• KSBB Journal
• /
• 제8권2호
• /
• pp.122-125
• /
• 1993

Taxol은 최근 항암제로 각광을 받고 있는 물질로서 매우 복잡하고 고 기능성을 갖는 diterpene 알칼로이드이다. 본 연구에서는 국내의 덕유산, 소백산, 태백산 및 수원지역에서 서식하고 있는 주목나무들의 부위별 taxol 함량을 조사하였다. 그 결과 소백산에서 채취한 주목나무에서 가장 많은 taxol 양이 분석되었다. 부위별로는 껍질, 잔가지, 잎 순으로 taxol함량이 높게 나타났다.

• 생약학회지
• /
• 제37권1호통권144호
• /
• pp.60-66
• /
• 2006

Norditerpene alkaloids are the main toxic principles of traditional oriental herb 'chuawu(bu-shi),' which have been used in Chinese materia medica mainly for the treatment of musculoskeletal disorders. They are biosynthesized via cyclization of geranylgeranyl pyrophosphate, incoration of 2-aminoethanol and decarboxylation not via amino acid pathway, and the structural characteristics have tempted several oxidative reactions. In this study various norditerpene alkaloids were subjected to react active manganese dioxide and oxoammonium salt as oxidants. The oxidation was proceeded as dealkylation with aconitine type and dehydrogenations with lycoctonin and heteratisine type.

• 생약학회지
• /
• 제20권2호
• /
• pp.83-87
• /
• 1989

The alkaloids of thirty five Korean plants which belong to Rhamnaceae, Sterculiaceae, Celastracea, Urticaceae and Rubiaceae were screened. Plant was extracted and solvent fractionated to yield ether soluble alkaloidal fraction and butanol soluble fraction. Each fraction was subjected to alkaloidal test by Dragendorff's reaction. The result showed eighteen plants strong positive, fourteen weak and six negative to alkaloidal test.

• 생약학회지
• /
• 제39권2호
• /
• pp.127-134
• /
• 2008

From the under ground part of Aconitum volubile Pall. (Ranunculaceae),seven nor- or di-terpenoid alkaloids were isolated and identified by NMR spectra and mass spectrum. The alkaloids were three $C_{19}$-norditerpenoid alkaloids such as isotalatizidine, altaconitine and vilmorrianine A, and four $C_{20}$-diterpenoid alkaloids such as 12-epidehydronapelline, 12-epinapelline, songoramine and songorine. Some of the isolated diterpenoid alkaloids were subjected to oxidation reaction with active $MnO_2$ and oxoammonium salt. The results were C-12 oxidation(ketone formation) and N-dealkylation in veatchin-type and decomposition to non-alkaloidal compounds in atisine-type ajaconine.

• 생약학회지
• /
• 제49권2호
• /
• pp.108-112
• /
• 2018

Four quinolone alkaloids, 2-heptyl-4-quinolone (1), 2-nonyl-4-quinolone (2), 2-undecyl-4-quinolone (3), and 2-undecen-1'-yl-4-quinolone (4), together with two nitrogen derived benzoic acid derivatives, N-acetylanthranilic acid (5) and o-acetamidobenzamide (6) have been isolated from the Arctic bacterial strain, Pseudomonas aeruginosa. The structures of the compounds were determined by 1D and 2D NMR, and MS experiments, as well as by comparison of their data with published values. To the best of our knowledge, compounds 3-6 were isolated for the first time from P. aeruginosa.

• KSBB Journal
• /
• 제13권6호
• /
• pp.700-705
• /
• 1998

Studies were made to investigate effects of the scale-up of stirred tank bioreactors on cell growth and alkaloids production for suspension cultures of Eschscholtzia californica. In the 1.5 L STR, cell lysis was observed at 110 rpm or higher agitation speed. The agitation speed of 30 L STR was 43.7 rpm to maintain the same shear stress developed in 1.5 L STR of 100 rpm. As a result of scale-up from 1.5 L to 30 L STR, the specific growth rate was decreased from 0.12 to 0.07 day-1. The alkaloids productivity was also decreased from 0.24 to 0.14 mg/L-day. Changes of mixing performance and oxygen transfer were studied to explain the decrease of cell growth and alkaloids production. Decreased oxygen transfer rate coefficient(KLa) and increased mixing time by the scale-up was observed at various aeration rates.

• 약학회지
• /
• 제41권1호
• /
• pp.18-21
• /
• 1997

Synthesis of an optically active azabicyclo[3.2.1]octane skeleton as a backbone of the tropane alkaloids has been achieved by employing intramolecular Mannich reaction. Utilizat ion of (R)-${\alpha}$-methylbenzylamine as a chiral auxiliary provided an excellent cyclization of amino dioxolane precursor. However, this auxiliary did not afford high asymmertic induction for the preparation of the optically active cyclization precursor.

• 약학회지
• /
• 제45권1호
• /
• pp.34-38
• /
• 2001

The Phellodendri Cortex of Phellodendron amurense (Rutaceae) is known to contain a number of isoquinoline alkaloid, and berberine, palmatine, jateorrhizine, phellodendrine and magnoflorine are the major constituents of protoberberine alkaloids. For the determination of protoberberine alkaloids from Phellodendri Cortex and berberine chloride from the preparation, the new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate[II] complex ion. Samples were extracted with 0.1 mM hydrochloric acid, potassium biphthalate reagent, thiocyanatocobaltate reagent and 1.2-dichloroethane for 60 min. The absorbance of protoberberine alkaloid complexes in 1.2-dichloroethane solution was measured at 625 nm. Calibration curve for berberine was linear over the concentration range of 0.05~0.30 mg/ml 1.2-dichloroethane. The method proved to be rapid, simple and reliable for the determination of protoberberine alkaloids from Phellodendri Cortex and berberine chloride from the preparation.

• Journal of Ginseng Research
• /
• 제1권1호
• /
• pp.29-32
• /
• 1976

숙신산 탈수소효소의 활동성은 홍삼의 초산에틸 추출액과 초산에틸 추출모액의 수용 성 추출액 분획들에 의하여 활성화되었으며, 홍삼의 에테르 추출액 분획을 제외한 모든 분획들에 의하여 활성화되었다. 다른 사람들이 알칼로이드류는 숙신산 탈수소효소의 활동성을 증진시킨다는 암시와는 반대로 저자들의 연구결과는 오히려 인삼의 알칼로이프들은 이 효소의 활동성에 대하여 방해효과를 가졌음을 알려주었다.

• 약학회지
• /
• 제46권4호
• /
• pp.226-230
• /
• 2002

The Coptis Rhizome is known for containing a number of isoquinoline type alkaloids. Berberine, coptisine and palmatine are the major constituents of alkaloids. The alkaloids were isolated and determined by forming complex from Coptis japonica (Ranunculaceae). For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate[II] complex ion. The absorbance of alkaloidal complex in l,2-dichloroethane solution was measured at 625 nm. A calibration curve for the alkaloids isolated from Coptis Rhizome was linear over the concentration range of 0.2-0.3 mg/mι. The method proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in Coptis Rhizome.