• Title/Summary/Keyword: 아이섀도

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A Comparative Analysis of the Illumina Truseq Synthetic Long-read Haplotyping Sequencing Platform versus the 10X Genomics Chromium Genome Sequencing Platform for Haplotype Phasing and the Identification of Single-nucleotide variants (SNVs) in Hanwoo (Korean Native Cattle) (일루미나에서 제작된 TSLRH (Truseq Synthetic Long-Read Haplotyping)와 10X Genomics에서 제작된 The Chromium Genome 시퀀싱 플랫폼을 이용하여 생산된 한우(한국 재래 소)의 반수체형 페이징 및 단일염기서열변이 비교 분석)

  • Park, Woncheoul;Srikanth, Krishnamoorthy;Park, Jong-Eun;Shin, Donghyun;Ko, Haesu;Lim, Dajeong;Cho, In-Cheol
    • Journal of Life Science
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    • v.29 no.1
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    • pp.1-8
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    • 2019
  • In Hanwoo cattle (Korean native cattle), there is a scarcity of comparative analysis papers using highdepth sequencing and haplotype phasing, particularly a comparative analysis of the Truseq Synthetic Long-Read Haplotyping sequencing platform serviced by Illumina (TSLRH) versus the Chromium Genome Sequencing platform serviced by 10X Genomics (10XG). DNA was extracted from the sperm of a Hanwoo breeding bull (ID: TN1505D2184/27214) provided by Hanwoo research canter and used for the generation of sequence data from both the sequencing platforms. We then identified SNVs using an appropriate analysis pipeline tailored for each platform. The TSLRH and 10XG platforms generated a total of 355,208,304 and 1,632,772,004 reads, respectively, corresponding to a Q30 (%) of 89.04% and 88.60%, respectively, of which 351,992,768(99.09%) and 1,526,641,824(93.50%) were successfully mapped. For the TSLRH and 10XG platforms, the mean depth of the sequencing was 13.04X and 74.3X, the longest phase block was 1,982,706 bp and 1,480,081 bp, the N50 phase block was 57,637 bp and 114,394 bp, the total number of SNVs identified was 4,534,989 and 8,496,813, and the total phased rate was 72.29% and 87.67%, respectively. Moreover, for each chromosome, we identified unique and common SNVs using both sequencing platforms. The number of SNVs was directly proportional to the length of the chromosome. Based on our results, we recommend the use of the 10XG platform for haplotype phasing and SNV identification, as it generated a longer N50 phase block, in addition to a higher mean depth, total number of reads, total number of SNVs, and phase rate, than the TSLRH platform.

On the Persistence of Warm Eddies in the East Sea (동해 난수성 에디의 장기간 지속에 관하여)

  • JIN, HYUNKEUN;PARK, YOUNG-GYU;PAK, GYUNDO;KIM, YOUNG HO
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.24 no.2
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    • pp.318-331
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    • 2019
  • In this study, comparative analysis is performed on the long-term persisted warm eddies that were generated in 2003 (WE03) and in 2014 (WE14) over the East Sea using the HYCOM reanalysis data. The overshooting of the East Korea Warm Current (EKWC) was appeared during the formation period of those warm eddies. The warm eddies were produced in the shallow Korea Plateau region through the interaction of the EKWC and the sub-polar front. In the interior of the both warm eddies, a homogeneous water mass of about $13^{\circ}C$ and 34.1 psu were generated over the upper 150 m depth by the winter mixing. In 2004, the next year of the generation of the WE03, the amount of the inflow through the western channel of the Korea Strait was larger, while the inflow was lesser than its climatology during 2015 corresponding to the development period of the WE14. The above results suggest that the heat and salt are supplied in the warm eddies through the Tsushima Warm Current (TWC), however the amount of the inflow through the Korea Strait has negligible impact on the long-term persistency of the warm eddies. Both of the warm eddies were maintained more than 18 months near Ulleung island, while they have no common feature on the pathways. In the vicinity of the Ulleung basin, large and small eddies are continuously created due to the meandering of the EKWC. The long-term persisted warm eddies in the Ulleung Island seem to be the results of the interaction between the pre-existed eddies located south of the sub-polar front and fresh eddies induced by the meanderings of the EKWC. The conclusion is also in line with the fact that the long-term persisted warm eddies were not always created when the overshooting of the EKWC was appeared.

