• Journal of the Korean Institute of Landscape Architecture
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• v.32 no.6 s.107
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• pp.103-108
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• 2005

It was aimed to promote as a material for interior landscape by validating Rumohra aristata, in an indoor environment, especially irrigation interval, medium composition and drainage at indoor. 1. The result of physico-chemical analysis of medium composition showed that porosity, pH and Ex-Ca, Ex-Mg and Ex-K were high with peatmoss: vermiculite: perlite(1:1:1) and water contents, organic matter content, total nitrogen and cation exchange capacity were high with sand: leaf mold(1:1). 2. Growth and indoor adaptability of Rumohra aristata were better with irrigation at 2 interval per week than irrigation at 7 interval per week regardless of drainage. 3. In the case of medium composition, of growth and indoor adaptability were higher with sud: leaf mold (1:1) than peatmoss: vermiculite: perlite(1:1:1). 4. Fronds fresh weight and dry weight decreased when irrigation interval increased and were higher with sand: leaf mold(1:1) than peatmoss: vermiculite: perlite(1:1:1) treatment.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.179-185
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• 2010

This study was carried out to investigate the optimal culture conditions for gametophytes growth and sporophytes regeneration of Pyrrosia linearifolia in order to provide for the masspropagation system foundation of Pyrrosia linearifolia using their life cycle. Among many different media, 2MS medium was most effective in prothallus proliferation. Prothallus growth was promoted as the total concentration of nitrogen sources increased, and the best result was observed on 120 mM nitrogen. The best concentration of sucrose was 3%. The addition of 5~20 mM IAA, NAA, BA and kinetin promoted the propagation of prothallus. But 2iP demonstrated the most inhibitory effect on prothallus proliferation. Gametophytes shaking-cultured with liquid medium showed similar growth with solid medium and normal formation of reproductive organs. Shoot regeneration was most effective on 1/8MS medium, but growth was promoted on 1/2MS medium. For promotion of shoot regeneration and growth, the suitable concentrations of sucrose and $NaH_2PO_4$ were 1% and $50{\sim}100mg{\cdot}mL^{-1}$ in 1/8MS medium, respectively.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.5 no.2
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• pp.34-38
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• 2002

This studies were performed to determine the effects of indoor relative humidity conditions on the growth of Arachniodes aristata and Pyrrosia lingua in native fern and investigate an applicable possibility of indoor plants. The response of Arachniodes aristata and Pyrrosia lingua was evaluated in growth chamber (KG-8407-87) to 3 relative humidity and 3 soils. Relative humidity was applied to 25%, 50%, 90%; soils were prepared based on soil mixture(SM), top field soil(TF), masa soil(MS). Results of experiments are as follows ; 1. Under a low humidity condition, such as 25%, Water contents, chlorophyll content, ornamental value of Arachniodes aristata and Pyrrosia lingua decreased much more a 50-90%. 2. The growth of Arachniodes aristata were best in pots under a soil mixture(SM) but Pyrrosia lingua were field top soil(FT) under 25%. It seemed that Pyrrosia lingua due to tolerance of desiccation more than Arachniodes aristata. Though these studies, coefficient of relative humidity and soil was plays an important role in Arachniodes aristata and Pyrrosia lingua growth in indoor environments.

• Journal of Korea Foresty Energy
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• v.20 no.2
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• pp.81-116
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• 2001

The flora of vascular plants investigated in civilian control line area was 730 taxa consisting of 104 families, 331 genera, 631 species, 2 subspecies, 90 varieties, 7 formae. The forest was comparatively well conserved by Pteridophyta calculation(Pte-Q), 1.27. Among the investigated vascular plants, Korean endemic plants(Carex okamotoi, Salix koriyanagi, Clematis chisanensis, Aconitum pseudo-laeve var. erectum, Viola diamantiaca etc.) were 31 taxa; rare and endangered plants(Disporum ovale, Lilium distichum, Trillium kamschaticum, Gastrodia elata etc.) were 35 taxa; naturalized plants(Dactylis glomerata, Poa pratensis, Rumex acetosella, Gypsophila oldamiana, Lepidium apetalum etc.) were 26 taxa. The forest vegetation was classified into 1 order, 1 alliances, 6 communities; Rhododendro-Quercetalia mongolicae Kim 1990(신갈나무-철쭉군목), Lindero-Quercion mongolicae Kim 1990(신갈나무-생강나무군단), A. Carex humilis v. nana- Phinus densiflora Community 소나무-산거울군락, B. Acer pseudo-sieboldianum-Quercus mongolica Community 당단풍-신갈나무군락, C. Styrax obassia-Quercus mongolica Community 쪽동백-신갈나무군락, D. Acer palmatum-Quercus mongolica Community 단풍나무-신갈나무군락, E. Rheodendron mucronulatum-Quercus mongolica Community 진달래-신갈나무군락, F. Betula schmiditii-Quercus mongolica Community 박달나무-신갈나무군락

