Browse > Article

Isolation and Determination of Alkaloids in Coptis Rhizome by Forming Complex  

임소연 (우석대학교 약학대학)
김대근 (우석대학교 약학대학)
신태용 (우석대학교 약학대학)
임종필 (우석대학교 약학대학)
엄동옥 (우석대학교 약학대학)
Publication Information
YAKHAK HOEJI / v.46, no.4, 2002 , pp. 226-230 More about this Journal
Abstract
The Coptis Rhizome is known for containing a number of isoquinoline type alkaloids. Berberine, coptisine and palmatine are the major constituents of alkaloids. The alkaloids were isolated and determined by forming complex from Coptis japonica (Ranunculaceae). For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate[II] complex ion. The absorbance of alkaloidal complex in l,2-dichloroethane solution was measured at 625 nm. A calibration curve for the alkaloids isolated from Coptis Rhizome was linear over the concentration range of 0.2-0.3 mg/mι. The method proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in Coptis Rhizome.
Keywords
Coptis Rhizome alkaloids; isolation; determination; spectrophotometric method;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ishikawa, O., Hashimoto, T., Nakajima, T., Tanaka, O. and ltokawa, H. : Application of High-speed Liquid Chromatography to Analysis of Crude Drugs: Quaternary Alkaloids of Coptidis Rhizoma and Phellodendri Cortex. Yakugaku Zasshi, 98, 976 (1978)
2 Mura, T. and Tominaga, T. : Studies on the Quantitative Analysis by Thin Layer Chromatography II. On the Quantitative Analysis of Berberine in Coptidis Rhizoma. Syoyakugaku Zasshi, 27, 135 (1973)
3 Hashimoto, Y., Ando, K. and Mizuno, M.: Determination of Berberine in Crude Drugs by Fluorometric Densitometry. Syoyakugaku Zasshi, 30, 127 (1976)
4 El-Masry, S., Korany, M. A. and Abou-Donia, A. H. A. : Colorimetric and Spectrophotometric Determination of Hydrastis Alkaloids in Pharmaceutical Preparations. J. Pharm. Sci., 69, 597 (1980)
5 Yoneda, K., Yamagata, E., Miyaura, M., Longjin, H. and Mizuno, M. : Quantitative Analysis of Berberine type Alkaloids and Japanese Coptis Rhizome. Syoyakugaku Zasshi, 41, 205 (1987)
6 Namba, T.: The Encyclopedia pf Wakan-yaku (Traditional Sino-Japanese Medicines) with Color Picture, I, Hoikusha, p. 154 (1993)
7 Sakai, T. : Spectrophotometric determination of berberine by solvent extraction. Bunseki Kagaku, 24, 135 (1975)
8 Sakai, T. : Application of Thennochromism in Spectrophotometric Analysis: Selective Determinationof Berberinein Pharmaceuticals by Solvent Extraction. J. Pharm. Sci., 68, 875 (1979)
9 Sawda, T., Yamahara, J. and Shintani, Y. : The Assay of Berberine-type Alkaloids by Amberlite XAD-2. Syoyakugaku Zasshi, 28, 150 (1974)
10 Sawada, T., Yamahara, J., Ohashi, N. and Tutihashi, H. : Studies on the Evaluation of Crude Drugs by Bioassay (V). The Microbiological Assay for Berberine (3). Syoyakugaku Zasshi, 26, 12 (1972)
11 Sakai, T. : Spectrophotometric Determination of Trace Amounts of Quaternary Ammonium Salts in Drugs by Ion-pair Extraction with Bromphenol Blue and Quinine. Analyst, 108, 608 (1983)
12 Lee, H. S., Eom, Y. E. and Eom, D. O. : Narrowbore high performance liquid chromatography of berberine and palmatine in crude drugs and pharmaceuticals with ion-pair extraction using cobalt thiocyanate reagent. J Pharm Biomed Anal., 21, 59 (1999)
13 Ikuta, A., Kobayashi, A. and Itokawa, H. : Studies on the Quantitative Analysis of Protoberberine Alkaloids in Japanese, Chinese and Other Countries Coptis Rhizomes by Thin Layer Chromatograph-Densitometry. Syayakugaku Zasshi, 38, 279 (1984)
14 Philip, W. W. and Charles, G. D. : Nature of the Cobalt Thiocyanate Reaction. Anal. Chem., 23, 334 (1951)
15 Kimura, K, Noro, Y. and Handa, S.: Rapid Determination for Berberine-type Alkaloidsin Crude Drugs. A New Method with Sephades G-25 Column. Syoyakugaku Zasshi, 26, 141 (1972)
16 Misaki, T., Sagara, K., Ojima, M., Kakizawa, S., Oshima, T. and Yosihzawa, H. : Simultaneous Determination of Berberine, Palmatine and Coptisine in Crude Drugs and Oriental Pharmaceutical Preparations by Ion-Pair High-Performance Liquid Chromatography. Chem. Pharm. Bull., 30, 354 (1982)
17 Leonard, I. K. and Elizabeth, G. : Spectrophotometric Studies of Cobalt (II) Thiocyanate Complexes in Organic Solvents. J. Am. Chem. Soc., 72, 5659 (1950)
18 Hattori, T., Inoue, M. and Hayakawa, M. : Analytical Studies on the Active Constituents in Crude Drugs. I. Determination of Berberine in Coptidis Rhizoma with Tetrabromophenolphthalein Ethyl Ester using Thin-Layer Chromatography. Yakugaku Zasshi, 97, 1263 (1977)
19 Sawada, T., Yamahara, J. and Morika, E.: The Assay of Berberine-Alkaloids by CM-Sephadex (II); The Quantitative Analysis of Berberine in Crude Drug Preparations. Syoyakugaku Zasshi, 27, 106(1973)