An Effects of Authentic Leadership and Transformational Leadership on Change Supportive Behavior in Small and Medium-size Business: Focused on Mediating Effect of Positive Psychological Capital (중소기업에서 상사의 변혁적 리더십과 진성 리더십이 변화지지행동에 미치는 영향: 긍정심리자본의 매개효과에 관한 연구)

  • Kim, Kyu Han;Huh, Chul Moo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.14 no.2
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    • pp.135-149
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    • 2019
  • Today, a growing number of companies are in trouble because leaders are lack of authentic leadership. Small and midium sized companies have the same issue. To correct the issue, there must be a real change in the relationship between managers and employees of the company as well as outside the company. In 21st century, authentic leadership is required. However, the research about determinants of change supportive behavior is not sufficient. Therefore, the purpose of this study is how manager's transformational leadership and authentic leadership affects change supportive behavior of employees in small and midium sized companies. The study also has to prove the role of positive psychological capital as a parameter. Data were collected from 424 employees working for small and midium sized companies in metropolitan area around Seoul and Gyeonggi. The data were analyzed using statistical package SPSS ver.21.0 and AMOS ver.18.0. Based on the research, First, the transformational leadership does not affect the change supportive behavior. However, authentic leadership affects the change supportive behavior. Also, positive psychological capital playes a role as parameter when transformational and authentic leadership, that are independent variable, affect change supportive behavior. On the other hand, the research shows that authentic leadership has more influence than transformational leadership on positive psychological capital. Transformational leadership without genuine attitude cannot affect change support behavior of employees in small and medium sized companies. Therefore, to affect change supportive behavior of employee, authentic leadership with genuine mind is required. After discussing the conclusions and implications of this study. the direction of the study for the follow - up study was suggested.

A Study on the Exhibition through the Web with Open Source Software OMEKA (공개 소프트웨어 OMEKA를 이용한 기록 웹 전시 방안 연구)

  • Choi, Yun-Jin;Choi, Dong-Woon;Kim, Hyung-Hee;Yim, Jin-Hee
    • The Korean Journal of Archival Studies
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    • no.42
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    • pp.135-183
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    • 2014
  • Korea has a high standard of IT environment to serve exhibit programs through the web with internet propagation and IT technology. However, the web exhibition of public institutions not only seem to introduce off-line exhibitions but also not to invigorate. It is caused by the lack of awareness, the cost of system installation and the lack of professional manpower. In this situation, OMEKA could suggest practical solutions to archives where need their own exhibition through the web. Especially, it would helpful for small record management organizations which are not enough budget and personal. OMEKA is an open source software program for digital collection and contents management. It has an affinity with users unlike traditional archives service programs. It also has been variously used by libraries, museums and schools because of exceptional exhibit functions. In this article, we introduce to the installation of a practical use about OMEKA. Regarding to OMEKA features, we consider it to raise exhibit effects. OMEKA would reduce the cost related to plans of exhibitions because it could display various contents and programs which reflecting characteristics of institutions. In addition, the availability of installation and widespread technological environment would lessen burden of public institutions. Using OMEKA, they would improve service level of public institutions and, make users satisfy. Therefore, they can change the social recognition of public institutions. OMEKA can contribute to various exercises of public records. It is not just the stereotypical system but, serves exhibition and collections with the strategy which each public institution would like to display. After all, it not only to connect to users with producers but also to improve the public image of institutions positively. Then, OMEKA would bring the great result through this interaction between public institutions and users.