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.351-357
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• 2011

This study was carried out to investigate the effective extraction condition for increase of phytoecdysteroids from fronds of Matteuccia struthiopteris (FMS) and Osmunda japonica (FOJ). Lyophilizated fronds were mixed with three different solvents (MeOH, 80% EtOH or water) and then extraction was carried out by using six different methods, such as, immersion (room temp.), heating (60$^{\circ}C$), stirring (200 rpm) for 6 h, or sonication in 42 kHz ultrasonic bath for 15, 30 and 45 minutes. Contents of 20-hydroxyecdysone (20E) and ponasterone A (PonA) were measured by using HPLC after purification of the extracts by $C_{18}$ cartridge. Altogether, our results indicate that the extraction using sonication with MeOH as a solvent (for 30 minutes) was the most effective condition for 20E and PonA from both MFS and FOJ. Resulting contents of 20E from FMS and FOJ were 66.76 and 104.48 ${\mu}g{\cdot}g^{-1}$ and PonA were 53.43 and 43.82 ${\mu}g{\cdot}g^{-1}$, respectively.

• Korean Journal of Environment and Ecology
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• v.19 no.2
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• pp.162-176
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• 2005

This study was carried out as a field survey and a review of articles to investigate a characteristic of the distribution pattern in Korean endemic Corylopsis coreana. Distribution of Corylopsis coreana was found in Boseung-gun, Gwangyang-si, Suncheon-si, Jeumam-myeon Goheung-gun, Ganjeon-myeon Gurye-gun, Mt, Cheongwan Jangheung-gun, Nogodan Mt. Jiri in Jeollanam-do, Mt. Cheonhwang Namwon-si and Beamsagol and Banyabong Mt. Jiri in Jeollabuk-do, Sancheong-gun, hadong-gun and Namhea-gun in Gyeongsangnam-do and Mt. Backun, Pocheon-si in Gyeonggi-do. Total flora in the distribution region of Coryloplsis coreana was recorded as 242 taxa; 70 families, 159 genus, 216 species, 22 varieties, 4 forma. Ratio of taxonomic categories was consisted of Pteridophyte $5.8\%$, Gymnosperm $3.7\%$, Dicotyledon $18.6\%$ and Monocotyledon $71.9\%$. Ratio of Life cycle styles was Annual $1.7\%$ and Perennial $93.3\%$. Ratio of growth habit was appeared to Herb $44.2\%$, Vine $12.4\%$, Shrub $17.8\%$ and Tree $25.6\%$. The distribution region of Corylopsis coreana found to typical type which distribute in N, NE or NW slope. The native Corylopsis coreana of Sinjeonri Sungju-eup in Jellanam-do appeared to have a declining tendency which was caused by influence of tree lager.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.36 no.1
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• pp.71-77
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• 2010

The water fern Azolla belongs to the Azollaceae and forms a symbiotic association with a $N_2$-fixing cyanobacterium, referred to as Anabaena azollae, and this association has currently been demonstrated to have potential as a nitrogen source for rice production. Because of that, Azolla fern has been used not only as organic manure in southern China and northern Vietnam for a long time but also as food for animate creatures in the underwater and decontaminant in the water. However, the phenolic compounds and active materials of Azolla have not been examined in detail in the past studies. In the present study, anti-oxidant ability test and experiment to find a particular active material of Azolla imbricata and Azolla imbricata fraction (AIF) were performed. In anti-oxidant test such as DPPH test and lipoxygenase inhibition test, the value of test represented high activities compared with authentic sample - green tea and NDGA (nordihydroguaiaretic acid). In MMP-1 test, related to collagen protection and elasticity of skin, its inhibitory effect was measured over 75 %, and the phenolic compounds of AIF related with this activity were confirmed luteolin derivatives by using FT-IR spectroscopy, element analyzer (EA) and Liquid chromatography-MASS spectroscopy.

