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http://dx.doi.org/10.5352/JLS.2004.14.1.154

Suppression of Thrombospondin-1 Expression by PMA in the Porcine Aortic Endothelial Cells  

Chang, Seo-Yoon (Department of Biochemistry, College of medicine, The Catholic University of Korea)
Kang, Jung-Hoon (Department of Biochemistry, College of medicine, The Catholic University of Korea)
Hong, Kyong-Ja (Department of Biochemistry, College of medicine, The Catholic University of Korea)
Publication Information
Journal of Life Science / v.14, no.1, 2004 , pp. 154-162 More about this Journal
Abstract
Thrombospondin-1 (TSP-1), a negative regulator in tumor growth and angiogenesis, is cell-type specifically regulated and at transcriptional level by external stimuli. Previously, we found that phorbol 12-myristate 13-acetate (PMA) suppressed TSP-1 expression in porcine aortic endothelial (PAE) cell, but enhanced in hepatoma cell line, Hep 3B cell. A region between -767 and -723 on the tsp-1 promoter was defined as a responsive site to the suppression in PAE cell. eased on the previous results, the molecular mechanism of TSP-1 expression was determined by characterizing interactions between cis-elements and trans-factors using three overlapped oligonucleotide probes, oligo a-1 (from -767 to -738), a-2 (-759 to -730) and a-3 (-752 to -723). The results from electromobility shift assay showed that PMA-induced suppression of TSP-1 transcription in PAE cell might be caused via a negative regulator binding to the region from -752 to -730 and additionally generated by lacking two positive regulators binding to the sites from -767 to -760 and from -752 to -730. Especially, PMA enhanced the binding ability of the negative regulator to the site from -752 to -730 in PAE cell, but anti-c-Jun did not affected its binding ability.
Keywords
Thrombospondin-1; c-Jun; gene expression; promoter activity; cis-elements; trans-acting elements;
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