'Hyangcheola', A New Fragrant Rice Variety with High Iron and Zinc Content (철과 아연 함량이 높고 향기가 있는 벼 '향철아')

  • Jeong, Eung-Gi;Ahn, Eok-Keun;Won, Yong-Jae;Lee, Jeong-Heui;Lee, Sang-Bok;Jeong, Jong-Min;Oh, Sea-Kwan;Choi, Yong-Hwan;Lee, Jeom-Ho;Suh, Jung-Pil;Hyun, Ung-Jo;Jung, O-Young;Kim, Bo-Kyeong;Son, Ji-Young
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.472-477
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    • 2018
  • "Hyangcheola" is a new japonica rice variety developed as a cross between "Joryeong" and "Seolhyangchal" by the rice breeding team at NICS in 2014. The heading date of "Hyangcheola" is July 28 in the middle plain area, which is 8 days earlier than that of "Hwaseong." "Hyangcheola" has a 76 cm long culm, which is 8 cm shorter than "Hwaseong" and has 86 spikelets per panicle. The viviparous germination rate of "Hyangcheola" was 27.9%. It showed resistance to stripe virus but susceptibility to bacterial blight (K1, K2, and K3 races), dwarf and black streak dwarf viruses and planthoppers. The milled rice of this variety exhibits translucent and medium short grain shape. The protein content of "Hyangcheola" was 8.2%, which was 1.5% higher than that of "Hwaseong." "Hyangcheola" is a rice with a fragrant aroma. The content of iron in 100 g of brown "Hyangcheola" rice was 15.12 mg, which is 3.69 mg higher than that of "Hwaseong," and the zinc content was 32.24 mg, which is 8.75 mg more than that of the comparative variety "Hwaseong." The average milled rice yield of "Hyangcheola" was 4.8 MT/ha at our sites under ordinary cultivation (Registration No. 6807).

'Samkwang1', a Medium Maturing, Multiple Disease Resistant, and High-quality Rice (중생 복합내병성 고품질 벼 '삼광1호')

  • Lee, Jeong-Heui;Won, Yong-Jae;Cho, Young-Chan;Lee, Jeom-Ho;Yang, Chang-Ihn;Kim, Myeong-Ki;Ahn, Eok-Keun;Suh, Jung-Pil;Lee, Sang-Bok;Jeon, Yong-Hee;Sung, Yeol-Kyu;Jeong, Eung-Gi;Ha, Woon-Goo;Chang, Jae-Ki;Jung, Kuk-Hyun;Yoon, Mi-Ra;Kang, Kyeong-Ho;park, Hyang-Mi;Roh, Jae-Hwan;Kim, Bo-Kyeong
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.490-496
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    • 2018
  • "Samkwang1," a japonica rice variety, was developed as a cross between "Samkwang" and F1 of Suwon152 (IT008283), which has a medium maturing and lodging resistance and Samkwang (IT284608), a high quality variety with bacterial blight resistance and mid-late maturing property by the rice breeding team at NICS in 2015. The heading date of "Samkwang1" was August 8 in the middle plain area, which was 2 days early than that of "Hwaseong." "Samkwang1" had a culm length of 77 cm, which was 7 cm shorter than that of "Hwaseong," and it had 128 spikelets per panicle. The viviparous germination rate of "Samkwang1" was 2.1%. "Samkwang1" showed resistance to blast, bacterial blight (K1, K2, and K3 race) and stripe virus, but was susceptible to the K3a race of bacterial blight, dwarf and black streak dwarf viruses, and plant hoppers. The milled rice of this variety exhibits translucent and medium short grains. The cooked rice grains of "Samkwang1" have an excellent palatability index (0.35) and lower protein content (6.2%) than that of "Hwaseong." The characteristics related to grain milling were better than those of "Hwaseong," especially the head rice milling recovery ratio and head rice ratio (94.2%). "Samkwang1" showed 5.62 MT/ha of milled rice productivity at 11 sites under ordinary cultivation conditions (Registration No. 6798).

