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Comparison of Biotyping, Serotyping and Molecular Typing of Yersinia enterocolitica Isolated from Spring water in Seoul (서울시내 약수에서 분리한 Yersinia enterocolitica의 생물형, 혈청형 및 분자학적 형별비교)

  • 이영기;최성민;오수경;신재영
    • Journal of environmental and Sanitary engineering
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    • v.14 no.4
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    • pp.99-109
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    • 1999
  • Enteropathogenic Yersina enterocolitica is an important cause of human and animal disease. Phenotypic and genotypic characteristics currently used to identify Yersinia enterocolitica are not necessarily sufficient to differentiate pathogenic from non-pathogenic strains or to analyze the epidemiology of yersiniae at a molecular level. To improve the characterization of Yersinia enterocolitica, A total of 65 isolates of Yersinia enterocolitica were examined with bioserotyping, antibiotic susceptibilities, PFGE, PCR-ribotyping. Genomic DNA pattern generated by PFGE are highly specific for different strains of an organism and have significant value in epidemiologic investigations. The PFGE analysis of Not I-digested chromosomal DNA of Y. enterocolitica were performed with a CHEF Mapper(Bio-Rad, USA). Not I generated 19 restriction endonuclease digestion profiles(REDP). PCR-ribotyping, performed with primers complementry to conserved regions of 16S and 23S rRNA gene, generated 13 ribotypes. PCR-ribotyping can be considered a good technich for subtyping strains of Y.enterocolitica.

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A Study on Water Quality of Springs in the Suburbs of Chunchon City According to Seasons (춘천근교 약수의 계절별 수질에 관한 보건학적 조사연구)

  • 한돈희;박영의;박찬정;전병구;박갑만
    • Journal of Environmental Health Sciences
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    • v.13 no.1
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    • pp.7-16
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    • 1987
  • A study was carried out to determine the adequacy of springs as drinking water in summer and in winter separately. In this study, environmental sanitation, physio-chemical examination, bacteriologic contamination and the content of heavy metal were included. For this study, samples were collected from 8 springs which were located in the suburbs of Chunchon city. The following results were obtained. 1. There was the covering system in 1 out of 8 springs. 6 out of 8 springs showed contaminating source within lorn. 2. In physio-chemical test, six out of 8 springs were found to be unsafe for the legitimate standard of safty water. 3. Bateriologic examination clone during summer showed the evidence of coilform group at 7 out of 8 springs and during winter showed positivity at 3 out of 8 springs. 4. In the study for heavy metal content, all places showed high iron level beyond the standard level of salty water, and 4 places showed high contents of fluorine, mangan and lead.

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Mineral Water Investigation on 10 Area in Seoul (서울시내(市內) 10개(個) 약수(藥水)의 수질오염(水質汚染)에 관(關)한 연구(硏究))

  • Kim, Hyung-Suk;Koo, Do-Suh;Park, Yang-Won
    • Journal of Preventive Medicine and Public Health
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    • v.10 no.1
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    • pp.59-61
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    • 1977
  • We are calling the erupting ground water as drugwater or mineral water in Korea and thinking those water is good for health from ancient. But most of them were used by mountainer as drinking water and food making water. Authors tried to investigate the evidence of contamination by user on the 10 erupting ground water and gained following results: 1. All of the mineral, waters on 10 area in Seoul were unfitable to drinking water standard. 2. In the view of the bacteriology 80% were contaminated by coliform group. 3. The highest value of the free carbon dioxide contents were 652.96 ppm at Sam Sun Mineral Water.

