Browse > Article
http://dx.doi.org/10.5187/JAST.2010.52.5.383

Extracts of Korean Medicinal Plant Extracts Alter Lipogenesis of Pig Adipose Tissue and Differentiation of Pig Preadipocytes In vitro  

Choi, Young-Suk (Department of Animal Science, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Choi, Kang-Duk (Department of Biotechnology Hankyong National University)
Kim, Sung-Do (East West Kidney Diseases Research Institute, School of Medicine, Kyung Hee University)
Phillip, Owens (Department of Animal Science, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Chung, Chung-Soo (Department of Animal Science, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Publication Information
Journal of Animal Science and Technology / v.52, no.5, 2010 , pp. 383-388 More about this Journal
Abstract
Identification of natural compounds that can prevent the development of obesity in vivo is time consuming and expensive. We have used in vitro systems derived from pig adipose tissue to screen simple aqueous or ethanolic extracts of Korean medicinal herbs (KMH) for their anti-adipogenic potential. A total of 183 extracts were tested for their actions in lipogenesis of pig adipose tissue and differentiation of pig preadipocytes. Ethanol extracts were prepared from 72 and aqueous extracts were prepared from 111 medicinal herbs. Both an ethanolic and an aqueous extract were prepared from 65 of these. Thirteen extracts substantially altered rates of lipogenesis in vitro. The effects of KMH on lipogenesis of pig adipose tissue are as follows. Elevens reduced lipogenesis to rates that were more than 40% lower than control and four of these reduced rates of lipogenesis by more than 70%. The most potent anti-lipogenic extracts were those obtained in ethanol from Iridaceae and from Sophora flavescens AIT as well as both the aqueous and ethanolic extracts from Lysimachia vulgaris L. Two extracts, those prepared in water from Caesalpiniae lignum and from Phellodendri cortex, were found to promote rates of lipogenesis in vitro. The effects of KMH on differentiation of pig preadoipocytes are as follows. Twentyeight extracts altered the rates of differentiation of cultured porcine preadipocytes. Sixteen increased and twelve reduced the rates of differentiation of preadipocytes. Extracts prepared in ethanol from Moutan radicis cortex and from Ostericum koreanum and those prepared in water from Angelicae gigantis radix, from Inula henenium L and from Magnolia flos doubled the rate of differentiation of cultured porcine preadipocytes. Ten extracts reduced the in vitro rate of differentiation of porcine preadipocytes by more than 35%. These were the ethanolic extracts from Glycyrrizae radix, Nepetae spica and from Polygala myrtifolia and the aqueous extracts from Amaranthaceae, Asparagus cochinchinesis, Atractylodis rhizoma alba, Citrus junos TANAKA, Cyperus rotundus, Epimedium grandiflorum and from Moutan radicis cortex. Only the ethanolic extract from Polygala myrtifolia was able to both reduce lipogenesis in adipose tissue slices and retard differentiation of cultured preadipocytes. The results of our study will provide meaningful information to identify medicinal herbs which would reduce fat deposition in livestocks and humans.
Keywords
Medicinal herbs; Lipogenesis; Adipocyte differentiation; Pig;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Suryawan, A., Swanson, L. V. and Hu, C. Y. 1997. Insulin and hydrocortisone, but triiodothyronine, are required for the differentiation of pig preadipocytes in primary culture. Journal of Animal Science 75:105-111.
2 Wise, L. S. and Green, H. 1979 Participation of one isozyme of cytosolic glycerophoaphate dehydrogenase in the adipose conversion of 3T3 cells. Journal of Biological Chemistry 254:273-275.
3 Lee, C. Y., Bazer, F. W, Etherton, T. D. and Simmen, F. A. 1991. Ontogeny of insulin-like growth factors (IGF-I and IGF-II) and IGF-binding proteins in porcine serum during fetal and postnatal development. Endocrinology 128:2336-2344.   DOI   ScienceOn
4 Lee, H. Y., Kang, R. H. and Toon, Y. S. 2010. SH21B, an anti-obisity herbal composition, inhibits fat accumulation in 3T3-L1 adipocytes and high fat diet-induced obese mice through the modulation of the adipogenesis patyway. Journal of Ethnopharmacoligy 127:709-717.   DOI   ScienceOn
5 Moses, A. D., Nissley, S. P., Short, P. A., Rechler, M. M., White, R. M, Knights, A. B. and Higa, O. Z. 1980. Increased levels of multiplication-stimulating activity, an insulin-like growth factor, in fetal rat serum. Proceedings of National Academy of Science, USA 77:3649-3653.   DOI
6 Ogawa, T., Tabata, H., Katsube, T., Ohta, Y., Yamasaki, Y., Yamasaki, M. and Shiwaku, K. 2010. Supression effect of hot water extract of wasabi (Wasabia japonica Matrum.) leaves on the differentiation of 3T3-L1 preadipocytes. Food Chemistry 118239-244.
7 Owens, P. C., Gatford, K. L., Walton, P. E., Morley, W. and Campbell, R. G. 1999. The relationship between endogenous insulin-like growth factors and growth in pigs. Journal of Animal Science. 77:2098-2103.
8 Popovich, D. G., Li, L. and Zhang, W. 2010. Bitter melon (Monordica charantia) triterpenoid extract reduces preadipocyte viability, lipid accumulation and adiponectin expression in 3T3-L1 cells. Food and Chemistry Toxicology 48:1619-1626.   DOI   ScienceOn
9 Skidmore, P. M. L. and Yarnell, J. W. G. 2004. The obesity epidemic: prospects for prevention. Quarterly Journal of Medicine 97:817-825.   DOI   ScienceOn
10 Rayalam, S., Delaa-Fera, M. A. and Baile, C. A. 2008. Phytochemicals and regulatiom of the adipocytes life cycle. Journal of Nutritional Biochemistry 19:717-726.   DOI   ScienceOn
11 Adeneye, A. A., Adeyemi, O. O. and Agbaje, E. O. 2010. Antiobesity and antihyperlipidaemic effect of Hunteria umbellate seed extract in experimental hyperlipidaemia. Journal of Ethnopharmacoligy 130:307-314.   DOI   ScienceOn
12 Ahima, R. S. 2006. Obesity epidemic in need of answers. Gastroenterology. 131:991.   DOI   ScienceOn
13 Cheetham, S. C., Jackson, H. C., Vickers, S. P., Dickinson, K., Jones, R. B. and Heal, D. J. 2004. Novel targets for the treatment of obesity. Drug Discovery Today: Therapeutic Strategies 1:227-235.   DOI   ScienceOn
14 Dole, V. P. 1956. A relation between nonesterified fatty acids in plasma and the metabolism of glucose. Journal of Clinical Investigation 35:150-154.   DOI   ScienceOn
15 Dunshea, F. R., Suster, D., Kerton, D. J. and Leury, B. J. 2003. Exogenous porcine somatotropin administered to neonatal pigs at high doses can alter lifetime fat but not lean tissue deposition. British Journal of Nutrition 89:795-801.   DOI   ScienceOn
16 Huang, Y. W., Lie, Y., Dushenkov, S., Ho, C. T. and Huang, M. T. 2009. Anti-obesity effects of epigallocatechin-3-galaate, orange peel extract, black tea extract, caffeine and their combinations in a mouse model. Journal of Functional Food. 1:304-310.   DOI   ScienceOn
17 Herd, R. M., Archer, J. A. and Arthur, P. F. 2003. Reducing the cost of beef production through genetic improvement in residual feed intake: opportunity and challenges to application. Journal of Animal Science 81 (E Supplement 1):E9-E17.