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http://dx.doi.org/10.3746/jkfn.2004.33.5.769

Isolation and Characterization of Helicobacter pylori Urease Inhibitor from Rubus coreanus Miquel  

양성우 (고려대학교 생명공학원)
호진녕 (고려대학교 생명공학)
이유현 (고려대학교 생명공학)
신동훈 (고려대학교 생명공학)
홍범식 (고려대학교 생명공학)
조홍연 (고려대학교 생명공학원)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.5, 2004 , pp. 769-777 More about this Journal
Abstract
A Helicobacter pylori urease inhibitor from Rubus coreanus Miquel has been isolated and partially characterized for aiming to Prevent H. pylori growth and decrease harmful accumulation of ammonia in human gastric mucosa. We screened urease inhibitory activities in 519 extracts library prepared by solvent extraction from 173 kinds of edible plants, medicinal herbs, herbs and seaweeds using a colorimetric urease assay system. As results of primary and secondary screening, 70% acetone extract of Rubus coreanus Miquel was selected as potent candidate, showing about 24% inhibitory activity. The acetone extract was sequentially partitioned into RCE/RCWI and RCB/RCW2 layers with ethyl acetate and butanol. The major active component in RCW2, water layer from butanol fractionation was revealed to be peptidic or proteinous substance by inhibitory activity determination after pronase digestion and periodate oxidation. RCW2-IIIc a was isolated by sequential column chromatography on DEAE-Toyopearl 650C, Butrl-Toyopearl 650M and Sephadex LH-20. The isolated urease inhibitor RCW2-IIIc $\alpha$, was highly pure proteinous substance with molecular weight of 13kDa by high-performance gel permeation liquid chromatography. RCW2-IIIc$\alpha$ has about 5 times higher inhibitory activity than 70％ acetone extract, showing high stability against heat treatment and peptic digestion.
Keywords
Helicobacter Pylori; Rubus coreanus Miquel; urease inhibitor;
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