• Journal of Acupuncture Research
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• v.20 no.5
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• pp.236-243
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• 2003

Objective: This study is performed for the purpose of observing the effect of JSD on the cough Methods: 35 patients with the cough treated with JSD herbal acupuncture at 肺兪穴(BL13) and 平喘穴, then self-conscious complain rates were studied. Results: The result as follows. 1. There are remarkable improvement in self-conscious complain rate about chronic cough, sputum, sneezing, runny nose, sore throat, shortness of breath which chronic cough patients complained. 2. There is no significance in self-conscious complain rate according to suffering and treatment period. 3. These result indicates that the effect of JSD herbal acupuncture on chronic cough si exellent.

• Journal of Acupuncture Research
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• v.19 no.3
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• pp.156-167
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• 2002

The present study was carried out to determine if Scutellaria balicalensis Georgi. extract (SBGE) exerts beneficial effect against the renal failure in rabbits. Rabbits were pretreated with SBGE orally for 7 days, followed by cisplatin injection (5 mg/kg, i.p.), cisplatin injection caused an increase in serum creatinine level, which was accompanied by a reduction in GFR. The fractional excretion of Na+, K+, glucose, and phosphate increased in cisplatin-treated animals, which was prevented by SBGE pretreatment. The uptake of p-aminohippurate, an organic anion, in renal cortical slices was inhibited by the administration of cisplatin, and the effect was prevented by SBGE. cisplatin injection increased lipid peroxidation, which was prevented by SBGE pretreatment. These results indicate that lipid peroxidation plays a critical role in cisplatin-induced acute renal failure. SBGE exerts the protective effect against acute renal failure induced by cisplatin, and its effect may be attributed to an antioxidant action.

• Journal of Pharmacopuncture
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• v.2 no.1 s.2
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• pp.13-25
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• 1999

This study was undertaken to determine whether Juglandis Semen (JAS) extraction exerts protective effect against oxidant-induced inhibition of $Na^+$-pump activity in cerebral cortex. $Na^+$-pump activity was estimated by measuring ouabain-sensitive oxygen consumption. The oxygen consumption significantly inhibited by 1 mM t-butylhydroperoxide (tBHP), which was prevented by addition of 2% JAS extraction. The oxygen consumption was increased by an increase in $Na^+$ concentration from 5 to 100mM, $K^+$ concentration from 0.5 to 10 mM, and $Mg^{++}$ concentration from 0.2 to 5 mM. These changes in ion concentrations did not affect the inhibitory effct of tBHP and protective action of JAS on oxygen consumption. tBHP(1mM) produced a significant increase in lipid peroxidation in cerebral cortex, which was prevented by 2% JAS extraction. These result suggest that JAS exerts protective effect against tBHP-induced inhibition of $Na^+$-pump activity in the cerebral cortex, this effect may be due to by an antioxidant action.

• Journal of Pharmacopuncture
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• v.4 no.3
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• pp.69-83
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• 2001

This experiment was carried out to study on the safety assessment of Bas tures . Ursus thibetanus extract solution(BU) for Herbal acupuncture. SD rats and ICR mice were used for acute toxicity test the results were summerized as follows; 1. In rats and mice, LD50 value could not be measured. 2. There were no abnormal finding in acute toxicity test treated BU for Herbal-acupuncture.

• Journal of Pharmacopuncture
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• v.13 no.1
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• pp.45-52
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• 2010

Objectives : This experimental study was performed to investigate the effectiveness of Fel Ursi Pharmacopunture solution(FUPS) manufactured by using alcohol/water extraction method. To identify the use of it as eyedrops, antibacterial test on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, and Candida albicans was performed. Methods : After treatment FUPS on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis, we investigated anti-bacterial effects of FUPS on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans in terms of measuring MIC and size of inhibition zone respectively. Results : After FUPS was treated, significant changes of MIC on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans were not observed at all. Conclusions : The present study suggests that FUPS doesn't have anti-bacterial effects on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans which cause Keratitis. Perhap These results recommend that FUPS doesn't have anti-bacterial effects but have other mechanism which suppress inflammation.