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Studies on the Mineral Water in Seoul Area (서울시 일원 약수의 수질에 관한 조사연구)

  • Choi Han-Young
    • Journal of environmental and Sanitary engineering
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    • v.3 no.1 s.4
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    • pp.69-74
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    • 1988
  • This study was carried out to investigate water quality and pollution in the mineral water located in Seoul area. 15 sites were pointed out by random sampling and tested monthly for 14 items from July to December in 1986. 1. 14 sites were suitable for the drinking water standards but 1 site was not suitable for its pH (5.4). 2. The range of mineral element and concentration were as follows: $$K\;(1.1\pm0.19\~2.38\pm0.45ppm)$$ $$Na\;(5.14\pm0.32\~11.2\pm0.58ppm)$$ $$Ca\;(4.36\pm0.53\~14.44\pm0.53ppm)$$ $$Mg(1.58\pm0.32\~6.34\pm0.77ppm)$$ 3. Among the heavy metals, Fe, Zn, Mn, Cu, were detected small amount but Pb was not detected in all sample. 4. Fluoride concentrations were the range of $0.13\pm0.03\~0.42\pm0.03ppm$.

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A Study on the Side Effects of Using Herbal Medicine Assigned to a Different Constitutional Type (타약수해례(他藥受害例) 연구)

  • Yu, Jun-Sang
    • Journal of Sasang Constitutional Medicine
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    • v.21 no.2
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    • pp.115-122
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    • 2009
  • 1. Purpose : Sasang constitutional herb medicine is known as specific to his own's constitution. So Other constitutional herb medicine can induce side effects. This study investigates the cause and side effects of other constitutional herb medicine. 2. Methods : Literature study was carried out with the chapters including harm to Other constitutions in the books "Dongyisasangjinryoeuijeon(東醫四象診療醫典)" and "Joeuihak(朝醫學)". And the causes of side effects were studied in terms of herbology and movements of Qi in Sasang constitutional medicine. 3. Results : Herb medicines of Taeeumin's have the dispersing Qi energy, so Soeumin could have side effects mostly and some of Soyangin could do. Herb medicines of Soeumin's have the warming Qi energy, so Soyangin could have side effects like dermatitis or fever. Herb medicine of Soyangin's have cooling Qi energy, so Soeumin could have side effects of digestive system like diarrhea and some of Taeeumin could do. 4. Conclusion : It is thought that herb medicine is safe and less harmful when herb medicine is prescribed and taken according to his own constitution.

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Silicate- silicious of Spring Water and Ground Water in Pusan Area (부산 지역 약수 및 지하수의 규산성 규소)

  • 김용관;하봉석
    • Journal of Environmental Health Sciences
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    • v.18 no.1
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    • pp.34-38
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    • 1992
  • This study was carried out to investigate silicate-silicious in spring water and ground water. Samples were collected from 23 Stations of spring water and 2 Stations of ground water from August 1985 to July 1990 in Pusan area. Range and mean value of silicate-silicious of spring water and ground water were lower 2.12~22.70mg/l, 8.78mg/l, 13.90~19.05mg/l, 16.23mg/l than the criterion for drinking water as 50rng/l. The concentration of silicate-silicious had a great influence on precipitation, therefore it in summer was higher than in winter. The estimated regression line between chloride ion(x) and silicate-silicious(y) of the sampled water was y=19.366-0.604x(r=-0.7111).

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Antibiotic and Heavy Metal Resistance of Coliform Bacteria Isolated from Mineral Water (약수에서 分離한 大腸菌群의 일부 중금속 및 抗生劑耐性에 관한 연구)

  • Jeong, Jee-Yeon;Zong, Moon-Shik
    • Journal of Environmental Health Sciences
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    • v.15 no.1
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    • pp.63-73
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    • 1989
  • The purposes of this study were to find out the heavy metal and antibiotic resistant coliform bacteria from mineral water and the resistant factors. For the experiment, mineral water samples were taken from A area and B area during the period from march to July, 1988. The results of the experiment were as follows 1. From mineral water, eleven resistant coliforms and one susceptible coliform were isolated. 2. All resistant isolates harbored diverse plasmids of ranged ca. 14-54kb. 3. Susceptible coliform harbored a only plasmid of ca. 2.8 kb. 4. All resistant isolates harbored common size of plasmid of ca. 14kb. 5. As a result of the transformation and agarose gel electrophoresis experiments, resistant factor was R-plasmid. In conclusion, It is suggested that heavy metal contamination of mineral water is the selective pressure for the plasmid encoding the tolerance. Heavy metal resistance, in some case, is present with antibiotic resistance. Therefore, heavy metal contamination of mineral water induces antibiotic resistant bacteria.

