• Journal of the Korean Society of Food Science and Nutrition
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• 제33권5호
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• pp.769-777
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• 2004

A Helicobacter pylori urease inhibitor from Rubus coreanus Miquel has been isolated and partially characterized for aiming to Prevent H. pylori growth and decrease harmful accumulation of ammonia in human gastric mucosa. We screened urease inhibitory activities in 519 extracts library prepared by solvent extraction from 173 kinds of edible plants, medicinal herbs, herbs and seaweeds using a colorimetric urease assay system. As results of primary and secondary screening, 70% acetone extract of Rubus coreanus Miquel was selected as potent candidate, showing about 24% inhibitory activity. The acetone extract was sequentially partitioned into RCE/RCWI and RCB/RCW2 layers with ethyl acetate and butanol. The major active component in RCW2, water layer from butanol fractionation was revealed to be peptidic or proteinous substance by inhibitory activity determination after pronase digestion and periodate oxidation. RCW2-IIIc a was isolated by sequential column chromatography on DEAE-Toyopearl 650C, Butrl-Toyopearl 650M and Sephadex LH-20. The isolated urease inhibitor RCW2-IIIc $\alpha$, was highly pure proteinous substance with molecular weight of 13kDa by high-performance gel permeation liquid chromatography. RCW2-IIIc$\alpha$ has about 5 times higher inhibitory activity than 70％ acetone extract, showing high stability against heat treatment and peptic digestion.

• FLOWER RESEARCH JOURNAL
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• 제16권1호
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• pp.23-27
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• 2008

In this experiment, growth and yield were affected by planting density, pinching and mowing dates. The greatest growth and yield were obtained at a planting density of $90cm{\times}30cm$. In this planting density, plant grew to the height of 140 cm, with 32 primary, 164 secondary, and 367 tertiary branches per plant. Weight of dry flowers reached 98 kg per 10a with this planting density, which was 40% increased as compared to planting density of $120cm{\times}30cm$. Stem diameter, shoot dry weight, and weight of dry flowers increased by pinching plants as compared to those of non-pinched plants. The greatest weight of dry flowers was obtained in the plant pinched on July 10 with 102 kg per 10a, a 57% increase as compared to the control. Growth and yield were similar for both palnts which were not mowed and mowed on June 10. However, plants mowed on July 10 had significantly smaller plant height, stem diameter and number of branches than the control. An accumulated shoot dry weight was similar for all mowing treatments. The greatest weight of dry flowers and number of flowers per plant were obtained in June 10 mowing treatment with 123 kg and 2,592 flowers per 10a, respectively.

• Korean Journal of Plant Resources
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• 제20권5호
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• pp.466-470
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• 2007

Nowadays burcucumber(Sicyos angulatus L.) has been widely used as a rootstock of Cucurbitaceae. The study was done to examine the effects of shading with different colored fabrics(white, red and aluminum-coated) on growth and cotyledon chlorophyll content of its seedling. Shading with the white colored fabric lengthened seedling height and hypocotyl length, but shading with the red colored fabric enlarged true leaf area. Total dry weight of each seedling was decreased in the order of no shading, white, red and aluminum, in which resulted from reductions of cotyledon dry weight in white colored fabric, cotyledon and true leaf dry weight in red colored fabric, and dry weight of cotyledon, true leaf and root in aluminum colored fabric. Cotyledon chlorophyll content was reduced by shading treatments although the 3 shading treatments showed nearly same content.

• Korean Journal of Plant Resources
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• 제20권5호
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• pp.446-450
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• 2007