• Applied Microscopy
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• v.38 no.2
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• pp.117-124
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• 2008

Salvinia is an aquatic plant forming dimorphic leaves that have been modified into floating and submerged leaves. A air of floating leaves plays an important role for the floating and photosynthesis while the submerged leaves, which are lim and long, have the form and function of root. Many aquatic plants develop trichomes in the epidermis but in Salvinia, richomes grow densely in the epidermis of the dimorphic leaves. The present study examined the differentiation pattern of trichomes developing in the floating leaves of S. natans and S. molesta by scanning and transmission electron microscopy. Trichomes developing in the floating leaves of Salvinia showed very different patterns. In S. natans, they were arranged in a V-shape form, having 20${\sim}$25 rows at $18{\sim}25^{\circ}$ on both sides of the lamina divided by the midrib in the floating leaf. In each row, 8${\sim}$10 oval-shaped cells, $200{\sim}290{\mu}m$ in length, were arranged in a spiral fashion. Four trichomes of this form made a trichome unit, but their apical parts were separated from one another and developed into the so-called 'knuckle-crane' type. On the other hand, in S. molesta, trichomes differentiated in a unique pattern quite different from those of S. natans. At the early stage of differentiation, trichomes protruded from the epidermis and then 4${\sim}$6 cylindrical cells grew $400{\sim}600{\mu}m$ long and the four trichomes formed as an unit. The four grouped trichomes were interconnected through their apex and developed in the 'egg-beater' type. Then $300{\sim}600{\mu}m$ long multi-cellular stalk cells grew and protruded out of the epidermal surface from the basal part of the trichomes. Such a structural characteristic of trichomes is considered to play a very important role along with the aerenchyma tissue in the leaf mesophyll tissue for the floating of Salvinia on the water surface.

• Journal of Food Hygiene and Safety
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• v.29 no.3
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• pp.248-251
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• 2014

Dryopteris crassirhizoma is one of the naturally occurring substance wood ferns and is known for having anti-inflammatory, antiviral and anthelmintic activities. However, there is less report about its anticancer effect in human cancer cell lines. In the present study, the effect of methanol extract of dryopteris crassirhizoma (MEDC) on apoptosis in human oral cancer cell lines (MC3 and HN22 cells) was investigated. MEDC inhibited cell viability and induced apoptosis. MEDC significantly increased Bak and truncated Bid proteins in MC3 cells and elevated only truncated Bid compared to the control while other Bcl-2 family proteins were not altered. MEDC has anticancer activity by inducing apoptotic cell death through the regulation of either Bak or Bid. These findings suggest that its extract possibly may be used for treating oral cancer.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.38 no.1
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• pp.91-101
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• 2012

This study's purpose is to research the antioxidant power of Calaguala (Polypodium leucotomos, PL) as an oral supplement: Calaguala, a native of Central America, is a fern which belongs to the family polypodiaceae protecting the skin from the ultraviolet and active oxygen free radicals. The total 14 volunteers, each female 7 & male 7, were divided into 2 groups, then exposed to UV for 30 min after taking Calaguala and placebo. The total antioxidant capacity (TAC) was analyzed by taking the blood samples from anticubital vein 3 hours after UV exposure. First, Calaguala siginifcantly decreased the concentration of active oxygen free radicals 4 weeks later. The concentration of active oxygen free radical in PL group declined more than placebo group, when measured 30 min and 3 hours after UV exposure. Second, Calaguala did not significantly increase TAC 4 weeks later, but significantly decreased TAC 3 hours after exposure, when compared with placebo group. Now days, the photo allergy and photo aging caused by air pollution and ozone destruction have drastically increased, and thus the amount of UV radiation reaching the earth's surface increased. The increase of active free oxygen radicals resulted in accelerating the diseases and aging. The first superficial protecting protection by reflection & dispersion and the second body internal protection by taking oral photo antioxidant such as Calaguala should be continuously researched.