• Weed & Turfgrass Science
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• v.2 no.1
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• pp.70-75
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• 2013

Seven medicinal plant extracts were tested for antifungal activities against six species of the major turfgrass pathogenic fungi (Colletotrichum graminicola, Pythium spp., Rhizoctonia cerealis, Rhizoctonia solani AG1-1, Rhizoctonia solani AG2-2, and Sclerotinia homoeocarpa) using paper disk diffusion method. Three medicinal plant extracts, including Pinus densiflora showed antifungal activities. In suppression of mycelium growth test, on medium adding P. densiflora extract showed that inhibition rate of mycelium growth were above 80% in 10 mg/10 ml concentration of the extract. The inhibition rate of Pythium spp. was 100% and C. graminicola was 84.3% in 10 mg/10 ml concentrations of P. densiflora extract, respectively. In particularly, the inhibition rate of Pythium spp. was 89.5% in 2 mg/10 ml concentrations of P. densiflora extract. As a result, P. densiflora extract showed high antifungal activity to Pythium spp. and C. graminicola of the turfgrass pathogen in in vitro test.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.4
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• pp.506-510
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• 2010

As an attempt to develop new functional health beverage by using medicinal herb, we investigated the effect of medicinal plant extract (MPE) on mean arterial blood pressure (MABP) and regional cerebral blood flow (rCBF) of rats. The changes of MABP and rCBF were determined by LDF methods. LDF allows for real time, noninvasive, continuous recordings of local CBF. MABP in MPE treated rats showed significant change of MPE 1.0 and 10.0 mg/kg. MPE i.v. administration showed significant increase of rCBF in a dose-dependent manner. Propranolol pretreated MABP showed significant change in the increase of MPE. rCBF of propranolol pretreated rats showed significant change from the i.v. injection concentration of 1.0 and 10.0 mg/kg. The ischemia/reperfusion induced oxidative stress may have contributed to cerebral damage in rats, and the present study provides clear evidence for the beneficial effect of MPE on ischemia induced brain injury. Also, the action mechanism in elevation effect of MPE on rCBF might be concerned with the role of $\beta$-adrenoceptor. The exact component and mechanism remains for the future study.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.4
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• pp.512-519
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• 2013

The antioxidant activities of ethanolic extracts obtained from medicinal plants (Scutellaria baicalensis Georgi, Acanthopanax sessiliflorum Seeman, Pueraria lobata Ohwi, Portulaca oleracea Linne, Crataegus pinnatifida Bunge var. typica Schneider, Euonymus alatus Apterus, Hovenia dulcis Thunberg, Prunus yedoensis Matsumura, Albizzia julibrissin Durazz., Chrysanthemum indicum Linne) were evaluated for total phenolic content, total flavonoid content, DPPH radicals, nitrites, $Superoxide^-$ radicals, $ABTS^+$ radical scavenging activity, and reducing power. Antioxidant capacities were the highest in Prunus yedoensis Matsumura for DPPH radical scavenging activity ($IC_{50}$ $5.39{\mu}g/mL$), reducing power (2.72, $A_{700}$), and nitrite scavenging activity ($IC_{50}$ $167.94{\mu}g/mL$). Hovenia dulcis Thunberg and Acanthopanax sessiliflorum Seeman were effective for their nitrite scavenging activities (over 90% at 1 mg/mL). The $Superoxide^-$ radical scavenging activity of Prunus yedoensis Matsumura ($IC_{50}$ $43.39{\mu}g/mL$) was stronger than tannic acid ($IC_{50}$ $46.51{\mu}g/mL$). Five samples (Prunus yedoensis Matsumura, Acanthopanax sessiliflorum Seeman, Hovenia dulcis Thunberg, Crataegus pinnatifida Bunge var. typica Schneider, Scutellaria baicalensis Georgi) were effective for their $ABTS^+$ radical scavenging activity (more than 90% at 0.5 mg/mL). These results suggest that the ethanolic extracts of Prunus yedoensis Matsumura could be used as a functional ingredient in food products.