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A Studies on the Mineral Water Around Nam San (남산주변 약수의 수질에 관한 조사연구)

  • 최한영
    • Journal of Environmental Health Sciences
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    • v.15 no.1
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    • pp.75-79
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    • 1989
  • This study was carried out to investigate water quality of mineral water located in Nam san. 8 sites were pointed out by ramdom sampling and tested for 12 items in May and December 1988. The results were as follows: 1. The range of pH value in all sites was 5.4 - 6.8. 2. NH$_{3}$-N concentrations were suitable for drinking water criteria in winter season but site 1 was not for drinking water criteria. 3. NO$_{3}$-N concentrations were suitable for drinking water criteria in all samples. 4. Most of heavy metals were detected small amount, and espically zinc were detected much amount in all sites (0.01-0.32). 5. Coliform was not detected in all sites .in summer season but site 4 and 8 were detected in winter season.

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Growth Characteristics and Plasmid Profiles of Yersinia enterocolitica lsolated from Springs Water (약수터수로부터 분리한 Yersinia enterocolitica의 성장특성 및 Plasmid 유형)

  • 차인호;김미희;이상준
    • Journal of Life Science
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    • v.7 no.3
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    • pp.212-218
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    • 1997
  • The studies were conducted to explore the dffects of growth or survival against various factors and plasmid profiles of 49 Y. enterocolitica isolated from springs water. In the presence of calcium hypochlorite, y. enterocolitica was entirely extinguished by exposure for 33 hours at 0.8 ppm concentration, and was grown up to 7% NaCl, but not at 95 NaCI. Y. enterocolitica was presented optimal growth at pH 7.0 anad 9.0, and not allowth the growth at pH3.0, 5.0 and 11.0. The optimal temperature for growth of Y. enterocolitica was 25$\circ$C and 35$\circ$C, and allowed the growth at refrigerant temperature, 5$\circ$C. Y. enterocolitica was remarkably decreased by exposure for 30 seconds under UV light, and entirely extinguished by exposure for 90 seconds. Therefore, UV light was effective for sterilization of Y. enterocolitica. Fourty-nine strains of Y. enterocolitica were harbor plasmid DNA of approximately 46 Kb molecular weight.

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Information Flows, Differences of Opinion, and Trading Volumes : An Empirical Study (정보흐름, 의견차이, 거래량에 관한 실증연구)

  • Rhieu, Sang-Yup
    • Korean Business Review
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    • v.12
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    • pp.119-138
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    • 1999
  • In this study, we empirically investigate the relations between trading volumes and our proxies for information flows and differences of opnion. Econometric methods to analyze the relations in the equity and KOSPI 200 futures markets include Generalized Method of Moment(GMM) and Generalized Autoregressive Conditional Heteroscedasticity(GARCH) models. Major findings from our empirical analyses are summarized as follows; (i) Trading volume in both the equity and KOSPI 200 futures markets varies positively with proxies for information flows. We find that trading volumes in both markets are closely related to firm-specific information rather than market-wide information. (ii) Trading volumes in the equity and KOSPI 200 futures market have positive relations with our proxies for differences of opinion. (iii) Day-of-the-week effect is clear in both markets. Trading volumes in both the equity and KOSPI 200 futures markets tend to be relatively low early and late in the week. (IV) Futures contract life-cycle effect is clear. In other words, futures trading volume increses in the period around contract expiration. (V) In addition, ARCH effect on trading volumes is reported significant enough to take into account. The disturbance of trading volumes in both markets seem to be conditional heteroscedastic.

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