Tiarella polyphylla D. Don(Saxifragaceae) is a perennial herb and distributed in China, Japan, Taiwan and Korea. Especially, it only grows in Ulleung island of Korea. It has been using for asthma, bruise and audition troubles with main components of some Triterpenoids and seven oleanolic Saponins. There is only known its chromosomal number rarely and cytogenetic study was not done. From this study, karyotype analysis and chromosomal localization of 5S and 45S rDNAs using bicolor-FISH(fluorescence in situ hybridization) were carried out. The somatic metaphase chromosome number was 2n=2x=14 and the size of chromosomes ranged $1.66{\sim}3.50{\mu}m$. The chromosome complement consisted of four pairs of submetacentrics(chromosomes 1, 2, 3 and 6), two pairs of subtelocentrics(chromosomes 5 and 7) and one pair of telocentrics(chromosome 4). We also observed NOR(nucleolus organizer region) on the chromosome 4. In bicolor-FISH, one pair of 55 and 45S rDNA sites was detected on the centromeric region of chromosome 3 and short arm of chromosome 4, respectively. Bicolor FISH was very useful tool for the localization and identification of rDNAs on the chromosomes in Tiarella polyphylla.

• Korean Journal of Plant Resources
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• 제23권2호
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• pp.123-130
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• 2010

Present studies were conducted to investigate the effects of autoclaving on antioxidant activities of Achillea alpina and Solodago virgurea. At early April, young leaves of 2 species were collected, subjected to autoclaving ($121^{\circ}C$, 1.2 atmospheric pressure, 15 minutes), freezed-dried, grinded, and extracted with 80% ethanol. The same process was repeated with unautoclaved control. Total polyphenol and flavonoid contents, scavenging activities on DPPH and ABTS radicals and ferrous ion chelating effects were analyzed. Extraction yield of autoclaved S. virgurea was 39.55% and A. alpina 28.15%. In both species, autoclaving significantly reduced scavenging activities on DPPH and ABTS radicals. On the contrary, ferrous ion chelating effects increased after autoclaving, especially in young leaves of A. alpina. Autoclaving resulted in decrease of polyphenol and flavonoid contents, especially in the A. alpina. The present experiments demonstrated that autoclaving had negative effects on antioxidant activities of A. alpina and S. virgurea, except in ferrous ion chelating effects. Young leaves of former species were unstable to heat treatment, resulting in big lose of antioxidant activity.

• Journal of Applied Biological Chemistry
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• 제64권3호
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• pp.245-251
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• 2021

Codonopsis lanceolata (Deoduk) was grown in East Asia, including Korea, China, Japan, and Russia, and the roots of C. lanceolata have been used as functional foods and traditional medicine to treat symptoms of cough, bronchitis, asthma, tuberculosis, and dyspepsia. The phytochemicals of C. lanceolata have been reported such as phenylpropanoids, polyacetylenes, saponins, and flavonoids that are involved in pharmacological effects such as anti-obesity, anti-inflammation, anti-tumor, anti-oxidant, and anti-microbial activities. Selecting marker substances of the main producing area by MS-based metabolomics analysis is important to ensure the beneficial effect of C. lanceolata without side-effects because differences in cultivated areas of plants were related not only to the safety of medicinal plants but also to changes in chemical composition and biological efficacy. In our present study, ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry combined with multivariate statistical analysis was applied to recognize the main producing area of C. lanceolata in South Korea. As a result of Principal Component Analysis and loading plot analysis of three groups, Inje (Kangwon-do), Hoengseong (Kangwon-do), and Muju (Jeonlabuk-do), several secondary metabolites of C. lanceolata including tangshenoside I, lancemaside A, and lancemaside G, were suggested as potential marker substances to distinguish the place of main producing area of C. lanceolata.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제22권5호
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• pp.379-386
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• 2021

This study was undertaken to develop a cascade-type continuous culture system (CCCS) that combines both ICT and biotechnology (BT), for the mass production of microalgae. This system is capable of maintaining the essential culture conditions of pH, temperature, carbon dioxide, and illuminance control, which are key parameters for the growth of microalgae, and is economical for producing microalgae regardless of the season or location. It has the added advantage of providing stable and high productivity. In the current study, this system was applied to culture microalgae for 71 days, with subsequent analysis of the experimental data. The initial O.D. of the culture measured from incubator 1 was 0.006. On the 71st day of culture, the O.D.s obtained were 0.399 (incubator 1), 0.961 (incubator 2), 0.795 (incubator 3), and 0.438 (incubator 4), thereby confirming the establishment of continuous culture. Thus, we present a smart-farm based on ISMC (in-situ monitoring and control) for a mass culture method. We believe that this developed technology is suitable for commercialization, and has the potential to be applied to hydroponics-based cultivation of microalgae and cultivation of high-value-added medicinal plants as well as other plants used in functional foods, cosmetics, and medical materials.