• Journal of Life Science
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• v.21 no.4
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• pp.596-603
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• 2011

This study was conducted to investigate traditional medicinal plants (TMP) 1 and 2, two different multi-herbal mixtures consisting of 24 herbs. Regarding the contents of flavonoid compounds, the ethanol extract (EE) of TMP2 yielded the highest content of flavonoid compounds (40.981 mg/g), followed by EE of TMP1 (28.23 mg/g), hot water extract of TMP2 (WE, 10.046 mg/g), and WE of TMP1 (6.59 mg/g). Antioxidant activities of EE and WE of TMP1 and TMP2 were measured based on DPPH radical scavenging activity. At 1 mg/ml, the highest DPPH radical scavenging activity was shown in EE of TMP2 (96%), followed by EE of TMP1 (94%). Nitric oxide (NO) production by RAW264.7 macrophage cells stimulated with lipopolysaccharide (LPS) was reduced to 82, 77, 86, and 47% by addition of $500\;{\mu}g/ml$ of EE and WE of TMP1 and TMP2, respectively. These results were not due to the cytotoxicity of the extracts. NO synthesis was increased by 2.3, 3.6, 3.0, and 1.8 fold by addition of $500\;{\mu}g/ml$ of EE and WE of TMP1 and TMP2, respectively. These results show that medicinal plants play a significant role in antioxidative activity and activation of the immune system in the pathogenesis of inflammatory diseases, and different mixtures of the medicinal plants showed different effects.

• 한국약용작물학회:학술대회논문집
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• 2007.05a
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• pp.107-108
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• 2007

• 한국약용작물학회:학술대회논문집
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• 2007.11a
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• pp.308.1-308.1
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• 2007

• Journal of Animal Science and Technology
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• v.52 no.5
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• pp.383-388
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• 2010

Identification of natural compounds that can prevent the development of obesity in vivo is time consuming and expensive. We have used in vitro systems derived from pig adipose tissue to screen simple aqueous or ethanolic extracts of Korean medicinal herbs (KMH) for their anti-adipogenic potential. A total of 183 extracts were tested for their actions in lipogenesis of pig adipose tissue and differentiation of pig preadipocytes. Ethanol extracts were prepared from 72 and aqueous extracts were prepared from 111 medicinal herbs. Both an ethanolic and an aqueous extract were prepared from 65 of these. Thirteen extracts substantially altered rates of lipogenesis in vitro. The effects of KMH on lipogenesis of pig adipose tissue are as follows. Elevens reduced lipogenesis to rates that were more than 40% lower than control and four of these reduced rates of lipogenesis by more than 70%. The most potent anti-lipogenic extracts were those obtained in ethanol from Iridaceae and from Sophora flavescens AIT as well as both the aqueous and ethanolic extracts from Lysimachia vulgaris L. Two extracts, those prepared in water from Caesalpiniae lignum and from Phellodendri cortex, were found to promote rates of lipogenesis in vitro. The effects of KMH on differentiation of pig preadoipocytes are as follows. Twentyeight extracts altered the rates of differentiation of cultured porcine preadipocytes. Sixteen increased and twelve reduced the rates of differentiation of preadipocytes. Extracts prepared in ethanol from Moutan radicis cortex and from Ostericum koreanum and those prepared in water from Angelicae gigantis radix, from Inula henenium L and from Magnolia flos doubled the rate of differentiation of cultured porcine preadipocytes. Ten extracts reduced the in vitro rate of differentiation of porcine preadipocytes by more than 35%. These were the ethanolic extracts from Glycyrrizae radix, Nepetae spica and from Polygala myrtifolia and the aqueous extracts from Amaranthaceae, Asparagus cochinchinesis, Atractylodis rhizoma alba, Citrus junos TANAKA, Cyperus rotundus, Epimedium grandiflorum and from Moutan radicis cortex. Only the ethanolic extract from Polygala myrtifolia was able to both reduce lipogenesis in adipose tissue slices and retard differentiation of cultured preadipocytes. The results of our study will provide meaningful information to identify medicinal herbs which would reduce fat deposition in livestocks and humans.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.2 s.57
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• pp.105-110
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• 2006

This study was to describe the differences in efficacy and effect of herbal extracts by the part and solvent extraction from the medical plants used as materials of oriental herbs cosmetics. And, this study was to apply to the test method of efficacy and effect related to the antioxidation as herbal extracts, complex of actual ingredient, not existing analytical methods of single ingredient. After screening the medical plants with the antioxidative activity primarily and selecting 11 sorts of medical plants to be used by the part in the literature, this study was to confirm the differences through the well-known test methods like DPPH radical scavenging activity test and hydroxyl radical scavenging activity test. For examples, in case of Trachelospermum asiaticum, compared with the aerial part and fruit, the value of DPPH radical scavenging activity test had $25.2 {\pm} 0.2$ and $62.4 {\pm}1.6$ each. It has shown that the value of fruit had 2.4 times higher effect than the one of aerial part. In case of hydroxyl scavenging activity test, it was effective in the fruit, but it has shown that there was no effect on the aerial part. It showed the same phenomena in some other plants. From the result above, this researcher could understand that it needed to consider extracting the medical plants or plants with the active principle by the part. Also, this study was to confirm the differences in effect according to the solvent as it changed the solvent extraction after selecting a plant (Lithospermum erythrorhizon) widely used for medicine and dye. As a result of measuring the actual value of superoxide scavenging activity test, this study was to consider that there were differences by the part or solvent extraction in extracting and using the medical plants as it has shown that the effect differences produced $10{\sim}80%$ according to the solvent. When it was applied to the products, this study has shown that it needed to decrease the possible errors.

