The Journal of the Convergence on Culture Technology (문화기술의 융합)

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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The Effect of Anti-microbial and the Inhibitory Effect of Biofilm Formation and Inflammatory Factors Production of Perillae semen Supercritical Fluid Extracts

초임계 자소자추출물의 항균효과와 바이오필름, 염증매개인자 생성 억제 효능

  • 이광원 (을지대학교 시니어헬스케어학과) ;
  • 박신성 (을지대학교 미용화장품과학과) ;
  • 박수인 (을지대학교 미용화장품과학과) ;
  • 신문삼 (을지대학교 미용화장품과학과)
  • Received : 2022.08.14
  • Accepted : 2022.10.10
  • Published : 2022.11.30
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Abstract

In this study, we assessed anti-oxidant activity, anti-microbial, inhibition of biofilm formation and inflammatory factors(nitric oxide, interleukin-6, interleukin-8) inhibitory effect of Perillae semen hydrothermal extract(PSW) and three kinds of Perillae semen supercritical fluid extract(PSSs) extracted by controlling temperature with no variation of pressure. Compared with PSW, PSSs had significantly lower minimal inhibitory concentrations(MICs) against Staphylococcus aureus(S. aureus) and the ability of PSSs to inhibit formation of biofilm was also superior. PSSs reduce the production of inflammatory mediator and inflammatory cytokines significantly compared to PSW. We suggest, therefore, Perillae semen supercritical fluid 45℃ extract which showed the best anti-microbial, inhibition of biofilm formation, and inhibition of inflammatory factors production among the supercritical fluid extracts could be used for protecting patients with atopic dermatitis from pruritus and transepidermal water loss as a functional ingredient from nature.

본 연구는 자소자 열수추출물과 압력은 일정하게 유지한 상태로 온도를 조절하여 추출한 3종의 자소자 초임계추출물에 대해 항산화력, 항균활성, biofilm 형성 억제능과 염증매개물질(nitric oxide), 염증성 사이토카인(interleukin-6, interleukin-8)의 생성 억제능을 평가하였다. 자소자 열수추출물과 비교하였을 때 자소자 초임계추출물이 항산화 효과는 떨어졌으나, Staphylococcus aureus(S. aureus)에 대한 minimal inhibitory concentration(MIC)이 월등히 낮은 것으로 확인되었고, biofilm 형성 억제능 또한 더 우수하였다. 염증매개물질과 염증성 사이토카인의 생성도 자소자 초임계추출물이 자소자 열수추출물에 비해 현저히 감소시킬 수 있었다. 따라서 우리는 초임계추출물중 가장 우수한 항균작용과 biofilm 형성 억제, 염증인자 생성 억제 효능을 보인 45℃로 추출한 자소자 초임계추출물이 아토피 피부염 환자의 가려움증과 경표피수분손실을 방지하기 위한 천연 기능성 소재로 적합함을 시사한다.

Keywords

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