Stage Structure and Population Persistence of Cypripedium japonicum Thunb., a Rare and Endangered Plants (희귀 및 멸종위기식물인 광릉요강꽃의 개체군 구조 및 지속성)

  • Lee, Dong-hyoung;Kim, So-dam;Kim, Hwi-min;Moon, Ae-Ra;Kim, Sang-Yong;Park, Byung-Bae;Son, Sung-won
    • Korean Journal of Environment and Ecology
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    • v.35 no.5
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    • pp.548-557
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    • 2021
  • Cypripedium japonicum Thunb. is an endemic plant in East Asia, distributed only in Korea, China, and Japan. At the global level, the IUCN Red List evaluates it as "Endangered Species (EN)," and at the national level in Korea, it is evaluated as "Critically Endangered Species (CR)." In this study, we investigated the characteristics of the age structure and the sustainability of the population based on the data obtained by demographic monitoring conducted for seven years in the natural habitat. C. japonicum habitats were observed in 7 regions of Korea (Pochoen, Gapyeong, Hwacheon, Chuncheon, Yeongdong, Muju, Gwangyang), and 4,356 individuals in 15 subpopulations were identified. The population size and structure differed from region to region, and artificial management had a very important effect on the size and structural change of the population. Population viability analysis (PVA) based on changes in the number of individuals of C. japonicum showed a very diverse tendency by region. And the probability of population extinction in the next 100 years was 0.00% for Pocheon, 10.90% for Gwangyang, 24.05% for Chuncheon, and 79.50% for Hwacheon. Since the above monitored study sites were located within the conservation shelters, which restricted access by humans, unauthorized collection of C. japonicum, the biggest threat to the species, was not reflected in the individual viability. So, the risk of extinction in Korea is expected to be significantly higher than that estimated in this study. Therefore, it is necessary to reflect population information in several regions that may represent various threats to determine the extinction risk of the C. japonicum population objectively. In the future, we should expand the demographic monitoring of the C. japonicum population known in Korea.

Estimation of cremation temperature on Baekje human bones from Seoul Seokchon-dong Ancient Tomb No.1 using XRD and FT-IR analysis (XRD와 FT-IR을 이용한 백제시대 옛사람 뼈의 화장(火葬) 온도 추정 - 서울 석촌동 고분군 1호 매장의례부 출토 옛사람 뼈를 중심으로 -)

  • YU, Jia;PARK, Serin;SHIN, Jiyoung
    • Korean Journal of Heritage: History & Science
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    • v.54 no.3
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    • pp.228-241
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    • 2021
  • Cremated archaeological bones provide crucial information to unravel the details of ancient cremation events and funeral culture. The research on cremated bones to date has been mainly focused on extracting archaeological information in Korea. Recently, the techniques of physical anthropology have been applied to obtain biological information and cremation temperatures for individuals. This study analyzed human remains excavated from Seoul Seokchon-dong Ancient Tombs and determined whether there were human cremations as well as the estimated cremation temperatures. There was no trace of fire in the pits where cremated bones were found; therefore, it is assumed that they were deposited with the artifacts. In this study, four samples were selected according to the surface color of the bones, and X-ray diffraction analysis (XRD) and Fourier-transformed infrared spectroscopy (FT-IR) were used for analysis. All four of the Seokchon-dong cremated bones were found, based on the crystallization index of X-ray diffraction analysis, to have experienced cremation above the temperature of 700℃. Infrared spectroscopy results indicated that the four bones from Seokchon-dong were cremated at temperatures above 700℃ and below 1,000℃. IR peaks were observed at 700℃, whereas no changes were found when bones experienced more than 1000℃. We assume that the cremated people in the Seokchon-dong Tombs were people of high-status because cremation at such a high temperature at that period required much effort. Here we present significant evidence of the cremation status and temperature of archaeological human bones on the basis of XRD and FT-IR, allowing for the restoration of the cremation events and funeral culture in archaeological sites.

Developing a Practical Course Model for the Personality Education in a Theological University (신학대학교에서 성품 교육을 위한 실천적 코스 모델 개발)