• Journal of the East Asian Society of Dietary Life
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• v.19 no.2
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• pp.224-230
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• 2009

This study was to investigated the effects of adding of medicinal herbal (cinnamon, licorice and Bokbunja) to ground beef during storage by examining surface color, water holding capacity, cooking loss, increased rate of thickness, decreased rate of diameter, pH, VBN (volatile basic nitrogen) content and TBARS (2-thiobarbituric acid reactive substances) values of the beef under cold ($4{\pm}1$) storage conditions for 9 days. Four types of ground beef were prepared by adding the follow: 10% water (control), 10% cinnamon extracts (T-1), 10% licorice extracts (T-2), and 10% Bokbunja extracts (T-3). The $L^*$ (Lightness) and $a^*$ (Redness) values of the beef significantly decreased (p<0.05) whereas no significant changes were shown in the $b^*$ (Yellowness) value during storage, and which were not influenced by the additions of herbal extracts. Water holding capacity was significantly increased during cold storage (p<0.05), and was not influenced by the addition of the extracts. The cooking loss of the control, T-2 and T-3 were significantly decreased during cold storage (p<0.05), and T-1 had no significant changes in cooking loss. There were no significant changes in the thickness and diameter during cold storage, and which were not influenced by the addition of the extracts. The pH of the control decreased until 6 days of storage, but increased at 9 days, and the pH levels of T-1, T-2 and T-3 decreased during cold storage. VBN content was increased during cold storage and the VBN contents of the samples containing herbal extracts were lower than that of the control. In addition, the TBARS values of the ground beef containing the extracts were lower than that of the control.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.43-43
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• 2021

로즈마리(Rosmarinus officinalis)는 지중해 지역이 원산이고 꿀풀과에 속하는 다년생 식물로 자생지에서는 식물의 크기가 2m까지 자라는 관목성 식물이다. 식용, 약용, 미용, 향료뿐 아니라 관상용으로도 널리 이용되고 있고 특히 향이 좋아 세계 로즈마리 추출물 시장은 연평균 4.8%씩 증가하여 2027년에는 10억 달러를 넘을 것으로 예측된다. 우리나라도 소비 트렌드 변화에 따른 이용성 확대로 신선허브의 수요가 증가하고 있으나 아직은 허브 식물원료의 대부분을 수입에 의존하고 있고, 로즈마리 역시 식물원료뿐 아니라 가공품까지 외국에서 수입하여 사용하는 실정이다. 2018년 로즈마리 수입량은 신선상태 978kg, 건조상태 23,404kg으로 높은 수입의존에 따른 가격 상승과 긴 유통기간에 의한 품질 저하 등의 문제가 발생하고 있다. 본 연구는 로즈마리 어린 순 재배 적정 삽수 길이를 설정하고 어린 순 생산 가능 기간을 구명하여 추후에 고품질 로즈마리 어린 순 집약생산을 위한 다단재배기술을 확립하고자 수행되었다. 삽수 길이는 5, 10, 15cm로 하였고, 삽목 시기는 4월 하순 ~ 8월 하순까지 30일 간격으로 5회 실시하였다. 적정 삽수 길이 설정 실험에서는 15cm 삽수 발근률이 85.6%로 가장 높았으며 신초 출현시기는 5월 26일, 어린 순 생산시기는 6월 23일로 가장 빨랐고 수확시까지 소요일수는 56일로 가장 짧았다. 기대수량 또한 728g/m²로 가장 높았다. 로즈마리 어린 순 생산 가능 기간 구명 실험에서는 4월 28일 삽목시 발근율이 85.6%로 가장 높았고 육묘기간은 28일 어린 순 생산까지 소요일수는 56일로 가장 짧았다. 삽목 시기별 어린 순 품질 및 생산량은 4월 28일 삽목시 품질이 좋았으며 기대수량 또한 728g/m²로 가장 높았다. 결과적으로 상품성 있는 어린순 생산에 적합한 삽수 길이는 15cm, 삽목 시기는 4월 하순 경에 했을 때, 로즈마리의 생육상태, 수확까지의 기간, 어린 순 생산량 등 종합적인 면에서 가장 우수한 값을 얻을 수 있었다.