• Korean Journal of Plant Resources
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• 제32권1호
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• pp.45-52
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• 2019

In the process of adapting climate change, the government needs to provide policy and technical support necessary for growing promising crops imported from abroad. Therefore, this study was conducted to survey and analyze the conditions of growth of imported foreign crops and to derive response tasks. As a result, tropical and subtropical vegetables were cultivated 18 crops in 920 farms in 321 ha area (in 2015 year). The cultivation scale decreased in the order of Curcuma aromatica, Momordica charantia, Asparagus aethiopicus, Allium hookeri and Herbs. Tropical and subtropical fruits were cultivated 9 crops in 264 farms in 106.5 ha area (in 2015 year). Special and medicinal crops introduced abroad cultivated 10 crops in 753 farms in 276.3 ha area (in 2015 year). The cultivation scale decreased in the order of Curcuma longa, Glycyrrhiza uralensis, Lepidium meyenii and Moringa oleifera. For the stable settlement of domestic growth of tropical and subtropical crops introduced abroad, there should be safety and economic feasibility in terms of the cultivation environment according to the domestic culture adaptation test. Consideration needs to be given to the use of locally grown products in Korea, the securing of distribution and sales markets, and the competitiveness of imported products.

• Journal of Ginseng Culture
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• 제1권
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• pp.28-42
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• 2019

What is unknown about Leibniz (Gottfried Wilhelm Leibniz, 1646~1716), a great philosopher and mathematician, is that he inquired about ginseng. Why Leibniz, one of the leading figures of the Enlightenment, became interested in ginseng? This paper excavates Leibniz's references on ginseng in his vast amount of correspondences and traces the path of his personal life and cultural context where the question about ginseng arose. From the sixteenth century, Europe saw a notable growth of medical botany, due to the rediscovery of such Greek-texts as Materia Medica and the introduction of a variety of new plants from the New World. In the same context, ginseng, the renowned panacea of the Old World began to appear in a number of European travelogues. As an important part of mercantilistic projects, major scientific academies in Europe embarked on the researches of valuable foreign plants including ginseng. Leibniz visited such scientific academies as the Royal Society in London and $Acad{\acute{e}}mie$ royale des sciences in Paris, and envisioned to establish such scientific society in Germany. When Leibniz visited Rome, he began to form a close relationship with Jesuit missionaries. That opportunity amplified his intellectual curiosity about China and China's famous medicine, ginseng. He inquired about the properties of ginseng to Grimaldi and Bouvet who were the main figures in Jesuit China mission. This article demonstrates ginseng, the unnoticed subject in the Enlightenment, could be an important clue that interweaves the academic landscape, the interactions among the intellectuals, and the mercantilistic expansion of Europe in the late 17th century.

• Journal of Applied Biological Chemistry
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• 제64권2호
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• pp.171-176
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• 2021

Aloe has been widely used as a cosmetic and medicinal plant. Until now, several effects such as antioxidant, anti-cancer, anti-diabetic, immunity and whitening of aloe gel extract have been reported, but research on aloe by-products occurring in food processing has not been actively conducted. In this study, we investigated whether the aloe by-product extract from food processing could be used as a functional biomaterial. Cytotoxicity was not seen in both the mixer and press extracts. Inhibition of 3T3-L1 adipocyte differentiation was detected only in the mixer extract and not in the press. It was confirmed that hyaluronic acid accumulation and tyrosinase inhibition increased according to the treatment concentration of the mixer extract. The antimicrobial activity of the mixer extract was observed in the Porphyromonas gingivalis strain, but not in the Streptococcus mutans strain. Antioxidant activity through DPPH and SOD analysis increased with the concentration of the mixer extract. In summary, it was confirmed that the mixer extract of aloe by-products has the effect of inhibiting adipocyte differentiation, moisturizing, whitening, and antioxidant, suggesting the possibility of using it as a functional bio-material for health drinks or beauty masks.