity, suggesting that delphinidin can suppress the transcription of HIF-$1{\alpha}$ under EGF induction, leading to a decrease in the expression of VEGF. Delphinidin specifically suppressed the phosphorylation of Akt, p70S6K, and 4EBP1, but not the phosphorylation of EGFR. Therefore, our results suggest that delphinidin may inhibit human prostate cancer progression and angiogenesis by inhibiting HIF-$1{\alpha}$, STAT3 and VEGF gene expression.

• 대한위암학회:학술대회논문집
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• 2003.04a
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• pp.22-22
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• 2003

• Bulletin of Food Technology
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• v.18 no.1
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• pp.110-119
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• 2005

• Korean Journal of Poultry Science
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• v.1 no.1
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• pp.5-11
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• 1973

1. 닭 우모색에 있어서 흑색 melanin이 착색을 이압하는 I gene은 8주시 체중을 억제하는 작용을 하였다. 2. Barring을 나타내는 B gene이 I gene과 E gene이 나타났을 때 8주시 체중을 더욱 억제하였다. 3. 확장 흑색인자인 E gene과 I gene이 상호작용을 할 때에 8주시 체중을 억제하나 I gene이 억제작용 하는 이상의 작용은 하지 않았다. 4. I gene의 성장률 억제작용은 8주시 체중이 900-1,100g였을 때 평균 암탉에 있어서 37g 수탉에 있어서 25g 수탉에 있어서는 40g이었다. 이 시기의 성장률에 대하여 I와 E B S.의 공동작용은 더욱 억제작용을 하였는데 890-1,070g시에 암탉에 있어서는 45g, 수탉에 있어서는 53g였다. (중략)

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.615-624
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• 2022

In this study, we assessed anti-oxidant activity, anti-microbial, inhibition of biofilm formation and inflammatory factors(nitric oxide, interleukin-6, interleukin-8) inhibitory effect of Perillae semen hydrothermal extract(PSW) and three kinds of Perillae semen supercritical fluid extract(PSSs) extracted by controlling temperature with no variation of pressure. Compared with PSW, PSSs had significantly lower minimal inhibitory concentrations(MICs) against Staphylococcus aureus(S. aureus) and the ability of PSSs to inhibit formation of biofilm was also superior. PSSs reduce the production of inflammatory mediator and inflammatory cytokines significantly compared to PSW. We suggest, therefore, Perillae semen supercritical fluid 45℃ extract which showed the best anti-microbial, inhibition of biofilm formation, and inhibition of inflammatory factors production among the supercritical fluid extracts could be used for protecting patients with atopic dermatitis from pruritus and transepidermal water loss as a functional ingredient from nature.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.6
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• pp.681-687
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• 2017

This study was focused on the anti-inflammatory activities of Annona muricata leaf ethanol extracts (AME). Inflammation of macrophages was induced by lipopolysaccharide (LPS) treatment, and various inflammation-mediated factors [cytokines and nitric oxide (NO)] were measured. AME treatment significantly reduced LPS-induced NO, cytokine levels [interleukin (IL)-6, tumor necrosis $factor-{\alpha}$ and $IL-1{\beta}$], and expression of inducible NO synthase and cyclooxygenase-2 in a dose-dependent manner. Mechanical studies showed that AME treatment inhibited activation of mitogen-activated protein kinase and nuclear factor $(NF)-{\kappa}B$ in macrophages treated with LPS. From these results, AME treatment strongly inhibits LPS-induced inflammation through inhibition of $NF-{\kappa}B$ activation, suggesting AME could be a potential candidate for treatment of inflammatory disease as a nutraceutical drug.

• Journal of Life Science
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• v.14 no.1
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• pp.154-162
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• 2004

Thrombospondin-1 (TSP-1), a negative regulator in tumor growth and angiogenesis, is cell-type specifically regulated and at transcriptional level by external stimuli. Previously, we found that phorbol 12-myristate 13-acetate (PMA) suppressed TSP-1 expression in porcine aortic endothelial (PAE) cell, but enhanced in hepatoma cell line, Hep 3B cell. A region between -767 and -723 on the tsp-1 promoter was defined as a responsive site to the suppression in PAE cell. eased on the previous results, the molecular mechanism of TSP-1 expression was determined by characterizing interactions between cis-elements and trans-factors using three overlapped oligonucleotide probes, oligo a-1 (from -767 to -738), a-2 (-759 to -730) and a-3 (-752 to -723). The results from electromobility shift assay showed that PMA-induced suppression of TSP-1 transcription in PAE cell might be caused via a negative regulator binding to the region from -752 to -730 and additionally generated by lacking two positive regulators binding to the sites from -767 to -760 and from -752 to -730. Especially, PMA enhanced the binding ability of the negative regulator to the site from -752 to -730 in PAE cell, but anti-c-Jun did not affected its binding ability.

• Journal of Food Hygiene and Safety
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• v.31 no.4
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• pp.286-293
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• 2016

This study is an experiment for gastric protective effects of ursolic acid. In order to identify the effects of ursolic acid on gastrointestinal disorder, acute and chronic gastritis were also observed using HCl ethanol and indomethacin-induced gastric lesion models, respectively. As for gastric acid, it was also identified through proton pump ($H^+/K^+-ATPase$) inhibiting activity. In regards to protective factor for gastric damage, prostaglandin $E_2$ ($PGE_2$) was quantitatively analyzed. Antibacterial activity experiment was done on Helicobacter pylori (H.pylori), which is known to be the causing factor of chronic gastritis, gastric ulcer and gastric cancer. By making use of AGS cell, it was confirmed that ursolic acid was involved in apoptosis of gastric cancer cell through 4',6-diamidino-2-phenylindol (DAPI) staining and flow cytometry analysis. As a result, ursolic acid reduced gastric lesions caused by HCl ethanol and indomethacin. Ursolic acid inhibited acid secretion by inhibiting proton pump ($H^+/K^+-ATPase$), which is the gastric acid secreting enzyme involved at the final phase of gastric acid secretion. And ursolic acid was identified with gastric mucosa protection effects by increasing the concentration of $PGE_2$, a protective factor of gastric mucosa preservation. The antibacterial activity on H. pylori, which is aggressive factor in gastrointestinal disorder, ursolic acid showed inhibitory effects on H. pylori colonization. In the DAPI nuclear staining, unlike the control group, shape of the nucleus has deformed, and has been observed either shrinked cell or chromatin condensation phenomenon. In the Flow cytometry assay, confirmed the growth rate of apoptosis in a concentration-dependent manner.