  • Yi, Miah
    • Journal of Christian Education in Korea
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    • v.66
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    • pp.75-112
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    • 2021
  • This study is purposed to prepare students in theological universities so that they contribute to their faith community as well as the broader social community in future through developing a practical course model for education of character formation. Facing the challenge of a high-tech revolution being progressed globally in this period, theological seminaries as the Christian-affiliated institute of higher education see the necessity to nurture their learners' competency of holistic personality comprehending cognitive, affective, behavioral, and more spiritual maturity as well as specialty in each subject matter. The holistic character capability of seminarians is closely related not only to their own self-realization, but also social community's well-being. This research had the following content and results. Chapter 1 brought up a subject to educational needs of the holistic character formation in the theological seminaries to meet the rapidly changing times. Chapter 2 considered the importance of character formation in the curriculum of the universities and theological seminaries in the times of high-tech revolution through the literature reviews related to. Chapter 3 clarified the elements of character education to be placed emphasis on theological seminaries and established the way of course planning. Chapter 4, using a course of Christian social education for illustration, described in order the whole process of course planning comprehending the need of planning, description of course concept, establishment and definition of learning outcomes, organization of learning experience(course content), teaching-learning activities, evaluation of learning outcome, and course improvement, which was visualized with the chart of the process of course design. Also, it provided a practical illustration of the conceptual map, an outline of content and an organization of units, and scope-sequence chart. Finally, chapter 5 gave an syllabus example in an integrative and digestive form as the course plan to be distributed to the students, which is the final result produced through the whole process of course planning. This research is intended not to present a overall curriculum planning of the university-level in the theological seminaries, but to develop a course-level curriculum focused on the formation of character. The author hope that this study would be used as a guideline in developing a course model to nurture the capability of the learners' holistic character.

Variation of Selected Phenotypic Characteristics, Anthocyanins and Bitter Sesquiterpene Lactones in Lettuce (Lactuca sativa L.) Germplasm (상추(Lactuca sativa L.)유전자원의 형태 특성 및 Anthocyanins과 Bitter Sesquiterpene Lactones 변이)

  • Choi, Susanna;Assefa, Awraris Derbie;Lee, Jae-Eun;Hur, On-Sook;Ro, Na-Young;Lee, Ho-Sun;Noh, Jae-Jong;Hwang, Ae-Jin;Kim, Yeong-Jee;Kim, Bich-Saem;Ko, Ho-Cheol;Rhee, Ju-Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.95-95
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    • 2019
  • 상추(Lactuca sativa L.)는 대표적인 쌈 및 샐러드 채소로 우리나라 기준(2016년) 3,387 ha의 면적에서 86,128톤을 생산하여 엽채류 중 배추, 양배추 다음으로 많이 생산되는 작물이다. 안토시아닌(Anthocyanins)은 열매, 꽃, 줄기, 잎 등 식물계에 널리 분포되어 있는 페놀 화합물 중 하나로 적색, 자색 등의 색을 나타내는 수용성 flavonoid계 색소이다. BSLs (Bitter sesquiterpene lactones)는 항암, 항균, 해열과 염증완화에 효과가 있는 것으로 알려져 있다. 본 연구는 농촌진흥청 농업유전자원센터에서 보유 중인 상추 66자원의 형태학적 특성 및 액체크로마토그래피(HPLC, UPLC)를 이용한 안토시아닌과 BSLs성분을 분석하여 함량이 높은 자원을 선발하고자 한다. 상추시료 0.05 g을 $MeOH/H_2O/AcAc$로 추출 한 후, UPLC를 사용하여 안토시아닌 함량을 분석하였으며, 상추시료 0.25 g을 100% MeOH로 추출 한 후 HPLC를 사용하여 BSLs 함량을 분석하였다. 연구 결과, 상추 유전자원의 안토시아닌 함량 범위는 0 mg/100 g에서 371.94 mg/100 g이고, BSLs성분 함량 범위는 $60.28{\mu}g/g\;DW$에서 $2821.92{\mu}g/g\;DW$ 이었다. 상추 66자원 중 안토시아닌함량이 200 mg/100 g이상인 자원은 IT217012, IT218395, IT231524, IT231525, IT260852이며, BSLs 함량이 $1700{\mu}g/g\;DW$이상인 자원은 IT231524, IT231525, IT231527, IT264971, IT271118이다. 두 성분의 함량이 모두 높은 자원 IT231524와 IT231525 이었다. 이 두자원의 형태적 특성은 초형이 잎상추로 잎이 넓은 타원형에 가장자리 결각이 강한 자주색이다. 따라서 본 연구는 다양한 상추 유전자원의 형태학적 특성 및 BSLs, 안토시아닌 성분이 높은 자원을 선발하여 육종소재로 활용하고자 한다.

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