• The Journal of Korean Medicine
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• v.20 no.2
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• pp.54-62
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• 1999

This experiment was carried out to study the safe utilization and standardization Whangryunhaedok-tang extract solution for herbopuncture on stroke patients. The test material was manufactured by water-alcohol method. The components of Whangryunhaedok-tang extract solution for herbopuncture were analyzed by HPLC. Mice were used for acute toxicity tests. The results were as follows. 1. In the berberine contents(%) of Whangryunhaedok-tang extract, berberine was 0.25%. 2. There were no abnormal findings in acute toxicity test treated with Whangryunhaedok-tang extract solution for herbopuncture and LD50 was not reached because no experimental animals were found dead throughout the study.

• Journal of Pharmacopuncture
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• v.7 no.3
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• pp.47-57
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• 2004

Objective : In order to study effects of ${\lceil}Persicae\;Semen{\rfloor}$ on the hyperlipidemia rats induced by Triton WR, we have made hyperlipidemia rats by administering Triton WR-1339 for 3 days, then have administered. The results were summerized as follows : 1. The Total cholesterol, triglyceride, LDL-cholesterol, Free fatty acid, phospholipid and CRF(Cardiak Risk Factor) by hypercholesteremic rats injected by Triton WR-1339 of the herb-Acupuncture group were decreased as compared with those of the control group, especialy hypercholesteremic rats injected by Triton WR- 1339 of the herb-Acupuncture+Acupuncture group were some more decreased as compared with those of the control group. 2. The HDL-cholesterol by hypercholesteremic rats injected by Trion WR -1339 of the herb-Acupuncture group were increased as compeared those of control group, especialy hypercholesteremic rats injected by Triton WR-1339 of the herb-Acupuncture+Acupuncture group were some more increased as compared with those of the control group. According to the above results, Persicae Semen showed inhibition on decrease of serum total cholesterol, triglyceride and phosholipid contents as well as inhibition on increase of HDL-cholesterol contents in hyperlipidemia.

• Journal of Pharmacopuncture
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• v.12 no.3
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• pp.41-47
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• 2009

Objectives : This experimental study was performed to investigate the efficacy of Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods : After administering Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis, MIC(Minimum Inhibition Concentration) and the size of inhibition zone were measured. Anti-bacterial potency was also measured using the size of inhibition zone. Results : There was no response to MIC on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) after Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution was medicated. Conclusions : The present study suggests that Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution dosen't have anti-bacterial effects on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis. These study result recommends that more research on other herbal medicines of eye drop for Keratitis are required.

• Journal of Acupuncture Research
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• v.20 no.4
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• pp.209-219
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• 2003

This study was performed to determine if Juglandis semen extract(JSE) has free radical scavenging and antioxidant activities. Superoxide anion generation by xanthine oxidase/xanthine and in neutrophils activated by phorbol-12, 13-dibutyrate was inhibited by JSE and its effect was dose-dependent. JSE also inhibited generation of $H_2O_2$ induced by glucose oxidase/glucose and in opossum kidney cells treated with antimycin A. JSE exerted a direct $H_2O_2$ scavenging effect. Exposure of opossum kidney cells to 1mM tBHP caused a significant increase in lipid peroxidation, which was prevented by JSE. JSE also prevented tBHP-induced LDH release. These data suggest that JSE has free radical scavenging and antioxidant activities. However, further studies should be carried out to find the active ingredient(s) of JSE that exerts radical scavenging action.

• Journal of Acupuncture Research
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• v.20 no.2
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• pp.184-194
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• 2003

Objective : This study was designed fo find out whether Astragali Radix Herbal-Acupuncture Solution(ARHA) can scavenge NO, DPPH and IL-4 or not. Astragali Radix has been clinically used to reforce the depression of immune reponse etc. in Oriental Medicine. Methods : Free radical metabolism seems to occupy a remarkably common position in the mechanisms of ageing and ageing related disease. Oxidative damage to DNA, lipids, proteins and other molecules may contribute to the development of cancer, cardiovascular disease and possibly neurodegenerative disease. The effects of ARHA on NO, DPPH and IL-4 were measured. Results : The followings are the summary of the result; (1) There is a significant scavenging effect of ARHA on NO in 1, $10mg/m{\ell}$ group in after 24hrs. (2) There is no significant scavenging effect of ARHA on DPPH. (3) There is a significant scavenging effect of ARHA on IL-4 in 1, $10,100{\mu}g/m{\ell}$ group. Conclusions : These results suggest that ARHA has significant scavenging effect on NO, IL-4 and not on DPPH. This study shows that ARHA can be used for ageing related disease and further studies are required to investigate the antioxidative effects of it.